The genomic sequence of the attenuated hog cholera virus Lapinized Chinese strain (HCLV) was determined from overlapping cDNA clones. The viral RNA of HCLV stain comprised 12 310 nucleotide (nt) including 374 nt and 2...The genomic sequence of the attenuated hog cholera virus Lapinized Chinese strain (HCLV) was determined from overlapping cDNA clones. The viral RNA of HCLV stain comprised 12 310 nucleotide (nt) including 374 nt and 239 nt at the 5′ and 3′-noncoding region, respectively. The complete genome sequence contained one large open reading frame which encoded an amino acid sequence of 3 898 residues with a calculated molecular weight of 437×103. Although there were mostly only small differences between the sequence of the HCLV strain and the published sequences of strains ALD, GPE?, Alfort and Brescia, there was one notable insertion of 12 nucleotides, TTTTCTTTTTTC in the 3′ non-coding region of HCLV strain.展开更多
Classical swine fever caused by Classical swine fever virus （CSFV） is a serious problem for swine industries in developing countries, which successful control of the disease have been relying on vaccination. However...Classical swine fever caused by Classical swine fever virus （CSFV） is a serious problem for swine industries in developing countries, which successful control of the disease have been relying on vaccination. However, classical swine fever still occurs in some immunized swine herds for various reasons. In this study, we conducted animal experiments to examine the influence of single or mixed infection with Porcine parvo virus （PPV）, Pseudorabies virus （PRV） and Porcine reproductive and respiratory syndrome virus （PRRSV） on the protective immunity induced by the Lapinized hog cholera virus （HCLV） vaccine and the pathogenicity of CSFV. In experiment 1, pigs were first inoculated with PPV, PRV or PRRSV, then immunized with HCLV, and finally challenged with a highly virulent CSFV Shimen strain. All of the pigs immunized with HCLV survived after the challenge, while all of the pigs in the non-immunized control group died after the challenge. The pigs in the group immunized with HCLV did not show any clinical symptoms of classical swine fever and were negative with CSFV after the challenge. The pigs infected with the non-CSFV before HCLV immunization did not display any clinical symptoms after the challenge with CSFV Shiman strain, but 11 of the 12 pigs were positive with CSFV. In experiment 2, pre-infections with PPV, PRV, and PRRSV were followed by inoculation with a low-virulence CSFV strain （CSFV 39）, and then the pigs were challenged with the CSFV Shimen strain. Infections by either PPV, PRV or PRRSV did not enhance the virulence of CSFV-39, but pigs infected by a mixture of the 3 viruses developed clinical symptoms after inoculation with CSFV-39. The mixed infection also increased mortality caused by the challenge with the CSFV Shimen strain. Together, these results showed PPV, PRV and PRRSV infections in pigs can reduce the efficacy of the HCLV vaccine and enhance the pathogenicity of CSFV, which may partly explain the immunization failure against CSFV in some swine herds.展开更多
[Objective] This paper aimed to provide scientific basis for establishing appropriate immune program. [Method] HC antibody level examinations were done for the 270 copies of serum collected from pig farm. [ Result] 10...[Objective] This paper aimed to provide scientific basis for establishing appropriate immune program. [Method] HC antibody level examinations were done for the 270 copies of serum collected from pig farm. [ Result] 106 copies of serum were found giving pre-feeding immunization, and the proportion of pigs at 20, 30, 40, 50, and 60-day old after giving pre-feeding immunization without serum immunity were 0, 0, 14.2% and 0 respectively. The proportion of antibody titer ≥ 1 ： 128 dropped from 42.6% at the age of 40 days to 28.4% at the age of 50 days and 12.5% at the age of 60 days. For the examined 164 copies of 25-day old piglets＇ serum titer, all maternal antibody was≥1 ： 16, but the proportion of antibody titer i〉 1 ： 128 dropped from 96% at the age of 20 days to 62.5% at the age of 25 days. [ Conclusion ] Diverse proportions of pigs with unqualified immunization and antibody level existed in swinery, this might be the main reason for hog cholera outbreak in pig farms展开更多
文摘The genomic sequence of the attenuated hog cholera virus Lapinized Chinese strain (HCLV) was determined from overlapping cDNA clones. The viral RNA of HCLV stain comprised 12 310 nucleotide (nt) including 374 nt and 239 nt at the 5′ and 3′-noncoding region, respectively. The complete genome sequence contained one large open reading frame which encoded an amino acid sequence of 3 898 residues with a calculated molecular weight of 437×103. Although there were mostly only small differences between the sequence of the HCLV strain and the published sequences of strains ALD, GPE?, Alfort and Brescia, there was one notable insertion of 12 nucleotides, TTTTCTTTTTTC in the 3′ non-coding region of HCLV strain.
文摘Classical swine fever caused by Classical swine fever virus （CSFV） is a serious problem for swine industries in developing countries, which successful control of the disease have been relying on vaccination. However, classical swine fever still occurs in some immunized swine herds for various reasons. In this study, we conducted animal experiments to examine the influence of single or mixed infection with Porcine parvo virus （PPV）, Pseudorabies virus （PRV） and Porcine reproductive and respiratory syndrome virus （PRRSV） on the protective immunity induced by the Lapinized hog cholera virus （HCLV） vaccine and the pathogenicity of CSFV. In experiment 1, pigs were first inoculated with PPV, PRV or PRRSV, then immunized with HCLV, and finally challenged with a highly virulent CSFV Shimen strain. All of the pigs immunized with HCLV survived after the challenge, while all of the pigs in the non-immunized control group died after the challenge. The pigs in the group immunized with HCLV did not show any clinical symptoms of classical swine fever and were negative with CSFV after the challenge. The pigs infected with the non-CSFV before HCLV immunization did not display any clinical symptoms after the challenge with CSFV Shiman strain, but 11 of the 12 pigs were positive with CSFV. In experiment 2, pre-infections with PPV, PRV, and PRRSV were followed by inoculation with a low-virulence CSFV strain （CSFV 39）, and then the pigs were challenged with the CSFV Shimen strain. Infections by either PPV, PRV or PRRSV did not enhance the virulence of CSFV-39, but pigs infected by a mixture of the 3 viruses developed clinical symptoms after inoculation with CSFV-39. The mixed infection also increased mortality caused by the challenge with the CSFV Shimen strain. Together, these results showed PPV, PRV and PRRSV infections in pigs can reduce the efficacy of the HCLV vaccine and enhance the pathogenicity of CSFV, which may partly explain the immunization failure against CSFV in some swine herds.
基金National Agricultural Achievement Reformer Program of Ministry of Science and Technology(2012GB2420045)Major Special Program of Transgenic Organism New Variety's Breeding(NO.2013ZX08006-001)Qinhuangdao Science and Technology Development Plan(NO.201101A181)
文摘[Objective] This paper aimed to provide scientific basis for establishing appropriate immune program. [Method] HC antibody level examinations were done for the 270 copies of serum collected from pig farm. [ Result] 106 copies of serum were found giving pre-feeding immunization, and the proportion of pigs at 20, 30, 40, 50, and 60-day old after giving pre-feeding immunization without serum immunity were 0, 0, 14.2% and 0 respectively. The proportion of antibody titer ≥ 1 ： 128 dropped from 42.6% at the age of 40 days to 28.4% at the age of 50 days and 12.5% at the age of 60 days. For the examined 164 copies of 25-day old piglets＇ serum titer, all maternal antibody was≥1 ： 16, but the proportion of antibody titer i〉 1 ： 128 dropped from 96% at the age of 20 days to 62.5% at the age of 25 days. [ Conclusion ] Diverse proportions of pigs with unqualified immunization and antibody level existed in swinery, this might be the main reason for hog cholera outbreak in pig farms
