Optimization of Inactivation Conditions of High Hydrostatic Pressure Using Response Surface Methodology

Response surface methodology (RSM) was employed in the present work and a second orderquadratic equation for high hydrostatic pressure (HHP) inactivation was built. Theadequacy of the model equation for predicting the optimum response values was verifiedeffectively by the validation data. Effects of temperature, pressure, and pressureholding time on HHP inactivation of Escherichia coli ATCC 8739 were explored. Byanalyzing the response surface plots and their corresponding contour plots as well assolving the quadratic equation, the optimum process parameters for inactivation E. coliof six log cycles were obtained as: temperature 32.2℃, pressure 346.4 MPa, and pressureholding time 12.6min.

超高压杀菌条件的响应曲面法优化研究

采用响应曲面法(responsesurfacemethodology,RSM)建立了超高压杀灭大肠杆菌的二次多项数学模型,验证了模型的有效性,并考察温度、压力和保压时间对超高压杀灭大肠杆菌的影响,优化出杀灭6个数量级大肠杆菌ATCC8739的工艺参数温度为32.2,压力346.4MPa,保压时间12.6min。