AIM: To investigate effects of severe burn injury(BI) in rat liver through the histopathological and inflammatory markers analysis. METHODS: Forty-two male Wistar rats were distributed into two groups, control(C) and ...AIM: To investigate effects of severe burn injury(BI) in rat liver through the histopathological and inflammatory markers analysis. METHODS: Forty-two male Wistar rats were distributed into two groups, control(C) and subjected to scald BI(SBI). The animals were euthanized one, four and 14 d post sham or 45% of the total body surface BI. Liver fragments were submitted to histopathological, morphoquantitative(hepatocyte area and cell density), ciclooxigenase-2(COX-2) immunoexpression, and gene expression [real-time polymerase chain reaction for tumor necrosis factor(TNF)-α, inducible nitric oxide synthase(i NOS) and caspase-3] methods. RESULTS: Histopathological findings showed inflammatory process in all periods investigated and hepatocyte degeneration added to increased amount of connective tissue 14 d post injury. Hepatocyte area, the density of binucleated hepatocytes and density of sinusoidal cells of SBI groups were increased when compared with control. COX-2 immunoexpression was stronger in SBI groups. No differences were found in TNF-α, i NOS and caspase-3 gene expression. CONCLUSION: BI induces histopathological changes, upregulation of COX-2 immunoexpression, and cell proliferation in liver of rats.展开更多
OBJECTIVE To investigate whether soy milk may cause reproductive disorders and decrease testosterone.METHODS Thirty two six weeks old male rats were divided into 4 groups including control group(non treatment)and thre...OBJECTIVE To investigate whether soy milk may cause reproductive disorders and decrease testosterone.METHODS Thirty two six weeks old male rats were divided into 4 groups including control group(non treatment)and three other groups were treated with soy milk powder(7.1,14.2and 21.3g·kg-1)everyday for 90 d.Histopathological examination of testis,epididymis and seminal vesicles were done using HE staining.Blood testosterone levels were assayed by ELISA.RESULTS There were positive correlations between the doses of soy milk with spermatogenesis in the testes,prostate epithelial cell hyperplasia.There were also positively correlation between dose of soy milk with vacuoles forming on epididymal epithelial cells and apoptosis in epithelial cells of seminal vesicles.The blood testosterone levels were not significantly difference between groups.CONCLUSION Subchronically soy milk feeding in rats induce histopathology changes of reproductive organs that closely related to the process of endocrine disruptors.展开更多
基金Sao Paulo Research Foundation,FAPESP,No.11/22034-9
文摘AIM: To investigate effects of severe burn injury(BI) in rat liver through the histopathological and inflammatory markers analysis. METHODS: Forty-two male Wistar rats were distributed into two groups, control(C) and subjected to scald BI(SBI). The animals were euthanized one, four and 14 d post sham or 45% of the total body surface BI. Liver fragments were submitted to histopathological, morphoquantitative(hepatocyte area and cell density), ciclooxigenase-2(COX-2) immunoexpression, and gene expression [real-time polymerase chain reaction for tumor necrosis factor(TNF)-α, inducible nitric oxide synthase(i NOS) and caspase-3] methods. RESULTS: Histopathological findings showed inflammatory process in all periods investigated and hepatocyte degeneration added to increased amount of connective tissue 14 d post injury. Hepatocyte area, the density of binucleated hepatocytes and density of sinusoidal cells of SBI groups were increased when compared with control. COX-2 immunoexpression was stronger in SBI groups. No differences were found in TNF-α, i NOS and caspase-3 gene expression. CONCLUSION: BI induces histopathological changes, upregulation of COX-2 immunoexpression, and cell proliferation in liver of rats.
文摘OBJECTIVE To investigate whether soy milk may cause reproductive disorders and decrease testosterone.METHODS Thirty two six weeks old male rats were divided into 4 groups including control group(non treatment)and three other groups were treated with soy milk powder(7.1,14.2and 21.3g·kg-1)everyday for 90 d.Histopathological examination of testis,epididymis and seminal vesicles were done using HE staining.Blood testosterone levels were assayed by ELISA.RESULTS There were positive correlations between the doses of soy milk with spermatogenesis in the testes,prostate epithelial cell hyperplasia.There were also positively correlation between dose of soy milk with vacuoles forming on epididymal epithelial cells and apoptosis in epithelial cells of seminal vesicles.The blood testosterone levels were not significantly difference between groups.CONCLUSION Subchronically soy milk feeding in rats induce histopathology changes of reproductive organs that closely related to the process of endocrine disruptors.
