AIM:To explore the effect of co-host non-coding RNA(ncRNA)MIR503HG/miR-503-5p on the angiogenesis of pterygium.METHODS:MIR503HG/miR-503-5p/fibroblast growth factor 2(FGF2)expression levels in pterygium tissues,control...AIM:To explore the effect of co-host non-coding RNA(ncRNA)MIR503HG/miR-503-5p on the angiogenesis of pterygium.METHODS:MIR503HG/miR-503-5p/fibroblast growth factor 2(FGF2)expression levels in pterygium tissues,control conjunctival tissues,and human pterygium fibroblasts(HPF)were examined by reverse transcription-polymerase chain reaction(qRT-PCR)and immunohistochemical methods.Effects of MIR503HG/miR-503-5p on low molecular weight FGF2(LWM FGF2),migration and angiogenesis of human retinal microvascular endothelial cells(HRMEC)were determined in an HPF and HRMEC co-culture model using Western blots,wound healing assay,Matrigel-based tube formation assay,and Transwell assay.RESULTS:MIR503HG/miR-503-5p/FGF2 pathway was actively increased in pterygium tissue and there was a negative correlation between the expression of the two ncRNAs.FGF2 expression level was positively correlated with MIR503HG and negatively correlated with miR-503-5p.Overexpressed MIR503HG/miR-503-5p did not affect the migration and angiogenesis of HRMECs cultured separately,but significantly affected migration and angiogenesis of HRMEC in HPF and HRMEC co-culture models.Western blotting revealed that MIR503HG/miR-503-5p overexpression significantly increased LMW FGF2 expression in HPF.CONCLUSION:MIR503HG/miR-503-5p inhibits HRMEC migration and angiogenic function by interfering with the interaction between HPF and endothelial cells via reducing LMW FGF2 in HPF.展开更多
活动断裂带能够向大气释放一定量的温室气体及有害气体(如CO_2,Rn,Hg等),对大气环境产生一定的影响.于2012年5月和2013年5月对首都圈西北部9条活动断裂带CO_2、Rn和Hg的脱气浓度及通量进行了测量,并计算了CO_2和Hg脱气对大气的年贡献量...活动断裂带能够向大气释放一定量的温室气体及有害气体(如CO_2,Rn,Hg等),对大气环境产生一定的影响.于2012年5月和2013年5月对首都圈西北部9条活动断裂带CO_2、Rn和Hg的脱气浓度及通量进行了测量,并计算了CO_2和Hg脱气对大气的年贡献量.结果表明:(1)活动断裂带土壤气中CO_2、Rn和Hg浓度的平均值分别为0.58%、8.88 k Bq·m-3和6.44 ng·m-3,其浓度的最大值分别为9.47%、79.1 k Bq·m-3和105 ng·m-3;土壤气浓度整体具有从西向东,自南向北增大的趋势.断裂带CO_2、Rn和Hg通量的平均值分别为55.7 g·m-2·d-1、55.87 m Bq·m-2·s-1和6.78 ng·m-2·h-1,其通量的最大值分别为274.29 g·m-2·d-1、230.22 m Bq·m-2·s-1和74.33 ng·m-2·h-1.(2)京西北活动断裂带多数测区土壤气CO_2浓度无窒息风险;部分测区土壤气Hg浓度大于38 ng·m-3,属中度污染区;首都圈西北部9条活动断裂带通过脱气作用对大气CO_2的年贡献量为2 Mt,对大气Hg的年贡献量为5.3 kg;活动断裂带部分测点氡浓度较高,其附近建筑物应采取综合防氡措施.展开更多
This study was performed to estimate the emission of non-CO 2 greenhouse gases(GHGs) from biomass burning at a large fire area.The extended methodology adopted the IPCC Guidelines(2003) equation for use on data from t...This study was performed to estimate the emission of non-CO 2 greenhouse gases(GHGs) from biomass burning at a large fire area.The extended methodology adopted the IPCC Guidelines(2003) equation for use on data from the Samcheok forest fire gathered using 30 m resolution Landsat TM satellite imagery,digital forest type maps,and growing stock information per hectare by forest type in 1999.Normalized burn ratio(NBR) technique was employed to analyze the area and severity of the Samcheok forest fire that occurred in 2000.The differences between NBR from pre-and post-fire datasets are examined to determine the extent and degree of change detected from burning.The results of burn severity analysis by dNBR of the Samcheok forest fire area revealed that a total of 16,200 ha of forest were burned.The proportion of the area characterized by a 'Low' burn severity(dNBR below 152) was 35%,with 'Moderate'(dNBR 153-190) and 'High'(dNBR 191-255) areas were at 33% and 32%,respectively.The combustion efficiency for burn severity was calculated as 0.43 for crown fire where burn severity was 'High',as 0.40 for 'Moderate' severity,and 0.15 for 'Low' severity surface fire.The emission factors for estimating non-CO 2 GHGs were separately applied to CO 130,CH 4 9,NO x 0.7 and N 2 O 0.11.Non-CO 2 GHGs emissions from biomass burning in the Samcheok forest fire area were estimated to be CO 44.100,CH 4 3.053,NO x 0.238 and N 2 O 0.038 Gg.展开更多
芝麻是八大类食物过敏原之一,快速准确识别芝麻过敏原对预防其过敏有重要意义。核酸适配体可以高效识别靶标过敏原,在过敏原检测中有良好的应用前景。为了获得芝麻主要过敏原Ses i 2的特异性核酸适体,本研究以Ses i 2为靶标,通过磁珠筛...芝麻是八大类食物过敏原之一,快速准确识别芝麻过敏原对预防其过敏有重要意义。核酸适配体可以高效识别靶标过敏原,在过敏原检测中有良好的应用前景。为了获得芝麻主要过敏原Ses i 2的特异性核酸适体,本研究以Ses i 2为靶标,通过磁珠筛选法(磁珠-SELEX)开展10轮筛选,经由高通量测序获得6条候补序列(S1~S6),并进行家族性、同源性分析及二级结构预测。结果表明,6条候选核酸适体的重复率可达46.38%,其自由能在-9.02到-2.47 kcal·moL^(-1)之间,根据自由能能量稳定原则,S1和S5吉布斯自由能最低最稳定,分别为-6.70和-9.02 kcal·moL^(-1)。利用ELISA试验进行亲和力测试,结果表明核酸适体S1和S2的亲和能力较强,S1:KD=67.02 nmol·L^(-1),R2=0.925 8,S2:KD=97.65 nmol·L^(-1),R2=0.795 1。核酸适体S1与过敏原Ses i 2的结合力和其他过敏原蛋白相比有显著差异,可视为具有特异性。本研究最终获得一条兼具良好亲和力和特异性的核酸适体S1,为芝麻过敏原快速检测提供了技术支撑。展开更多
基金Supported by the National Natural Science Foundation of China(No.81770898).
文摘AIM:To explore the effect of co-host non-coding RNA(ncRNA)MIR503HG/miR-503-5p on the angiogenesis of pterygium.METHODS:MIR503HG/miR-503-5p/fibroblast growth factor 2(FGF2)expression levels in pterygium tissues,control conjunctival tissues,and human pterygium fibroblasts(HPF)were examined by reverse transcription-polymerase chain reaction(qRT-PCR)and immunohistochemical methods.Effects of MIR503HG/miR-503-5p on low molecular weight FGF2(LWM FGF2),migration and angiogenesis of human retinal microvascular endothelial cells(HRMEC)were determined in an HPF and HRMEC co-culture model using Western blots,wound healing assay,Matrigel-based tube formation assay,and Transwell assay.RESULTS:MIR503HG/miR-503-5p/FGF2 pathway was actively increased in pterygium tissue and there was a negative correlation between the expression of the two ncRNAs.FGF2 expression level was positively correlated with MIR503HG and negatively correlated with miR-503-5p.Overexpressed MIR503HG/miR-503-5p did not affect the migration and angiogenesis of HRMECs cultured separately,but significantly affected migration and angiogenesis of HRMEC in HPF and HRMEC co-culture models.Western blotting revealed that MIR503HG/miR-503-5p overexpression significantly increased LMW FGF2 expression in HPF.CONCLUSION:MIR503HG/miR-503-5p inhibits HRMEC migration and angiogenic function by interfering with the interaction between HPF and endothelial cells via reducing LMW FGF2 in HPF.
文摘活动断裂带能够向大气释放一定量的温室气体及有害气体(如CO_2,Rn,Hg等),对大气环境产生一定的影响.于2012年5月和2013年5月对首都圈西北部9条活动断裂带CO_2、Rn和Hg的脱气浓度及通量进行了测量,并计算了CO_2和Hg脱气对大气的年贡献量.结果表明:(1)活动断裂带土壤气中CO_2、Rn和Hg浓度的平均值分别为0.58%、8.88 k Bq·m-3和6.44 ng·m-3,其浓度的最大值分别为9.47%、79.1 k Bq·m-3和105 ng·m-3;土壤气浓度整体具有从西向东,自南向北增大的趋势.断裂带CO_2、Rn和Hg通量的平均值分别为55.7 g·m-2·d-1、55.87 m Bq·m-2·s-1和6.78 ng·m-2·h-1,其通量的最大值分别为274.29 g·m-2·d-1、230.22 m Bq·m-2·s-1和74.33 ng·m-2·h-1.(2)京西北活动断裂带多数测区土壤气CO_2浓度无窒息风险;部分测区土壤气Hg浓度大于38 ng·m-3,属中度污染区;首都圈西北部9条活动断裂带通过脱气作用对大气CO_2的年贡献量为2 Mt,对大气Hg的年贡献量为5.3 kg;活动断裂带部分测点氡浓度较高,其附近建筑物应采取综合防氡措施.
文摘This study was performed to estimate the emission of non-CO 2 greenhouse gases(GHGs) from biomass burning at a large fire area.The extended methodology adopted the IPCC Guidelines(2003) equation for use on data from the Samcheok forest fire gathered using 30 m resolution Landsat TM satellite imagery,digital forest type maps,and growing stock information per hectare by forest type in 1999.Normalized burn ratio(NBR) technique was employed to analyze the area and severity of the Samcheok forest fire that occurred in 2000.The differences between NBR from pre-and post-fire datasets are examined to determine the extent and degree of change detected from burning.The results of burn severity analysis by dNBR of the Samcheok forest fire area revealed that a total of 16,200 ha of forest were burned.The proportion of the area characterized by a 'Low' burn severity(dNBR below 152) was 35%,with 'Moderate'(dNBR 153-190) and 'High'(dNBR 191-255) areas were at 33% and 32%,respectively.The combustion efficiency for burn severity was calculated as 0.43 for crown fire where burn severity was 'High',as 0.40 for 'Moderate' severity,and 0.15 for 'Low' severity surface fire.The emission factors for estimating non-CO 2 GHGs were separately applied to CO 130,CH 4 9,NO x 0.7 and N 2 O 0.11.Non-CO 2 GHGs emissions from biomass burning in the Samcheok forest fire area were estimated to be CO 44.100,CH 4 3.053,NO x 0.238 and N 2 O 0.038 Gg.
文摘芝麻是八大类食物过敏原之一,快速准确识别芝麻过敏原对预防其过敏有重要意义。核酸适配体可以高效识别靶标过敏原,在过敏原检测中有良好的应用前景。为了获得芝麻主要过敏原Ses i 2的特异性核酸适体,本研究以Ses i 2为靶标,通过磁珠筛选法(磁珠-SELEX)开展10轮筛选,经由高通量测序获得6条候补序列(S1~S6),并进行家族性、同源性分析及二级结构预测。结果表明,6条候选核酸适体的重复率可达46.38%,其自由能在-9.02到-2.47 kcal·moL^(-1)之间,根据自由能能量稳定原则,S1和S5吉布斯自由能最低最稳定,分别为-6.70和-9.02 kcal·moL^(-1)。利用ELISA试验进行亲和力测试,结果表明核酸适体S1和S2的亲和能力较强,S1:KD=67.02 nmol·L^(-1),R2=0.925 8,S2:KD=97.65 nmol·L^(-1),R2=0.795 1。核酸适体S1与过敏原Ses i 2的结合力和其他过敏原蛋白相比有显著差异,可视为具有特异性。本研究最终获得一条兼具良好亲和力和特异性的核酸适体S1,为芝麻过敏原快速检测提供了技术支撑。
