On the basis of the data obtained from the comprehensive Kuroshio surveys in 1987-1988,this paper analyses the oceanographic characteristics in the area (125°-130° E,27°-31° N) of the continental s...On the basis of the data obtained from the comprehensive Kuroshio surveys in 1987-1988,this paper analyses the oceanographic characteristics in the area (125°-130° E,27°-31° N) of the continental shelf edge of the East China Sea (E. C. S. ) and its adjacent waters and discusses the effects of the Kuroshio front,thermocline and upwelling of the Kuroshio subsurface water on the distribution of standing stock of phytoplankton (chlorophyll-a). The distribution of high content of chlorophylly-a has been detected at 20-50 in depth in the water body on the left side of the Kuroshio front in the continental shelf edge waters of the E. C. S. The high content of chlorophyll-a spreads from the shelf area to the Kuroshio area in the form of a tongue and connects with the maximum layer of subsurface chlorophyll-a of the Kuroshio and pelagic sea. The author considers that the formation of the distribution of high content chlorophyll-a in this area results from the bottom topography and oceanic environment and there are close correlations between the high content of chlorophyll-a and the light-nutrient environment.展开更多
Fluorescence lifetime imaging(FLIM)is increasingly used to read out cellular autofluorescenceoriginating from the coenzyme NADH in the context of investigating cell metabolic state.Wepresent here an automated multiwel...Fluorescence lifetime imaging(FLIM)is increasingly used to read out cellular autofluorescenceoriginating from the coenzyme NADH in the context of investigating cell metabolic state.Wepresent here an automated multiwell plate reading FLIM microscope optimized for UV illumi-nation with the goal of extending high content fluorescence lifetime asays to readouts ofmetabolism,We demonstrate its application to automated cellular autofluorescence lifetime imaging and discuss the key practical issues associated with its implementation.In particular,weillustrate its capability to read out the NADH-lifetime response of cells to metabolic modulators,thereby illustrating the potential of the instrument for cytotoxicity studies,assays for drugdiscovery and stratified medicine.展开更多
文摘On the basis of the data obtained from the comprehensive Kuroshio surveys in 1987-1988,this paper analyses the oceanographic characteristics in the area (125°-130° E,27°-31° N) of the continental shelf edge of the East China Sea (E. C. S. ) and its adjacent waters and discusses the effects of the Kuroshio front,thermocline and upwelling of the Kuroshio subsurface water on the distribution of standing stock of phytoplankton (chlorophyll-a). The distribution of high content of chlorophylly-a has been detected at 20-50 in depth in the water body on the left side of the Kuroshio front in the continental shelf edge waters of the E. C. S. The high content of chlorophyll-a spreads from the shelf area to the Kuroshio area in the form of a tongue and connects with the maximum layer of subsurface chlorophyll-a of the Kuroshio and pelagic sea. The author considers that the formation of the distribution of high content chlorophyll-a in this area results from the bottom topography and oceanic environment and there are close correlations between the high content of chlorophyll-a and the light-nutrient environment.
基金funding from the UK Biotechnology and Biological Sciences Research Council(BBSRCBB/H00713X/1)the UK Engineering and PhysicalSciences Research Council(EPSRC EP/IO2770X/1)the UK Technology Strategy Board Technology Award(CHBT/007/00030,EP/C54269X,inpartnership with Astra Zeneca,GE Healthcare,GSK,Kentech Instruments Ltd).
文摘Fluorescence lifetime imaging(FLIM)is increasingly used to read out cellular autofluorescenceoriginating from the coenzyme NADH in the context of investigating cell metabolic state.Wepresent here an automated multiwell plate reading FLIM microscope optimized for UV illumi-nation with the goal of extending high content fluorescence lifetime asays to readouts ofmetabolism,We demonstrate its application to automated cellular autofluorescence lifetime imaging and discuss the key practical issues associated with its implementation.In particular,weillustrate its capability to read out the NADH-lifetime response of cells to metabolic modulators,thereby illustrating the potential of the instrument for cytotoxicity studies,assays for drugdiscovery and stratified medicine.
