The present work focused on the high expression of recombinant human consensus interferon-α(cIFN)by Pichia pastoris.The cycle of glycerol feeding,the strategy of methanol feeding and the optimum pH for protein induct...The present work focused on the high expression of recombinant human consensus interferon-α(cIFN)by Pichia pastoris.The cycle of glycerol feeding,the strategy of methanol feeding and the optimum pH for protein induction were studied.The optimized strategies were a 4-h glycerol-feeding period,induction pH being kept at 5.0 and methanol concentration being kept under 5 g/L.The maximum dry cell weight,cIFN production and bioactivity obtained were 168,1.24 g/L and 5.4×10^(7) U/mL,respectively.展开更多
基金This work was supported by a key grant from Science and Technology Commission of Shanghai Municipality(Grant No.05DZ19326)Shuguang Genzong Program supported by Shanghai Municipal Education Commission(Grant No.06GG07).
文摘The present work focused on the high expression of recombinant human consensus interferon-α(cIFN)by Pichia pastoris.The cycle of glycerol feeding,the strategy of methanol feeding and the optimum pH for protein induction were studied.The optimized strategies were a 4-h glycerol-feeding period,induction pH being kept at 5.0 and methanol concentration being kept under 5 g/L.The maximum dry cell weight,cIFN production and bioactivity obtained were 168,1.24 g/L and 5.4×10^(7) U/mL,respectively.
