High-temperature stress suppresses allene oxide cyclase 2 and causes male sterility in cotton by disrupting jasmonic acid signaling

Cotton(Gossypium spp.) yield is reduced by stress. In this study, high temperature(HT) suppressed the expression of the jasmonic acid(JA) biosynthesis gene allene oxide cyclase 2(GhAOC2), reducing JA content and causing male sterility in the cotton HT-sensitive line H05. Anther sterility was reversed by exogenous application of methyl jasmonate(MeJA) to early buds. To elucidate the role of GhAOC2 in JA biosynthesis and identify its putative contribution to the anther response to HT, we created gene knockout cotton plants using the CRISPR/Cas9 system. Ghaoc2 mutant lines showed male-sterile flowers with reduced JA content in the anthers at the tetrad stage(TS), tapetum degradation stage(TDS), and anther dehiscence stage(ADS). Exogenous application of MeJA to early mutant buds(containing TS or TDS anthers) rescued the sterile pollen and indehiscent anther phenotypes, while ROS signals were reduced in ADS anthers. We propose that HT downregulates the expression of GhAOC2 in anthers, reducing JA biosynthesis and causing excessive ROS accumulation in anthers, leading to male sterility. These findings suggest exogenous JA application as a strategy for increasing male fertility in cotton under HT.

Osmotic Regulation,Antioxidant Enzyme Activities and Photosynthetic Characteristics of Tree Peony(Paeonia suffruticosa Andr.)in Response to High-Temperature Stress

Tree peony(Paeonia suffruticosa Andr.)is a traditional Chinese flower,which prefers cool weather.However,high temperature in summer in the middle and lower reaches of the Yangtze River restricts its growth and development.In this study,osmotic regulation,antioxidant enzyme activities,and photosynthetic characteristics of tree peony in response to high-temperature stress were investigated.The results showed that high-temperature stress had destroyed the cell membrane,manifested as the increased relative electrical conductivity and malondialdehyde content.Moreover,high-temperature stress led to excessive accumulation of reactive oxygen species,thereby,activating antioxidant enzyme activities.Also,photosynthetic parameters and chlorophyll fluorescence parameters directly reflected the damage to the photosystem II reflection center under high-temperature stress.In addition,high-temperature stress led to stomatal closure and chloroplast damage.This study revealed the physiological responses of tree peony to high-temperature stress,laying a foundation for the promotion of tree peony in high-temperature areas and the improvement of high-temperature resistance.

Analysis on high-temperature oxidation and growth stress of iron-based alloy using phase field method

High-temperature oxidation is an important property to evaluate thermal protection materials. However, since oxidation is a complex process involving microstructure evolution, its quantitative analysis has always been a challenge. In this work, a phase field method （PFM） based on the thermodynamics theory is developed to simulate the oxidation behavior and oxidation induced growth stress. It involves microstructure evolution and solves the problem of quantitatively computational analysis for the oxidation behavior and growth stress. Employing this method, the diffusion process, oxidation performance, and stress evolution axe predicted for Fe-Cr-A1-Y alloys. The numerical results agree well with the experimental data. The linear relationship between the maximum growth stress and the environment oxygen concentration is found. PFM provides a powerful tool to investigate high-temperature oxidation in complex environments.

A thermal stress loading technique for large-sized hot dry rock mechanical tests

Testing of large-sized specimens is becoming increasingly important in deep underground rock mechanics and engineering.In traditional mechanical loading,stresses on large-sized specimens are achieved by large host frames and hydraulic pumps,which could lead to great investment.Low-cost testing machines clearly always have great appeal.In this study,a new approach is proposed using thermal expansion stress to load rock specimens,which may be particularly suitable for tests of deep hot dry rock with high temperatures.This is a different technical route from traditional mechanical loading through hydraulic pressure.For the rock mechanics test system of hot dry rock that already has an investment in heating systems,this technology may reduce the cost of the loading subsystem by fully utilizing the temperature changes.This paper presents the basic principle and a typical design of this technical solution.Preliminary feasibility analysis is then conducted based on numerical simulations.Although some technical details still need to be resolved,the feasibility of this loading approach has been preliminarily confirmed.

Physiological Response of Spring Soybean Seedlings Under High-temperature Stress

To understand the response characteristics of soybean seedling resistance systems to high-temperature stress,the spring soybean varieties HN44 and HN65 were selected in this study as the experimental materials,as they had substantial differences in stress resistance.Soybean physiological status was studied under high-temperature stress.Sand culture was used in the experiment,and soybeans at the seedling stage were treated with high-temperature stress.The results showed that the activity of antioxidases and osmotic regulatory substance contents in soybean increased under high-temperature treatment for a certain period of time,the activity of antioxidases and osmotic regulatory substance contents decreased after continuous treatment,and high temperatures continuously increased malondialdehyde contents in the two varieties.At high temperatures,the antioxidant activity and osmotic regulator contents of HN44 were higher than those of HN65,and the malondialdehyde contents of HN44 were higher than those of HN65.The above results showed that HN44 soybeans exhibited significantly higher resistance to high-temperature stress than HN65 variety.

The Effect of High Temperature after Anthesis on Rice Quality and Starch Granule Structure of Endosperm

To ascertain the effect mechanism of high temperature after anthesis on rice quality, the experiment was conducted with two rice lines, the heat-tolerant line 996 and heat-sensitive line 4628, with high temperature and optimal temperature in the growth chamber to investigate the effect of high temperature stress after anthesis on rice appearance quality, milling quality, cooking and eating quality and starch granule structure of endo- sperm. The result showed that milled rice rate, head rice rate, amylose content and gel consistency of both lines decreased under high temperature stress after anthesis, while the ratio of grain length to width, chalky rate, chalkiness, protein content increased. Under high temperature treatments, gelatinization temperature, final viscosity, set back and peak time increased, breakdown decreased, Mg content and K content increased, Mg/K ra- tio decreased. Under same treatment, the extent of rice quality of heat tolerant line 996 affected by high temperature was lower than that of heat sensitive line 4628. Under high temperature stress after anthesis, starch granule arranged untightly, most of starch granules existed in the form of a single starch endosperm, refractive index decreased, transparency reduced, and led to the formation of chalk. Under high temperature stress af- ter anthesis, the increase of protein content, the decrease of Mg/K, the changes of rice RVA profile characteristics and starch granule structure of endosperm could be the main reason for the decrease of rice cooking and eating quality and appearance quality.

Graphene/carbon structured catalyst layer to enhance the performance and durability of the high-temperature proton exchange membrane fuel cells

In this study,nitrogen doped electrochemically exfoliated reduced graphene oxide and carbon black supported platinum(Pt/Nr EGO_(2)-CB_(3))has been prepared to enhance the performance and durability of hightemperature PEMFCs with lower Pt loading.On the one hand,Pt/Nr EGO_(2)-CB_(3)with the strong interaction between the Pt and nitrogen(N)prevent agglomeration of Pt particles and Pt particles is 5.46±1.46 nm,which is smaller than that of 6.78±1.34 nm in Pt/C.Meanwhile,ECSA of Pt/Nr EGO_(2)-CB_(3)decrease 13.65%after AST,which is much lower than that of 97.99%in Pt/C.On the other hand,the Nr EGO flakes in MEAac act as a barrier to mitigate phosphoric acid redistribution,which improves the formation of triple-phase boundaries(TPBs)and gives stable operation of the MEAacwith a lower decay rate of 0.02 mV h^(-1)within100 h.After steady-state operation,the maximum power density of Pt/Nr EGO_(2)-CB_(3)(0.411 W cm^(-2))is three times higher than that of conventional Pt/C(0.134 W cm^(-2))in high-temperature PEMFCs.After AST,the mass transfer resistance of Pt/Nr EGO_(2)-CB_(3)electrode(0.560Ωcm^(2))is lower than that in Pt/C(0.728Ωcm^(2)).

水稻开花期高温热害响应机理及其调控技术研究进展

近年来,频繁发生的极端高温天气严重影响了水稻的生长发育、产量及稻米品质形成。水稻开花期对高温最为敏感,研究花期高温诱导小穗败育机理及其调控措施对减缓水稻高温热害,保障我国粮食安全生产具有重要意义。本文综述了高温下水稻颖花开放、花药开裂及散粉、柱头花粉粒萌发及花粉管伸长的特征及其作用机制,探究水稻花期耐热性调控机制以及减缓水稻花期高温热害的栽培调控措施,不仅为水稻花期高温热害机理及防控技术研究提供新的研究思路,还对今后的研究方向进行了展望。

外源L-谷氨酸对花后干旱胁迫下小麦干物质积累分配、籽粒灌浆特性及品质形成的调控效应

【目的】谷氨酸(Glu)具有调节植物生长,提高作物抗逆能力的功效。研究外源L-谷氨酸(L-Glu)对灌浆期干旱胁迫下小麦干物质积累分配及转运、光合参数、灌浆特性、农艺性状及品质特性的影响,为进一步研究谷氨酸提高小麦耐旱性奠定理论基础。【方法】以新疆主栽冬小麦品种新冬18号和新冬22号为试验材料,在抽穗期、开花期分别喷施0、2、4 mmol/L的L-Glu溶液,开花后进行大田干旱胁迫(DT)和适水处理(WT)。在开花初期和花后10、20、30天(即灌浆初期、中期、后期),测定了小麦干物质积累分配及转运、光合特性、灌浆特性、农艺性状及品质等相关指标。【结果】外源喷施L-Glu可以显著增加开花后干旱胁迫下地上部总干物质积累量,其中DT-G2、DT-G4处理显著高于DT-G0处理。与DT-G0处理相比,DT-G2和DT-G4处理有效提高了开花后干旱胁迫下干物质积累对籽粒干物质积累的贡献率,新冬18号小麦分别增加24.65%、24.28%,新冬22号小麦分别增加28.44%、16.03%;与DT-G0处理相比,DT-G2、DT-G4处理提高了灌浆期光合速率(P_(n))、气孔导度(G_(s))、蒸腾速率(T_(r))、叶肉瞬时羧化效率(P_(n)/C_(i))及籽粒灌浆平均速率,其中DT-G2、DT-G4处理下新冬18号小麦籽粒灌浆平均速率分别较DT-G0增加15.17%和3.59%,新冬22号小麦分别增加19.92%和14.70%;与DT-G0处理相比,DT-G2处理显著提高了2个小麦品种的千粒重,DT-G4处理显著降低了2个小麦品种的湿面筋含量。【结论】抽穗期、开花期喷施外源L-Glu可以显著增加开花后干旱胁迫下小麦地上部总干物质积累量,降低灌浆后期小麦干物质在营养器官的分配比例,同时提高开花后干旱胁迫下旗叶的光合速率及蒸腾速率,进而促进小麦穗部干物质的分配,提高小麦籽粒的灌浆速率,延长灌浆持续时间,最终在降低干旱胁迫下粒重损失的同时提高籽粒品质。

Rice Heat Tolerance Breeding: A Comprehensive Review and Forward Gaze

The yield potential of rice is seriously affected by heat stress due to climate change. Since rice is a staple food globally, it is imperative to develop heat-resistant rice varieties. Thus, a thorough understanding of the complex molecular mechanisms underlying heat tolerance and the impact of high temperatures on various critical stages of the crop is needed. Adoption of both conventional and innovative breeding strategies offers a long-term advantage over other methods, such as agronomic practices, to counter heat stress. In this review, we summarize the effects of heat stress, regulatory pathways for heat tolerance, phenotyping strategies, and various breeding methods available for developing heat-tolerant rice. We offer perspectives and knowledge to guide future research endeavors aimed at enhancing the ability of rice to withstand heat stress and ultimately benefit humanity.

藜麦开花期干旱胁迫对其形态特征及生理特性的影响

为探究干旱胁迫对开花期藜麦形态特征的影响,以陇藜1号、青藜1号两个藜麦品种为试验材料,以正常灌溉为对照,设置3个干旱胁迫强度处理,在藜麦开花期进行干旱胁迫15 d后,测定各处理植株叶片相对含水量、叶比重、过氧化氢酶(CAT)活性、丙二醛(MDA)含量等生理指标,干旱胁迫15 d后恢复正常灌水,成熟时测定植株高度、主穗长和有效分枝数。结果表明,植株叶片相对含水量和叶比重随土壤水分的降低而下降,不同程度干旱胁迫处理对叶片相对含水量影响差异不显著,但对叶比重影响显著;MDA含量、CAT活性也与干旱胁迫程度呈正相关,即随干旱胁迫强度的加重而上升。干旱胁迫处理后,植株高度、主穗长和有效分枝数下降,但轻度和中度干旱胁迫对株高、主穗长度影响差异不显著,重度干旱胁迫显著影响株高和主穗长,中度和重度干旱胁迫显著影响有效分枝数。说明开花期适度干旱胁迫对藜麦形态特征影响不大。

The effects of sulfide stress cracking on the mechanical properties and intergranular cracking of P110 casing steel in sour environments

Variation and degradation of P-110 casing steel mechanical properties, due to sulfide stress cracking （SSC） in sour environments, was investigated using tensile and impact tests. These tests were carried out on specimens, which were pretreated under the following conditions for 168 hours： temperature, 60 ℃; pressure, 10 MPa; H2S partial pressure, 1 MPa and CO2 partial pressure, 1 MPa; preload stress, 80% of the yield strength （os）; medium, simulated formation water. The reduction in tensile and impact strengths for P-110 casing specimens in corrosive environments were 28% and 54%, respectively. The surface morphology analysis indicated that surface damage and uniform plastic deformation occurred as a result of strain aging. Impact toughness of the casing decreased significantly and intergranular cracking occurred when specimens were maintained at a high stress level of 85% %.

不同玉米种质花期对干旱和高温胁迫的响应

【目的】探讨不同玉米种质农艺性状和光合生理性状对干旱和高温胁迫的响应,以期为玉米耐旱和耐热种质的筛选及热带种质的利用提供理论依据。【方法】以热带种质、温带种质和温热种质各25份为试验材料,分别在开花散粉期前后进行干旱处理和高温处理,以当地玉米种植正常管理为对照,测定不同处理的株高、穗位高、雄穗分枝数、散粉吐丝间隔期、穗长、穗行数、行粒数、百粒重、单株产量和叶绿素含量,计算PSⅡ潜在活性(Fv/Fo)和PSⅡ最大光化学量子产量(F_(v)/F_(m)),对不同种质的耐旱性和耐热性进行综合评价。【结果】不同玉米种质间12个性状的联合方差分析结果显示,种质间及环境(胁迫)间均存在极显著差异(P<0.01,下同)。不同玉米种质间开花散粉期对干旱胁迫响应的变化趋势一致,单株产量和散粉吐丝间隔期受干旱影响较大,穗行数受干旱影响较小。不同玉米种质间开花散粉期对高温胁迫响应的变化趋势一致,单株产量受高温影响较大,穗位高受高温影响较小。叶绿素含量、Fv/Fo和F_(v)/F_(m)的耐旱系数在热带种质中表现最好,雄穗分枝数耐旱系数在温热种质中表现最好,株高和穗位高的耐旱系数在温带种质中表现最好。穗长、单株产量、叶绿素含量、Fv/Fo、F_(v)/F_(m)等性状耐热系数在热带种质中表现最好。3类种质中耐旱以上自交系数量表现为热带种质(11个)>温热种质(9个)>温带种质(7个),中耐热以上自交系数量表现为热带种质(10个)>温热种质(3个)>温带种质(2个)。不同种质耐旱性和耐热性综合评价结果与其聚类分析结果高度一致,且不同种质耐旱性和耐热性加权隶属值均具有极显著相关性。聚类分析结果表明强耐旱自交系单株产量、叶绿素含量、F_(v)/F_(m)、株高、穗长等性状表现较好,强耐热自交系单株产量、穗长、行粒数、叶绿素含量、F_(v)/F_(m)等性状表现较好。加权隶属值结果表明株高、单株产量、叶绿素含量和F_(v)/F_(m)与耐旱性相关性较高,行粒数、单株产量、叶绿素含量、F_(v)/F_(m)与耐热性相关性较高。【结论】不同种质对干旱胁迫和高温胁迫响应趋势相同但响应程度存在差异,热带种质更易筛选出耐旱性或耐热性表现较好的自交系。单株产量、叶绿素含量和F_(v)/F_(m)对耐旱性和耐热性鉴定均具有较好效果。

盐逆境对不同耐盐性小麦花后生理特性及产量的影响

为筛选耐盐高产小麦品种,确定较为快捷有效的小麦耐盐鉴定指标,本研究采用田间试验,以前期筛选出的5个耐盐差异性显著的春性小麦品种(较耐盐品种:‘宁麦21’‘扬麦20’‘扬辐麦4’;盐敏感品种:‘扬麦23’‘安农1124’)为材料,在江苏大丰地区设置非盐逆境(S0,播前土壤含盐量0.770±0.062 g·kg^(−1))和盐逆境(S1,播前土壤含盐量3.294±0.198 g·kg^(−1))两个处理,研究了盐胁迫对不同耐盐性小麦花后生理特性及产量的影响。结果表明,在盐逆境下,春小麦叶面积指数、干物质积累量及茎蘖数显著下降;穗数、每穗粒数与千粒重也显著减少,其中穗数降幅达60.7%,为减产的主导因素;其次是粒重的减少,最终导致产量显著降低,仅为非逆境条件下的26.2%。研究还发现,在盐碱胁迫下,花后SPAD值与叶绿素荧光Fv/Fm降低,丙二醛与脯氨酸含量显著增加,变化幅度在品种间存在差异,耐盐品种的SPAD值及叶绿素荧光Fv/Fm的降幅与丙二醛的增幅较小,但脯氨酸含量的增幅较大,因而减产幅度较小。进一步分析表明,叶绿素荧光Fv/Fm与穗数、千粒重及产量间呈显著正相关关系,SPAD值与干物质积累量呈显著正相关,干物质积累量与穗数、千粒重及产量间呈显著正相关关系。这些结果表明盐胁迫通过降低叶绿素含量与叶绿素荧光Fv/Fm抑制小麦的光合作用,减少光合产物的生成,造成最终的减产。因此在小麦耐盐性田间鉴定中,开花期SPAD值与叶绿素荧光Fv/Fm可作为耐盐性鉴定快捷可靠的指标。此外,‘扬麦20’在盐碱地中减产幅度最小,综合表现较好,适合在大丰地区盐碱地种植。

开花下针期干旱胁迫对花生氮代谢的影响

为揭示干旱胁迫下花生氮代谢的生理响应机制,以4个耐旱性差异的花生品种为材料,即耐旱品种NH9(农花9)和HY22(花育22)、旱敏感品种NH16(农花16)和NH21(农花21),通过测定植株氮素积累量、含氮化合物含量和氮代谢关键酶活性等指标的响应规律,研究了开花下针期干旱胁迫对花生氮代谢的影响。结果表明:干旱胁迫下4个花生品种各器官的氮素积累量均降低,但NH9和HY22的降低幅度较NH16和NH21小;NH9和HY22的硝态氮、脯氨酸和可溶性蛋白含量呈增加趋势,增幅为4.60%~53.77%,而NH16和NH21的含氮化合物含量随干旱胁迫时间延长显著降低;NH9和HY22的硝酸还原酶、谷氨酰胺还原酶、谷氨酸合成酶、谷丙转氨酶和谷草转氨酶的活性明显增强,而NH16和NH21的氮代谢关键酶活性显著下降,降幅为2.99%~46.85%。干旱复水后,NH9和HY22的氮素含量、含氮化合物含量及氮代谢相关酶活性恢复能力较强,而NH16和NH21与正常供水相比仍差异显著。综上,干旱胁迫下耐旱花生品种能通过增强氮代谢关键酶活性,促进氮素同化,提高脯氨酸和可溶性蛋白等含氮化合物的积累,有效减轻干旱损伤。

High-temperature oxidation behavior of DZ125 Ni-based superalloy under tensile stress

The key in antioxidant capacity of aero-engine hot components is the protective oxide scales.Many factors can affect the performance of oxide scales.The effect of tensile stress on high-temperature oxidation behavior of directionally solidified DZ125 Ni-based superalloy was investigated by thermogravimetric analysis(TGA).Tensile samples were subjected to stress of 100 MPa,and the oxidation behavior was studied at 980℃in air.The surface and cross-sectional morphologies of the oxidized coating were analyzed by scanning electron microscope(SEM).The influence of tensile stress on the oxidation behavior of directionally solidified DZ125 Ni-based superalloy at high temperature was discussed.Results show that an applied tensile stress generally leads to larger Cr/Ni mass ratio in the oxide scales,greater overall chromium depletion values directly under the oxide scales,lower specific weight gain values and thinner oxide scales.These factors are attributed to the formation of fast diffusion paths for Cr atoms to diffuse to the surface under tensile stress,thus causing a reduction in the duration of the less protective transient oxidation period and promoting a faster formation of the protective Cr_(2)O_(3)layer.

干旱胁迫对不同小麦品种花后旗叶生理特性的影响

在防雨旱棚池栽条件下研究了干旱胁迫对不同小麦品种旗叶某些生理特性的影响。结果表明,干旱胁迫条件下,小麦开花后旗叶的相对含水量、可溶性蛋白质含量和保护酶系统SOD、CAT和POD活性均降低,膜脂过氧化产物MDA含量升高,产量也随之下降。与水浇地品种相比,旱地品种青麦6号和鲁麦21旗叶的相对含水量、可溶性蛋白质含量相对较高且下降缓慢,SOD、CAT和POD活性降低的幅度比较缓和,随着干旱胁迫MDA含量继续积累的速度缓慢,产量及产量构成因素降低的幅度小。青麦6号与鲁麦21相比,旗叶的相对含水量、可溶性蛋白质含量和SOD、CAT、POD活性较高,MDA含量较低并且最终产量较高,说明青麦6号在干旱胁迫条件下能保持一个相对较优的生理状态,其代谢功能和抗旱能力较强。

土壤水分胁迫对不同品种冬小麦生理特性的影响

为研究水分胁迫条件下不同类型冬小麦品种生理特性的变化特点,在干旱胁迫和正常水分条件下对不同抗旱类型冬小麦品种(青麦6号、鲁麦21、良星99、烟农21、烟农24、济麦22)的旗叶生理特性进行了研究。结果表明:不同小麦品种旗叶在开花后干旱胁迫下的可溶性蛋白含量以及保护酶系统SOD、CAT和POD活性均降低,而可溶性糖含量和丙二醛含量均升高,而且这种降低和升高的幅度随生育进程而增大。旱地品种青麦6号和烟农21在干旱胁迫下旗叶的可溶性糖含量高于其他品种且上升幅度快,青麦6号和鲁麦21可溶性蛋白含量以及SOD、CAT和POD活性均高于其他品种且下降幅度缓慢,而丙二醛含量均低于其他品种且随着生育进程积累速度平缓,产量及产量构成因素降低的幅度小。因此,旱地小麦品种在干旱胁迫条件下能保持一个相对较优的生理状态,其代谢功能和抗旱能力较强,最终获得高产。

闭颖授粉水稻CL01的抗逆性及其相适应的生理特点

本文对开颖授粉水稻材料 T16 8和闭颖授粉水稻材料 CL 0 1进行了比较研究。发现在连续人工降雨和低温处理条件下 ,CL 0 1的结实率受影响较小 ;相反 ,T16 8的结实率随着处理天数的增加而迅速降低 ,说明 CL 0 1的抗逆性较强。通过对从开花前 5天到开花当天的花药和花丝的长度、浆片的大小以及在开花过程中浆片的鲜重和干重的变化的研究 ,揭示了一些与 CL 0 1闭颖授粉相适应的生理特点 ,即从开花前 5天到开花当天 CL 0 1的花药和花丝的长度以及浆片的大小增加均不明显 ,缺少了开颖所需要的机械力 ,因而闭颖。相反 ,T16 8的花药和花丝长度以及浆片的大小增加则相当明显 ;同时 ,在开花的过程中 ,T16 8的浆片鲜重和干重变化明显 ,在颖壳张开角度最大时达到最大值 ,这些因素可能和开颖有很大关系。虽然 CL 0 1的浆片鲜重有一定的变化 ,但是干重基本上没有变化 ,这说明 CL 0 1浆片鲜重的增加基本上是由于浆片吸水造成的 ,其中并不伴随物质的交换。

我国主要玉米自交系开花期耐旱性差异及改良

通过对 37份我国主要玉米自交系两年的开花期耐旱性鉴定 ,筛选出耐旱系 12份 (K2 2、SH15、X178、P138、中自 0 1、中自 4 5 1、金黄 96 B、齐 319、旱 2 3、东 91、临京 11、CA15 6 )。在干旱胁迫下 ,果穗吐丝延迟 ,雌雄开花间隔增大 ,结穗率下降 ,籽粒产量严重降低 ;雌雄开花间隔天数和结穗率与籽粒产量均呈极显著相关 ,是可供耐旱性选择利用的指示性状。用 SSR标记研究了 38份自交系的遗传多样性。 4 7对 SSR引物共检测出 15 6个等位基因变异 ,将供试自交系划为 6个类群。 9份耐旱系分散于 4个类群 (B、C、E、F) ,其中 E群被鉴定为耐旱种质群。