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前期处理对西瓜花药培养愈伤组织诱导的影响 被引量:5
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作者 朱迎春 孙德玺 +3 位作者 邓云 安国林 李卫华 刘君璞 《中国瓜菜》 CAS 2012年第5期17-19,共3页
以6份不同基因型西瓜材料为试材,研究了HgCl2消毒处理时间和低温预处理时间对西瓜花药培养愈伤组织诱导的影响。结果表明:用75%酒精处理30s,再用0.1%的HgCl2消毒8min时,西瓜花药污染率和褐化率均较低,可作为西瓜花药培养的主要消毒方法;... 以6份不同基因型西瓜材料为试材,研究了HgCl2消毒处理时间和低温预处理时间对西瓜花药培养愈伤组织诱导的影响。结果表明:用75%酒精处理30s,再用0.1%的HgCl2消毒8min时,西瓜花药污染率和褐化率均较低,可作为西瓜花药培养的主要消毒方法;在4℃预处理48、72h时,西瓜花药培养愈伤组织诱导率较高,均显著高于对照,说明4℃低温预处理可作为花蕾的一种保存方式;基因型间愈伤组织诱导率差异显著,1005愈伤组织诱导率最高。 展开更多
关键词 西瓜 花药培养 低温预处理 消毒处理 愈伤组织诱导
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Study on Response Rule of the Microcystis aeruginosa Fluorescence on the Biological Toxicity of HgCl_2
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作者 CUI Jian-sheng QI Cai-ya LIU Xiao-jing 《Meteorological and Environmental Research》 2012年第10期20-22,26,共4页
[ Objective] The research aimed to study response rule of the M. aeruginosa fluorescence on the biological toxicity of HgCI2. [ Method ] M. aeruginosa as material, fluorescence intensity at its best excitation and emi... [ Objective] The research aimed to study response rule of the M. aeruginosa fluorescence on the biological toxicity of HgCI2. [ Method ] M. aeruginosa as material, fluorescence intensity at its best excitation and emission wavelengths as measured indicator, influence of the HgCI2 at different mass concentrations on fluorescence intensity of the M. aeruginosa was discussed initially. [ Result] HgCI2 at different mass concentrations had different influences on M. aeruginosa. HgCI2 at low concentration (0.002 -0.004 mg/L)could promote photosynthesis of the M. aeruginosa. It showed as fluorescence value of the algae liquid becoming smaller. 0.010 -0.400 mg/L of HgCI2 inhibited photosynthesis of the M. aeruginosa. It showed as fluorescence value of the algae liquid becoming bigger. Moreover, inhibition effect increased as HgCI2 concentration rose, showing a positive correlation between HgCI2 concentration and toxicity ( R 2 = 0.963 5 ). [ Conclusion ] The research provided new theoretical basis for quickly measuring water toxicity. 展开更多
关键词 M. aeruginosa FLUORESCENCE hgci2 Biological toxicity Response rule China
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