Rice variety Yuexiangzhan and its mutants induced by high pressure were studied using microsatcllite markers and soluble protein content analyses. Eleven of the 88 microsatellite primer pairs showed evident polymorphi...Rice variety Yuexiangzhan and its mutants induced by high pressure were studied using microsatcllite markers and soluble protein content analyses. Eleven of the 88 microsatellite primer pairs showed evident polymorphisms repeatedly, and the polymorphic frequencies were 3.4-11.3% between the mutants and Yuexiangzhan. The polymorphic markers were randomly located on chromosomes. The more similar the plant types of the mutants like their original variety, the less polymorphic loci were detected. In addition, there was variation in the soluble protein contents among the leaves of mutants, and the contents were significantly lower than those of the original variety.展开更多
In a field experiment, rice cultivar Shanyou 63 was used as a check cultivar to investigate the changes in activity of peroxidase and contents of malondialdehyde and soluble protein after full heading stage in flag le...In a field experiment, rice cultivar Shanyou 63 was used as a check cultivar to investigate the changes in activity of peroxidase and contents of malondialdehyde and soluble protein after full heading stage in flag leaves of two super high-yielding inbred rice, including Shengtai 1 and Yuxiangyouzhan. The results showed that the peroxidase activities and soluble protein contents in the leaves of the three cultivars tended to increase gradually to peak values and thereafter descended during stage after full heading. Moreover, both the peroxidase activities and soluble protein contents of Shengtai 1 and Yuxiangyouzhan peaked at 8 days after full heading, which was compared with that of Shanyou 63 at 4 days later. Meanwhile, the malondialdehyde contents in the leaves of the three cultivars increased gradually after full heading stage. In addition, activities of peroxidase and contents of malondialdehyde and soluble protein in different cultivars were various at the same time. In general, for Shengtai 1 and Yuxiangyouzhan, the peroxidase activities and soluble protein contents were higher than those of Shanyou 63, whereas the malondialdehyde contents were lower compared with that of Shanyou 63. And these features could make the senescence of leaf become later and slower and photosynthetic product was more in Shengtai 1 and Yuxiangyouzhan than that in Shanyou 63.展开更多
During the past ten years we did and are doing 10 generations in suburbs of Beijing from 1991-2001. In the same time we are doing 10 generation of soybean breeding near Sanya on Hainan Island during the October to May...During the past ten years we did and are doing 10 generations in suburbs of Beijing from 1991-2001. In the same time we are doing 10 generation of soybean breeding near Sanya on Hainan Island during the October to May. We made cross 398 combinations and a lot of propagation of soybean elite. We select a best local popularized variey for cross with soybean variety with 1-2 best character for breeding, for example high yielding, high oil or high protein content, high resistance to nematode and others We made alternate breeding in North and on South. In the same time we test breeding materials in two conditions: soil with high fertility and low fertility. Finally, we released two soybean cultivars: Zhonghuang 12 and Zhonghuang 13,and two cultivars will released this year. We test more than 10 soybean lines in 15 provinces. We got a good line with high oil content and high protein content. And we developed several soybean lines resistant to soybean cyst nematode.展开更多
文摘Rice variety Yuexiangzhan and its mutants induced by high pressure were studied using microsatcllite markers and soluble protein content analyses. Eleven of the 88 microsatellite primer pairs showed evident polymorphisms repeatedly, and the polymorphic frequencies were 3.4-11.3% between the mutants and Yuexiangzhan. The polymorphic markers were randomly located on chromosomes. The more similar the plant types of the mutants like their original variety, the less polymorphic loci were detected. In addition, there was variation in the soluble protein contents among the leaves of mutants, and the contents were significantly lower than those of the original variety.
基金Supported by Program for Ministry of Agriculture of the People’s Republic of China
文摘In a field experiment, rice cultivar Shanyou 63 was used as a check cultivar to investigate the changes in activity of peroxidase and contents of malondialdehyde and soluble protein after full heading stage in flag leaves of two super high-yielding inbred rice, including Shengtai 1 and Yuxiangyouzhan. The results showed that the peroxidase activities and soluble protein contents in the leaves of the three cultivars tended to increase gradually to peak values and thereafter descended during stage after full heading. Moreover, both the peroxidase activities and soluble protein contents of Shengtai 1 and Yuxiangyouzhan peaked at 8 days after full heading, which was compared with that of Shanyou 63 at 4 days later. Meanwhile, the malondialdehyde contents in the leaves of the three cultivars increased gradually after full heading stage. In addition, activities of peroxidase and contents of malondialdehyde and soluble protein in different cultivars were various at the same time. In general, for Shengtai 1 and Yuxiangyouzhan, the peroxidase activities and soluble protein contents were higher than those of Shanyou 63, whereas the malondialdehyde contents were lower compared with that of Shanyou 63. And these features could make the senescence of leaf become later and slower and photosynthetic product was more in Shengtai 1 and Yuxiangyouzhan than that in Shanyou 63.
文摘During the past ten years we did and are doing 10 generations in suburbs of Beijing from 1991-2001. In the same time we are doing 10 generation of soybean breeding near Sanya on Hainan Island during the October to May. We made cross 398 combinations and a lot of propagation of soybean elite. We select a best local popularized variey for cross with soybean variety with 1-2 best character for breeding, for example high yielding, high oil or high protein content, high resistance to nematode and others We made alternate breeding in North and on South. In the same time we test breeding materials in two conditions: soil with high fertility and low fertility. Finally, we released two soybean cultivars: Zhonghuang 12 and Zhonghuang 13,and two cultivars will released this year. We test more than 10 soybean lines in 15 provinces. We got a good line with high oil content and high protein content. And we developed several soybean lines resistant to soybean cyst nematode.
