Objective:To evaluate the berries of Phytolacca dodecundra(P.dodecandra) for its effect on Histoplasma cupsulatum var.farciminosum(HCF) and for the treatment of cases of epizootic lymphangitis(ELi.Methods:Samples were...Objective:To evaluate the berries of Phytolacca dodecundra(P.dodecandra) for its effect on Histoplasma cupsulatum var.farciminosum(HCF) and for the treatment of cases of epizootic lymphangitis(ELi.Methods:Samples were collected from un-ruptured nodules of cases of EL at Debre Zeit and Akaki(central Ethiopia).Mycologieal culture and isolation of HCF were performed at the Akliln Lemma Institute of Pathobiology.Phytochemical screening was done for n-butanol extract of P.dodecandra to delect alkaloids,saponins,phenolic compounds and flavonoids.The minimum inhibitory concentrations(MICs) and minimum fungicidal concentrations(MFCs) ol aqueous and n-butanol extracts of P.dodecandra against FICF were determined by agar dilution assay.For the in vivo trial.5%simple ointment was prepared from n-butanol extract and applied topically to 24(twelve early and twelve moderate) cases of F.L.Results:Phytochemical screening showed that n-butanol extract ol P.dodecandra was positive lor alkaloids.saponins and phenolic compounds but negative for flavonoids.The MFCs of n-butanol and aqueous extracts of P.dodecandra were(0.039%-0.078%) and(0.625%-1.250%),respectively.The MFCs of n-butanol and aqueous extracts of P.dodecandra were(0.078%t-0.156%)and(1.250%-2.500%),respectively.The MIC and MFC of ketoconazole(positive control) was(1.200×10^(-5)%-2.500×10^(-5)%) and(5.000× 10^(-5)%-1.000×10^(-4)%),respectively while growth was observed on free medium(negative control).From the total of 24 treated cases of EL,14(58.3%) responded lo treatment;however,10(41.7%) did not respond to treatment.There was no significant difference in the degree of response to treatment between early and moderate cases(χ~2=0.086:P=0.408.Conclusions:It can be concluded that n-butanol extract of P.dodecandra demonstrates antifungal effects while the aqueous extract shows no antifungal activity.展开更多
This paper communicates the presence of aberrant microscopic forms of Histoplasma capsulatum in a sample obtained by scrapping of the skin lesion and stained with a rapid modification of the Grocott technique.The AIDS...This paper communicates the presence of aberrant microscopic forms of Histoplasma capsulatum in a sample obtained by scrapping of the skin lesion and stained with a rapid modification of the Grocott technique.The AIDS patient was treated with cream contained corticoids and antifungal and antibacterial antibiotics by an erroneous diagnosis.Once the etiologic diagnosis was achieved,oral itraconazol was administrated at the daily dose of 400 mg during at least six months.These non habitual forms described in the literature as " aberrant variants", can be interpreted as an "adaptive phenomenon" of this termodimorphic fungal specie,as response of the ecologic alterations produced by antibiotic on the local antagonic bacterial microbiota,the deleterous activity of antifungal on the fungal cells and the local inmunodepression produced by the corticoids.展开更多
Histoplasmosis is an opportunistic granulomatous fungal infection. Peritoneal histoplasmosis (PH) is a rare form. The first case of PH was described in 1970 but this is the first case reported in Mauritania. We report...Histoplasmosis is an opportunistic granulomatous fungal infection. Peritoneal histoplasmosis (PH) is a rare form. The first case of PH was described in 1970 but this is the first case reported in Mauritania. We report the case of a 60-year-old male patient with a history of pulmonary tuberculosis, treated and declared cured, and partial epileptic seizures treated with <em>Carbamazepine</em>. Contrast computed tomography of the abdomen showed a large mass with a large intraperitoneal fluid component with a finely calcified wall in places, for which laparoscopy and biopsy were performed, identifying <em>Histoplasma capsulatum</em> infection. The subject received treatment with <em>amphotericin</em> <em>B deoxycholate</em> with good evolution, and outpatient management with<em> itraconazole</em>. PH is a rare entity that requires high clinical suspicion, especially in immunocompetent patients. The patient was informed that non-identifying information from the case would be submitted for publication, and he provided consent.展开更多
基金supported by the Graduate Program the Addis Ababa University and Aklilu Lemma Institute of Pathobiology Addis Ababa University(grant No.CAOR/PY-059/2011)
文摘Objective:To evaluate the berries of Phytolacca dodecundra(P.dodecandra) for its effect on Histoplasma cupsulatum var.farciminosum(HCF) and for the treatment of cases of epizootic lymphangitis(ELi.Methods:Samples were collected from un-ruptured nodules of cases of EL at Debre Zeit and Akaki(central Ethiopia).Mycologieal culture and isolation of HCF were performed at the Akliln Lemma Institute of Pathobiology.Phytochemical screening was done for n-butanol extract of P.dodecandra to delect alkaloids,saponins,phenolic compounds and flavonoids.The minimum inhibitory concentrations(MICs) and minimum fungicidal concentrations(MFCs) ol aqueous and n-butanol extracts of P.dodecandra against FICF were determined by agar dilution assay.For the in vivo trial.5%simple ointment was prepared from n-butanol extract and applied topically to 24(twelve early and twelve moderate) cases of F.L.Results:Phytochemical screening showed that n-butanol extract ol P.dodecandra was positive lor alkaloids.saponins and phenolic compounds but negative for flavonoids.The MFCs of n-butanol and aqueous extracts of P.dodecandra were(0.039%-0.078%) and(0.625%-1.250%),respectively.The MFCs of n-butanol and aqueous extracts of P.dodecandra were(0.078%t-0.156%)and(1.250%-2.500%),respectively.The MIC and MFC of ketoconazole(positive control) was(1.200×10^(-5)%-2.500×10^(-5)%) and(5.000× 10^(-5)%-1.000×10^(-4)%),respectively while growth was observed on free medium(negative control).From the total of 24 treated cases of EL,14(58.3%) responded lo treatment;however,10(41.7%) did not respond to treatment.There was no significant difference in the degree of response to treatment between early and moderate cases(χ~2=0.086:P=0.408.Conclusions:It can be concluded that n-butanol extract of P.dodecandra demonstrates antifungal effects while the aqueous extract shows no antifungal activity.
文摘This paper communicates the presence of aberrant microscopic forms of Histoplasma capsulatum in a sample obtained by scrapping of the skin lesion and stained with a rapid modification of the Grocott technique.The AIDS patient was treated with cream contained corticoids and antifungal and antibacterial antibiotics by an erroneous diagnosis.Once the etiologic diagnosis was achieved,oral itraconazol was administrated at the daily dose of 400 mg during at least six months.These non habitual forms described in the literature as " aberrant variants", can be interpreted as an "adaptive phenomenon" of this termodimorphic fungal specie,as response of the ecologic alterations produced by antibiotic on the local antagonic bacterial microbiota,the deleterous activity of antifungal on the fungal cells and the local inmunodepression produced by the corticoids.
文摘Histoplasmosis is an opportunistic granulomatous fungal infection. Peritoneal histoplasmosis (PH) is a rare form. The first case of PH was described in 1970 but this is the first case reported in Mauritania. We report the case of a 60-year-old male patient with a history of pulmonary tuberculosis, treated and declared cured, and partial epileptic seizures treated with <em>Carbamazepine</em>. Contrast computed tomography of the abdomen showed a large mass with a large intraperitoneal fluid component with a finely calcified wall in places, for which laparoscopy and biopsy were performed, identifying <em>Histoplasma capsulatum</em> infection. The subject received treatment with <em>amphotericin</em> <em>B deoxycholate</em> with good evolution, and outpatient management with<em> itraconazole</em>. PH is a rare entity that requires high clinical suspicion, especially in immunocompetent patients. The patient was informed that non-identifying information from the case would be submitted for publication, and he provided consent.
