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Highly Differentiated Target Detection under Extremely Low-Light Conditions Based on Improved YOLOX Model
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作者 Haijian Shao Suqin Lei +2 位作者 Chenxu Yan Xing Deng Yunsong Qi 《Computer Modeling in Engineering & Sciences》 SCIE EI 2024年第8期1507-1537,共31页
This paper expounds upon a novel target detection methodology distinguished by its elevated discriminatory efficacy,specifically tailored for environments characterized by markedly low luminance levels.Conventional me... This paper expounds upon a novel target detection methodology distinguished by its elevated discriminatory efficacy,specifically tailored for environments characterized by markedly low luminance levels.Conventional methodologies struggle with the challenges posed by luminosity fluctuations,especially in settings characterized by diminished radiance,further exacerbated by the utilization of suboptimal imaging instrumentation.The envisioned approach mandates a departure from the conventional YOLOX model,which exhibits inadequacies in mitigating these challenges.To enhance the efficacy of this approach in low-light conditions,the dehazing algorithm undergoes refinement,effecting a discerning regulation of the transmission rate at the pixel level,reducing it to values below 0.5,thereby resulting in an augmentation of image contrast.Subsequently,the coiflet wavelet transform is employed to discern and isolate high-discriminatory attributes by dismantling low-frequency image attributes and extracting high-frequency attributes across divergent axes.The utilization of CycleGAN serves to elevate the features of low-light imagery across an array of stylistic variances.Advanced computational methodologies are then employed to amalgamate and conflate intricate attributes originating from images characterized by distinct stylistic orientations,thereby augmenting the model’s erudition potential.Empirical validation conducted on the PASCAL VOC and MS COCO 2017 datasets substantiates pronounced advancements.The refined low-light enhancement algorithm yields a discernible 5.9%augmentation in the target detection evaluation index when compared to the original imagery.Mean Average Precision(mAP)undergoes enhancements of 9.45%and 0.052%in low-light visual renditions relative to conventional YOLOX outcomes.The envisaged approach presents a myriad of advantages over prevailing benchmark methodologies in the realm of target detection within environments marked by an acute scarcity of luminosity. 展开更多
关键词 target detection extremely low-light wavelet transformation highly differentiated features YOLOX
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Analysis of the Flow Field and Impact Force in High-Pressure Water Descaling
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作者 Yue Cui Liyuan Wang +2 位作者 Jian Wu Haisheng Liu Di Wu 《Fluid Dynamics & Materials Processing》 EI 2024年第1期165-177,共13页
This study aims to improve the performances of the high-pressure water descaling technology used in steel hot rolling processes.In particular,a 2050 mm hot rolling line is considered,and the problem is investigated by... This study aims to improve the performances of the high-pressure water descaling technology used in steel hot rolling processes.In particular,a 2050 mm hot rolling line is considered,and the problem is investigated by means of a fluid–structure interaction(FSI)method by which the descaling effect produced by rolling coils with different section sizes is examined.Assuming a flat fan-shaped nozzle at the entrance of the R1R2 roughing mill,the outflow field characteristics and the velocity distribution curve on the strike line(at a target distance of 30–120 mm)are determined.It is found that the velocity in the center region of the water jet with different target distances is higher than that in the boundary region.As the target distance increases,the velocity of the water jet in the central region decreases.Through comparison with experimental results,it is shown that the simulation model can accurately predict the impact position of the high-pressure water on the impact plate,thereby providing a computational scheme that can be used to optimize the nozzle space layout and improve the slabs’descent effect for different rolling specifications. 展开更多
关键词 high pressure water descaling flow field analysis FSI target distance strike range
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Combined proportional navigation law for interception of high-speed targets 被引量:4
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作者 Yuan LI Liang YAN +2 位作者 Ji-guang ZHAO Fan LIU Tao WANG 《Defence Technology(防务技术)》 SCIE EI CAS 2014年第3期298-303,共6页
A new proportional navigation(PN) guidance law,called combined proportional navigation(CPN),is proposed.The guidance law is designed to intercept high-speed targets,which is a common case for ballistic targets.The ran... A new proportional navigation(PN) guidance law,called combined proportional navigation(CPN),is proposed.The guidance law is designed to intercept high-speed targets,which is a common case for ballistic targets.The range of target-to-interceptor speed ratio during target interception is derived when guidance laws are applied in high-speed targets interception,and the effectiveness of negative navigation ratio in the PN-based guidance law is proven analytically in some lemmas.Based on the lemmas,the lateral acceleration command of CPN is defined,and the solution to the appearance of singularity in time-varying navigation ratio is given.The simulation results show that CPN can determine headon engagement(as PN) or tail-chase engagement(as RPN) through initial path angle compared with PN and retro proportional navigation(RPN),and can adjust the value of navigation ratio for head-on engagement or tail-chase engagement.Therefore,the capture region of CPN is larger than that of other guidance laws using PN-based methods. 展开更多
关键词 COMBINED proportional NAVIGATION Proportional NAVIGATION high-SPEED target CAPTURE region INTERCEPTION
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Human mesenchymal stem cells exhibit altered mitochondrial dynamics and poor survival in high glucose microenvironment 被引量:4
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作者 Ejlal Abu-El-Rub Fatimah Almahasneh +9 位作者 Ramada R Khasawneh Ayman Alzu'bi Doaa Ghorab Rawan Almazari Huthaifa Magableh Ahmad Sanajleh Haitham Shlool Mohammad Mazari Noor S Bader Joud Al-Momani 《World Journal of Stem Cells》 SCIE 2023年第12期1093-1103,共11页
BACKGROUND Mesenchymal stem cells(MSCs)are a type of stem cells that possess relevant regenerative abilities and can be used to treat many chronic diseases.Diabetes mellitus(DM)is a frequently diagnosed chronic diseas... BACKGROUND Mesenchymal stem cells(MSCs)are a type of stem cells that possess relevant regenerative abilities and can be used to treat many chronic diseases.Diabetes mellitus(DM)is a frequently diagnosed chronic disease characterized by hyperglycemia which initiates many multisystem complications in the long-run.DM patients can benefit from MSCs transplantation to curb down the pathological consequences associated with hyperglycemia persistence and restore the function of damaged tissues.MSCs therapeutic outcomes are found to last for short period of time and ultimately these regenerative cells are eradicated and died in DM disease model.AIM To investigate the impact of high glucose or hyperglycemia on the cellular and molecular characteristics of MSCs.METHODS Human adipose tissue-derived MSCs(hAD-MSCs)were seeded in low(5.6 mmol/L of glucose)and high glucose(25 mmol/L of glucose)for 7 d.Cytotoxicity,viability,mitochondrial dynamics,and apoptosis were deplored using specific kits.Western blotting was performed to measure the protein expression of phosphatidylinositol 3-kinase(PI3K),TSC1,and mammalian target of rapamycin(mTOR)in these cells.RESULTS hAD-MSCs cultured in high glucose for 7 d demonstrated marked decrease in their viability,as shown by a significant increase in lactate dehydrogenase(P<0.01)and a significant decrease in Trypan blue(P<0.05)in these cells compared to low glucose control.Mitochondrial membrane potential,indicated by tetramethylrhodamine ethyl ester(TMRE)fluorescence intensity,and nicotinamide adenine dinucleotide(NAD+)/NADH ratio were significantly dropped(P<0.05 for TMRE and P<0.01 for NAD+/NADH)in high glucose exposed hAD-MSCs,indicating disturbed mitochondrial function.PI3K protein expression significantly decreased in high glucose culture MSCs(P<0.05 compared to low glucose)and it was coupled with significant upregulation in TSC1(P<0.05)and downregulation in mTOR protein expression(P<0.05).Mitochondrial complexes I,IV,and V were downregulated profoundly in high glucose(P<0.05 compared to low glucose).Apoptosis was induced as a result of mitochondrial impairment and explained the poor survival of MSCs in high glucose.CONCLUSION High glucose impaired the mitochondrial dynamics and regulatory proteins in hAD-MSCs ensuing their poor survival and high apoptosis rate in hyperglycemic microenvironment. 展开更多
关键词 Mesenchymal stem cells high glucose Mitochondrial dynamics Apoptosis Poor survival Phosphatidylinositol 3-kinase/mammalian target of rapamycin pathway
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Biased retro-proportional navigation law for interception of high-speed targets with angular constraint 被引量:8
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作者 Liang YAN Ji-guang ZHAO +1 位作者 Huai-rong SHEN Yuan LI 《Defence Technology(防务技术)》 SCIE EI CAS 2014年第1期60-65,共6页
A new guidance law, called biased retro proportional navigation(BRPN), is proposed. The guidance law is designed to intercept high-speed targets with angular constraint, which can be used for ballistic target intercep... A new guidance law, called biased retro proportional navigation(BRPN), is proposed. The guidance law is designed to intercept high-speed targets with angular constraint, which can be used for ballistic target interception. BRPN guidance law is defined, and the exact time-varying bias for a required impact angle is derived. Furthermore, the simulation results(trajectory, variation of navigation ratio, capture region, etc) are compared with those of biased proportional navigation(BPN), proportional navigation and retro-proportional navigation. The results show that,at the cost of a higher intercept time, BRPN demands lower terminal lateral acceleration and has larger capture region compared to BPN. 展开更多
关键词 比例导引法 角度约束 拦截 横向加速度 制导律 仿真结果 时间成本 冲击角
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Long term integration algorithm for high-dynamic targets 被引量:1
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作者 Gang Yang Haikun Luo +1 位作者 Jing Tian Siliang Wu 《Journal of Systems Engineering and Electronics》 SCIE EI CSCD 2016年第5期979-985,共7页
A new long term integration algorithm is proposed forhigh-dynamic targets, which can resolve the problems of spectrumspread, frequency walk and pseudorandom noise (PRN)code phase curvature caused by the motion of ta... A new long term integration algorithm is proposed forhigh-dynamic targets, which can resolve the problems of spectrumspread, frequency walk and pseudorandom noise (PRN)code phase curvature caused by the motion of targets. This algorithmfirst applies a keystone transform based improved discretepolynomial-phase transform (KT-IDPT) to estimate the Dopplerchirp rate. Then, based on the estimated Doppler chirp rate,dechirping and envelope translation are performed on the partialcorrelation results to correct the spectrum spread and the codephase curvature. The simulation results demonstrate that the proposedmethod has low integration loss and computational burden. 展开更多
关键词 high-dynamic target long term integration motioncompensation.
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The Status Quo and Development Trend of High-purity Gold Sputtering Targets 被引量:1
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作者 YANG Anheng XIE Hongchao ZHU Yong 《贵金属》 CAS CSCD 北大核心 2012年第A01期173-176,共4页
This article gives a brief introduction to manufacturers and markets of sputtering targets as well as the manufacturing technology thereof. Then, it analyzes the application of high-purity gold sputtering targets in t... This article gives a brief introduction to manufacturers and markets of sputtering targets as well as the manufacturing technology thereof. Then, it analyzes the application of high-purity gold sputtering targets in the fields of integrated circuit, information storage, flat panel display, etc. Based on the above, the article analyzes the processing development trend for the high-purity gold sputtering targets in aspects of ultra-high purity, manufacturing technology, analysis and testing technologies. 展开更多
关键词 high-purity gold sputtering targets status quo development trend
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Projected changes in the western North Pacific subtropical high under six global warming targets
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作者 FU Yuanhai GUO Dong 《Atmospheric and Oceanic Science Letters》 CSCD 2020年第1期26-33,共8页
The summer western North Pacific subtropical high(WNPSH) has large influences on the East Asian summer climate. Many studies have focused on the projected changes in the WNPSH, but little is known about the changes un... The summer western North Pacific subtropical high(WNPSH) has large influences on the East Asian summer climate. Many studies have focused on the projected changes in the WNPSH, but little is known about the changes under different global warming targets, such as 1.5℃ and 2.0℃. This study investigates the changes in the WNPSH under six global warming targets(1.5℃, 2.0℃, 2.5℃,3.0℃, 3.5℃, and 4.0℃) in both the mid-and lower troposphere, using the outputs of CMIP5 model in historical simulations and under Representative Concentration Pathway 8.5. The projected changes in the WNPSH, which is measured by multiple variables, show that it changes little under the 1.5℃ target in the mid-troposphere, but weakens and retreats approximately 2.5° in longitude under the 2.0℃ target. It tends to linearly weaken with warming greater than 2.5℃ and shifts eastward by approximately 6.0° in longitude by the 4.0℃ target. Meanwhile, the WNPSH intensifies and extends westward under the 1.5℃ target in the lower troposphere, but changes little with warming rising from 1.5℃ to 2.0℃. It is projected to extend westward by approximately2.0° in longitude by the 4.0℃ target. 展开更多
关键词 Global warming target western North Pacific subtropical high climate change
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ACCURATE DETECTION OF HIGH-SPEED MULTI-TARGET VIDEO SEQUENCES MOTION REGIONS BASED ON RECONSTRUCTED BACKGROUND DIFFERENCE 被引量:1
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作者 Zhang Wentao Li Xiaofeng Li Zaiming (Inst. of Communication and Information, UEST of China, Chengdu 610054) 《Journal of Electronics(China)》 2001年第1期1-7,共7页
The paper first discusses shortcomings of classical adjacent-frame difference. Sec ondly, based on the image energy and high order statistic(HOS) theory, background reconstruction constraints are setup. Under the help... The paper first discusses shortcomings of classical adjacent-frame difference. Sec ondly, based on the image energy and high order statistic(HOS) theory, background reconstruction constraints are setup. Under the help of block-processing technology, background is reconstructed quickly. Finally, background difference is used to detect motion regions instead of adjacent frame difference. The DSP based platform tests indicate the background can be recovered losslessly in about one second, and moving regions are not influenced by moving target speeds. The algorithm has important usage both in theory and applications. 展开更多
关键词 MOTION DETECTION BACKGROUND reconstruction Image energy HOS high-SPEED target Block processing
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Numerical Study on the Two-Dimensional Temperature Fields of Titanium/Aluminum Double-Layer Target Irradiated by High-Intensity Pulsed Ion Beam
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作者 吴迪 雷明凯 +1 位作者 朱小鹏 宫野 《Plasma Science and Technology》 SCIE EI CAS CSCD 2010年第5期581-584,共4页
Interaction between high-intensity pulsed ion beam (HIPIB) and a double-layer target with titanium film on top of aluminum substrate was simulated. The two-dimensional nonlinear thermal conduction equations, with th... Interaction between high-intensity pulsed ion beam (HIPIB) and a double-layer target with titanium film on top of aluminum substrate was simulated. The two-dimensional nonlinear thermal conduction equations, with the deposited energy in the target taken as source term, were derived and solved by finite differential method. As a result, the two-dimensional spatial and temporal evolution profiles of temperature were obtained for a titanium/aluminum double-layer target irradiated by a pulse of HIPIB. The effects of ion beam current density on the phase state of the target materials near the film and substrate interface were analyzed. Both titanium and aluminum were melted near the interface after a shot when the ion beam current density fell in the range of 100 A/cm2 to 200 A/cm2. 展开更多
关键词 high-intensity pulsed ion beam double-layer target temperature fields numerical method
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CT-MRI同体位图像融合在高级别脑胶质瘤放射治疗靶区勾画中应用 被引量:1
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作者 金龙 杨振 +2 位作者 张鑫 刘晓斌 缪星宇 《生物医学工程与临床》 CAS 2024年第1期35-41,共7页
目的探讨同体位MRI图像(MRIsim)与CT模拟定位图像融合在高级别脑胶质瘤放射治疗靶区勾画中的临床应用价值。方法选择20例脑胶质瘤术后放射治疗患者,其中男性13例,女性7例;年龄39~69岁,平均年龄45.5岁;全部为单发病变;均已行全切或不全... 目的探讨同体位MRI图像(MRIsim)与CT模拟定位图像融合在高级别脑胶质瘤放射治疗靶区勾画中的临床应用价值。方法选择20例脑胶质瘤术后放射治疗患者,其中男性13例,女性7例;年龄39~69岁,平均年龄45.5岁;全部为单发病变;均已行全切或不全切手术,全部经病理组织诊断证实;世界卫生组织(WHO)分级Ⅲ级6例,Ⅳ级14例。将每例患者的同体位MRIsim、常规MRI影像(MRIconv)分别与CT模拟定位图像融合。运用Dice相似指数(DSC)和豪斯多夫距离(HD)算法来评价配准的精确度。在CT与MRIsim融合图像(Fusion-CT MRIsim)、CT与MRIconv融合图像(Fusion-CT MRIconv)上分别勾画危及器官(OAR)及靶区[大体肿瘤靶区(GTV)、临床肿瘤靶区(CTV)]。评估两种融合图像(即Fusion-CT MRIsim组和Fusion-CT MRIconv组)OAR勾画体积、GTV、CTV及剂量学差异。结果融合精确度评估:除全脑外,Fusion-CT MRIsim组其余OAR DSC均高于Fusion-CT MRIconv组(P<0.05);Fusion-CT MRIsim组OAR HD小于Fusion-CT MRIconv组(P<0.05)。OAR勾画体积比较:Fusion-CT MRIsim组OAR勾画体积与Fusion-CT MRIconv比较,差异无统计学意义(P>0.05)。靶区:Fusion-CT MRIsim组GTV、CTV小于Fusion-CT MRIconv组[(118.2±8.0)cm^(3)vs(125.3±8.1)cm^(3)、(234.3±12.8)cm^(3)vs(256.0±13.4)cm^(3)],差异有显著统计学意义(均P=0.000)。剂量学比较:Fusion-CT MRIsim组D_(max)-PTV、D_(mean)-PTV与Fusion-CT MRIconv组[(6432.9±23.0)cGy vs(6430.4±25.2)cGy、(6159.0±13.7)cGy vs(6166.2±17.3)cGy]比较,差异无统计学意义(P>0.05)。结论CT-MRI同体位融合图像配准精确度高,可降低全脑平均剂量(D_(mean))及缩小GTV及CTV,是高级别脑胶质瘤术后精确放射治疗值得广泛应用的临床方法。 展开更多
关键词 CT-MRI同体位融合 高级别胶质瘤 放射治疗靶区 靶区勾画
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基于高分辨一维距离像及其特征的空间目标识别效果分析 被引量:1
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作者 王放 韩晓磊 张延鑫 《电讯技术》 北大核心 2024年第3期465-469,共5页
目标高分辨一维距离像(High Resolution Range Profile,HRRP)中包含了丰富的目标尺寸、结构等目标特征,是进行空间目标身份识别的有效途径。但由于卫星宽带雷达实测数据获取难度大,前期相关研究多集中于基于HRRP的目标识别算法,结论也... 目标高分辨一维距离像(High Resolution Range Profile,HRRP)中包含了丰富的目标尺寸、结构等目标特征,是进行空间目标身份识别的有效途径。但由于卫星宽带雷达实测数据获取难度大,前期相关研究多集中于基于HRRP的目标识别算法,结论也多是基于仿真数据和少量类别(几类)的前提下得到的,与工程应用实际情况有较大差距,工程指导意义有限。为解决这一问题,基于地基雷达获取的30类卫星目标的大量一维距离像实测数据,从识别正确率的角度对目标HRRP及其特征(组合)的可分性和在空间目标个体识别中的应用效果进行了量化分析,分析结果可为后续基于HRRP的空间目标个体识别技术研究和工程应用提供可靠依据。 展开更多
关键词 空间目标 高分辨率一维距离像(HRRP) 目标识别 识别效果分析
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玉米花粉高温胁迫相关miRNA的筛选及其靶基因分析 被引量:2
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作者 李川 张盼盼 +4 位作者 张美微 牛军 穆蔚林 郭涵潇 乔江方 《河南农业科学》 北大核心 2024年第2期1-16,共16页
以高耐热玉米品种郑单958、低耐热玉米品种先玉335为材料,以正常生长条件为对照,在花期进行高温胁迫,通过miRNA高通量测序筛选玉米花粉中的差异表达miRNA,然后预测其靶基因,并对靶基因的本体特征和代谢通路进行富集分析。结果表明,共筛... 以高耐热玉米品种郑单958、低耐热玉米品种先玉335为材料,以正常生长条件为对照,在花期进行高温胁迫,通过miRNA高通量测序筛选玉米花粉中的差异表达miRNA,然后预测其靶基因,并对靶基因的本体特征和代谢通路进行富集分析。结果表明,共筛选到818个miRNA前体序列。在郑单958高温胁迫花粉与对照花粉对比组(HT958 vs CK958)中共筛选到19个显著差异表达miRNA序列,其中15个miRNA序列上调表达,4个下调表达,3个miRNA序列达到极显著水平(P<0.01)。对这19个显著差异表达miRNA的靶基因进行预测,共获得了503个基因转录本,其富集较多的GO生物学过程条目分别为转录调控DNA-模板、微管生物学过程、磷酸化作用、RNA聚合酶Ⅱ正向调控转录过程、甲基化作用等,KEGG富集较显著的代谢通路分别是谷胱甘肽代谢、碳代谢、花生四烯酸代谢、糖酵解/糖异生、叶酸生物合成等。在先玉335高温胁迫花粉与对照花粉对比组(HT335 vs CK335)中共筛选到15个显著差异表达miRNA序列,其中7个miRNA序列上调表达,8个下调表达,1个miRNA序列达到了极显著水平(P<0.01)。对这15个显著差异表达miRNA的靶基因进行预测,共获得了454个基因转录本,其富集较多的GO生物学过程条目分别为转录调控DNA-模板、磷酸化作用、蛋白质磷酸化作用、蛋白质水解、DNA修复等,富集较显著的KEGG代谢通路分别是其他多糖降解、亚油酸代谢、代谢通路、硫胺素代谢、内质网内蛋白质加工过程等。在郑单958高温胁迫花粉与先玉335高温胁迫花粉对比组(HT985 vs HT335)中共筛选到85个显著差异表达miRNA序列,其中35个miRNA序列为上调表达,50个为下调表达,24个miRNA序列达到了极显著水平(P<0.01)。对这85个显著差异表达miRNA的靶基因进行预测,共获得了2 286个基因转录本,其富集较多的GO生物学过程条目分别为转录调控DNA-模板、磷酸化作用、蛋白质磷酸化、蛋白质水解、跨膜转运等,富集较显著的代谢通路分别是鞘脂类代谢、淀粉和蔗糖代谢、其他多糖降解、代谢通路、半胱氨酸及甲硫氨酸代谢等。在HT958 vs CK958与HT335 vs CK335对比组中共筛选到94个显著差异表达miRNA序列,其中(预测全新)PC-3p-10069_1143、(预测全新)PC-3p-18335_646、(玉米)zma-miR164f-5p等28个miRNA序列达到了极显著水平(P<0.01)。对这94个显著差异表达miRNA的靶基因进行预测,共获得了4 569个基因转录本,其富集较多的GO生物学过程条目分别为转录调控DNA-模板、磷酸化作用、蛋白质磷酸化、蛋白质转运、蛋白质水解等,其富集较显著的KEGG代谢通路分别是内质网内蛋白质加工过程、真核生物核糖体生物合成、剪接体、鞘脂类代谢、内吞作用等。 展开更多
关键词 玉米 花粉 花期高温胁迫 miRNA测序 差异表达miRNA 靶基因
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淫羊藿苷调控mTOR/Akt/CREB通路对高糖诱导的足细胞自噬及凋亡的影响 被引量:3
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作者 李明霞 杨谦 +4 位作者 乔海霞 王晓玲 贾丽媛 胡利梅 任卫东 《医药导报》 CAS 北大核心 2024年第1期19-25,共7页
目的 探讨淫羊藿苷对高糖诱导的足细胞自噬、凋亡及哺乳动物雷帕霉素靶蛋白(mTOR)/丝氨酸苏氨酸蛋白激酶(Akt)/环磷酸腺苷反应元件结合蛋白(CREB)通路的影响。方法 将小鼠足细胞MPC5分为5组:正常对照组(5.5 mmol·L^(-1)葡萄糖)、... 目的 探讨淫羊藿苷对高糖诱导的足细胞自噬、凋亡及哺乳动物雷帕霉素靶蛋白(mTOR)/丝氨酸苏氨酸蛋白激酶(Akt)/环磷酸腺苷反应元件结合蛋白(CREB)通路的影响。方法 将小鼠足细胞MPC5分为5组:正常对照组(5.5 mmol·L^(-1)葡萄糖)、高糖组(30 mmol·L^(-1)葡萄糖)、淫羊藿苷组(30 mmol·L^(-1)葡萄糖+5μmol·L^(-1)淫羊藿苷)、GDC-0349组(30 mmol·L^(-1)葡萄糖+50μmol·L^(-1)GDC-0349)、淫羊藿苷+GDC-0349组(30 mmol·L^(-1)葡萄糖+5μmol·L^(-1)淫羊藿苷+50μmol·L^(-1)GDC-0349)。培养48 h后,噻唑蓝法检测MPC5细胞活力;吖啶橙染色观察MPC5细胞自噬情况;流式细胞术检测MPC5细胞凋亡;蛋白印迹法检测MPC5细胞自噬[微管相关蛋白1轻链3(LC3)Ⅱ、LC3Ⅰ、自噬相关蛋白(Beclin-1)]、凋亡[Bcl-2相关X蛋白(Bax)、B淋巴细胞瘤-2(Bcl-2)]和mTOR/Akt/CREB通路相关蛋白的表达。结果 与正常对照组比较,高糖组MPC5细胞活力、Bcl-2、磷酸化mTOR(p-mTOR)/mTOR、磷酸化Akt(p-Akt)/Akt、磷酸化CREB(p-CREB)/CREB蛋白表达水平显著降低(P<0.05),自噬能力增强,自噬体表现出橙色荧光,细胞凋亡率、LC3Ⅱ/LC3Ⅰ、Beclin-1、Bax蛋白表达水平显著升高(P<0.05)。与高糖组比较,淫羊藿苷组MPC5细胞活力、LC3Ⅱ/LC3Ⅰ、Beclin-1、Bcl-2、p-mTOR/mTOR、p-Akt/Akt、p-CREB/CREB蛋白表达水平显著升高,自噬能力进一步增强,自噬体数量增多,自噬体呈现出砖红色荧光(P<0.05),细胞凋亡率、Bax蛋白表达水平显著降低(P<0.05);GDC-0349组MPC5细胞活力、LC3Ⅱ/LC3Ⅰ、Beclin-1、Bcl-2、p-mTOR/mTOR、p-Akt/Akt、p-CREB/CREB蛋白表达水平显著降低,自噬能力减弱,自噬体数量减少,自噬体表现出橙色荧光(P<0.05),细胞凋亡率、Bax蛋白表达水平显著升高(P<0.05);淫羊藿苷+GDC-0349可逆转淫羊藿苷对高糖诱导MPC5细胞的作用效果(P<0.05)。结论 淫羊藿苷通过激活mTOR/Akt/CREB通路促进高糖诱导的足细胞自噬抑制细胞凋亡。 展开更多
关键词 淫羊藿苷 哺乳动物雷帕霉素靶蛋白 蛋白激酶B 环磷酸腺苷反应元件结合蛋白 高糖 足细胞 自噬 凋亡
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下调HMGB2表达对肝癌LM3细胞上皮-间质转化的抑制作用及其AKT/mTOR信号通路机制 被引量:1
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作者 魏雁虹 杨晨雪 +4 位作者 杨广民 宋帅 李明 杨海娇 魏海峰 《吉林大学学报(医学版)》 CAS CSCD 北大核心 2024年第1期143-149,共7页
目的:探讨下调肝癌细胞中高迁移率族框蛋白2 (HMGB2)表达对肝癌细胞生物学行为及上皮-间质转化(EMT)进程的影响,并阐明其作用机制。方法:对数生长期的人肝癌LM3细胞分为阴性对照组和HMGB2 RNA干扰组(HMGB2 siRNA组),分别以Lipofectamin ... 目的:探讨下调肝癌细胞中高迁移率族框蛋白2 (HMGB2)表达对肝癌细胞生物学行为及上皮-间质转化(EMT)进程的影响,并阐明其作用机制。方法:对数生长期的人肝癌LM3细胞分为阴性对照组和HMGB2 RNA干扰组(HMGB2 siRNA组),分别以Lipofectamin 2000为载体转染无关序列的RNA寡核苷酸(RNA oligo)和敲除HMGB2序列的RNA oligo。采用实时荧光定量PCR(RT-qPCR)法和Western blotting法检测2组细胞中HMGB2 mRNA和蛋白表达水平,分别采用细胞划痕实验和Transwell小室实验检测2组细胞的迁移和侵袭能力,采用Western blotting法检测2组细胞中E-钙黏蛋白(E-cadherin)、 N-钙黏蛋白(N-cadherin)、波形蛋白(Vimentin)和蛋白激酶B(AKT)/哺乳动物雷帕霉素靶蛋白(mTOR)通路相关蛋白表达水平。结果:与阴性对照组比较,HMGB2 siRNA组细胞中HMGB2 mRNA和蛋白表达水平均明显降低(P<0.05),HMGB2 siRNA组细胞划痕愈合率明显降低(P<0.01),侵袭细胞数明显减少(P<0.01),细胞中E-cadherin蛋白表达水平明显升高(P<0.01),N-cadherin、Vimentin、mTOR、AKT和磷酸化AKT (p-AKT)蛋白表达水平明显降低(P<0.05或P<0.01)。结论:下调HMGB2的表达可降低肝癌LM3细胞迁移和侵袭能力并抑制EMT,其作用机制可能与参与调节AKT/mTOR通路相关蛋白表达有关。 展开更多
关键词 肝肿瘤 高迁移率族框蛋白2 上皮-间质转化 细胞迁移 细胞侵袭 蛋白激酶B/哺乳动物雷帕霉素靶蛋白
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拍瓦激光驱动纳米刷靶高品质质子束的产生
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作者 杨月 孙斌 +16 位作者 邓志刚 滕建 贺书凯 张博 王少义 张智猛 于明海 温家星 谭放 闫永宏 王子涛 周凯南 陈忠靖 吴玉迟 周维民 谷渝秋 赵宗清 《强激光与粒子束》 CAS CSCD 北大核心 2024年第1期112-116,共5页
超强激光加速产生的高能质子束源在基础物理研究、材料科学、生物医疗等领域具有广泛应用前景。基于激光聚变研究中心的SILEX-II装置,开展了高对比度飞秒激光驱动纳米刷靶质子加速实验研究。采用等离子体镜技术进一步提升激光对比度,有... 超强激光加速产生的高能质子束源在基础物理研究、材料科学、生物医疗等领域具有广泛应用前景。基于激光聚变研究中心的SILEX-II装置,开展了高对比度飞秒激光驱动纳米刷靶质子加速实验研究。采用等离子体镜技术进一步提升激光对比度,有效降低了预脉冲对纳米刷靶结构的影响。相比于平面靶,采用纳米刷靶质子截止能量提高到1.5倍,质子束产额增加近一个量级,成功验证了超高功率密度下纳米刷靶对激光离子加速的增强效果,并且有效提升了质子束空间分布的均匀性。研究结果为高品质质子束源的产生和应用提供了技术途径。 展开更多
关键词 纳米刷靶 激光离子加速 等离子体镜 高品质质子束
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高机动目标的改进强跟踪CKF自适应IMM算法
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作者 成怡 刘铭阳 徐国伟 《中国惯性技术学报》 EI CSCD 北大核心 2024年第7期715-723,共9页
为提升高机动目标跟踪精度,提出了一种改进的强跟踪CKF自适应交互多模型跟踪算法。在IMM算法运动模型集中引入CS-Jerk模型,增强对高机动目标的适应能力,采用奇异值分解(SVD)算法解决模型集中因模型扩维而导致CKF算法无法Cholesky分解的... 为提升高机动目标跟踪精度,提出了一种改进的强跟踪CKF自适应交互多模型跟踪算法。在IMM算法运动模型集中引入CS-Jerk模型,增强对高机动目标的适应能力,采用奇异值分解(SVD)算法解决模型集中因模型扩维而导致CKF算法无法Cholesky分解的问题;提出了一种改进的强跟踪CKF算法,降低强跟踪CKF算法的计算量;利用模型的后验信息对IMM算法模型转移概率进行自适应调整,提高跟踪精度。仿真结果表明,基于所提算法目标的位置均方根误差均值和速度均方根误差均值较IMM-CKF算法分别降低了22.50%和16.58%,有效提高了目标跟踪精度。 展开更多
关键词 高机动目标 目标跟踪 自适应交互多模型 强跟踪CKF SVD分解
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公立医院实施科室目标责任制管理的实践与思考
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作者 于洪钊 赵茜倩 +2 位作者 田园 王冕 孙燕楠 《中国卫生标准管理》 2024年第6期39-43,共5页
公立医院是我国医疗服务体系的基础,公立医院的改革始终是深化卫生体制改革的重要内容,而加强公立医院运营管理是其中的重要一环。为了加强公立医院运营管理,实现这些战略发展目标,有必要加强内部绩效评估,以目标管理责任制为抓手,建立... 公立医院是我国医疗服务体系的基础,公立医院的改革始终是深化卫生体制改革的重要内容,而加强公立医院运营管理是其中的重要一环。为了加强公立医院运营管理,实现这些战略发展目标,有必要加强内部绩效评估,以目标管理责任制为抓手,建立内部综合绩效评估指标体系。文章从某公立医院实施临床医技科室目标责任制管理的实践入手,总结了实施目标责任管理的实施流程、指标体系构建原则和取得成效,对进一步做好目标责任制管理工作,进而做好公立医院绩效考核工作,深化公立医院运营管理提出了思考,为科学引领公立医院健康、稳定、高质量发展提供借鉴和依据。 展开更多
关键词 公立医院 目标责任制 绩效 考核 运营 高质量发展
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基于语义引导层次化分类的雷达地面目标HRRP识别方法
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作者 李阳 刘艺辰 +1 位作者 张亮 王彦华 《信号处理》 CSCD 北大核心 2024年第1期126-137,共12页
高分辨距离像(HRRP)反映了目标空间散射结构在雷达视线方向的投影,近年来被认为是地面目标识别的重要途径。现有的HRRP识别方法采用手工特征加传统机器学习分类器,均属于平面分类方法,即采用统一标准不加区别的优选特征并单次决策最终... 高分辨距离像(HRRP)反映了目标空间散射结构在雷达视线方向的投影,近年来被认为是地面目标识别的重要途径。现有的HRRP识别方法采用手工特征加传统机器学习分类器,均属于平面分类方法,即采用统一标准不加区别的优选特征并单次决策最终类别。然而该方法在实际应用中面临种类繁杂、数据不平衡、HRRP姿态敏感性等诸多问题,难以获取最佳的应用效果。层次化方法采取分而治之思想,将一个复杂的细粒度识别任务拆解为多个简单的识别子任务。本文采用层次化识别的思路,提出了一种基于语义引导层次化分类的雷达地面目标识别方法。该方法以联合语义和数据构建的树形结构将一个复杂的细粒度识别任务拆解为多个简单的识别子任务,并针对每一个识别子任务匹配一套优选特征集和一个局部分类器。本方法在仿真数据和实测数据上完成了验证。实验结果表明了本文方法处理地面目标识别任务的有效性。 展开更多
关键词 雷达目标识别 高分辨距离像 层次化分类
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融合高精度地图的多激光雷达路侧智能感知方法
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作者 胡钊政 陈琪莉 +2 位作者 孟杰 胡华桦 张佳楠 《交通信息与安全》 CSCD 北大核心 2024年第3期42-52,共11页
在车路协同路侧感知研究中,由于点云数据量庞大且存在着不可避免的目标遮挡情况,导致检测效率低、目标轨迹不稳定和跟踪精度低的问题。针对上述问题,提出了1种融合高精度地图的多激光雷达路侧智能感知方法,通过融合高精度地图信息,提高... 在车路协同路侧感知研究中,由于点云数据量庞大且存在着不可避免的目标遮挡情况,导致检测效率低、目标轨迹不稳定和跟踪精度低的问题。针对上述问题,提出了1种融合高精度地图的多激光雷达路侧智能感知方法,通过融合高精度地图信息,提高感知结果的准确性和可靠性。该方法分为3个部分:①通过多激光雷达的标定结果,利用高精度地图完成三维点云区域中感兴趣区域的提取,从而有效减少待处理点云的数量,提升计算效率;②基于极化图高斯混合背景模型的背景建模方法,利用极化图完成运动目标快速检测,避免大规模激光点云的直接处理,有效提升检测效率;③利用车辆航向与车道线方向一致性约束,将高精度地图中的车道方向转化为卡尔曼滤波框架下的车辆状态线性约束,改善车辆检测与轨迹跟踪的性能。实验中,分别在仿真交叉路口与实车实验道路双T形路口对算法与模型进行测试验证。相比于其他方法,所提出的方法数据量减少了55%,目标检测准确率提高了12%,耗时减少了56%,目标跟踪的误差极值、误差均值以及均方根误差均有所降低。实验结果表明:所提的方法能有效融合高精度地图信息,在大范围道路场景下实现对道路运动目标的快速检测与稳定跟踪。 展开更多
关键词 智能交通 路侧感知 目标跟踪 卡尔曼滤波 高精度地图 极化图
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