Using an isotig encoding a putative polypeptide with high similarity to Arabidopsis LEA14 as a query,a 613 bp long cDNA was in silico cloned from the transcriptome data of rubber tree. The sequence nominated as HbLEA1...Using an isotig encoding a putative polypeptide with high similarity to Arabidopsis LEA14 as a query,a 613 bp long cDNA was in silico cloned from the transcriptome data of rubber tree. The sequence nominated as HbLEA14L2 contains an ORF of 456 bp with 3 bp 5' UTR and 154 bp 3' UTR. Subsequently,a 464 bp cDNA and an 834 bp genome sequence containing this ORF was amplified and sequenced. Sequence analysis suggested that HbLEA14L2 has one intron and encodes 151 amino acids with a theoretical molecular weight of 16. 55 kDa,isolectric point of 4. 93 and GRAVY value of- 0. 022,indicating a cytoplasmic localization pattern; HbLEA14L2 protein contains a conserved LEA_2 domain and belongs to LEA_2 subfamily,sharing 91%,76%,75%,72% and 63% similarity with the homologous proteins in castor bean,leafy spurge,poplar,cotton,and Arabidopsis,respectively. Swiss-Model indicated that the tertiary structure of HbLEA14L2 is consisted of one α-helix and seven β-sheets,which was proposed to serve as a regulatory protein to prevent cellular desiccation.展开更多
基金Supported by National Natural Science Foundation of China(31100460)Key Science and Technology Project of Hainan Province(90107)+1 种基金Natural Science Foundation of Hainan Province(312026)Fundamental Research Fund for the Rubber Research Institute in Chinese Academy of Tropical Agricultural Sciences(1630022011014)
文摘Using an isotig encoding a putative polypeptide with high similarity to Arabidopsis LEA14 as a query,a 613 bp long cDNA was in silico cloned from the transcriptome data of rubber tree. The sequence nominated as HbLEA14L2 contains an ORF of 456 bp with 3 bp 5' UTR and 154 bp 3' UTR. Subsequently,a 464 bp cDNA and an 834 bp genome sequence containing this ORF was amplified and sequenced. Sequence analysis suggested that HbLEA14L2 has one intron and encodes 151 amino acids with a theoretical molecular weight of 16. 55 kDa,isolectric point of 4. 93 and GRAVY value of- 0. 022,indicating a cytoplasmic localization pattern; HbLEA14L2 protein contains a conserved LEA_2 domain and belongs to LEA_2 subfamily,sharing 91%,76%,75%,72% and 63% similarity with the homologous proteins in castor bean,leafy spurge,poplar,cotton,and Arabidopsis,respectively. Swiss-Model indicated that the tertiary structure of HbLEA14L2 is consisted of one α-helix and seven β-sheets,which was proposed to serve as a regulatory protein to prevent cellular desiccation.
