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De Novo Transcriptome Analysis in Hevea brasiliensis to Unveil Genes Involved in Low Temperature Stress Response
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作者 Bingsong Yang Xiaomeng Shi +1 位作者 Tingting Guo Meixia Liang 《Phyton-International Journal of Experimental Botany》 SCIE 2023年第2期559-575,共17页
Low temperature is one of the adversities threatening the growth and development and reduces the yield of rubber trees.However,molecular mechanisms toward rubber trees in response to low temperature are largely unclea... Low temperature is one of the adversities threatening the growth and development and reduces the yield of rubber trees.However,molecular mechanisms toward rubber trees in response to low temperature are largely unclear.In this study,7,159 and 7,600 differentially expressed genes(DEGs)were identified in‘Reyan 73397’rubber trees.Through GO analysis,the catalytic activity was the representative of the GO term in the only DEGs at the two studied temperatures(room temperature and 4°C,respectively),while KEGG analysis showed that carbon metabolism was the most important grouping under the comparison of these two temperatures.In addition,expression of 9 members of transcription factor MYB family genes were further verified by qRT-PCR,and MYB family genes may play important roles in the regulation of rubber trees under low temperature stress.This study provided a theoretical foundation for(1)revealing the molecular mechanisms of rubber trees in response to low temperature and(2)breeding of tolerant varieties of rubber trees. 展开更多
关键词 Hevea brasiliensis low temperature stress TRANSCRIPTOME differential expressed genes
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Localized Effects of Mechanical Wounding and Exogenous Jasmonic Acid on the Induction of Secondary Laticifer Differentiation in Relation to the Distribution of Jasmonic Acid in Hevea brasiliensis 被引量:14
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作者 田维敏 史敏晶 +3 位作者 于凤义 吴继林 郝秉中 崔克明 《Acta Botanica Sinica》 CSCD 2003年第11期1366-1372,共7页
The relationship between the effect of exogenous jasmonic acid (JA) on the induction of secondary laticifer differentiation and the distribution of JA in the seedling of Hevea brasiliensis Mull. Arg. was investigated ... The relationship between the effect of exogenous jasmonic acid (JA) on the induction of secondary laticifer differentiation and the distribution of JA in the seedling of Hevea brasiliensis Mull. Arg. was investigated with the aid of experimental morphological and radioisotope technique. Most radioactivity of H-3-JA sustained in treated site within one hour while no radioactivity was detected in new shoot and the radioactivity in upper leaf was much less than that in the parts below the treated site, suggesting that JA was mainly transported downwards in the shoot of H brasiliensis. Mechanical wounding hindered the entrance of exogenous JA remarkably while held back the entered JA to the regions around wounded site. The effect of exogenous JA and mechanical wounding on the induction of the secondary laticifer differentiation was limited to treated site where high level of JA was expected. Mechanical wounding reduced the effect of exogenous JA on the differentiation of secondary laticifer, which could be ascribed to the hindrance of mechanical wounding to the entrance of exogenous JA. It was concluded from the combined data that a high accumulation of JA was required for inducing the secondary laticifer differentiation in H. brasiliensis. 展开更多
关键词 Hevea brasiliensis jasmonic acid mechanical wounding radioisotope technique laticifer differentiation
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Fluctuation of Microfibrillar Protein Level in Lutoids of Primary Laticifers in Relation to the 67 kD Storage Protein in Hevea brasiliensis 被引量:1
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作者 田维敏 韩亚琴 +1 位作者 吴继林 胡正海 《Acta Botanica Sinica》 CSCD 2003年第2期127-130,共4页
Abundant microfibrillar protein inclusions were present in the lutoids of the primary laticifers in Hevea brasiliensis Mull. Arg. Two forms of the inclusions could be distinguished under the electron microscope, each ... Abundant microfibrillar protein inclusions were present in the lutoids of the primary laticifers in Hevea brasiliensis Mull. Arg. Two forms of the inclusions could be distinguished under the electron microscope, each in separate lutoids. As revealed by SDS-PAGE, the 59.5 kD and 63.5 kD proteins were the major components of the microfibrillar protein purified by isoeleettic point precipitation. Western-blotting analysis indicated that they were immunorelated with the 67 kD protein accumulated in the protein-storing cells. The 59.5 kD and 63.5 kD proteins were abundant in the uppermost part, the stem of new shoot and sustained their abundance during the growth and development of new shoot while their contents decreased remarkably in the lower parts of the trunk, accompanying by the accumulation of 3-5 kinds of proteins with low molecular weights. This fluctuating pattern suggested that the degradation of the 59.5 kD and 63.5 kD proteins had nothing to do with the new shoot growth and may be closely related to the primary laticifer differentiation. The 67 kD protein could not be detected in the young stem of new shoot when its leaves were broze-colored while the protein started to be accumulated in the stem, when the leaves of new shoot had matured, behaving like a typical vegetative storage protein. 展开更多
关键词 microfibrillar protein 67 kD protein shoot growth laticifer development Hevea brasiliensis
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Effects of Micro-tapping with Different Concentration of Ethylene on Yield and Physiological Features of Rubber Tree(Hevea brasiliensis) Clone PR107
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作者 仇键 杨文凤 +3 位作者 吴明 魏芳 校现周 罗世巧 《Agricultural Science & Technology》 CAS 2015年第5期873-876,935,共5页
In this study, the rubber tree clone PR107 was selected as test material,and its yields and physiological features under micro-tapping with different concentrations of ethylene were compared. The results showed that t... In this study, the rubber tree clone PR107 was selected as test material,and its yields and physiological features under micro-tapping with different concentrations of ethylene were compared. The results showed that there was no significant correlation between rubber yield and ethylene concentration applied by microtapping on PR107. Too high level of ethylene was not conducive to improvement of rubber yield. When the ethylene concentration for micro-tapping was higher than60%, physical deficiencies appeared in rubber tree PR107, the dry rubber content was reduced, and the yield was unstable. Instead, when the ethylene concentration was lower than 40%, the physiological features of PR107 were steady, the dry rubber content was stable, and the yield was relatively high. 展开更多
关键词 Micro-tapping Physiological features of latex Ethylene concentration Hevea brasiliensis PR107
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Study on the Explant Disinfection of Root Culture of Hevea brasiliensis
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作者 姜泽海 周权男 李哲 《Agricultural Science & Technology》 CAS 2011年第4期514-516,共3页
[Objective] The aim was to look for an effective way of decreasing explants contamination in the root tissue culture of Hevea brasiliensis.[Method] In order to study root tissue culture of H.brasiliensis,new roots fro... [Objective] The aim was to look for an effective way of decreasing explants contamination in the root tissue culture of Hevea brasiliensis.[Method] In order to study root tissue culture of H.brasiliensis,new roots from forest section after treatment for one month were as explants,and 1 g/L carbendazim,75% ethanol,0.1% mercuric chloride and Yipeilong with different concentrations were used as disinfectants for roots disinfection.[Result] Before conventional disinfection,root explants were treated by 1 g/L carbendazim for 2.5 h,and disinfected by 75% ethanol for 30 s and 0.1% HgCl2 for 6 min,then cultured on the callus induction medium supplemented with 0.1% Yipeilong.The results showed that the contamination rate of explants decreased to 44.59%,and 25.60% explants survived after cultured for 30 d.[Conclusion] The study could provide theoretical foundation for the decrease of explant contamination in the root tissue culture of H.brasiliensis. 展开更多
关键词 Hevea brasiliensis Root explants Contamination control CARBENDAZIM Yipeilong
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Visualization Analysis of the Impact of Rubber Agroforestry Ecosystem on Soil Microbial Community
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作者 Jianan Liu Dongling Qi +5 位作者 Chuan Yang Zhixiang Wu Yingying Zhang Qingmao Fu Xianlei Jiang Ruxin Lin 《Advances in Bioscience and Biotechnology》 CAS 2024年第8期486-502,共17页
Rubber agroforestry systems positively impact soil microbial communities. This study employed a bibliometric approach to explore the research status, hotspots, and development trends related to these effects. Using Ci... Rubber agroforestry systems positively impact soil microbial communities. This study employed a bibliometric approach to explore the research status, hotspots, and development trends related to these effects. Using CiteSpace software, we visually analyzed research literature from the Web of Science (WOS) core database, spanning 2004 to 2024. The focus was on the impact of rubber agroforestry ecosystems on soil microbial communities. The results indicate significant attention from Chinese researchers, who have published numerous influential papers in this field. Authors Liu Wenjie have contributed the most papers, although no stable core author group exists. The Chinese Academy of Sciences is the leading research institution in terms of publication volume. While there is close collaboration between different institutions and countries, the intensity of researcher cooperation is low. The most cited literature emphasizes soil nutrients and structure in rubber agroforestry, laying a foundation for soil microorganism studies. Most cited journals are from countries like Netherlands and the United Kingdom. Key research areas include the effects of rubber intercropping on soil microbial communities, agroforestry management, and soil health. Research development can be divided into three stages: the initial stage (2010-2015), the development stage (2015-2020), and the mature stage (2020-2024). Current studies show that rubber intercropping and rubber-based agroforestry systems enhance soil microbial communities, positively impacting soil health. This paper provides a theoretical basis for the sustainable development of rubber agroforestry systems and improved management plans. Future research could explore the effects of species composition on soil microbiological characteristics and develop methods for species interactions. An in-depth study of the soil microbial community’s structure and function, and its relationship with rubber trees, is crucial. Developing effective, rationally designed rubber agroforestry systems and underground soil microbiome technology will promote sustainability and improve plantation productivity. 展开更多
关键词 Rubber (Hevea brasiliensis) Agroforestry Ecosystem MICROBE CiteSpace BIBLIOMETRICS Rubber Intercropping
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Molecular Cloning and Bioinformatical Analysis of a cDNA Encoding Mitochondrial 50S Ribosomal Protein L21 from Hevea brasiliensis Muell. Arg.
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作者 邹智 杨礼富 《Agricultural Science & Technology》 CAS 2012年第6期1203-1206,共4页
[Objective] "Tapping panel dryness (TPD)", a syndrome known as tapping incision blocked partly or entirely during latex exploiting, has become the most important factor causing great losses for rubber production. ... [Objective] "Tapping panel dryness (TPD)", a syndrome known as tapping incision blocked partly or entirely during latex exploiting, has become the most important factor causing great losses for rubber production. Aiming to elucidate the molecular mechanism of tapping panel dryness occurrence, this study carried out molecular cloning and bioinformatical analysis of a mRPL21 cDNA sequence, a gene associated with TPD. [Method] In a preliminary study, an expressed sequence tag (EST) encoding a deduced protein homologous to mitochondrial 50S ribosomal protein L21 (mRPL21), which showed to be down-regulated in the latex of TPD-affected rubber trees, was isolated by suppression subtractive hybridization (SSH). After ESTs assembling and RT-PCR validation, an 853 bp cDNA sequence with an open reading frame (ORF) was cloned, which was named as HbmRPL21 under GenBank accession number of HM230670. [Result] Bioinformatical analysis suggests that HbmRPL21 encodes a deduced polypeptide of 271 amino acids with a theoretical molecular weight (Mw) of 30.52 kDa and isolectric point (pI) of 8.40, and HbmRPL21 is a mitochondrion-targeted protein with a conserved domain of Ribosomal_L21p involving translation. Homology analysis reveals high amino acid sequence identity of mRPL21 from plants, while diversity of that between plant and animal kingdom. [Conclusion] This study laid the basis for further revealing the biological functions of mRPL21 in TPD-affected rubber trees. 展开更多
关键词 Hevea brasiliensis Tapping panel dryness syndrome Mitochondrial 50S ribosomal protein L21
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Evaluation of unconstrained and constrained mathematical functions to model girth growth of rubber trees (Hevea brasiliensis) using young agemeasurements 被引量:6
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作者 T.R.Chandrasekhar 《Journal of Forestry Research》 CAS CSCD 2012年第3期365-375,共11页
No attempt has been made to date to model growth in girth of rubber tree (Hevea brasiliansis). We evaluated the few widely used growth functions to identify the most parsimonious and biologically reasonable model fo... No attempt has been made to date to model growth in girth of rubber tree (Hevea brasiliansis). We evaluated the few widely used growth functions to identify the most parsimonious and biologically reasonable model for describing the girth growth of young rubber trees based on an incomplete set of young age measurements. Monthly data for girth of immature trees (age 2 to 12 yearsi from two locations were sub- jected to modelling. Re-parameterized, unconstrained and constrained growth functions,of Richards (RM), Gompertz (GM) and the monomo- lecular 'model ^(MM) were fitted to data. Duration of growth was the firsf constraint introduced. In the stagel We attempted a population aver- age (PA) model to capture the trend in growth. The best PA model was fitted as a subject specific (SS) model. We used appropriate error vari- ance-covariance structure to account for correlation due to repeated measurements over time. Unconstrainecl functions underestimated the asymptotic maximum that did not reflective carrying capacity of the locations. Underestimafions were attributed to the partial set' of meas- urements made during the early growth phase of the trees. MM proved superior to RM and GM. In the randomcoefficient models, both Gf and Go appeared to be influenced by tree level effects. Inclusion of diagonal definite positive matrix removed the correlation between random effects. The results were similar at both locations. In the overall assessment MM appeared as the candidate model for studying the girth-age relationships in Hevea trees. Based on the fitted model we conclude that, in Hevea trees, growth rate is maintained at maximum value at to, then decreases until the final state at dG/dt 〉 0, resulting in yield curve with no period of accelerating growth. One physiological explanation is that photosynthetic activity in Hevea trees decreases as girth increases and constructive metabolism is larger than destructive metabolism. 展开更多
关键词 natural rubber Hevea brasiliensis growth modelling un-constrained functions constrained functions mixed model
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Effect of natural and synthetic antioxidants on oxidative stability of FAMEs obtained from hevea brasiliensis 被引量:1
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作者 S.Nivetha D.Vetha Roy 《Journal of Energy Chemistry》 SCIE EI CAS CSCD 2013年第6期935-941,共7页
Biodiesel(fatty acid alkyl esters),an alternate to fossil fuel,has the tendency of autoxidation and hence requires antioxidants for long term storage.The influence of synthetic and natural antioxidants on the oxidativ... Biodiesel(fatty acid alkyl esters),an alternate to fossil fuel,has the tendency of autoxidation and hence requires antioxidants for long term storage.The influence of synthetic and natural antioxidants on the oxidative stability was analysed for fresh FAMEs(fatty acid methyl esters)obtained from hevea brasiliensis at 140 C.Higher activity was observed for synthetic antioxidants following the order of GA】BHT】DTBP】Q】GT-M】PH-M】GT-C,whereas the oxidative stability of stored FAMEs samples measured at 110 C reveals a nearly inverse trend.Storage stability was tested for the FAMEs obtained from hevea brasiliensis stored at 30 C,after addition of synthetic and natural antioxidants—butylated hydroxytoluene(BHT),2,6-di-tert-butylphenol(DTBP),quercetin(Q),gallic acid(GA),methanol extracts from green tea(GTM),pomegranate hull(PH-M),and chloroform extract of green tea(GT-C).Antioxidant activities above 1500 ppm was in the order of DTBP】BHT】GA】GT-C】GT-M】Q】PH-M.Synthetic antioxidants have been found more efficient to improve the storage stability of FAMEs obtained from hevea brasiliensis.DTBP in particular has the highest protection factor. 展开更多
关键词 hevea brasiliensis fatty acid methyl esters oxidative stability storage stability ANTIOXIDANTS
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Molecular Cloning and Analysis of LEA14L2 Gene from Rubber Tree( Hevea brasiliensis Muell. Arg. ) 被引量:1
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作者 Zhi ZOU Lifu YANG +2 位作者 Feng AN Zhenhui WANG Kun YUAN 《Agricultural Biotechnology》 CAS 2013年第5期4-8,共5页
Using an isotig encoding a putative polypeptide with high similarity to Arabidopsis LEA14 as a query, a 613 bp long cDNA was in silico cloned from the transeriptome data of rubber tree. The sequence nominated as HbLEA... Using an isotig encoding a putative polypeptide with high similarity to Arabidopsis LEA14 as a query, a 613 bp long cDNA was in silico cloned from the transeriptome data of rubber tree. The sequence nominated as HbLEA14L2 contains an ORF of 456 bp with 3 bp 5' UTR and 154 bp 3' UTR. Subsequently, a 464 bp eDNA and an 834 bp genome sequence containing this ORF was amplified and sequenced. Sequence analysis suggested that HbLEA14L2 has one intron and encodes 151 amino acids with a theoretical molecular weight of 16.55 kDa, isoleetric point of 4.93 and GRAVY value of -0.022, indicating a cytoplasmle localization pattern; HbLEA14L2 protein contains a conserved LEA_2 domain and belongs to LEA_2 subfamily, sharing 91%, 76%, 75%, 72% and 63% similarity with the homologous proteins in castor bean, leafy spurge, poplar, cotton, and Arabidopsis, respectively. Swiss-Model indicated that the tertiary structure of HbLEA14L2 is consisted of one α-helix and seven β-sheets, which was proposed to serve as a regulatory protein to prevent cellular desiccation. 展开更多
关键词 Hevea brasiliensis Muell. Arg. Arabidopsis thaliana LEA14 Shoot apex
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Intra-and inter-clonal tree growth variations of Hevea brasiliensis
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作者 H.R.Naji M.H.Sahri 《Journal of Forestry Research》 CAS CSCD 2012年第3期429-434,共6页
We evaluated the effects of planting densities (500, 1,000, 1,500 and 2,000 trees.ha-1) on tree growth performance (diameter at base, diameter at breast height, and clear bole height) of two clones (RRIM 2020 and... We evaluated the effects of planting densities (500, 1,000, 1,500 and 2,000 trees.ha-1) on tree growth performance (diameter at base, diameter at breast height, and clear bole height) of two clones (RRIM 2020 and PRIM 2025) of nine years old plantations of rubber tree (Hevea brasiliensis Muell. Arg) in Malaysia. For the four planting densities of the two clones, basal area and diameter at breast height declined with increasing planting density. Clear bole heights were greatest at 1,500 trees.haland lowest at 500 trees.ha-1 for the clone RRIM 2020, and at 2,000 trees.ha-1and 500 trees.halfor clone RRIM 2025. We conclude that the ideal planting density is 2,000 trees.ha"l for obtaining high volume of wood production and 500 trees-ha-1 for high wood quality. 展开更多
关键词 CLONE planting density tree growth Hevea brasiliensis
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Molecular Cloning and Sequence Analysis of an Aquaporin Gene Related to Tapping Panel Dryness in Rubber Tree( Hevea brasiliensis Muell. Arg. )
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作者 Zhi ZOU Yeyong MO +2 位作者 Dahua WANG Feng AN Lifu YANG 《Agricultural Biotechnology》 CAS 2014年第2期37-39,43,共4页
As a syndrome with tapping incision blocked partly or entirely during latex exploiting in rubber tree (Hevea brasiliensis ), "Tapping panel dryness (TPD)" causes great yield losses, thereby becoming the most imp... As a syndrome with tapping incision blocked partly or entirely during latex exploiting in rubber tree (Hevea brasiliensis ), "Tapping panel dryness (TPD)" causes great yield losses, thereby becoming the most important factor limiting rubber production. On the basis of an EST down-regulated in TPD-affected rubber trees, a 903 bp cDNA denoted HbPIP2;2 was isolated from the bark tissue with a combination of in silico cloning and RT-PCR. The cDNA contains an 867 hp ORF, 13 bp 5'UTR and 23 bp 3' UTR. Sequence analysis indicated that HbPIP2;2 encodes 288 amino acids with a theoretical molecular weight (Mw) of 30. 71 kDa and isolectric point (Pi) of 8.20. Bioinformatics analysis suggested that the deduced protein is predicted to have six transmembrane helices located to the plasma membrane and harbor one conserved MIP domain that can be grouped into plasma membrane intrinsic proteins (PIPs) of aquaporin (AQP) family. Homolo- gy search revealed that the protein shares a similarity of more than 90% with the homologues in Ricinus communis, Popttlus trichocarpa, Juglans regia and Theobro- ma cacao, suooorting a hie.hly conserved evolution. This study provided basis for further uncovering the regulatory role of AOPs in TPD occurrence. 展开更多
关键词 Hevea brasiliensis Tapping panel dryness AQUAPORIN Plasma membrane intrinsic protein
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Advances in Tissue Culture of Rubber( Hevea brasiliensis L. )
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作者 Mingwu LUO Liuhong DENG 《Agricultural Biotechnology》 CAS 2014年第6期34-37,共4页
Natural rubber, which is produced as a secondary plant metabolite, has been well recognized as an important raw industrial material due to its unique properties that can not be substituted by any alternative synthetic... Natural rubber, which is produced as a secondary plant metabolite, has been well recognized as an important raw industrial material due to its unique properties that can not be substituted by any alternative synthetic materials. Although more than 2000 plant species have been shown to possess the ability to synthe- size natural rubber, Hevea brasiliensis L. is yet the only one qualified as a commercial source for natural rubber production, which not only has important economic value, but "also leads to significant ecological and social benefits to maintain ecological forest microclimate and ease current ecological issues caused by dwindling natural forests. With the growing demand for natural rubber, providing a large number of high-quality rubber planting materials has become a major issue to be solved. In this paper, advances on tissue culture of H. brasiliensis were summarized, which laid the foundation for breeding high-quality rubber planting materials. 展开更多
关键词 Hevea brasiliensis L. Tissue culture ADVANCES
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Molecular Cloning and Sequence Analysis of a Tonoplast Aquaporin Gene TIP1 Associated with Tapping Panel Dryness in Hevea brasiliensis
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作者 Zhi ZOU Danhua WANG +2 位作者 Yeyong MO Feng AN Lifu YANG 《Agricultural Biotechnology》 CAS 2014年第3期14-17,共4页
[Objective]To address the role of aquaporins(AQPs)in the occurrence of Tapping Panel Dryness(TPD),a phenomenon with tapping incision blocked partly or entirely during latex exploiting in the rubber tree(Hevea bra... [Objective]To address the role of aquaporins(AQPs)in the occurrence of Tapping Panel Dryness(TPD),a phenomenon with tapping incision blocked partly or entirely during latex exploiting in the rubber tree(Hevea brasiliensis)that causes great losses on rubber production,a tonoplast aquaporin gene associated with TPD occurence was cloned and analyzed.[Method]Based on an EST down-regulated in TPD-affected rubber trees,a 774 bp cDNA designated HbTIP1 was isolated from the bark tissue of Hevea brasiliensis with a combination of in silico cloning and RT-PCR.And the gene structure and sequence characteristics were analyzed using bioinformatics tools.[Result]The cDNA includes a 759 bp ORF,8 bp 5’UTR and 7 bp 3’UTR.Sequence analysis indicated that HbTIP1putatively encodes 252 amino acids with a theoretical molecular weight(Mw)of 25.88 kDa and isolectric point(pI)of 4.96.Bioinformatics analysis suggested that the deduced protein is predicted to have six transmembrane helices located to the vacuolar membrane and to harbor one conserved MIP domain that can be grouped into the tonoplast intrinsic proteins(TIPs)of aquaporin family.Homology search revealed that the protein shares a similarity of more than 90%with the homologues in Theobroma cacao,Prunus persica,Citrus sinensis and Ricinus communis,supporting a highly conserved evolution.[Conclusion]This study provided basis for further revealing the regulatory role of AQPs in the TPD occurrence. 展开更多
关键词 Hevea brasiliensis Tapping panel dryness AQUAPORIN
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Exploration and Practice of Rubber Based Agroforestry Complex Systems in China
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作者 Dongling Qi Zhixiang Wu +4 位作者 Chuan Yang Zhongliang Tao Linlin Zhao Yingying Zhang Qingmao Fu 《Advances in Bioscience and Biotechnology》 2023年第12期479-491,共13页
Agroforestry ecosystems are constructed by simulating natural ecosystems, applying the principles of symbiosis in nature, and organizing multiple plant populations to coexist, while conducting targeted cultivation and... Agroforestry ecosystems are constructed by simulating natural ecosystems, applying the principles of symbiosis in nature, and organizing multiple plant populations to coexist, while conducting targeted cultivation and structural control scientifically. Rubber agroforestry complex ecosystems aim for sustainable development in terms of industry, ecology, resource utilization, and the livelihoods of producers. Rubber agroforestry complex ecosystems create a complex production structure system that integrates biology, society, and the economy through species combinations. Rubber trees and associated biological components coordinate with each other, mutually promote growth, and yield a variety of products for producers. Cultivation techniques and patterns of rubber agroforestry are essential components of these ecosystems. This study analyzes the production practices of rubber agroforestry complex cultivation, with a focus on the development and characteristics (complexity, systematicity, intensity, and hierarchy) of rubber agroforestry systems using a literature analysis and a survey approach. It explores the types and scales of complex planting, specifications and forms, and major effects of complex cultivation. This study identifies successful rubber agroforestry cultivation patterns and practical techniques, as well as the potential benefits of developing rubber agroforestry cultivation. It also points out the shortcomings in the development of complex planting, including an emphasis on production practices but insufficient theoretical research, a focus on production but inadequate attention to the market, and an emphasis on yield while overlooking the improvement of standards, brands, and added value. There are various complex patterns for young rubber plantations, but relatively fewer for mature plantations. Based on this analysis, this study suggests that future efforts should focus on in-depth research on interspecies and environmental interactions in rubber agroforestry ecosystems, clearly define key roles, accelerate the innovation of development patterns, and strengthen the foundation for development. It recommends promoting and demonstrating successful rubber agroforestry complex patterns and providing technical training, developing product branding for rubber agroforestry patterns, enhancing product value, expanding the application functions of rubber-forest mixed crop products, and establishing a stable and sustainable industry chain. This study provide practical experience and theoretical insights in rubber agroforestry complex systems from China the potential to enrich the knowledge of rubber agroforestry composite systems, provide practical experience to improve the operating income of smallholders, and even promote the sustainable development of rubber plantations. 展开更多
关键词 Rubber Tree (Hevea brasiliensis) Agroforestry Ecosystem Rubber Intercropping Complex Ecological Cultivation Land Resource
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Characteristics of Soil Organic Carbon and Total Nitrogen in Rubber Plantations Soil at Different Age Stages in the Western Region of Hainan Island 被引量:17
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作者 吴志祥 谢贵水 +2 位作者 陶忠良 周兆德 王旭 《Agricultural Science & Technology》 CAS 2010年第1期147-153,共7页
[Objective]The aim was to study on the characteristics of soil organic carbon and nitrogen in rubber (Hevea brasiliensis Muell-Arg) plantations at different age stages in the western region of Hainan Island,so as to... [Objective]The aim was to study on the characteristics of soil organic carbon and nitrogen in rubber (Hevea brasiliensis Muell-Arg) plantations at different age stages in the western region of Hainan Island,so as to evaluate the ecological benefits of rubber plantations and provide basic data for studying the effect of tropical land utilization/cover change on the global carbon and nitrogen cycle. [Method]The situs was in Danzhou city,western region of Hainan Island,and the samples were four kinds of rubber plantations soil at different ages and one kind of control check (pepper,Piper nigrum L.) soil. In this research,four quadrats were set up in each sample,and the size of each was 20 cm×20 cm. Four specimens were gathered from four layers of 0-15,15-30,30-45,45-60,and the average of them was the last analysis result of each sample. Soil density was measured by cutting ring method,soil containing and hygroscopic water was detected by oven drying method,soil organic carbon (SOC) was measured by low temperature heated outside potassium dichromate oxidation-colorimetry method,and soil total nitrogen (STN) was detected by semimicro Kjeldahl method. [Result]SOC contents of different layers in rubber plantations soil at different age stages (including the CK pepper soil,the same as below) varied little,and the content of SOC in surface layer (0-15 cm) was higher,while the underlayer (45-60 cm) was lower than the average value; there was significant difference in SOC content among different kinds of soil,and the content was of 6.03-7.78 g/kg,tapping young trees (7 years) CK pepper mature age trees (30 years) prophase of young trees (2 years) tapping trees (16 years); there was no significant difference in SOC storage among different kinds of soil,and the storage was of 61.33-74.29 t/hm2,mature age trees (30 years) tapping young trees (7 years) prophase of young trees (2 years) CK pepper tapping trees (16 years); there was significant difference in STN content among rubber plantations soil at different age stages,the content was of 410.86-664.14 mg/kg2,CK pepper tapping young trees (7 years) prophase of young trees (2 years) mature age trees (30 years) tapping trees (16 years),and STN content of tapping trees (16 years) soil was extremely lowest; there was significant difference in C/N ratio among different kinds of soil,the ratio was of 10.94-14.47,and the ratio of tapping trees (16 years) mature age trees (30 years) tapping young trees (7 years) CK pepper prophase of young trees (2 years). [Conclusion]There wasn't unhealthy effect of rubber trees planted in tropical area on the content and storage of SOC,the content of STN and the ratio of C/N. there was no significant difference between rubber plantations and CK pepper soil,and the effects of rubber plantation on soil carbon-nitrogen was similar to that of other tropical crops (such as pepper). 展开更多
关键词 Different age stages of trees Rubber (Hevea brasiliensis plantations Soil organic carbon (SOC) Soil total nitrogen (STN) Hainan Island
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The rapid determination of volatile fatty acid number in para rubber latex using fourier transform-near infrared spectroscopy based on quantification and discrimination model
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作者 Sureeporn Narongwongwattana Ronnarit Rittiron Chin Hock Lim 《Journal of Innovative Optical Health Sciences》 SCIE EI CAS 2015年第5期107-115,共9页
Volatile Fatty Acid number(VFA no.)is one of the parameters indicating the state of quality of Para rubber latex at that particular time.Most factories analyze this parameter using standard analytical method as in ISO... Volatile Fatty Acid number(VFA no.)is one of the parameters indicating the state of quality of Para rubber latex at that particular time.Most factories analyze this parameter using standard analytical method as in ISO 506:1992(E).Nevertheless,this procedure is complicated,chemical and time consuming,as well as skilled analyst required.Therefore,near infrared(NIR)spectroscopy which is rapid,accurate and nonchemicals method was applied to determine the VFA no.infield latex and concentrated latex based on quantification and discriminant model.The best calibration equation was obtained from standard normal variate(SNV)spectra in the region of 6109.7–5770.3,4613.1–4242.9 cm^(-1) with R?0:832,SECV?0:036 and no bias.From the performance check,statistically it was found that SECV and bias were low enough for practical acceptance and the predicted VFA no.was not di®erent significantly from actual VFA no.at 95% confidence intervals.In addition,discriminant model was developed to separate good quality latex from the deteriorated latex using VFA no.at 0.06 as standard as in ISO 2004:2010(E).The discriminant model can be used to screen the latex with overall accuracy of 91.86%in validation set. 展开更多
关键词 Hevea brasiliensis latex quality POLYISOPRENE ISO 12099:2010(E)
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Weed Control Strips Influences on the Rubber Tree Growth
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作者 Caio Doria Guzzo Leonardo Bde Carvalho +2 位作者 Pedro Luis da CAAlves Elaine Cristine Piffer Goncalves Paulo FGiancotti 《American Journal of Plant Sciences》 2014年第8期1059-1068,共10页
Hevea brasiliensis (rubber tree) is the most widely cultivated tree species for producing natural rubber latex whose productivity can be reduced by weeds. Field experiment was carried out during two years to evaluate ... Hevea brasiliensis (rubber tree) is the most widely cultivated tree species for producing natural rubber latex whose productivity can be reduced by weeds. Field experiment was carried out during two years to evaluate the growth of rubber tree plants submitted to different weed control strips (0-weed-free, 50, 100, 150, 200, 250, 300, 350, and 400-(weedy cm) and to determine the critical width of weed control strip during the establishment of rubber tree plantation. Weed community was composed mainly by Urochloa decumbens (90%). The width of the weed control strip influenced on the initial plant growth of rubber trees, reducing the plant height, stem diameter, leaf area, and aboveground dry mass accumulation. In consequence, crop plantation was affected by the width of weed control. The critical width of the weed control strip for establishment of a rubber tree plantation was within 100 cm. 展开更多
关键词 Hevea brasiliensis Urochloa decumbens Weed Control Weed Interference
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Divergent DNA Methylation Patterns Associated with Abiotic Stress in Hevea brasiliensis 被引量:8
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作者 Thomas K. Uthup Minimol Ravindran, K. Bini Saha Thakurdas 《Molecular Plant》 SCIE CAS CSCD 2011年第6期996-1013,共18页
Cytosine methylation is a fundamental epigenetic mechanism for gene-expression regulation and development in plants. Here, we report for the first time the identification of DNA methylation patterns and their putative... Cytosine methylation is a fundamental epigenetic mechanism for gene-expression regulation and development in plants. Here, we report for the first time the identification of DNA methylation patterns and their putative relationship with abiotic stress in the tree crop Hevea brasiliensis (source of 99% of natural rubber in the world). Regulatory sequences of four major genes involved in the mevalonate pathway (rubber biosynthesis pathway) and one general defense-related gene of three high-yielding popular rubber clones grown at two different agroclimatic conditions were analyzed for the presence of methylation. We found several significant variations in the methylation pattern at core DNA binding motifs within all the five genes. Several consistent clone-specific and location-specific methylation patterns were identified. The differences in methylation pattern observed at certain pivotal cis-regulatory sites indicate the direct impact of stress on the genome and support the hypothesis of site-specific stress-induced DNA methylation. It is assumed that some of the methylation patterns observed may be involved in the stress-responsive mechanism in plants by which they adapt to extreme conditions. The study also provide clues towards the existence of highly divergent phenotypic characters among Hevea clones despite their very similar genetic make-up. Altogether, the observations from this study prove beyond doubt that there exist epigenetic variations in Hevea and environmental factors play a significant role in the induction of site-specific epigenetic mutations in its genome. 展开更多
关键词 DNA methylation EPIGENETICS abiotic stress rubber biosynthesis Hevea brasiliensis.
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In vitro Transient Expression System of Latex C-serum was used for Analysis of Hevein Promoter in Response to Abscisic Acid in Hevea brasiliensis
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作者 Xiao-Wen Fei Xiao-Dong Deng 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2008年第3期338-344,共7页
Hevein has been found to be an essential element in coagulation of rubber particles in latex of rubber trees. In a previous study, we cloned a 1 241-bp fragment of a 5' upstream region of the hevein gene by genome wa... Hevein has been found to be an essential element in coagulation of rubber particles in latex of rubber trees. In a previous study, we cloned a 1 241-bp fragment of a 5' upstream region of the hevein gene by genome walking. This fragment was analyzed by a 5' end nested deletion method in the present study, fused with a uidA (gus) gene to produce a series of tested constructs, which were transferred into C-serum of latex and the Gus activities were detected. Results showed that the fragment from -749 to -292 was sufficient for expression of gus gene in latex, and the fragment from -292 to -168 was crucial in response to abscisic acid inducement. In a transient transgenic test of rubber leaf with particle bombardment, construct Hev749 conferred gus-specific expression in veins, in which the latex tubes mainly distributed. This implies that the fragment from -749 to -292 was laticiferous-specific. 展开更多
关键词 abscisic acid gus (beta-glucuronidase) gene hevein promoter rubber tree (Hevea brasiliensis
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