[Objectives]This study was conducted to investigate the relationship between HSPs and the response of high temperature stress.[Methods]Molecular biological techniques were applied to clone and analyze the gene sequenc...[Objectives]This study was conducted to investigate the relationship between HSPs and the response of high temperature stress.[Methods]Molecular biological techniques were applied to clone and analyze the gene sequence of DpHsp70 gene.The changes in the expression of DpHsp70 gene under high temperature stress and exogenous salicylic acid(SA)were observed and further analyzed by qRT PCR.[Results]The coding region of the Dahlia DpHsp70 gene was 705 bp,encoding 234 amino acid residues(GenBank accession number:MH102288).Aligned with Compositae plants,the Dahlia DpHsp70 gene shared more than 83%homology in gene sequence while 99%-100%homology in amino acid sequence.Under the 35℃high temperature stress,the expression of DpHsp70 gene in Dahlia petals significantly increased.Meanwhile,the expression of DpHsp70 gene further increased under SA at 35℃temperature,which was significantly higher than those of the control group and the 35℃high temperature stress treatment group.It was demonstrated that the Hsp70 gene in Compositae is with highly conservative property and its expression could be up-regulated in response to high temperature stress.It can also be concluded that applying exogenous SA can improve the high temperature resistance of Dahlia.[Conclusions]This study provides a new experimental basis for elucidating the physiological function and mechanism of Dahlia in response to high temperature stress.展开更多
为探究杜鹃花热激蛋白90(heat shock protein 90,Hsp90)在植物生长发育调节和高温胁迫响应中的作用,本研究以杜鹃花属中观赏价值高、环境适应性强的马银花(Rhododendron ovatum)为材料,利用生物信息学方法对其Hsp90家族开展全基因组水...为探究杜鹃花热激蛋白90(heat shock protein 90,Hsp90)在植物生长发育调节和高温胁迫响应中的作用,本研究以杜鹃花属中观赏价值高、环境适应性强的马银花(Rhododendron ovatum)为材料,利用生物信息学方法对其Hsp90家族开展全基因组水平鉴定以及基因结构、顺式作用元件、进化、表达模式等分析。结果表明马银花Hsp90家族有11个成员,分布在5条染色体上。启动子区顺式作用元件分析表明,Hsp90家族成员均参与植物激素代谢和逆境胁迫响应过程。利用马银花、拟南芥、番茄、茶、杜鹃、河南杜鹃、马缨杜鹃的Hsp90家族构建的系统进化树可被划分为4个主要分支。进化分析显示,Hsp90家族在杜鹃花属分化过程中受到纯化选择作用。Hsp90家族在不同组织器官中和高温处理下的表达模式表明,马银花Hsp90家族参与了花器官的发育和植物对高温胁迫的响应。本研究为杜鹃花Hsp90基因功能的深入解析奠定了基础。展开更多
植物热激蛋白是一类代表性高温响应蛋白。以玉米种质POB21为材料,克隆了一个CDS序列长度为477 bp的小分子热激蛋白基因。该基因编码的蛋白含158个氨基酸,具有HSP20蛋白典型的ACD结构域,预测的等电点为5.36,分子量为17.746 k D,被命名为Z...植物热激蛋白是一类代表性高温响应蛋白。以玉米种质POB21为材料,克隆了一个CDS序列长度为477 bp的小分子热激蛋白基因。该基因编码的蛋白含158个氨基酸,具有HSP20蛋白典型的ACD结构域,预测的等电点为5.36,分子量为17.746 k D,被命名为Zm HSP17.7。在水稻、拟南芥等植物基因组中都有其同源基因,进化和亚细胞定位分析表明,此基因属CI类小分子热激蛋白家族成员。Northern杂交分析表明,高温快速诱导Zm HSP17.7基因表达,15%PEG模拟干旱胁迫不诱导该基因表达,但在复合胁迫下干旱增强了高温的诱导效果。外源ABA也不影响该基因的表达。与野生型拟南芥相比,超表达Zm HSP17.7的转基因拟南芥在种子萌发和植株生长过程中表现出更强的高温和干旱耐受性,说明该基因可以在植物防御高温、干旱及复合胁迫中发挥一定作用。展开更多
基金Supported by Applied Basic Research Program of Suzhou City,Jiangsu Province(SYN201405).
文摘[Objectives]This study was conducted to investigate the relationship between HSPs and the response of high temperature stress.[Methods]Molecular biological techniques were applied to clone and analyze the gene sequence of DpHsp70 gene.The changes in the expression of DpHsp70 gene under high temperature stress and exogenous salicylic acid(SA)were observed and further analyzed by qRT PCR.[Results]The coding region of the Dahlia DpHsp70 gene was 705 bp,encoding 234 amino acid residues(GenBank accession number:MH102288).Aligned with Compositae plants,the Dahlia DpHsp70 gene shared more than 83%homology in gene sequence while 99%-100%homology in amino acid sequence.Under the 35℃high temperature stress,the expression of DpHsp70 gene in Dahlia petals significantly increased.Meanwhile,the expression of DpHsp70 gene further increased under SA at 35℃temperature,which was significantly higher than those of the control group and the 35℃high temperature stress treatment group.It was demonstrated that the Hsp70 gene in Compositae is with highly conservative property and its expression could be up-regulated in response to high temperature stress.It can also be concluded that applying exogenous SA can improve the high temperature resistance of Dahlia.[Conclusions]This study provides a new experimental basis for elucidating the physiological function and mechanism of Dahlia in response to high temperature stress.
文摘为探究杜鹃花热激蛋白90(heat shock protein 90,Hsp90)在植物生长发育调节和高温胁迫响应中的作用,本研究以杜鹃花属中观赏价值高、环境适应性强的马银花(Rhododendron ovatum)为材料,利用生物信息学方法对其Hsp90家族开展全基因组水平鉴定以及基因结构、顺式作用元件、进化、表达模式等分析。结果表明马银花Hsp90家族有11个成员,分布在5条染色体上。启动子区顺式作用元件分析表明,Hsp90家族成员均参与植物激素代谢和逆境胁迫响应过程。利用马银花、拟南芥、番茄、茶、杜鹃、河南杜鹃、马缨杜鹃的Hsp90家族构建的系统进化树可被划分为4个主要分支。进化分析显示,Hsp90家族在杜鹃花属分化过程中受到纯化选择作用。Hsp90家族在不同组织器官中和高温处理下的表达模式表明,马银花Hsp90家族参与了花器官的发育和植物对高温胁迫的响应。本研究为杜鹃花Hsp90基因功能的深入解析奠定了基础。
文摘目的:分析薄荷幼苗高温胁迫下的差异基因表达情况,从中挖掘薄荷幼苗的耐热功能基因,为薄荷幼苗的抗逆机制提供生物信息学依据。方法:以水培薄荷为研究对象,分别进行高温胁迫0 h(对照,CK)、3 h(40℃,HT-3 h)和6 h(40℃,HT-6 h)的3种处理,然后进行转录组测序,对比分析筛选出高温胁迫应答的基因,并对部分基因利用实时荧光定量PCR(qRT-PCR)进行验证。结果:HT-3 h vs CK和HT-6 h vs CK的共同差异表达基因(Differential expressed genes,DEGs)有1042个,其中上调基因590个,下调基因452个。对共有DEGs进行GO功能富集分析,以对p值矫正后的FDR≤0.05为阈值,得出55条GO terms功能组为显著富集,主要体现在生物过程(Biological Process,BP)方面,包括活性氧反应、对刺激的反应、热反应等;KEGG功能富集分析得出,共有611个DEGs映射到171条通路当中,主要涉及代谢途径、内质网中的蛋白质加工、核糖体、次级代谢产物的生物合成等方面。进一步挖掘响应HT-3 h和HT-6 h的共有DEGs中,上调的有33个Hsp家族的基因,主要包括19个Hsp20基因,5个Hsp90基因和7个HSPAls基因等;另有5个转录因子BES1、GRAS、HB-HD-ZIP、MYB-related、NAC亦显示上调。利用qRT-PCR对测序数据进行验证,基因的表达趋势与转录组测序结果一致,证明测序结果真实可靠。结论:薄荷通过启动活性氧代谢或氧化应激等生物进程来响应高温胁迫,并通过内质网中的蛋白质加工等途径来抵御高温伤害。上调热激蛋白Hsp20、Hsp90、HSPAls等基因和BES1、GRAS、HB-HD-ZIP、MYB-related、NAC等5个转录因子家族的相关基因,对薄荷的耐热调控具有积极意义。
