Establishment of a highly efficient regeneration system for the mature embryo of wheat will provide a convenient tool for wheat tissue culture and transformation, thereby facilitating the transformation of foreign gen...Establishment of a highly efficient regeneration system for the mature embryo of wheat will provide a convenient tool for wheat tissue culture and transformation, thereby facilitating the transformation of foreign genes into wheat. By using the mature embryos derived from 20 different wheat lines including Shi 4185, Yumai 66, Lunxuan 987, CB037, Yangmai 6, Xinchun 9, Bobwhite, Han 6172, Zheng 9023, Jimai 20, Ningchun 4, and Jing 411, the effects of some factors including inoculation methods, initiating culture media, organic additives, antioxidants, and auxins on the regeneration from the explants were evaluated. The results indicated that the scraping embryo culture was better than the whole embryo culture, the Aa medium was better than the SD2 medium and dicamba was better than 2,4-D in increasing the regeneration frequency. An Adi medium was established in this study by adding silver nitrate, cysteine, ascorbic acid, dicamba, glutamine into the Aa medium at the concentration of 4,40, 100, 2, and 5 mg L^-1, respectively. By using the Adi medium and the scraping technique, the regeneration frequencies of the mature embryos of CB037, Lunxuan 987, Hart 6172, Yangmai 6, Bobwhite, Zheng 9023, Shi 4 185, and Jimai 20 became 85.6, 60,1, 46.0, 42.1,42.0, 34.0, 33.0, and 32.0%, respectively, which were about 5-8 times higher than that obtained from the conventional culture mediums and techniques. This novel regeneration system could be helpful in wheat transformation.展开更多
Granular bed filter(GBF)has become one of the current research hot topics due to its excellent performance in removing fine particles.In this paper,a three-dimensional fixed bed GBF filtration model was established an...Granular bed filter(GBF)has become one of the current research hot topics due to its excellent performance in removing fine particles.In this paper,a three-dimensional fixed bed GBF filtration model was established and its accuracy was verified.Then,the GBF filtration performance at high temperature were studied.The results demonstrate that elevating the temperature diminishes the filtration efficiency,albeit to a limited extent.The increasing of inlet gas velocity can significantly improve pressure drop for GBF and the filtration efficiency for fine particles of sizes larger than 5μm.As the diameter of stacked granular particle diameter grows,the filtration efficiency and pressure drop drops.The density of fine particles almost does not affect the filtration efficiency for fine particles of 1∼7μm,but a higher density leads to a higher filtration efficiency for fine particles of sizes bigger than 9μm.Additionally,as the fine particles size increases,the change of the filtration efficiency roughly goes through three stages:Stage 1:the filtration efficiency is basically unchanged;Stage 2:the filtration efficiency increases rapidly;Stage 3:the filtration efficiency increases steadily,but the rate of increase slows down.With the increase of the fine particles Stokes number,the filtration efficiency of GBF will pass through two phases of stabilization and rapid increase.展开更多
A novel bonding method using silicate gel as bonding medium is developed.High reflective SiO 2/Si mirrors deposited on silicon substrates by e-beam deposition are bonded to the active layers at a low temperature of ...A novel bonding method using silicate gel as bonding medium is developed.High reflective SiO 2/Si mirrors deposited on silicon substrates by e-beam deposition are bonded to the active layers at a low temperature of 350℃ without any special treatment on bonding surfaces.The reflectivities of the mirrors can be as high as 99 9%.A Si-based narrow band response InGaAs photodetector is successfully fabricated,with a quantum efficiency of 22 6% at the peak wavelength of 1 54μm,and a full width at half maximum of about 27nm.This method has a great potential for industry processes.展开更多
基金funded by the National Natural Science Foundation of China (30971776)the National Transgenic Organism Research Program of China(2008ZX08010-004)
文摘Establishment of a highly efficient regeneration system for the mature embryo of wheat will provide a convenient tool for wheat tissue culture and transformation, thereby facilitating the transformation of foreign genes into wheat. By using the mature embryos derived from 20 different wheat lines including Shi 4185, Yumai 66, Lunxuan 987, CB037, Yangmai 6, Xinchun 9, Bobwhite, Han 6172, Zheng 9023, Jimai 20, Ningchun 4, and Jing 411, the effects of some factors including inoculation methods, initiating culture media, organic additives, antioxidants, and auxins on the regeneration from the explants were evaluated. The results indicated that the scraping embryo culture was better than the whole embryo culture, the Aa medium was better than the SD2 medium and dicamba was better than 2,4-D in increasing the regeneration frequency. An Adi medium was established in this study by adding silver nitrate, cysteine, ascorbic acid, dicamba, glutamine into the Aa medium at the concentration of 4,40, 100, 2, and 5 mg L^-1, respectively. By using the Adi medium and the scraping technique, the regeneration frequencies of the mature embryos of CB037, Lunxuan 987, Hart 6172, Yangmai 6, Bobwhite, Zheng 9023, Shi 4 185, and Jimai 20 became 85.6, 60,1, 46.0, 42.1,42.0, 34.0, 33.0, and 32.0%, respectively, which were about 5-8 times higher than that obtained from the conventional culture mediums and techniques. This novel regeneration system could be helpful in wheat transformation.
基金support provided by the National Natural Science Foundation of China(grant No.51878356).
文摘Granular bed filter(GBF)has become one of the current research hot topics due to its excellent performance in removing fine particles.In this paper,a three-dimensional fixed bed GBF filtration model was established and its accuracy was verified.Then,the GBF filtration performance at high temperature were studied.The results demonstrate that elevating the temperature diminishes the filtration efficiency,albeit to a limited extent.The increasing of inlet gas velocity can significantly improve pressure drop for GBF and the filtration efficiency for fine particles of sizes larger than 5μm.As the diameter of stacked granular particle diameter grows,the filtration efficiency and pressure drop drops.The density of fine particles almost does not affect the filtration efficiency for fine particles of 1∼7μm,but a higher density leads to a higher filtration efficiency for fine particles of sizes bigger than 9μm.Additionally,as the fine particles size increases,the change of the filtration efficiency roughly goes through three stages:Stage 1:the filtration efficiency is basically unchanged;Stage 2:the filtration efficiency increases rapidly;Stage 3:the filtration efficiency increases steadily,but the rate of increase slows down.With the increase of the fine particles Stokes number,the filtration efficiency of GBF will pass through two phases of stabilization and rapid increase.
文摘A novel bonding method using silicate gel as bonding medium is developed.High reflective SiO 2/Si mirrors deposited on silicon substrates by e-beam deposition are bonded to the active layers at a low temperature of 350℃ without any special treatment on bonding surfaces.The reflectivities of the mirrors can be as high as 99 9%.A Si-based narrow band response InGaAs photodetector is successfully fabricated,with a quantum efficiency of 22 6% at the peak wavelength of 1 54μm,and a full width at half maximum of about 27nm.This method has a great potential for industry processes.
