[Objectives]The research aimed to compare antioxidation abilities of different extraction phases of Hippophae rhamnoides L. leaves extract and find its active marker. [Methods] H. rhamnoides L. leaves were extracted f...[Objectives]The research aimed to compare antioxidation abilities of different extraction phases of Hippophae rhamnoides L. leaves extract and find its active marker. [Methods] H. rhamnoides L. leaves were extracted firstly by 75% of ethanol,and then were extracted by petroleum ether,chloroform,ethyl acetate and n-butanol in turn after decompression concentration. Via DPPH and ABTS^+ radical scavenging experiments and Fe^(3+) reduction experiment,antioxidation activity of each phase was evaluated. And chemical compositions related to its antioxidation activity were determined by using UPLC for qualitative identification. [Results] Antioxidation activity sequence of each phase was ethyl acetate phase≈n-butanol phase > alcohol extract of raw material > water phase > chloroform phase > petroleum ether phase. 33 kinds of chemical compositions were isolated from alcohol extract and extracts by using UPLC. [Conclusions] UPLC and antioxidation activity test were used simultaneously to analyze alcohol extract and extracts of H. rhamnoides L. leaves,which could fast and accurately determine extraction phase with the strongest antioxidation ability. The research is significant to study active ingredients and mechanism of antioxidation from H. rhamnoides L. leaves.展开更多
通过采用热水提取乙醇沉淀获得沙棘叶水溶性粗多糖,再经酸性乙醇分级,将沙棘叶水溶性粗多糖分为三个级分:SJ1、SJ2和SJ3,经PC和GC分析都含有X y l(木糖)、A ra(阿拉伯糖)、G lc(葡萄糖)、M an(甘露糖)、G a l(半乳糖)、G a lA(半乳糖醛...通过采用热水提取乙醇沉淀获得沙棘叶水溶性粗多糖,再经酸性乙醇分级,将沙棘叶水溶性粗多糖分为三个级分:SJ1、SJ2和SJ3,经PC和GC分析都含有X y l(木糖)、A ra(阿拉伯糖)、G lc(葡萄糖)、M an(甘露糖)、G a l(半乳糖)、G a lA(半乳糖醛酸).对粗多糖及SJ2脱蛋白后的多糖SJ2′进行清除自由基活性研究,结果表明,粗多糖对三种自由基的清除力很小,而SJ2′能有效清除.OH、O2-.自由基,对脂质自由基R.效果不明显.展开更多
基金Supported by Application Technology Research and Development Fund Project in Aba Prefecture(33)Science and Technology Innovation Program for Postgraduate of Southwest Minzu University(CX2016SZ031)
文摘[Objectives]The research aimed to compare antioxidation abilities of different extraction phases of Hippophae rhamnoides L. leaves extract and find its active marker. [Methods] H. rhamnoides L. leaves were extracted firstly by 75% of ethanol,and then were extracted by petroleum ether,chloroform,ethyl acetate and n-butanol in turn after decompression concentration. Via DPPH and ABTS^+ radical scavenging experiments and Fe^(3+) reduction experiment,antioxidation activity of each phase was evaluated. And chemical compositions related to its antioxidation activity were determined by using UPLC for qualitative identification. [Results] Antioxidation activity sequence of each phase was ethyl acetate phase≈n-butanol phase > alcohol extract of raw material > water phase > chloroform phase > petroleum ether phase. 33 kinds of chemical compositions were isolated from alcohol extract and extracts by using UPLC. [Conclusions] UPLC and antioxidation activity test were used simultaneously to analyze alcohol extract and extracts of H. rhamnoides L. leaves,which could fast and accurately determine extraction phase with the strongest antioxidation ability. The research is significant to study active ingredients and mechanism of antioxidation from H. rhamnoides L. leaves.
文摘通过采用热水提取乙醇沉淀获得沙棘叶水溶性粗多糖,再经酸性乙醇分级,将沙棘叶水溶性粗多糖分为三个级分:SJ1、SJ2和SJ3,经PC和GC分析都含有X y l(木糖)、A ra(阿拉伯糖)、G lc(葡萄糖)、M an(甘露糖)、G a l(半乳糖)、G a lA(半乳糖醛酸).对粗多糖及SJ2脱蛋白后的多糖SJ2′进行清除自由基活性研究,结果表明,粗多糖对三种自由基的清除力很小,而SJ2′能有效清除.OH、O2-.自由基,对脂质自由基R.效果不明显.
