Bocaparvovirus(BOV) is a genetically diverse group of DNA viruses and a possible cause of respiratory, enteric, and neurological diseases in humans and animals. Here, two highly divergent BOVs(tentatively named as Him...Bocaparvovirus(BOV) is a genetically diverse group of DNA viruses and a possible cause of respiratory, enteric, and neurological diseases in humans and animals. Here, two highly divergent BOVs(tentatively named as Himalayan marmot BOV,HMBOV1 and HMBOV2) were identified in the livers and feces of wild Himalayan marmots in China, by viral metagenomic analysis. Five of 300 liver samples from Himalayan marmots were positive for HMBOV1 and five of 99 fecal samples from these animals for HMBOV2. Their nearly complete genome sequences are 4,672 and 4,887 nucleotides long, respectively, with a standard genomic organization and containing protein-coding motifs typical for BOVs. Based on their NS1, NP1, and VP1,HMBOV1 and HMBOV2 are most closely related to porcine BOV SX/1-2(approximately 77.0%/50.0%, 50.0%/53.0%, and79.0%/54.0% amino acid identity, respectively). Phylogenetic analysis of these three proteins showed that HMBOV1 and HMBOV2 formed two distinctly independent branches in BOVs. According to these results, HMBOV1 and HMBOV2 are two different novel species in the Bocaparvovirus genus. Their identification expands our knowledge of the genetic diversity and evolution of BOVs. Further studies are needed to investigate their potential pathogenicity and their impact on Himalayan marmots and humans.展开更多
Objective:This study aims to explore the association between the density of Himalayan marmot (Marmota himalayana) and climatic factors such as temperature, precipitation, humidity, vapour pressure, sunshine percentage...Objective:This study aims to explore the association between the density of Himalayan marmot (Marmota himalayana) and climatic factors such as temperature, precipitation, humidity, vapour pressure, sunshine percentage, wind velocity, which are closely associated with global climate change, and to provide a reference for plague prevention and control. Methods: We conducted a regression analysis to find the possible climate factors associated with the density of Himalaya marmot, and analyzed the response characters of Himalayan marmot to climate change.Results: Dailyprecipitation days(>=0.1 mm) and sunshine percentage were significantly associated with thedensityofHimalayan marmot(p<0.01). Conclusion: Climate change was associated with the risk of plague. This phenomenon is valuable for Himalayan marmot and plague prevention. More studies are needed to understand the impact of climate change on Himalayan marmot and plague.展开更多
Plague,caused by the gram-negative bacterium Yersinia pestis,is a serious and rapidly progressing illness in humans that can be fatal if not treated effectively.The Qinghai-Tibet Plateau is the largest area of natural...Plague,caused by the gram-negative bacterium Yersinia pestis,is a serious and rapidly progressing illness in humans that can be fatal if not treated effectively.The Qinghai-Tibet Plateau is the largest area of natural Himalayan marmot(Marmota himalayana) plague foci in China and covers more than 630000 km2.Akesai County in Gansu Province is a part of this natural focus of plague and was chosen as a study area.Our study used an ecological niche modeling(ENM) approach to predict the potential distribution of the Himalayan marmot.Environment and Disaster Monitor Satellite(HJ-1) data was used to investigate environment factors that affect plague host animal activity.Host animal point data from active surveillance was combined with environmental variables from the HJ-1 satellite and other databases,and the models of the potential distribution of Himalayan marmot were produced with the Genetic Algorithm for Rule-Set Production(GARP).The probability of marmot presence was divided into 0-5%,5%-20%,20%-40%,40%-80%,and 80%-100% subgroups.Areas with 80%-100% probability exhibited the greatest potential for the presence of Himalayan marmot.According to the predicted potential distribution of Himalayan marmot in the study area,active surveillance of plague hosts and plague control and prevention could be more efficient.展开更多
目的观察青海省青藏高原喜马拉雅旱獭分离的布鲁氏菌的多位点可变数目串联重复序列分析(multiple locus variable-number tandem repeat analysis,MLVA)分型,探讨其与既往青海省布鲁氏菌分离菌株间的亲缘关系。方法2019年3月至2020年10...目的观察青海省青藏高原喜马拉雅旱獭分离的布鲁氏菌的多位点可变数目串联重复序列分析(multiple locus variable-number tandem repeat analysis,MLVA)分型,探讨其与既往青海省布鲁氏菌分离菌株间的亲缘关系。方法2019年3月至2020年10月,采集青海省青藏高原喜马拉雅旱獭全血样本,对虎红平板凝集试验(rose bengal test,RBT)布鲁氏菌抗体阳性样本进行病原体分离培养,采用传统生物学方法和分子生物学方法(BCSP31-PCR和AMOS-PCR方法)进行菌株鉴定。同时,应用MLVA方法对分离菌株进行基因分型,并结合既往青海省不同宿主分离的70株布鲁氏菌的基因型,应用聚类分析方法探讨菌株间的亲缘关系。结果共采集青藏高原喜马拉雅旱獭全血样本1466份,从其中64份RBT阳性样本中分离培养出2株布鲁氏菌,分别命名为QH2013054、QH2013062,经传统生物学和分子生物学方法鉴定为羊种布鲁氏菌生物Ⅲ型。MLVA分型结果显示,QH2013054与QH2013062菌株在Bru16位点有差异,为不同的MLVA基因型。聚类分析结果显示,QH2013054菌株与7株菌株具有相同的MLVA基因型,其中6株来自共和县同一个家庭的3名农民和3只羊,1株来自门源回族自治县农民;QH2013062菌株与4株菌株具有相同的MLVA基因型,其中3株来自门源回族自治县的3名农民,1株来自互助土族自治县农民。结论从青海省青藏高原喜马拉雅旱獭中分离的布鲁氏菌与在本省人、羊中分离的部分布鲁氏菌具有相同的MLVA基因型,推测宿主人、羊、青藏高原喜马拉雅旱獭可能具有共同的传染源。展开更多
基金funded by the Special National Project on Research and Development of Key Biosafety Technologies(2016YFC1201900)the National Natural Science Foundation of China(81290345)
文摘Bocaparvovirus(BOV) is a genetically diverse group of DNA viruses and a possible cause of respiratory, enteric, and neurological diseases in humans and animals. Here, two highly divergent BOVs(tentatively named as Himalayan marmot BOV,HMBOV1 and HMBOV2) were identified in the livers and feces of wild Himalayan marmots in China, by viral metagenomic analysis. Five of 300 liver samples from Himalayan marmots were positive for HMBOV1 and five of 99 fecal samples from these animals for HMBOV2. Their nearly complete genome sequences are 4,672 and 4,887 nucleotides long, respectively, with a standard genomic organization and containing protein-coding motifs typical for BOVs. Based on their NS1, NP1, and VP1,HMBOV1 and HMBOV2 are most closely related to porcine BOV SX/1-2(approximately 77.0%/50.0%, 50.0%/53.0%, and79.0%/54.0% amino acid identity, respectively). Phylogenetic analysis of these three proteins showed that HMBOV1 and HMBOV2 formed two distinctly independent branches in BOVs. According to these results, HMBOV1 and HMBOV2 are two different novel species in the Bocaparvovirus genus. Their identification expands our knowledge of the genetic diversity and evolution of BOVs. Further studies are needed to investigate their potential pathogenicity and their impact on Himalayan marmots and humans.
基金The Basic Application Project of Qinghai Provincegrant number:2013-Z-747
文摘Objective:This study aims to explore the association between the density of Himalayan marmot (Marmota himalayana) and climatic factors such as temperature, precipitation, humidity, vapour pressure, sunshine percentage, wind velocity, which are closely associated with global climate change, and to provide a reference for plague prevention and control. Methods: We conducted a regression analysis to find the possible climate factors associated with the density of Himalaya marmot, and analyzed the response characters of Himalayan marmot to climate change.Results: Dailyprecipitation days(>=0.1 mm) and sunshine percentage were significantly associated with thedensityofHimalayan marmot(p<0.01). Conclusion: Climate change was associated with the risk of plague. This phenomenon is valuable for Himalayan marmot and plague prevention. More studies are needed to understand the impact of climate change on Himalayan marmot and plague.
基金supported by the Special Grant for Prevention and Treatment of Infectious Diseases (Grant No.2008ZX10004-012)National Natural Science Foundation of China (Grant No.40871173)
文摘Plague,caused by the gram-negative bacterium Yersinia pestis,is a serious and rapidly progressing illness in humans that can be fatal if not treated effectively.The Qinghai-Tibet Plateau is the largest area of natural Himalayan marmot(Marmota himalayana) plague foci in China and covers more than 630000 km2.Akesai County in Gansu Province is a part of this natural focus of plague and was chosen as a study area.Our study used an ecological niche modeling(ENM) approach to predict the potential distribution of the Himalayan marmot.Environment and Disaster Monitor Satellite(HJ-1) data was used to investigate environment factors that affect plague host animal activity.Host animal point data from active surveillance was combined with environmental variables from the HJ-1 satellite and other databases,and the models of the potential distribution of Himalayan marmot were produced with the Genetic Algorithm for Rule-Set Production(GARP).The probability of marmot presence was divided into 0-5%,5%-20%,20%-40%,40%-80%,and 80%-100% subgroups.Areas with 80%-100% probability exhibited the greatest potential for the presence of Himalayan marmot.According to the predicted potential distribution of Himalayan marmot in the study area,active surveillance of plague hosts and plague control and prevention could be more efficient.
文摘目的观察青海省青藏高原喜马拉雅旱獭分离的布鲁氏菌的多位点可变数目串联重复序列分析(multiple locus variable-number tandem repeat analysis,MLVA)分型,探讨其与既往青海省布鲁氏菌分离菌株间的亲缘关系。方法2019年3月至2020年10月,采集青海省青藏高原喜马拉雅旱獭全血样本,对虎红平板凝集试验(rose bengal test,RBT)布鲁氏菌抗体阳性样本进行病原体分离培养,采用传统生物学方法和分子生物学方法(BCSP31-PCR和AMOS-PCR方法)进行菌株鉴定。同时,应用MLVA方法对分离菌株进行基因分型,并结合既往青海省不同宿主分离的70株布鲁氏菌的基因型,应用聚类分析方法探讨菌株间的亲缘关系。结果共采集青藏高原喜马拉雅旱獭全血样本1466份,从其中64份RBT阳性样本中分离培养出2株布鲁氏菌,分别命名为QH2013054、QH2013062,经传统生物学和分子生物学方法鉴定为羊种布鲁氏菌生物Ⅲ型。MLVA分型结果显示,QH2013054与QH2013062菌株在Bru16位点有差异,为不同的MLVA基因型。聚类分析结果显示,QH2013054菌株与7株菌株具有相同的MLVA基因型,其中6株来自共和县同一个家庭的3名农民和3只羊,1株来自门源回族自治县农民;QH2013062菌株与4株菌株具有相同的MLVA基因型,其中3株来自门源回族自治县的3名农民,1株来自互助土族自治县农民。结论从青海省青藏高原喜马拉雅旱獭中分离的布鲁氏菌与在本省人、羊中分离的部分布鲁氏菌具有相同的MLVA基因型,推测宿主人、羊、青藏高原喜马拉雅旱獭可能具有共同的传染源。
