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Prevalence of Precancerous Lesions Based on Digital Cervicography with VIA/VILI among Women Positive for High-Risk Human Papillomavirus Serotypes: A Screening Center-Based Study in Cameroon
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作者 Jeffrey-Lewis Nzang Cliford Ebontane Ebong +8 位作者 Simon Manga Florence Manjuh Félix Essiben Isidore Tompeen Judith Seungue Serge Robert Nyada Jeanne Hortence Fouedjio Ymele Fouelifack Julius Sama Dohbit 《Open Journal of Obstetrics and Gynecology》 2024年第6期967-978,共12页
Background: Since 2021, high-risk Human Papilloma Virus (HR-HPV) testing has been the recommended screening test for cervical cancer for all settings;either used alone in a “test and treat” strategy, or with a triag... Background: Since 2021, high-risk Human Papilloma Virus (HR-HPV) testing has been the recommended screening test for cervical cancer for all settings;either used alone in a “test and treat” strategy, or with a triage test, with or without biopsy, before treatment. Cameroon has rolled out immunization against HPV 16 and 18, but studies show a higher prevalence of non-16/18 HR-HPV types. Objectives: Determine the prevalence of precancerous lesions, in women with HR-HPV infection and evaluate association of digital cervicography (DC) VIA/VILI positivity with HPV serotype, as a measure of their contribution to precancer and cancer incidence. Methodology: The study was cross-sectional, descriptive, and analytic. It took place at the Etoug-Ebe and Ekoudoum Baptist Hospitals in Yaoundé, during the period April-September 2022. We reviewed the records of women screened for cervical cancer between February 2020 and December 2021 and evaluated the prevalence of lesions on digital cervicography (DC) with VIA/VILI for women positive for HR-HPV serotypes. The data were analyzed using SPSS version 20.0 for Windows. P values Results: We identified 315 cases with a positive HR-HPV deoxyribonucleic acid (DNA) test, 224 (71.1%) had a DC VIA/VILI triage test done. Of these, 30 (13.4%) women had a positive DC VIA/VILI, with five women (2.2%) having lesions suggestive of cancer. Out of 11 cases positive for HPV 16 alone, 05 (45.5%) had a positive DC VIA/VILI test. Of the 14 cases positive for HPV 18 alone, 03 (21.4%) had a positive VIA/VILI, meanwhile only 19 (10.7%) of the 177 cases positive for non-16/18 HPV had a positive VIA/VILI test. Conclusion: A high proportion of women (13.4%) with HR HPV had a positive DC VIA/VILI, with a significant proportion (2.2%) having lesions suggestive of invasive cervical cancer HR-HPV serotype was associated with DC VIA/VILI positivity;HPV 16 had the strongest association (45.5%), followed by HPV 18 (21.4%), and non-16/18 HR-HPV (10.7%), suggesting a decreasing order of oncogenicity. 展开更多
关键词 high-risk human Papillomavirus PRECANCEROUS Digital Cervicography VIA/VILI SEROTYPE
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Distribution of High-Risk Human Papillomavirus Genotypes among Women with Colposcopic Diagnosis of Cervical Intraepithelial Neoplasia in Bangladesh
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作者 Siddika Mosammat Shahida Mina Chowdhury +4 位作者 Fatima Shajahan Jannat Ara Rifat Alfi Sharin Lubaba S. M. Shamsuzzaman Annekathryn Goodman 《Journal of Cancer Therapy》 2023年第6期277-290,共14页
Background: The incidence of cervical cancer is high in Bangladesh and there is a high prevalence of preinvasive lower genital tract disease among women of reproductive age. Persistent high-risk Human Papilloma Virus ... Background: The incidence of cervical cancer is high in Bangladesh and there is a high prevalence of preinvasive lower genital tract disease among women of reproductive age. Persistent high-risk Human Papilloma Virus (HPV) infection is the main underlying cause of cervical cancer and its precursor, cervical intraepithelial neoplasia (CIN). Objective: The aim of the study was to identify the subtypes of high-risk HPV infection among women with the colposcopic diagnosis of cervical intraepithelial neoplasia in Bangladesh. Methods: This cross-sectional observational study was conducted in the colposcopy clinic of Dhaka Medical College Hospital over a six-month period. A total of 100 participants were enrolled. Married women, between 30 - 60 years of age with colposcopically diagnosed cervical intra epithelial neoplasia were enrolled. Women with chronic illness, pregnancy, and women unable to consent were excluded from this study. After counselling, colposcopically directed punch biopsies were taken from each CIN case concurrently with high-risk HPV testing by polymerase chain reaction (PCR). Results: The mean age of the patients was 38.69 (SD ±7.76) years. CIN 1 was diagnosed in 57% of participants, while 24% had CIN II and 19% had CIN III lesions. High-risk HPV was present in 52 patients. HPV 16 was the most common identified in 28 (53.84%) and HPV 18 was the second most common with 20 (38.46%) either singly or in combination with other high-risk subtypes. The other HPV strains, HPV 31, 33, 35, 52, 56 and 58, were also detected either as mono or co-infections. Out of the 52 HPV positive cases, 29 (55.8%) had mono infection and 23 (44.2%) had co-infection with several subtypes. The highest incidence (50%) of oncogenic HPV infections was present among women aged 35 - 45 years. Risk factors associated with HPV positive cases were high parity (P 0.05), early age at marriage (P = 0.754) and early age of first child. Conclusion: This study identified a high prevalence of HPV 16 and 18 genotypes. HPV vaccination with the current 9-valent HPV vaccine, which contains HPV types 6, 11, 16, 18, 31, 33, 45, 52, and 58. Will be an effective public health measure to eradicate cervical cancer in Bangladesh. 展开更多
关键词 BANGLADESH Cervical Cancer Cervical Intraepithelial Neoplasia human Papillomavirus Infection high-risk HPV
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Prevalence and Determinants of High-risk HPV Infection among 11549 Women from an Opportunistic Screening in Hubei Province 被引量:6
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作者 Quan-fu MA Yu-lin GUO +8 位作者 Han GAO Bin YAN Xuan DAI Meng XU Yu-jing XIONG Qiu-zi PENG Ying WANG Miao ZOU Xu-feng WU 《Current Medical Science》 SCIE CAS 2019年第4期622-630,共9页
High-risk human papillomavirus (hrHPV) infection plays an important role in the development of cervical intraepithelial neoplasia and cervical cancer.A total of 11 549 women were enrolled from the Maternal and Child H... High-risk human papillomavirus (hrHPV) infection plays an important role in the development of cervical intraepithelial neoplasia and cervical cancer.A total of 11 549 women were enrolled from the Maternal and Child Health Hospital of Hubei Province.Each participant accepted hrHPV testing and completed a self^administered questionnaire about basic information and potential risk factors.The univariable and multivariable logistic regression model was used to explore the associations between variants and hrHPV infection.Our results showed that hrHPV prevalence was 16.09% in Hubei Province,among which,hrHPV was more likely to be positive in women aged 51 years or above (OR=1.65,95% CI:1.28-2.14),and in women who had symptoms of bleeding after intercourse (OR=1.32,95% CI:1.17-1.50),had first sexual intercourse at the age of 18 years or below (OR=1.33,95% CI:1.07-1.64),had at least three male sexual partners (OR=2.50,95% CI:2.07-3.03),and who had been diagnosed with sexually transmitted infections (OR=1.50,95% CI:1.12-2.03).Married women (OR=0.66,95% CI:0.55-0.78) and women who frequently used condoms (OR=0.75,95% CI:0.67-0.84) had a relatively lower hrHPV prevalence.This study confirms that hrHPV infection was associated with age,marital status,symptoms of intercourse bleeding,history of sexually transmitted infections,and sex-related behaviors.Above all,this study provides a baseline database prior to obtaining vaccinations for dynamic tracking of the changes in hrHPV prevalence. 展开更多
关键词 high-risk human PAPILLOMAVIRUS CERVICAL cancer PREVALENCE risk FACTORS China
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Human papilloma virus and esophageal carcinoma in a Latin-American region 被引量:4
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作者 Roberto Herrera-Goepfert Marcela Lizano +2 位作者 Suminori Akiba Adela Carrillo-García Mauricio Becker-D'Acosta 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第25期3142-3147,共6页
AIM:To investigate the presence of high-risk human papilloma virus (HPV) in esophageal squamous cell carcinomas (ESCCs) in a non-selected Mexican population.METHODS: Cases with a pathological diagnosis of squamous cel... AIM:To investigate the presence of high-risk human papilloma virus (HPV) in esophageal squamous cell carcinomas (ESCCs) in a non-selected Mexican population.METHODS: Cases with a pathological diagnosis of squamous cell carcinoma of the esophagus were obtained from Department of Pathology files, at the National Cancer Institute in Mexico City during the period between 2000 and 2008. Slides from each case were reviewed and cases with sufficient neoplastic tissue were selected for molecular analysis. DNA was extracted from paraffin-embedded tissue samples for polymerase chain reaction analysis to detect HPV DNA sequences. Demographic and clinical data of each patient were retrieved from corresponding clinical records.RESULTS: HPV was detected in 15 (25%) of ESCCs. HPV-16 was the most frequently observed genotype, followed by HPV-18; HPV-59 was also detected in one case. Unfortunately, HPV genotype could not be established in three cases due to lack of material for direct sequencing, although universal primers detected the presence of HPV generic sequences. No low-risk HPV genotypes were found nor was HPV-16/18 co-infection. HPV presence in ESCC was not significantly associated with gender, age, alcohol consumption, smoking, anatomic location, or histologic grade. All patients belonged to low and very low socioeconomic strata, and were diagnosed at advanced disease stage. Male patients were most commonly affected and the male:female ratio in HPV-positive ESCC increased two- fold in comparison with HPV-negative cases (6.5:1 vs 3.1:1).CONCLUSION: High prevalence of high-risk HPV in ESCC in Mexico does not support the hypothesis that HPV-associated ESCC is more common in areas with higher ESCC incidence rates. 展开更多
关键词 ESOPHAGUS Squamous cell carcinoma virushuman papilloma virus high-risk human papilloma virus
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Human papillomavirus-associated diseases and cancers
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作者 Lan Yang Jianbo Zhu +3 位作者 Xiaoyue Song Yan Qi Xiaobin Cui Feng Li 《Oncology and Translational Medicine》 CAS 2015年第3期109-114,共6页
Human papillomaviruses(HPVs) have been detected in cervical cancer cells and skin papilloma cells, which have a variety of types, including low-risk and high-risk types. HPV genome replication requires the host cell&#... Human papillomaviruses(HPVs) have been detected in cervical cancer cells and skin papilloma cells, which have a variety of types, including low-risk and high-risk types. HPV genome replication requires the host cell's DNA synthesis machinery, and HPVs encode proteins that maintain differentiated epithelial cells in a replication-competent state. HPV types are tissue-specific and generally produce different types of lesions, either benign or malignant. This review examines different HPV types and their associated diseases and presents therapeutic options for the treatment of HPV-positive diseases. 展开更多
关键词 human papillomavirus (HPV) LOW-RISK high-risk CANCER
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Smoking and genital human papilloma virus infection in women attending cervical cancer screening in Greece
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作者 Kimon Chatzistamatiou Taxiarchis Katsamagas +7 位作者 Menelaos Zafrakas Konstantia Zachou Anastasia Orologa Fani Fitsiou Thomas Theodoridis Theocharis Konstantinidis Theodoros C Konstantinidis Theodoros Agorastos 《World Journal of Obstetrics and Gynecology》 2013年第3期53-61,共9页
AIM: To investigate whether smoking is associated with human papilloma virus(HPV) infection. METHODS: HPV infection is considered to be a neces-sary condition for cervical cancer development. The study population incl... AIM: To investigate whether smoking is associated with human papilloma virus(HPV) infection. METHODS: HPV infection is considered to be a neces-sary condition for cervical cancer development. The study population included 1291 women, aged 25-55 years, attending cervical cancer screening. All women had a Papanicolaou(Pap) test, with liquid-based cytology(Thinprep), an HPV-DNA test and an evaluation of smoking habits. The COBAS 4800 system was used for HPV-DNA testing, enabling identifi cation of the following high-risk HPV(hr HPV)-types: each of HPVs 16 and 18 separately, and HPVs 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68 as a cocktail. The evaluation of smoking habits was assessed using the smoking intensity index(SII), a variable formed as the product of cigarettes consumed per day by the days(years × 365) that a woman was a smoker, divided by 1000. RESULTS: There were 136 smokers among 238 women tested positive for hr HPV-types(HPVs 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and/or 68), and 463 smokers among 1053 hr HPV-negative women(OR = 1.7, P < 0.001). This association was attributed to the youngest age group of women, aged 25-34 years(OR = 2.3, P < 0.001), while there was no association in other age groups. The intensity of smoking(increasing SII) showed no statistically signifi cant association with hr HPV infection. Cervical infection with HPV 16 and/or HPV 18 was also not associated with age or smoking habits. Finally, no association was found between Pap test status and smoking habits or smoking intensity. CONCLUSION: Smoking appears to be associated with hr HPV infection of the uterine cervix, particularly in younger women. Further studies should investigate whether this association is based on causality and evaluate the role of other possible co-factors. 展开更多
关键词 human papilloma virus high-risk human papilloma virus human papilloma virus-DNA test Smok-ing Cervical cancer SCREENING
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Efficacy of 2LPAPI®, a Micro-Immunotherapy Drug, in Patients with High-Risk Papillomavirus Genital Infection
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作者 Gilles Thomas Hélène Cluzel +2 位作者 Jacqueline Lafon Jacques Bruhwyler Béatrice Lejeune 《Advances in Infectious Diseases》 2016年第1期7-14,共8页
Human papillomaviruses (HPVs) are well known for being linked to the development of cervical cancers, most of them being caused by the high-risk (HR) oncogenic genotypes, mainly 16 and 18. The efficacy of 2LPAPI<su... Human papillomaviruses (HPVs) are well known for being linked to the development of cervical cancers, most of them being caused by the high-risk (HR) oncogenic genotypes, mainly 16 and 18. The efficacy of 2LPAPI<sup><sup>&reg;</sup> </sup>(Labo’Life), a micro-immunotherapy homeopathic drug, has been evaluated in HR-HPV infected women (n = 18), in a private gynecology practice, by comparing them to an untreated control group (n = 18). Patients were 20 to 45 years old and had cytology with Atypical Squamous Cells of Undetermined Significance (ASCUS) or Low grade Superficial Intra Lesions/ Cervical Intraepithelial Neoplasia Grade I (LSIL/CINI). Patients freely chose to be treated with the drug or not. Those deciding not to take the drug remained untreated and were followed as a control group. The drug was taken at the regimen of one capsule per day during 6 months. HR-HPV and cytology were evaluated at 6 and 12 months. After 12 months, HR-HPV was cleared in 78% of the patients taking the drug versus 44% in those not taking it (p = 0.086). In patients over 25 years, HR-HPV clearance in the treated group was significantly higher (81.3%) than in the control group (20%) (p = 0.004). The difference in the regression of the lesion grades almost reached statistical significance (p = 0.053). This follow-up confirms that the micro-immunotherapy drug 2LPAPI<sup><sup>&reg;</sup></sup> is a safe and effective therapeutic approach to treat HR-HPV cervical lesions in women over 25 years. 展开更多
关键词 high-risk human Papillomavirus Micro-Immunotherapy Genital Infection HOMEOPATHY 2LPAPI®
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鲍温病HPV分型和hMLH1,hMSH2蛋白的检测 被引量:3
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作者 张跃 袁伟 贾常莎 《中国皮肤性病学杂志》 CAS 北大核心 2014年第1期11-14,共4页
目的探讨人乳头瘤病毒感染与不同部位鲍温病(Bowen)病发病的关系并检测肿瘤错配修复基因hMLH1,hMSH2蛋白在Bowen病中的表达情况。方法 20例Bowen病组织来源于2004-2011年皮肤科病理证实标本,其中肛周和外生殖器共3例,其他部位共17例,另... 目的探讨人乳头瘤病毒感染与不同部位鲍温病(Bowen)病发病的关系并检测肿瘤错配修复基因hMLH1,hMSH2蛋白在Bowen病中的表达情况。方法 20例Bowen病组织来源于2004-2011年皮肤科病理证实标本,其中肛周和外生殖器共3例,其他部位共17例,另取10例正常皮肤为对照。采用人乳头瘤病毒核酸扩增分型方法对Bowen病皮损进行基因分型检测。免疫组化法检测Bowen病组织及10例正常皮肤组织中hMLH1,hMSH2蛋白的表达。结果 20例Bowen病患者中肛周和外生殖器、其他部位共检出6例HPV阳性(30.00%),为HPV16,HPV18,HPV33型。Bowen病组织中hMLH1,hMSH2蛋白阳性表达较正常皮肤组织降低,差异有统计学意义(P<0.05);Bowen病高危型HPV感染组中hMLH1,hMSH2蛋白阳性表达低于非感染组(P<0.05)。结论 Bowen病的发生可能与HPV感染有关,Bowen病患者皮损中hMLH1,hMSH2蛋白低表达也许与其进展为肿瘤有关联。 展开更多
关键词 鲍温病 人乳头瘤病毒 HMLH1 HMSH2
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HPV生物蛋白敷料治疗宫颈癌前病变合并高危型HPV感染效果 被引量:3
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作者 郭垒 马艳 陈秀兰 《中国计划生育学杂志》 2023年第5期1047-1051,共5页
目的:比较保妇康栓、辛复宁与抗HPV生物蛋白敷料治疗宫颈癌前病变合并高危型人乳头瘤病毒(HR-HPV)感染的疗效。方法:选取2019年1月-2021年5月本院收治的宫颈癌前病变合并HR-HPV感染患者212例,随机分为4组各53例,保妇康栓组给予保妇康栓... 目的:比较保妇康栓、辛复宁与抗HPV生物蛋白敷料治疗宫颈癌前病变合并高危型人乳头瘤病毒(HR-HPV)感染的疗效。方法:选取2019年1月-2021年5月本院收治的宫颈癌前病变合并HR-HPV感染患者212例,随机分为4组各53例,保妇康栓组给予保妇康栓经阴道给药,辛复宁组给予辛复宁经阴道给药,抗HPV敷料组给予抗HPV生物蛋白敷料经阴道给药,对照组不予处置仅定期随访。比较各组HPV转阴率及不良反应。结果:停药后1、3、6、12个月,HPV转阴率和TCT检查结果有效率保妇康栓(42.9%)、辛复宁(47.9%)与抗HPV敷料组(66.7%)均高于对照组(9.8%),且抗HPV敷料组高于保妇康栓组和辛复宁组(均P<0.05),但保妇康栓组与辛复宁组无差异(P>0.05)。阴道镜检查逆转率,保妇康栓(18.2%)、辛复宁(20.0%)与抗HPV敷料组(35.3%)高于对照组(0),且抗HPV敷料组逆转率高于保妇康栓组和辛复宁组(均P<0.05)。各组均未发生严重不良反应。结论:保妇康栓、辛复宁与抗HPV生物蛋白敷料治疗宫颈癌前病变合并HR-HPV感染均有一定疗效,抗HPV生物蛋白敷料疗效优于保妇康栓和辛复宁。 展开更多
关键词 宫颈癌前病变 高危型人乳头瘤病毒 抗HPV生物蛋白敷料 保妇康栓 辛复宁 临床疗效
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Clinical significance of extended high-risk human papillomavirus genotyping and viral load in cervical cancer and precancerous lesions 被引量:2
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作者 Pingping Su Jincheng Ma +2 位作者 Lirui Yu Shuting Tang Pengming Sun 《Gynecology and Obstetrics Clinical Medicine》 2023年第1期22-29,共8页
Persistent infections with specific high-risk human papillomavirus(HR-HPV)strains are the leading cause of cervical cancer and precancerous lesions.HPV-16 and HPV-18 are associated with more than 70%of cervical cancer... Persistent infections with specific high-risk human papillomavirus(HR-HPV)strains are the leading cause of cervical cancer and precancerous lesions.HPV-16 and HPV-18 are associated with more than 70%of cervical cancer.However,with recent widespread vaccination efforts against cervical cancer,the infection rates of HPV-16 and HPV-18 have decreased across all age groups,while the infection rates of other HR-HPV strains have increased.The non-16/18 HR-HPV strains play an important role in cervical lesions.These strains can be identified with extended genotyping,and the 2019 American Society for Colposcopy and Cervical Pathology(ASCCP)guidelines recommended an HPV-based testing to assess the risk of cervical disease in patients.We reviewed and analyzed the clinical benefits of applying extended HR-HPV genotyping,which was published by the International Agency for Research on Cancer(HPV-16,18,31,33,35,39,45,51,52,56,58,59,66,and 68),to cervical cancer screening.This review concluded that cervical cancer screening needs to include extended HR-HPV genotyping.The examination of extended HR-HPV genotyping in cervical intraepithelial lesions and cervical cancers can help guide clinical practices. 展开更多
关键词 high-risk human papillomavirus Cervical cancer GENOTYPE Viral load Cancer screening
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High-risk human papilloma virus infection and cervical neoplasm in female inflammatory bowel disease patients:a cross-sectional study 被引量:1
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作者 Miao Li Qing-Fan Yang +15 位作者 Qian Cao Jian Tang Yu Gao Min Zhi Kang Chao Ming-Li Su Wei-Min Huang Yan Yi Sui-Yan Xia Ling-Jie Huang Yuan Zhao Xiao-Hong Wang Xiao-Ying Liu Lin Lin Pin-Jin Hu Xiang Gao 《Gastroenterology Report》 SCIE EI 2019年第5期338-344,I0002,共8页
Background and aim:This cross-sectional study investigated the prevalence and risk factors of high-risk human papilloma virus(HPV)infection,especially types 16 and 18,and cervical neoplasia in female Inflammatory bowe... Background and aim:This cross-sectional study investigated the prevalence and risk factors of high-risk human papilloma virus(HPV)infection,especially types 16 and 18,and cervical neoplasia in female Inflammatory bowel disease(IBD)patients.Methods:From July 2014 to January 2017,sexually active,female,Chinese IBD patients(21–60 years)and age-matched controls underwent cervical ThinPrep cytology testing(TCT)and high-risk HPV-DNA detection,and completed questionnaires about awareness of cervical cancer and HPV.Cervical dysplasia was categorized as cervical intraepithelial neoplasia(CIN)1,2 and 3.Results:Of 124 IBD patients(30 ulcerative colitis and 94 Crohn’s disease),17(13.7%)had high-risk HPV among whom 9(7.3%)had HPV 16/18 infection and 4(3.2%)had cervical CIN(3 CIN 3,1 CIN 1)by pathology.Among 372 controls,33(8.9%)had high-risk HPV and only 1(0.3%)had HPV 16 infection.Cervical TCT detected atypical squamous cells of unknown significance in one control;no control had CIN.The HPV 16/18 infection rate and CIN prevalence were significantly higher in IBD patients than controls(both P<0.001).The HPV-infection rate was higher in patients administered methotrexate[P=0.005,odds ratio(95%confidence interval)4.76(1.471–15.402)]or more than two immunosuppressants[P=0.013,odds ratio(95%confidence interval)3.64(1.255–10.562)].Thiopurine,steroid,infliximab and disease behavior/location were not associated with HPV infection.Only 29.3%of patients had undergone cervical-cancer screening.Awareness of HPV infection and HPVrelated cervical cancer was poor(28.2%).Conclusions:Female IBD patients are at increased risk of high-risk HPV infection and cervical neoplasia,which may be associated with immunosuppressants.Education and routine follow-up with HPV-DNA testing and TCT are recommended,especially in female Chinese IBD patients. 展开更多
关键词 Inflammatory bowel disease high-risk human papilloma virus cervical intraepithelial neoplasia
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Evaluation of dry specimen transport and processing time using an isothermal amplification high-risk human papillomavirus assay
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作者 Longhua Hu Suhui Wu +5 位作者 Haixia Shang Jerome Belinson Jinhong Li Xiaoqin Wu Yaling Huang Jingfen Sun 《Gynecology and Obstetrics Clinical Medicine》 2022年第1期34-37,共4页
Background:Dry specimen transport has shown equivalence to traditional liquid transport using a novel high-risk Human papillomavirus assay.Considering that dry transport might cross obstacles during cervical cancer sc... Background:Dry specimen transport has shown equivalence to traditional liquid transport using a novel high-risk Human papillomavirus assay.Considering that dry transport might cross obstacles during cervical cancer screening in low and middle resource settings,this study was designed evaluate different processing time of dry specimen transport using the same isothermal amplification hrHPV assay.Methods:There were 564 women between the ages of 30–55 recruited from colposcopy clinic.For each patient,two endocervical samples were collected and placed into empty collection tubes by physician.Samples were stored at room temperature until analyzed for hrHPV using the AmpFire assay at two time points:2 days and 2 weeks.511 of the 564 participants with positive hrHPV were provided colposcopy exam and quadrant biopsy.Results:A total of 1128 endocervical samples from 564 patients were detected by the Ampfire assay.Good agreement was found between two time periods(KappaStandard error=0.67±0.04).Sensitivity(2days/2weeks)for CIN2t was 95.28%(95%CI:92.14%–98.42%)vs 90.57%(CI(86.65%–94.49%)and specificity(2days/2weeks)was 22.47%(CI 19.33%–25.61%)vs 28.15%(CI 24.23%–32.07%)respectively.The difference for Ampfire HPV detection in sensitivity for CIN2t for the two time periods was not significant(P=0.227),while the difference in specificity for CIN2t was significant(P=0.001).The difference in Ct values 29.23(CI 28.15–30.31)and 29.27(CI 28.19–30.35)between two time points was not significant(P?0.164).Conclusion:Processing dry brush specimens can be delayed up to 2 weeks.Using the AmpFire assay platform which supports cervical cancer prevention programs in low-to-middle-income countries(LMICs). 展开更多
关键词 Cervical cancer SCREENING high-risk human papillomavirus Specimen transport Processing time Sample preparation
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SCREENING FOR A 21-CHROMOSOME ABNORMALITY IN PREIMPLANTED EMBRYOS OF ELDERLY WOMEN
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作者 Fang-YinMeng Xiao-hongLi 《Chinese Medical Sciences Journal》 CAS CSCD 2004年第4期285-285,共1页
关键词 Chromosome Aberrations Chromosomes human Pair 21 Fertilization in Vitro Preimplantation Diagnosis ADULT Down Syndrome FEMALE humans In Situ Hybridization Fluorescence Maternal Age PREGNANCY Pregnancy high-risk Research Support Non-U.S. Gov't
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Correlation of HPV infection with TLRs pathway activation and downstream gene expression in cervical precancerous lesions
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作者 Bilikezi·aikemu Wei Gong +1 位作者 Bahargul·ismawyil Ming-Ming Yue 《Journal of Hainan Medical University》 2018年第12期63-66,共4页
Objective: To study the correlation of HPV infection with TLRs pathway activation and downstream gene expression in cervical precancerous lesions. Methods: Cervical precancerous lesion tissues from surgical resection ... Objective: To study the correlation of HPV infection with TLRs pathway activation and downstream gene expression in cervical precancerous lesions. Methods: Cervical precancerous lesion tissues from surgical resection or cone biopsy in Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University between June 2014 and May 2017 were collected and divided into HR-HPV positive group and HR-HPV negative group according to the condition of high-risk HPV infection;normal cervical tissues surgically removed due to fibroid in Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University during the same period were collected as the control group. The contents of TLRs pathway molecules as well as the expression of apoptosis-related genes and invasion-related genes in the cervical precancerous lesion tissues and normal cervical tissues were determined. Results:TLR2, TLR3, TLR4, TLR9 and NF-κB contents as well as CyclinD1, PCNA, iNOS, Gal-9, N-cadherin, HIF-1α and VEGF mRNA expression in HR-HPV positive group and HR-HPV negative group were significantly higher than those in control group whereas p53, Beclin1, LC3-II and SFRP2 mRNA expression were significantly lower than those in control group, and TLR2, TLR3, TLR4, TLR9 and NF-κB contents as well as CyclinD1, PCNA, iNOS, Gal-9, N-cadherin, HIF-1α and VEGF mRNA expression in HR-HPV positive group were significantly higher than those in HR-HPV negative group whereas p53, Beclin1, LC3-II and SFRP2 mRNA expression were significantly lower than those in HR-HPV negative group. Conclusion: HPV infection in cervical precancerous lesions can regulate downstream apoptosis and invasion gene expression by activating multiple TLRs pathways. 展开更多
关键词 Cervical cancer Precancerous lesion high-risk human papillomavirus Toll-like receptor
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Relevant Frequency of Multiple Infections with High- and Low-Risk HPV Genotypes among Mexican Women Attending a Tertiary Care Hospital
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作者 Saúl Flores-Medina Carmen S. García-Romero +3 位作者 Diana M. Soriano-Becerril Francisco J. Díaz-García Silvia Giono-Cerezo Graciela Castro-Escarpulli 《Open Journal of Obstetrics and Gynecology》 2016年第7期424-432,共9页
Aims: To assess the frequency and the main HPV genotypes circulating among a group of women attending at a third level Hospital in Mexico City. Methods: A cross-sectional and descriptive study was performed in a group... Aims: To assess the frequency and the main HPV genotypes circulating among a group of women attending at a third level Hospital in Mexico City. Methods: A cross-sectional and descriptive study was performed in a group of 143 female outpatients of the Gynecology and Obstetrics Service at the National Institute of Perinatology of Mexico. Cervical swabs were taken from participants and subjected to simultaneous detection/genotyping of HPV by Linear Array Genotyping Test (Roche Molecular Systems). Mann-Whitney U, median and/or Square Chi tests were used to compare socio-demographical features between HPV-infected and uninfected women. Results: A total of 66 women (46.2%) had HPV infection. Overall, 112 genotypes were detected either as single infections (45.5%) or multiple genotype infections (54.5%). The cumulated frequency of multiple infections with high-/low- and high-/high-risk HPV genotypes was 63.9 %. The most frequent high-risk genotypes were HPV52 HPV58 and HPV51, whereas the most frequent low-risk genotypes were HPV6, HPV53 and HPV84. Infected women were significantly younger and have less stable partner relationships than uninfected women (p < 0.05). Conclusion: A relevant frequency of mixed infections with high- and low-risk HPV genotypes, other than those considered most prevalent worldwide, was observed. Most circulating high-risk genotypes among the women of this study are not covered by commercial vaccine formulations. 展开更多
关键词 human Papillomavirus high-risk Genotypes HPV Co-Infections Linear Array Genotyping
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Photodynamic Therapy for Low-grade Cervical Intraepithelial Neoplasia(CIN1):A Case Report
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作者 Yinyin Zhang Hong Lin Huizhen Fan 《Journal of Clinical and Nursing Research》 2020年第6期27-31,共5页
As a drug-mechanical combination technology,photodynamic(PDT)can achieve accurate and taurgeted therapy for maligmant tumors and benign diseases through the production of reactive oxygen species,oxygen free radicals o... As a drug-mechanical combination technology,photodynamic(PDT)can achieve accurate and taurgeted therapy for maligmant tumors and benign diseases through the production of reactive oxygen species,oxygen free radicals or singlet oxygen by photosensitizers at specific wavelengths.Compared with traditional surgery,it has the advantages of selective killing.repeatable teatment,preserving target organ fiunction and so on.The purpose of this study was to explore the clinical value of photodynamic therapy in cervical precancerous lesions by taking the patients with low-grade cervical intaepithelial neoplasia(CIN1)with high-risk human papillomavinus(HR HPV)persistent infection diagnosed by"three-step diagnosis and teatment procedure"as an example.Using HiPorfn as a photosensitizer,photodynamic therapy was performed 48 hours after intavenous drip.Set laser wavelength 630nm,light dose density 137.58J/cm^2,ansmission efficiency 1.42,output power 2w.3cm columnar optical fber was placed around the 2cm in the cervical canal to cover all the lesions,and the inadiation time was 900s(600s in the cervical canal and 300s outside the cervical canal).The patients were given oxygen inhalation for 6 hours after operation,and the patients were observed for itching and other discomfort,and paid attention to avoid light.Photodynamic therapy was performed again in the same way on the second day.After two months of treatment,pathological biopsy showed chronic cervicitis,indicating that the disease had been effectively controlled.Theoretically,although the patient is not the absolhute indication of photodynamic therapy(hat is,meeting CIN Ⅱ or CIN Ⅲ,having fertility requirements and not undergoing surgery),this therapy can remove not only the superficial lesions inside and outside the cervix,but also the potential lesions not found under colposcopy.It can also block the persistent infection of HPV by.inhbting the expression of HPV18,E6 and E7mRNA in Hela cells.In combination with Baofukang suppository,it can block HPV infection.Increase the negative conversion rate of cervical HPV and reduce the probablity of recurence after CIN1 cure.For youmng female patients with persistent HR-HPV infection and fertility requirements,photodynamic therapy is an effective choice for clinical treatment of CIN1. 展开更多
关键词 Photodynamic HiPorfin Low-grade cervical intraepithelial neoplasia high-risk human papillomavinus
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HPV基因分型及p16/ki-67双染色对宫颈转化区3型女性CINⅡ+的评估价值
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作者 高月月 顾丽霞 +3 位作者 杨梦霞 朱慧 冯海娇 陈书玲 《中国计划生育和妇产科》 2024年第8期85-89,共5页
目的分析人乳头瘤病毒(hunan papillomavirus,HPV)检测和p16/ki-67双染对宫颈转化区(TZ)3型女性宫颈上皮内瘤变(CIN)Ⅱ及以上病变的诊断价值。方法前瞻性收集2020年1月至2022年12月在河北北方学院附属第一医院接受阴道镜检查和宫颈移行... 目的分析人乳头瘤病毒(hunan papillomavirus,HPV)检测和p16/ki-67双染对宫颈转化区(TZ)3型女性宫颈上皮内瘤变(CIN)Ⅱ及以上病变的诊断价值。方法前瞻性收集2020年1月至2022年12月在河北北方学院附属第一医院接受阴道镜检查和宫颈移行区大环切除术(LLETZ)治疗的TZ 3型女性临床资料。分析并比较LLETZ前宫颈液基细胞学检查(TCT)、HPV检测、宫颈管搔刮术(ECC)检查及p16/ki-67双染对CINⅡ+的诊断价值。分别计算不同诊断方式预测组织学诊断CINⅡ+的敏感性、特异性、阳性预测值和阴性预测值。并使用McNemar检验比较不同诊断方法。结果纳入接受LLETZ治疗的患者共101例,其中CINⅡ+51例(50.5%)。37例HSIL患者中28例CINⅡ+(75.7%);64例LSIL患者中23例CINⅡ+(35.9%)。感染HPV 16型女性中82.1%组织病理学为CINⅡ+,但CINⅡ+患者中感染HPV 16型女性仅为45.1%。高危型HPV预测诊断CINⅡ+的阳性预测值仅为60.7%,阴性预测值为100%。p16/ki-67双染检查的阴性预测值为100%,阳性预测值为79.7%。LSIL女性中p16/ki-67双染预测CINⅡ+的阳性预测值较高危型HPV更高(76.1%vs.35.9%),而在HSIL女性中ECC双染可改善高危型HPV阳性预测值(96.5%vs.75.7%)。结论p16/ki-67双染可提高TZ 3型女性常规筛查对CIN II+预测效能,有助于更好监测TZ 3型女性宫颈情况。 展开更多
关键词 宫颈癌 人乳头瘤病毒 P16 KI-67 病理学
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阴道镜检查对宫颈癌前病变进展的预测作用进展
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作者 张丽 《首都食品与医药》 2024年第20期11-14,共4页
宫颈癌前病变(Cervical intraepithelial neoplasia,CIN)是宫颈癌发展过程中的一个阶段.通过早期筛查和预防措施,宫颈癌前病变可以得到有效控制和治疗.阴道镜检查是宫颈癌前病变管理中不可或缺的工具,它有助于提高早期诊断率,优化治疗方... 宫颈癌前病变(Cervical intraepithelial neoplasia,CIN)是宫颈癌发展过程中的一个阶段.通过早期筛查和预防措施,宫颈癌前病变可以得到有效控制和治疗.阴道镜检查是宫颈癌前病变管理中不可或缺的工具,它有助于提高早期诊断率,优化治疗方案,并为宫颈癌的预防和控制提供重要信息.此文首先概述了宫颈癌前病变的发病现状,总结了宫颈癌前病变的常规检查方法——人乳头瘤病毒(Human papillomavirus,HPV)检查、液基细胞学检测(Thinprep cytologic test,TCT)、活检、影像学检查,明确了阴道镜检查对宫颈癌前病变进展的预测作用进展,并阐述了阴道镜检查对宫颈癌前病变进展预测作用的局限性. 展开更多
关键词 阴道镜 宫颈癌前病变 预测作用 液基细胞学检测 人乳头瘤病毒 影像学检查
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基于16SrDNA高通量测序探究HPV52感染对女性阴道微生态的影响 被引量:6
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作者 祝江红 黄蕾 韦妹艳 《中国病原生物学杂志》 CSCD 北大核心 2023年第9期1005-1011,共7页
目的 基于16SrDNA高通量测序探究HPV52感染对女性阴道微生态的影响。方法 选取2022年4~12月长沙市第一医院妇科、九江市第三人民医院妇科收治的女性患者142例,进行阴道菌群检测和HPV52检测,分析阴道菌群特征与HPV52感染之间的关系。结果... 目的 基于16SrDNA高通量测序探究HPV52感染对女性阴道微生态的影响。方法 选取2022年4~12月长沙市第一医院妇科、九江市第三人民医院妇科收治的女性患者142例,进行阴道菌群检测和HPV52检测,分析阴道菌群特征与HPV52感染之间的关系。结果 共检出阴道菌门28个,阴道菌属453个,其中主要菌门为厚壁菌门,其它依次为放线菌门、拟杆菌门等。主要菌属为乳杆菌属,其它依次为加德纳菌属、嗜胨菌属等。经测序共获得8 689个OTU,其中HPV52感染组特有1 768个,HPV阴性组特有2 114个,2组共有4 807个。HPV52阳性组ACE指数、Shannon指数以及Chaol指数均稍高于HPV52阴性组,Simpson指数稍低于HPV52阴性组。门水平以及属水平HPV52阳性患者与HPV52阴性患者菌群分布存在一定差异,网络图直观反应HPV52阳性患者与HPV52阴性患者阴道菌群分布情况。结论 HPV52阳性以及HPV52阴性患者主要感染菌门均为厚壁菌门,均以乳杆菌属为优势菌,HPV52阳性组患者阴道菌群多样性高于HPV52阴性患者,HPV52感染可能与嗜胨菌属、纤毛菌属等水平升高有关,因此可从维持阴道菌群平衡角度预防和治疗HPV52感染所致的相关疾病。 展开更多
关键词 阴道菌群 人乳头瘤病毒52 微生态 高通量测序
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宫颈癌前病变及宫颈癌与人乳头状瘤病毒感染 被引量:1
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作者 李晓娟 《中国卫生检验杂志》 CAS 2023年第7期853-857,共5页
目的检测分析宫颈癌前病变及宫颈癌人乳头状瘤病毒(HPV)感染类型。方法回顾性选取2020年2月—2022年6月本院HPV感染检测患者2042例,依据检测结果分为宫颈癌组(1164例)、宫颈癌前病变组(444例)、宫颈细胞学正常组(434例)3组。统计分析3... 目的检测分析宫颈癌前病变及宫颈癌人乳头状瘤病毒(HPV)感染类型。方法回顾性选取2020年2月—2022年6月本院HPV感染检测患者2042例,依据检测结果分为宫颈癌组(1164例)、宫颈癌前病变组(444例)、宫颈细胞学正常组(434例)3组。统计分析3组一般资料、HPV感染类型,并统计分析3组不同年龄、民族患者的HPV感染情况,分析3组主要感染类型分布情况,统计分析宫颈癌组不同病理特征患者的HPV感染类型。结果宫颈癌组患者年龄≤35岁比率低于宫颈癌前病变组、宫颈细胞学正常组,>35岁比率高于宫颈癌前病变组、宫颈细胞学正常组(P<0.05)。宫颈癌组、宫颈癌前病变组患者的HPV感染率均高于宫颈细胞学正常组(P<0.05),其中宫颈癌组患者的HPV多重感染率低于宫颈癌前病变组(P<0.05),高于宫颈细胞学正常组(P<0.05)。宫颈癌组年龄>35岁患者的HPV感染率高于≤35岁患者(P<0.05),宫颈细胞学正常组年龄>35岁患者的HPV感染率低于≤35岁患者(P<0.05)。宫颈癌组患者的主要感染类型为HPV16、HPV18、HPV58、HPV52、HPV33,宫颈癌前病变组患者的主要感染类型为HPV16、HPV58、HPV33、HPV52,宫颈细胞学正常组患者的主要感染类型为HPV16、HPV58、HPV18、HPV52。宫颈癌组鳞状细胞癌患者的HPV感染率高于非鳞状细胞癌患者(P<0.05),其中高分化患者的单一感染率低于低分化、中分化患者,多重感染率高于低分化、中分化患者(P<0.05)。结论宫颈癌HPV感染率高于宫颈癌前病变,类型主要为多重感染。 展开更多
关键词 宫颈癌前病变 宫颈癌 人乳头状瘤病毒 感染类型 病理特征
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