Cotton rose(Hibiscus mutabilis)is a well-known ornamental plant that produces large flowers of vibrant colors.However,metabolites in H.mutabilis flowers with vibrant color have not been fully understood.By performing ...Cotton rose(Hibiscus mutabilis)is a well-known ornamental plant that produces large flowers of vibrant colors.However,metabolites in H.mutabilis flowers with vibrant color have not been fully understood.By performing a combined analysis of metabolomics and transcriptomics data,we here explored mechanisms for the production of primary active compounds in this plant.Multivariate statistics unveiled differences in flavonoid metabolism between white and pink flowers,with pink flowers exhibiting a greater flavonoid abundance.The white-to-pink transition of cotton rose flowers may be attributed to pelargonidin-3-O-glucoside formation.On examining the expression of genes related to the structure of flavonoids,pink flowers were found to have a higher number of upregulated genes than white flowers,which resulted in higher flavonoid accumulation in the pink flowers.These results underscore the potential applications and development value of cotton rose flowers.Our study provides relevant insights into the regulation of key active components and the theoretical basis for the efficient utilization of cotton rose flowers.展开更多
Objective:To provide in vitro evidence for antidiabetic activity through potential inhibition of α-glucosidase enzyme,glucose diffusion and enhancement in the wound healing using methanolic extract and fractions from...Objective:To provide in vitro evidence for antidiabetic activity through potential inhibition of α-glucosidase enzyme,glucose diffusion and enhancement in the wound healing using methanolic extract and fractions from Hibiscus sabdariffa Linn.fruit.Methods:The inhibitory action of methanolic extract and fractions of such fruit on aglucosidase enzyme and glucose movement through in vitro assay assessment was reported.Their activities on wound healing were tested using the scratch assay.Results:Ethyl acetate fraction at 50 mg/m L concentration exhibited significant aglucosidase inhibition(95.79 mg/m L) with P < 0.05.At the same concentration,the methanolic extract as well as other fractions revealed lower α-glucosidase inhibition and higher glucose diffusion retardation across the dialysis tube than the control.Ethyl acetate and butanol fractions displayed notably higher glucose diffusion inhibitory activity of 5.21 mmol/L and 5.2 mmol/L,respectively as compared to methanolic extract and n-hexane fraction of 6.58 mmol/L and 6.49 mmol/L,respectively.Conversely,compared to other fractions the methanolic extract and ethyl acetate fraction manifested proliferative effect at the incubation time of 6 h during the wound healing study.Conclusions:It is established that methanolic extract and fractions from H.sabdariffa Linn.fruit can inhibit the α-glucosidase enzyme and glucose movement as well as influence the wound healing activity positively.展开更多
基金supported by the college students’Innovation and Entrepreneurship Training Program of Chengdu University(S202311079115,CDUCX2024599).
文摘Cotton rose(Hibiscus mutabilis)is a well-known ornamental plant that produces large flowers of vibrant colors.However,metabolites in H.mutabilis flowers with vibrant color have not been fully understood.By performing a combined analysis of metabolomics and transcriptomics data,we here explored mechanisms for the production of primary active compounds in this plant.Multivariate statistics unveiled differences in flavonoid metabolism between white and pink flowers,with pink flowers exhibiting a greater flavonoid abundance.The white-to-pink transition of cotton rose flowers may be attributed to pelargonidin-3-O-glucoside formation.On examining the expression of genes related to the structure of flavonoids,pink flowers were found to have a higher number of upregulated genes than white flowers,which resulted in higher flavonoid accumulation in the pink flowers.These results underscore the potential applications and development value of cotton rose flowers.Our study provides relevant insights into the regulation of key active components and the theoretical basis for the efficient utilization of cotton rose flowers.
基金the ministry of higher education of Iraq for financial support
文摘Objective:To provide in vitro evidence for antidiabetic activity through potential inhibition of α-glucosidase enzyme,glucose diffusion and enhancement in the wound healing using methanolic extract and fractions from Hibiscus sabdariffa Linn.fruit.Methods:The inhibitory action of methanolic extract and fractions of such fruit on aglucosidase enzyme and glucose movement through in vitro assay assessment was reported.Their activities on wound healing were tested using the scratch assay.Results:Ethyl acetate fraction at 50 mg/m L concentration exhibited significant aglucosidase inhibition(95.79 mg/m L) with P < 0.05.At the same concentration,the methanolic extract as well as other fractions revealed lower α-glucosidase inhibition and higher glucose diffusion retardation across the dialysis tube than the control.Ethyl acetate and butanol fractions displayed notably higher glucose diffusion inhibitory activity of 5.21 mmol/L and 5.2 mmol/L,respectively as compared to methanolic extract and n-hexane fraction of 6.58 mmol/L and 6.49 mmol/L,respectively.Conversely,compared to other fractions the methanolic extract and ethyl acetate fraction manifested proliferative effect at the incubation time of 6 h during the wound healing study.Conclusions:It is established that methanolic extract and fractions from H.sabdariffa Linn.fruit can inhibit the α-glucosidase enzyme and glucose movement as well as influence the wound healing activity positively.
