The investigation of the mechanisms of plant adaptation to stressor action is one of the leading directions of current biological studies. To understand the mechanism of salt tolerance of seabuckthom (Hippophae rhamn...The investigation of the mechanisms of plant adaptation to stressor action is one of the leading directions of current biological studies. To understand the mechanism of salt tolerance of seabuckthom (Hippophae rhamnoides L.) and identify its ability to cope with the salinity effect in the saline and extremely cold region of Qinghai, China, a test was conducted with two-year-old seedlings subjected to 0, 200, 400 and 600 mmol·L^-1 NaClsolutions for 30 d. The results show that with an increase in salinity, the biomass of H. rhamnoides seedlings clearly decreased. Leaf water potential (ψw) and relative water content (RWC) were significantly reduced under salinity, with severe water shortages appearing in leaves. At the same time, the total chlorophyll content declined markedly. When salinity increased and stress time prolonged, the net CO2 assimilation rate (A) significantly declined. Intercellular CO2 concentration (Ci) declined at first and was then followed by an increase over the stress time. We conclude that H. rhamnoides grown in the extremely cold and saline region of Qinghai has a certain resistance to salt, which can be planted at appropriate salinity levels.展开更多
通过采用热水提取乙醇沉淀获得沙棘叶水溶性粗多糖,再经酸性乙醇分级,将沙棘叶水溶性粗多糖分为三个级分:SJ1、SJ2和SJ3,经PC和GC分析都含有X y l(木糖)、A ra(阿拉伯糖)、G lc(葡萄糖)、M an(甘露糖)、G a l(半乳糖)、G a lA(半乳糖醛...通过采用热水提取乙醇沉淀获得沙棘叶水溶性粗多糖,再经酸性乙醇分级,将沙棘叶水溶性粗多糖分为三个级分:SJ1、SJ2和SJ3,经PC和GC分析都含有X y l(木糖)、A ra(阿拉伯糖)、G lc(葡萄糖)、M an(甘露糖)、G a l(半乳糖)、G a lA(半乳糖醛酸).对粗多糖及SJ2脱蛋白后的多糖SJ2′进行清除自由基活性研究,结果表明,粗多糖对三种自由基的清除力很小,而SJ2′能有效清除.OH、O2-.自由基,对脂质自由基R.效果不明显.展开更多
基金supported by the Doctoral Program Foundation of Institutions of Higher Education of China (20070022028)the 11th Five Scientific & Technological Sustaining Research Program of China (2006BAD03A1203)
文摘The investigation of the mechanisms of plant adaptation to stressor action is one of the leading directions of current biological studies. To understand the mechanism of salt tolerance of seabuckthom (Hippophae rhamnoides L.) and identify its ability to cope with the salinity effect in the saline and extremely cold region of Qinghai, China, a test was conducted with two-year-old seedlings subjected to 0, 200, 400 and 600 mmol·L^-1 NaClsolutions for 30 d. The results show that with an increase in salinity, the biomass of H. rhamnoides seedlings clearly decreased. Leaf water potential (ψw) and relative water content (RWC) were significantly reduced under salinity, with severe water shortages appearing in leaves. At the same time, the total chlorophyll content declined markedly. When salinity increased and stress time prolonged, the net CO2 assimilation rate (A) significantly declined. Intercellular CO2 concentration (Ci) declined at first and was then followed by an increase over the stress time. We conclude that H. rhamnoides grown in the extremely cold and saline region of Qinghai has a certain resistance to salt, which can be planted at appropriate salinity levels.
文摘通过采用热水提取乙醇沉淀获得沙棘叶水溶性粗多糖,再经酸性乙醇分级,将沙棘叶水溶性粗多糖分为三个级分:SJ1、SJ2和SJ3,经PC和GC分析都含有X y l(木糖)、A ra(阿拉伯糖)、G lc(葡萄糖)、M an(甘露糖)、G a l(半乳糖)、G a lA(半乳糖醛酸).对粗多糖及SJ2脱蛋白后的多糖SJ2′进行清除自由基活性研究,结果表明,粗多糖对三种自由基的清除力很小,而SJ2′能有效清除.OH、O2-.自由基,对脂质自由基R.效果不明显.