A Hippophae rhamnoides Linn oil nanoemulsion was developed, and its physicochemical properties and in vitro transdermal characteristics were investigated. Then its skin irritation was investigated. The optimum Hippoph...A Hippophae rhamnoides Linn oil nanoemulsion was developed, and its physicochemical properties and in vitro transdermal characteristics were investigated. Then its skin irritation was investigated. The optimum Hippophae rhamnoides Linn oil nanoemulsion prescription was determined using Cremophor EL as an emulsifier, Transcutol P as a co-emulsifier, and isopropyl myristate(IPM) as the oil phase(IPM%: Cremophor EL%: Hippophae rhamnoides Linn oil%= 5:16:20, Km = 4:1). The prepared Hippophae rhamnoides Linn oil nanoemulsion was pale yellow and transparent. Globular droplets were observed under a transmission electron microscope(TEM). The average particle size was 52.2±4.8 nm. The loading capacity was 10.68%, and the oil-in-water(O/W) type nanoemulsion was stable after centrifugation. The CLSM results showed that the fluorescence intensity of the OB Hippophae rhamnoides Linn oil nanoemulsion group was stronger than that of the Hippophae rhamnoides Linn oil cream and Hippophae rhamnoides Linn oil groups in each layer of skin. The study also showed that skin surface treated with the Hippophae rhamnoides Linn oil nanoemulsion had strong fluorescence. The skin depth had weak fluorescence, while the areas near the hair follicle and its appendages had the strongest fluorescence. The skin irritation test showed that the prepared Hippophae rhamnoides Linn oil nanoemulsion was applied to the normal and damaged skin of the rabbit, and there was no apparent redness and swelling. These results suggested that the nanoemulsion coated with Hippophae rhamnoides Linn oil was a potential delivery system for skin disorders.展开更多
基金National Natural Science Foundation of China(Grant No.81460539)the excellent postgraduate project in Xinjiang Medical University(Grant No.CXCY096)
文摘A Hippophae rhamnoides Linn oil nanoemulsion was developed, and its physicochemical properties and in vitro transdermal characteristics were investigated. Then its skin irritation was investigated. The optimum Hippophae rhamnoides Linn oil nanoemulsion prescription was determined using Cremophor EL as an emulsifier, Transcutol P as a co-emulsifier, and isopropyl myristate(IPM) as the oil phase(IPM%: Cremophor EL%: Hippophae rhamnoides Linn oil%= 5:16:20, Km = 4:1). The prepared Hippophae rhamnoides Linn oil nanoemulsion was pale yellow and transparent. Globular droplets were observed under a transmission electron microscope(TEM). The average particle size was 52.2±4.8 nm. The loading capacity was 10.68%, and the oil-in-water(O/W) type nanoemulsion was stable after centrifugation. The CLSM results showed that the fluorescence intensity of the OB Hippophae rhamnoides Linn oil nanoemulsion group was stronger than that of the Hippophae rhamnoides Linn oil cream and Hippophae rhamnoides Linn oil groups in each layer of skin. The study also showed that skin surface treated with the Hippophae rhamnoides Linn oil nanoemulsion had strong fluorescence. The skin depth had weak fluorescence, while the areas near the hair follicle and its appendages had the strongest fluorescence. The skin irritation test showed that the prepared Hippophae rhamnoides Linn oil nanoemulsion was applied to the normal and damaged skin of the rabbit, and there was no apparent redness and swelling. These results suggested that the nanoemulsion coated with Hippophae rhamnoides Linn oil was a potential delivery system for skin disorders.
