Cancer cells with immunogenic properties having altered protein glycosylation, modified blood group substances have been widely studied. Due to the genetic instability occurring during carcinogenesis the glycosyltrans...Cancer cells with immunogenic properties having altered protein glycosylation, modified blood group substances have been widely studied. Due to the genetic instability occurring during carcinogenesis the glycosyltransferases may suffer from posttranslation sequence modification. The author describes 2 autopsy cases, where in the background of the unusual metastatic tumor presentation, incompatible blood group antigenic determinants have been demonstrated using blood group specific lectins and monoclonal antibodies (mAb). In the first case, reported here, a 10-year-old girl developed an acute myeloid leukemia and died in a septic endotoxin shock after successful cytostatic treatment of a juvenile signet ring cell cancer of her colon. At autopsy there were no signs of tumor except bilateral apple-sized mucinous ovarian (Krukenberg) metastases. While she had erythrocyte phenotype of blood group A, the signet ring adenocarcinoma cells expressed blood group B incompatible antigenic determinants with lectin/mAb. In the second case, the autopsy of a 78-year-old female resulted in no macroscopic tumor sign except a moderately enlarged, ham hard spleen. Light microscopy revealed adenocarcinomatous infiltration in the splenic sinusoids. The patient had blood group O, while the metastatic cells in the spleen reacted with Breast Carcinoma Antigen (BioGenex) and incompatible anti-B Banderiaeasimplicifolia agglutinin I and anti-B mAb. It proved to be a case of an occult, completely regressed breast cancer. Based on these observations the expression of tumor specific incompatible blood group antigens might occur from time to time, mostly in adenocarcinomas. Accordingly, blood group-based specific immuno-oncotherapy could be considered in some cancer cases.展开更多
目的了解长牡蛎(Crassostrea gigas)消化道组织中类组织血型抗原(histo-blood group antigens,HBGAs)的类型特点,分析其与诺如病毒的结合特性,以探讨长牡蛎富集诺如病毒的机制。方法利用8种HBGAs单克隆抗体,建立长牡蛎中类HBGAs检测的EL...目的了解长牡蛎(Crassostrea gigas)消化道组织中类组织血型抗原(histo-blood group antigens,HBGAs)的类型特点,分析其与诺如病毒的结合特性,以探讨长牡蛎富集诺如病毒的机制。方法利用8种HBGAs单克隆抗体,建立长牡蛎中类HBGAs检测的ELISA方法,并分析其主要型别。同时,利用5种体外表达的GII.4型诺如病毒P粒子分析其与长牡蛎中类HBGA的结合特性。结果长牡蛎消化道组织中存在类A,H1,Lea和Ley型HBGA;55019株(2006b变异株)和97-1l株(95/96US变异株)GII.4型诺如病毒P粒子可通过类A、H1和Ley型HBGA与长牡蛎消化道组织相结合,91(Camberwell_91株)和42(Hunter_2004)株可通过类Ley型HBGA与长牡蛎消化道组织相结合,156株(sakai株)不与任何类型类HBGA结合。结论长牡蛎消化道组织中存在类A、H1、Lea和Ley型HBGA,GII.4型诺如病毒主要通过类A、H1和Ley型HBGA与长牡蛎消化道组织相结合。展开更多
人类诺如病毒(Noroviruses,NoV)归属于杯状病毒家族,是全球暴发性非细菌性胃肠炎的重要致病原。以往的研究提示宿主遗传因素可能与NoV易感性有关,近几年发现组织血型抗原(histo-blood group antigens,HBGAs)可以作为NoV的结合受体被识别...人类诺如病毒(Noroviruses,NoV)归属于杯状病毒家族,是全球暴发性非细菌性胃肠炎的重要致病原。以往的研究提示宿主遗传因素可能与NoV易感性有关,近几年发现组织血型抗原(histo-blood group antigens,HBGAs)可以作为NoV的结合受体被识别,该领域的研究不仅部分揭示不同个体对NoV易感性的差异,而且对于探索NoV感染的发病机制、流行病学规律和防治措施有重要意义。[临床儿科杂志,2007,25(12):1036-1039]展开更多
文摘Cancer cells with immunogenic properties having altered protein glycosylation, modified blood group substances have been widely studied. Due to the genetic instability occurring during carcinogenesis the glycosyltransferases may suffer from posttranslation sequence modification. The author describes 2 autopsy cases, where in the background of the unusual metastatic tumor presentation, incompatible blood group antigenic determinants have been demonstrated using blood group specific lectins and monoclonal antibodies (mAb). In the first case, reported here, a 10-year-old girl developed an acute myeloid leukemia and died in a septic endotoxin shock after successful cytostatic treatment of a juvenile signet ring cell cancer of her colon. At autopsy there were no signs of tumor except bilateral apple-sized mucinous ovarian (Krukenberg) metastases. While she had erythrocyte phenotype of blood group A, the signet ring adenocarcinoma cells expressed blood group B incompatible antigenic determinants with lectin/mAb. In the second case, the autopsy of a 78-year-old female resulted in no macroscopic tumor sign except a moderately enlarged, ham hard spleen. Light microscopy revealed adenocarcinomatous infiltration in the splenic sinusoids. The patient had blood group O, while the metastatic cells in the spleen reacted with Breast Carcinoma Antigen (BioGenex) and incompatible anti-B Banderiaeasimplicifolia agglutinin I and anti-B mAb. It proved to be a case of an occult, completely regressed breast cancer. Based on these observations the expression of tumor specific incompatible blood group antigens might occur from time to time, mostly in adenocarcinomas. Accordingly, blood group-based specific immuno-oncotherapy could be considered in some cancer cases.
文摘目的了解长牡蛎(Crassostrea gigas)消化道组织中类组织血型抗原(histo-blood group antigens,HBGAs)的类型特点,分析其与诺如病毒的结合特性,以探讨长牡蛎富集诺如病毒的机制。方法利用8种HBGAs单克隆抗体,建立长牡蛎中类HBGAs检测的ELISA方法,并分析其主要型别。同时,利用5种体外表达的GII.4型诺如病毒P粒子分析其与长牡蛎中类HBGA的结合特性。结果长牡蛎消化道组织中存在类A,H1,Lea和Ley型HBGA;55019株(2006b变异株)和97-1l株(95/96US变异株)GII.4型诺如病毒P粒子可通过类A、H1和Ley型HBGA与长牡蛎消化道组织相结合,91(Camberwell_91株)和42(Hunter_2004)株可通过类Ley型HBGA与长牡蛎消化道组织相结合,156株(sakai株)不与任何类型类HBGA结合。结论长牡蛎消化道组织中存在类A、H1、Lea和Ley型HBGA,GII.4型诺如病毒主要通过类A、H1和Ley型HBGA与长牡蛎消化道组织相结合。
文摘人类诺如病毒(Noroviruses,NoV)归属于杯状病毒家族,是全球暴发性非细菌性胃肠炎的重要致病原。以往的研究提示宿主遗传因素可能与NoV易感性有关,近几年发现组织血型抗原(histo-blood group antigens,HBGAs)可以作为NoV的结合受体被识别,该领域的研究不仅部分揭示不同个体对NoV易感性的差异,而且对于探索NoV感染的发病机制、流行病学规律和防治措施有重要意义。[临床儿科杂志,2007,25(12):1036-1039]
