AIM:To evaluate effects of endogenous dopamine induced by low concentration atropine eye drops on choroidal neovascularization(CNV)in high myopia mice.METHODS:The C57BL/6J mice were deprived of the right eye for 4wk,a...AIM:To evaluate effects of endogenous dopamine induced by low concentration atropine eye drops on choroidal neovascularization(CNV)in high myopia mice.METHODS:The C57BL/6J mice were deprived of the right eye for 4wk,and the high myopia was diagnosed by optometry,the diopter was less than-6.00 D,and CNV was induced by 532 nm laser.The changes of dopamine D1 receptor(DRD1),dopamine D2 receptor(DRD2),and vascular endothelial growth factor A(VEGFA)were detected by Western blot technology at 0.5,1,2h,and 7d after 0.01%,0.05%,and 0.1%atropine eye drops,respectively,the area of CNV was measured.RESULTS:Significant increases were observed on the expression of DRD2 in mouse high myopia model at 0.5,1,2h,7d with 0.05%and 0.1%atropine eye drops(P<0.05).Significant decreases were observed on the expression of DRD1 and VEGFA in mouse high myopia model at 0.5,1,2h,7d with 0.05%and 0.1%atropine eye drops(P<0.05).The area of CNV induced by laser in the drug-treated group was significantly smaller than that in the control group,and the higher the concentration,the more significant the inhibitory effect(P<0.05).CONCLUSION:The 0.01%,0.05%,0.1%atropine eye drops can decrease the level of VEGFA and inhibit high myopia CNV indirectly by up-regulating the level of DRD2 and down-regulating the level of DRD1,and the effect of 0.05%and 0.1%atropine eye drops is more significant.展开更多
A new technological process for production of talium tungstate from low-grade tungsten-concentrate witha high content of calcium and other impurities has been studied. The experiments showed that average tungstenleach...A new technological process for production of talium tungstate from low-grade tungsten-concentrate witha high content of calcium and other impurities has been studied. The experiments showed that average tungstenleaching efficiency of more than 96. 92 % can be obtained with a low NaOH consumption by using the mechani-cal activating caustic decomposition , and the content of main impurities (P, As, Si) in Na_2WO_4 solution ob-tained is competitive with that from standard wolframite concentrate by traditional caustic decomposition. Afterrecovering caustic liquor by first crystallization, molylxlenum is removed from Na_2WO_4 solution by ion ex-change method. High-purity sodium tungstate is obtained by second crystallization of Na_2WO_4 solution. Thistechnology has the advantages of good adaptability for raw materials, high removing efficiency of impurities,high tungsten recovery and high economic benefit.展开更多
文摘AIM:To evaluate effects of endogenous dopamine induced by low concentration atropine eye drops on choroidal neovascularization(CNV)in high myopia mice.METHODS:The C57BL/6J mice were deprived of the right eye for 4wk,and the high myopia was diagnosed by optometry,the diopter was less than-6.00 D,and CNV was induced by 532 nm laser.The changes of dopamine D1 receptor(DRD1),dopamine D2 receptor(DRD2),and vascular endothelial growth factor A(VEGFA)were detected by Western blot technology at 0.5,1,2h,and 7d after 0.01%,0.05%,and 0.1%atropine eye drops,respectively,the area of CNV was measured.RESULTS:Significant increases were observed on the expression of DRD2 in mouse high myopia model at 0.5,1,2h,7d with 0.05%and 0.1%atropine eye drops(P<0.05).Significant decreases were observed on the expression of DRD1 and VEGFA in mouse high myopia model at 0.5,1,2h,7d with 0.05%and 0.1%atropine eye drops(P<0.05).The area of CNV induced by laser in the drug-treated group was significantly smaller than that in the control group,and the higher the concentration,the more significant the inhibitory effect(P<0.05).CONCLUSION:The 0.01%,0.05%,0.1%atropine eye drops can decrease the level of VEGFA and inhibit high myopia CNV indirectly by up-regulating the level of DRD2 and down-regulating the level of DRD1,and the effect of 0.05%and 0.1%atropine eye drops is more significant.
文摘A new technological process for production of talium tungstate from low-grade tungsten-concentrate witha high content of calcium and other impurities has been studied. The experiments showed that average tungstenleaching efficiency of more than 96. 92 % can be obtained with a low NaOH consumption by using the mechani-cal activating caustic decomposition , and the content of main impurities (P, As, Si) in Na_2WO_4 solution ob-tained is competitive with that from standard wolframite concentrate by traditional caustic decomposition. Afterrecovering caustic liquor by first crystallization, molylxlenum is removed from Na_2WO_4 solution by ion ex-change method. High-purity sodium tungstate is obtained by second crystallization of Na_2WO_4 solution. Thistechnology has the advantages of good adaptability for raw materials, high removing efficiency of impurities,high tungsten recovery and high economic benefit.
