A new rapid method for the analysis of chromate ion in water by using high performance capillary electrophoresis is described. The detection limit is 1.0 mu g/ml by using 100 m mol/l Tris-HCl buffer containing 2 m mol...A new rapid method for the analysis of chromate ion in water by using high performance capillary electrophoresis is described. The detection limit is 1.0 mu g/ml by using 100 m mol/l Tris-HCl buffer containing 2 m mol/l 1,6-hexanediamine which was used as an electroosmosis modifier to improve the separation and detection sensitivity.展开更多
Capillary electrochromatography(CEC)plays a significant role in chiral separation via the double separation principle,partition coefficient difference between the two phases,and electroosmotic flow-driven separation.G...Capillary electrochromatography(CEC)plays a significant role in chiral separation via the double separation principle,partition coefficient difference between the two phases,and electroosmotic flow-driven separation.Given the distinct properties of the inner wall stationary phase(SP),the separation ability of each SP differs from one another.Particularly,it provides large room for promising applications of open tubular capillary electrochromatography(OT-CEC).We divided the OT-CEC SPs developed over the past four years into six types:ionic liquids,nanoparticle materials,microporous materials,biomaterials,non-nanopolymers,and others,to mainly introduce their characteristics in chiral drug separation.There also added a few classic SPs that occurred within ten years as supplements to enrich the features of each SP.Additionally,we discuss their applications in metabolomics,food,cosmetics,environment,and biology as analytes in addition to chiral drugs.OT-CEC plays an increasingly significant role in chiral separation and may promote the development of capillary electrophoresis(CE)combined with other instruments in recent years,such as CE with mass spectrometry(CE/MS)and CE with ultraviolet light detector(CE/UV).展开更多
Sulfobutylether－β－cyclodextrin（SBE－β－CD）was used as a chiral selector tor separatingten chlral drugs with resolution 1．2 by capillary zone electrophoresls（CZE）， The backgroundelectrolylc solution compris...Sulfobutylether－β－cyclodextrin（SBE－β－CD）was used as a chiral selector tor separatingten chlral drugs with resolution 1．2 by capillary zone electrophoresls（CZE）， The backgroundelectrolylc solution comprised of 120 mmol／L Britton－Robinson buffer（BRB） containing1 ～10mmol／L SBE－β－CD with the pH value adjusted from 5．0－6．8． Five of the drugs were better resolvedthan those previously reported with neutral CDs．展开更多
A method using carboxymethyl-b-cyclodextrin (CM-b-CD) as selector for chiral separation of amino acids by capillary electrophoresis and laser-induced fluorescence detection was studied. Resolution was better than tha...A method using carboxymethyl-b-cyclodextrin (CM-b-CD) as selector for chiral separation of amino acids by capillary electrophoresis and laser-induced fluorescence detection was studied. Resolution was better than that obtained by b-CD or HP-b-CD.展开更多
β-Cyclodextrin phosphate (β-CD-phosphate) was used as a selector for separating chiral drugs by capillary electrophoresis (CE). A solution comprising of 120 mmol/L Britton Robinson buffer (BRB) containing 10 mmol/L ...β-Cyclodextrin phosphate (β-CD-phosphate) was used as a selector for separating chiral drugs by capillary electrophoresis (CE). A solution comprising of 120 mmol/L Britton Robinson buffer (BRB) containing 10 mmol/L 0-CD phosphate with the pH adjusted to 7.0 was used as the background electrolyte (BGE), and a small amount of analyte was injected (600v/1s). Triethylamine, diethylamine, triethanolamine, diethanolamine, Tris added as modifier were compared. Isoprenaline, methoxamine, oxprenolol, practolol were successfully resolved.展开更多
A successful chiral separation of N-benzoyl phenylalanine methyl ester has been achieved by nonaqueous capillary electrophoresis (NACE) using P-CD as chiral selector in formamide (FA). Some experimental parameters inf...A successful chiral separation of N-benzoyl phenylalanine methyl ester has been achieved by nonaqueous capillary electrophoresis (NACE) using P-CD as chiral selector in formamide (FA). Some experimental parameters influencing the chiral separation such as concentration of P-CD, ionic strength and apparent pH (pH*) are discussed.展开更多
Cyclodextrin-modified CZE was applied to the chiral separation of two basic drugs, i.e., esmolol and terazosin. Selector screening and concentration optimization experiments were performed. Resolution 3.1 for esmolol,...Cyclodextrin-modified CZE was applied to the chiral separation of two basic drugs, i.e., esmolol and terazosin. Selector screening and concentration optimization experiments were performed. Resolution 3.1 for esmolol, 1.2 for terazosin were achieved when using 60 mmol/L gamma-CD and 60 mmoL/L DM-P-CD, respectively, in 50 mmol/L pH 2.5 sodium phosphate buffer.展开更多
Development of effective chromatographic or electrophoretic separation involves judicious deciding of selection of optimal experimental conditions that can provide an adequate resolution at a reasonable run time for t...Development of effective chromatographic or electrophoretic separation involves judicious deciding of selection of optimal experimental conditions that can provide an adequate resolution at a reasonable run time for the separation of interested components. Box-Behnken factorial design was effectively applied for the separation optimization of eight structurally related sulfonamides using capillary zone electrophorosis and reverse high performance liquid chromatography. Optimum values for volume ratio of THF to H2O in eluent, column temperature and flow rate of eluent are found as 12 to 88, 35℃ and 1.0 mL/min, respectively. Box-Behnken modified optimization model is extended to separation by capillary electrophoresis (CE). While using CE, a satisfactory separation is achieved with a minimum resolution larger than 1.0 for a separation time less than 10 min.展开更多
Separation of fluoxetine enantiomers on five chiral stationary phases （chiralcel OD-H, chiralcel OJ-H, chiralpak AD-H, cyclobond 1 2000 DM and kromasil CHI-TBB） was investigated. The optimal mobile phase composition...Separation of fluoxetine enantiomers on five chiral stationary phases （chiralcel OD-H, chiralcel OJ-H, chiralpak AD-H, cyclobond 1 2000 DM and kromasil CHI-TBB） was investigated. The optimal mobile phase compositions of fluoxetine separation on each column were hexane/isopropanol/diethyl amine （98/2/0.2, v/v/v）, hexane/isopropanol/diethyl amine （99/1/0. l, v/v/v）, hexane/isopropanol/diethyl amine （98/2/0.2, v/v/v）, methanol/0.2% triethylamine acetic acid （TEAA） （25/75, v/v; pH 3.8） and hexane/isopropanol/diethyl amine （98/2/0.2, v/v/v）, respectively. Experimental results demonstrated that baseline separation （Rs〉1.5） of fluoxetine enantiomers was obtained on chiralcel OD-H, chiralpak AD-H, and cyclobond I 2000 DM while the best separation was obtained on the last one. The eluate orders of fluoxetine enantiomers on the columns were determined. The first eluate by chiralcel OJ-H and kromasil CHI-TBB is the S-enantiomer, while by chiralpak AD-H and cyclobond 12000 DM is the R-enantiomer.展开更多
Four basic drugs were separated by capillary electrophoresis (CE) usingcarboxymethyl-β-yclodextrin (CM-0-CD) as chiral selector. The effects of CM-β-CDconmptration, applied voltage. operation temperature were invest...Four basic drugs were separated by capillary electrophoresis (CE) usingcarboxymethyl-β-yclodextrin (CM-0-CD) as chiral selector. The effects of CM-β-CDconmptration, applied voltage. operation temperature were investigated. Under the selectedconditions. norephedrine. ofloxacin. terbutaline and chlorpheniramine were enantiomerallybaieline-separated using H3PO4-triethanolamine buffer at PH 3 containing 10, 5.5, 2. 2mmol/LCM-β-CD respectively.展开更多
Separation and determination of metal ions based on the formation of chelate anions with 1-Nitroso-2, 7-dihydrexynaphthalene-3, 6-disulfonic acid (1N2, 7O3, 6S) was studied by rising HPCE of the nine metal ions examin...Separation and determination of metal ions based on the formation of chelate anions with 1-Nitroso-2, 7-dihydrexynaphthalene-3, 6-disulfonic acid (1N2, 7O3, 6S) was studied by rising HPCE of the nine metal ions examined, the ions that can be detected sensitively with 1-Nitroso-2, 7-dihydrexynaphthalene-3, 6-disulfonic acid were Fe2+, Co2+, Cu2+, Ni2+, Zn2+ and Pd2+. The cobalt chelate could exist in two oxidation states of cobalt. When TBA(+) were added in electrophoretic solutions, the drastic changes in elertrophoretic mobilities of chelate were observed, which was due to the ion association between chelates anions and TBA(+). The ion association constants of chelate anions with TBA(+) were determined by using the change in electrophoretic mobilities of chelates, metal ions tested were separated within 10 min using 30cm silica capillary (50 m i. d).展开更多
Ibuprofen is widely used as a non-steroidal anti-inflammatory drug and produced as racemic mixture. Its pharmacological activity resides only in S-(+)-enantiomer, and R-()-enantiomer is not only inactive but also has ...Ibuprofen is widely used as a non-steroidal anti-inflammatory drug and produced as racemic mixture. Its pharmacological activity resides only in S-(+)-enantiomer, and R-()-enantiomer is not only inactive but also has many side effects. Thus it is necessary to separate R-enantiomer from racemic ibuprofen. We studied optical separation of racemic Ibuprofen with chiral high performance liquid chromatography (HPLC). Out of three different chiral stationary phases, which were selected on the basis of structure and availability, two were found to be ef-fective. There was optimum eluent composition for each stationary phase for good resolution in optical separation. Resolution decreased with increase of eluent flow rate, but effect of injection volume on resolution was insignificant at high eluent flow rate.展开更多
Chiral separation of dansyl amino acids by capillary electrophoresis using mixed selectors of Mn(II)- L -alanine complex and β -cyclodextrin ( β -CD) was studied. Resolution was considerably superior to that obtaine...Chiral separation of dansyl amino acids by capillary electrophoresis using mixed selectors of Mn(II)- L -alanine complex and β -cyclodextrin ( β -CD) was studied. Resolution was considerably superior to that obtained by using either Mn(II)- L -alanine complex or β -CD alone. The effects of separation parameters,such as pH value of buffer solution,capillary temperature,the concentration of Mn(II)- L -alanine complex,the types of CD and ligand on the migration times and resolutions were investigated. Six different transition metal complexes, Cu(II), Zn(II),Co(II),Ni(II),Hg(II) and Cd(II)- L -alanine complexes have been employed and compared with Mn(II) complex. Differences in retention and selectivity were found. The substitution of Cu(II),Zn(II),Co(II) and Ni(II) for Mn(II) resulted in a better chiral resolution while Hg(II) and Cd(II) showed poorer resolution abilities. The chiral separation mechanism was also discussed briefly.展开更多
文摘A new rapid method for the analysis of chromate ion in water by using high performance capillary electrophoresis is described. The detection limit is 1.0 mu g/ml by using 100 m mol/l Tris-HCl buffer containing 2 m mol/l 1,6-hexanediamine which was used as an electroosmosis modifier to improve the separation and detection sensitivity.
基金This study was funded by the Project of National Natural Science Foundation of China(Grant No.:82003705)the Shanghai Science and Technology Innovation Foundation(Grant Nos.:23010500200 and 23ZR1422700).
文摘Capillary electrochromatography(CEC)plays a significant role in chiral separation via the double separation principle,partition coefficient difference between the two phases,and electroosmotic flow-driven separation.Given the distinct properties of the inner wall stationary phase(SP),the separation ability of each SP differs from one another.Particularly,it provides large room for promising applications of open tubular capillary electrochromatography(OT-CEC).We divided the OT-CEC SPs developed over the past four years into six types:ionic liquids,nanoparticle materials,microporous materials,biomaterials,non-nanopolymers,and others,to mainly introduce their characteristics in chiral drug separation.There also added a few classic SPs that occurred within ten years as supplements to enrich the features of each SP.Additionally,we discuss their applications in metabolomics,food,cosmetics,environment,and biology as analytes in addition to chiral drugs.OT-CEC plays an increasingly significant role in chiral separation and may promote the development of capillary electrophoresis(CE)combined with other instruments in recent years,such as CE with mass spectrometry(CE/MS)and CE with ultraviolet light detector(CE/UV).
文摘Sulfobutylether－β－cyclodextrin（SBE－β－CD）was used as a chiral selector tor separatingten chlral drugs with resolution 1．2 by capillary zone electrophoresls（CZE）， The backgroundelectrolylc solution comprised of 120 mmol／L Britton－Robinson buffer（BRB） containing1 ～10mmol／L SBE－β－CD with the pH value adjusted from 5．0－6．8． Five of the drugs were better resolvedthan those previously reported with neutral CDs．
文摘A method using carboxymethyl-b-cyclodextrin (CM-b-CD) as selector for chiral separation of amino acids by capillary electrophoresis and laser-induced fluorescence detection was studied. Resolution was better than that obtained by b-CD or HP-b-CD.
文摘β-Cyclodextrin phosphate (β-CD-phosphate) was used as a selector for separating chiral drugs by capillary electrophoresis (CE). A solution comprising of 120 mmol/L Britton Robinson buffer (BRB) containing 10 mmol/L 0-CD phosphate with the pH adjusted to 7.0 was used as the background electrolyte (BGE), and a small amount of analyte was injected (600v/1s). Triethylamine, diethylamine, triethanolamine, diethanolamine, Tris added as modifier were compared. Isoprenaline, methoxamine, oxprenolol, practolol were successfully resolved.
文摘A successful chiral separation of N-benzoyl phenylalanine methyl ester has been achieved by nonaqueous capillary electrophoresis (NACE) using P-CD as chiral selector in formamide (FA). Some experimental parameters influencing the chiral separation such as concentration of P-CD, ionic strength and apparent pH (pH*) are discussed.
文摘Cyclodextrin-modified CZE was applied to the chiral separation of two basic drugs, i.e., esmolol and terazosin. Selector screening and concentration optimization experiments were performed. Resolution 3.1 for esmolol, 1.2 for terazosin were achieved when using 60 mmol/L gamma-CD and 60 mmoL/L DM-P-CD, respectively, in 50 mmol/L pH 2.5 sodium phosphate buffer.
基金Project(20235010) support by the NSFC-KOSEF Scientific Cooperation ProgramProject supported by the Program for New Century Talents of University in Henan ProvinceProgram for Backbone Teacher in Henan Province, China
文摘Development of effective chromatographic or electrophoretic separation involves judicious deciding of selection of optimal experimental conditions that can provide an adequate resolution at a reasonable run time for the separation of interested components. Box-Behnken factorial design was effectively applied for the separation optimization of eight structurally related sulfonamides using capillary zone electrophorosis and reverse high performance liquid chromatography. Optimum values for volume ratio of THF to H2O in eluent, column temperature and flow rate of eluent are found as 12 to 88, 35℃ and 1.0 mL/min, respectively. Box-Behnken modified optimization model is extended to separation by capillary electrophoresis (CE). While using CE, a satisfactory separation is achieved with a minimum resolution larger than 1.0 for a separation time less than 10 min.
文摘Separation of fluoxetine enantiomers on five chiral stationary phases （chiralcel OD-H, chiralcel OJ-H, chiralpak AD-H, cyclobond 1 2000 DM and kromasil CHI-TBB） was investigated. The optimal mobile phase compositions of fluoxetine separation on each column were hexane/isopropanol/diethyl amine （98/2/0.2, v/v/v）, hexane/isopropanol/diethyl amine （99/1/0. l, v/v/v）, hexane/isopropanol/diethyl amine （98/2/0.2, v/v/v）, methanol/0.2% triethylamine acetic acid （TEAA） （25/75, v/v; pH 3.8） and hexane/isopropanol/diethyl amine （98/2/0.2, v/v/v）, respectively. Experimental results demonstrated that baseline separation （Rs〉1.5） of fluoxetine enantiomers was obtained on chiralcel OD-H, chiralpak AD-H, and cyclobond I 2000 DM while the best separation was obtained on the last one. The eluate orders of fluoxetine enantiomers on the columns were determined. The first eluate by chiralcel OJ-H and kromasil CHI-TBB is the S-enantiomer, while by chiralpak AD-H and cyclobond 12000 DM is the R-enantiomer.
文摘Four basic drugs were separated by capillary electrophoresis (CE) usingcarboxymethyl-β-yclodextrin (CM-0-CD) as chiral selector. The effects of CM-β-CDconmptration, applied voltage. operation temperature were investigated. Under the selectedconditions. norephedrine. ofloxacin. terbutaline and chlorpheniramine were enantiomerallybaieline-separated using H3PO4-triethanolamine buffer at PH 3 containing 10, 5.5, 2. 2mmol/LCM-β-CD respectively.
文摘Separation and determination of metal ions based on the formation of chelate anions with 1-Nitroso-2, 7-dihydrexynaphthalene-3, 6-disulfonic acid (1N2, 7O3, 6S) was studied by rising HPCE of the nine metal ions examined, the ions that can be detected sensitively with 1-Nitroso-2, 7-dihydrexynaphthalene-3, 6-disulfonic acid were Fe2+, Co2+, Cu2+, Ni2+, Zn2+ and Pd2+. The cobalt chelate could exist in two oxidation states of cobalt. When TBA(+) were added in electrophoretic solutions, the drastic changes in elertrophoretic mobilities of chelate were observed, which was due to the ion association between chelates anions and TBA(+). The ion association constants of chelate anions with TBA(+) were determined by using the change in electrophoretic mobilities of chelates, metal ions tested were separated within 10 min using 30cm silica capillary (50 m i. d).
文摘Ibuprofen is widely used as a non-steroidal anti-inflammatory drug and produced as racemic mixture. Its pharmacological activity resides only in S-(+)-enantiomer, and R-()-enantiomer is not only inactive but also has many side effects. Thus it is necessary to separate R-enantiomer from racemic ibuprofen. We studied optical separation of racemic Ibuprofen with chiral high performance liquid chromatography (HPLC). Out of three different chiral stationary phases, which were selected on the basis of structure and availability, two were found to be ef-fective. There was optimum eluent composition for each stationary phase for good resolution in optical separation. Resolution decreased with increase of eluent flow rate, but effect of injection volume on resolution was insignificant at high eluent flow rate.
文摘Chiral separation of dansyl amino acids by capillary electrophoresis using mixed selectors of Mn(II)- L -alanine complex and β -cyclodextrin ( β -CD) was studied. Resolution was considerably superior to that obtained by using either Mn(II)- L -alanine complex or β -CD alone. The effects of separation parameters,such as pH value of buffer solution,capillary temperature,the concentration of Mn(II)- L -alanine complex,the types of CD and ligand on the migration times and resolutions were investigated. Six different transition metal complexes, Cu(II), Zn(II),Co(II),Ni(II),Hg(II) and Cd(II)- L -alanine complexes have been employed and compared with Mn(II) complex. Differences in retention and selectivity were found. The substitution of Cu(II),Zn(II),Co(II) and Ni(II) for Mn(II) resulted in a better chiral resolution while Hg(II) and Cd(II) showed poorer resolution abilities. The chiral separation mechanism was also discussed briefly.
