Objective To obtain precise data on the changes in the levels of 29 cytokines in mice after high or low linear energy transfer(LET)irradiation and to develop an accurate model of radiation exposure based on the cytoki...Objective To obtain precise data on the changes in the levels of 29 cytokines in mice after high or low linear energy transfer(LET)irradiation and to develop an accurate model of radiation exposure based on the cytokine levels after irradiation.Methods Plasma samples harvested from mice at different time points after carbon-ion or X-ray irradiation were analyzed using meso-scale discovery(MSD),a high-throughput and sensitive electrochemiluminescence measurement technique.Dose estimation equations were set up using multiple linear regression analysis.Results The relative levels of IL-6 at 1 h,IL-5 and IL-6 at 24 h,and IL-5,IL-6 and IL-15 at 7 d after irradiation with two intensities increased dose-dependently.The minimum measured levels of IL-5,IL-6 and IL-15 were up to 4.0076 pg/mL,16.4538 pg/mL and 0.4150 pg/mL,respectively.In addition,dose estimation models were established and verified.Conclusions The MSD assay can provide more accurate data regarding the changes in the levels of the cytokines IL-5,IL-6 and IL-15.These cytokines could meet the essential criteria for radiosensitive biomarkers and can be used as radiation indicators.Our prediction models can conveniently and accurately estimate the exposure dose in irradiated organism.展开更多
This study investigated the impact of simulated microgravity on acute injury induced by low doses of carbon ions in male reproductive organs of mice,and determined alterations in spermatogenic function and expression ...This study investigated the impact of simulated microgravity on acute injury induced by low doses of carbon ions in male reproductive organs of mice,and determined alterations in spermatogenic function and expression levels of apoptotic factors in mice following exposure to acute irradiation after 7 days of simulated microgravity.The results demonstrated that significant reductions in spermatozoa,primary spermatocytes and spermatogonia,and increased globular cells in seminiferous tubule and pro-apoptotic proteins were observed in the group exposed to over0.4 Gy irradiation.Collectively,the data suggest that lesions inflicted by simulated microgravity are not markedly modified by lower doses of irradiation(0.2 Gy)in mouse testis compared to the control group.However,testicular impairments were markedly evident in the group exposed to higher doses of carbon ions plus simulated microgravity,which may be due at least in part to elevated apoptosis initiated by the mitochondrial apoptosis pathway in germ cells.展开更多
目的：采用Meta分析方法评价let-7低表达在肺癌预后中的作用及高迁移率旋蛋白A2（HMGA2）表达与肺癌关系的临床意义。方法：计算机检索PubMed、Web of Science、Medline、Cochrane Library、Google、Scholar、CNKI、CBM、万方和维普等...目的：采用Meta分析方法评价let-7低表达在肺癌预后中的作用及高迁移率旋蛋白A2（HMGA2）表达与肺癌关系的临床意义。方法：计算机检索PubMed、Web of Science、Medline、Cochrane Library、Google、Scholar、CNKI、CBM、万方和维普等数据库,收集关于let-7低表达与肺癌预后及HMGA2与肺癌发生发展的关联性研究,采用StataSE 12.0软件进行Meta分析。结果：共纳入10篇关于let-7低表达与肺癌预后关系的文献用于Meta分析,均为非小细胞肺癌患者。let-7低表达预示着肺癌患者较差的总生存率,风险比（HR）的合并值为1.55（95%CI：1.16～2.09,P〈0.05）。共纳入9篇关于HMGA2表达与非小细胞肺癌发生发展关系的病例对照研究用于Meta分析,肺癌组织中HMGA2表达阳性率明显高于癌旁正常组织（OR=5.92,95%CI：3.96～8.85,P=0.000）。HMGA2在肺癌组织的表达与肺癌TNM分期、有无淋巴结转移有明显关联（OR=0.26,95%CI：0.16～0.41,P〈0.05;OR=0.29,95%CI：0.12～0.71,P〈0.05）。结论：肺癌患者中let-7低表达是肺癌患者预后的危险因素。HMGA2表达阳性率越高,肺癌发生的概率就越高,越具有侵袭性。展开更多
空间高能质子和重离子是导致元器件发生单粒子效应的根本原因,为准确评估元器件在轨遭遇的单粒子效应风险,必须清楚高能质子、重离子与器件材料发生核反应的物理过程及生成的次级重离子LET(Line Energy Transfer)分布规律。针对典型CMO...空间高能质子和重离子是导致元器件发生单粒子效应的根本原因,为准确评估元器件在轨遭遇的单粒子效应风险,必须清楚高能质子、重离子与器件材料发生核反应的物理过程及生成的次级重离子LET(Line Energy Transfer)分布规律。针对典型CMOS工艺器件模拟计算了不同能量质子和氦核粒子在器件灵敏单元内产生的反冲核、平均能量及线性能量转移值,并分析了半导体器件金属布线层中重金属对次级重离子LET分布的影响规律。计算结果表明:高能粒子与器件相互作用后产生大量次级重离子,且高能质子作用后产生的次级粒子的LET值主要分布为0~25MeV·cm^2/mg;高能氦核粒子作用后产生的次级粒子的LET值主要分布为0~35 MeV·cm^2/mg;有重金属钨(W)存在时能提高次级粒子的LET值,增加了半导体器件发生单粒子效应的概率,该研究结果可为元器件单粒子效应风险分析、航天器抗单粒子效应指标确定提供重要依据。展开更多
基金supported by the National Natural Science Foundation of China[11635013,11705248,U1832101]National Key Research and Development Program of China[2017YFC0108605]the Science and Technology Research Project of Gansu Province[No.145RTSA012 and 17JR5RA307]。
文摘Objective To obtain precise data on the changes in the levels of 29 cytokines in mice after high or low linear energy transfer(LET)irradiation and to develop an accurate model of radiation exposure based on the cytokine levels after irradiation.Methods Plasma samples harvested from mice at different time points after carbon-ion or X-ray irradiation were analyzed using meso-scale discovery(MSD),a high-throughput and sensitive electrochemiluminescence measurement technique.Dose estimation equations were set up using multiple linear regression analysis.Results The relative levels of IL-6 at 1 h,IL-5 and IL-6 at 24 h,and IL-5,IL-6 and IL-15 at 7 d after irradiation with two intensities increased dose-dependently.The minimum measured levels of IL-5,IL-6 and IL-15 were up to 4.0076 pg/mL,16.4538 pg/mL and 0.4150 pg/mL,respectively.In addition,dose estimation models were established and verified.Conclusions The MSD assay can provide more accurate data regarding the changes in the levels of the cytokines IL-5,IL-6 and IL-15.These cytokines could meet the essential criteria for radiosensitive biomarkers and can be used as radiation indicators.Our prediction models can conveniently and accurately estimate the exposure dose in irradiated organism.
基金Supported by grants from the National Basic Research Program of China(No.2010CB834202)Knowledge Innovation Projection of Chinese Academy of Science(No.KJCX2-YW-L08)The National Natural Science Foundation of China(No.10835011,No.10675151,No.41161058,No.31060065 and No.10805064)
文摘This study investigated the impact of simulated microgravity on acute injury induced by low doses of carbon ions in male reproductive organs of mice,and determined alterations in spermatogenic function and expression levels of apoptotic factors in mice following exposure to acute irradiation after 7 days of simulated microgravity.The results demonstrated that significant reductions in spermatozoa,primary spermatocytes and spermatogonia,and increased globular cells in seminiferous tubule and pro-apoptotic proteins were observed in the group exposed to over0.4 Gy irradiation.Collectively,the data suggest that lesions inflicted by simulated microgravity are not markedly modified by lower doses of irradiation(0.2 Gy)in mouse testis compared to the control group.However,testicular impairments were markedly evident in the group exposed to higher doses of carbon ions plus simulated microgravity,which may be due at least in part to elevated apoptosis initiated by the mitochondrial apoptosis pathway in germ cells.
文摘目的：采用Meta分析方法评价let-7低表达在肺癌预后中的作用及高迁移率旋蛋白A2（HMGA2）表达与肺癌关系的临床意义。方法：计算机检索PubMed、Web of Science、Medline、Cochrane Library、Google、Scholar、CNKI、CBM、万方和维普等数据库,收集关于let-7低表达与肺癌预后及HMGA2与肺癌发生发展的关联性研究,采用StataSE 12.0软件进行Meta分析。结果：共纳入10篇关于let-7低表达与肺癌预后关系的文献用于Meta分析,均为非小细胞肺癌患者。let-7低表达预示着肺癌患者较差的总生存率,风险比（HR）的合并值为1.55（95%CI：1.16～2.09,P〈0.05）。共纳入9篇关于HMGA2表达与非小细胞肺癌发生发展关系的病例对照研究用于Meta分析,肺癌组织中HMGA2表达阳性率明显高于癌旁正常组织（OR=5.92,95%CI：3.96～8.85,P=0.000）。HMGA2在肺癌组织的表达与肺癌TNM分期、有无淋巴结转移有明显关联（OR=0.26,95%CI：0.16～0.41,P〈0.05;OR=0.29,95%CI：0.12～0.71,P〈0.05）。结论：肺癌患者中let-7低表达是肺癌患者预后的危险因素。HMGA2表达阳性率越高,肺癌发生的概率就越高,越具有侵袭性。
文摘空间高能质子和重离子是导致元器件发生单粒子效应的根本原因,为准确评估元器件在轨遭遇的单粒子效应风险,必须清楚高能质子、重离子与器件材料发生核反应的物理过程及生成的次级重离子LET(Line Energy Transfer)分布规律。针对典型CMOS工艺器件模拟计算了不同能量质子和氦核粒子在器件灵敏单元内产生的反冲核、平均能量及线性能量转移值,并分析了半导体器件金属布线层中重金属对次级重离子LET分布的影响规律。计算结果表明:高能粒子与器件相互作用后产生大量次级重离子,且高能质子作用后产生的次级粒子的LET值主要分布为0~25MeV·cm^2/mg;高能氦核粒子作用后产生的次级粒子的LET值主要分布为0~35 MeV·cm^2/mg;有重金属钨(W)存在时能提高次级粒子的LET值,增加了半导体器件发生单粒子效应的概率,该研究结果可为元器件单粒子效应风险分析、航天器抗单粒子效应指标确定提供重要依据。
