[Objective] To construct prokaryotic expression vectors encoding gene Erb3binding protein （EBP1）, which plays important roles in regulating plant organ size from Nervilia fordii （Hance） Schltr. [Methods] PCR produ...[Objective] To construct prokaryotic expression vectors encoding gene Erb3binding protein （EBP1）, which plays important roles in regulating plant organ size from Nervilia fordii （Hance） Schltr. [Methods] PCR products of NfEBP1 with particular restriction sites and expression vectors, pET-28 and pET-16b were digested. Ligation, transformation and selection were performed to construct the recombinant plasmids pET-28-NfEBP1 and pET-16-NfEBP1. The recombinant plasmids were transformed into E. coli BL21 using heat -shock transformation. [Results] Recombinant plasmids pET-28-NfEBP1-1188 and pET-16-NfEBP1-1188 were constructed and transformed into expressional host cells, E. coli BL21, and validated by colony PCR, sequencing and double digestion. [Conclusion] Prokaryotic expression vectors of EBP1 gene from N. fordii were successfully constructed, which laid the foundation for characterization of the gene function.展开更多
To study the chemical constituents in Bletilla ochracea Schltr. , repeated column chromatographies and preparative TLC were used for the isolation of compounds, and spectroscopic techniques （NMR, IR, UV and MS） were...To study the chemical constituents in Bletilla ochracea Schltr. , repeated column chromatographies and preparative TLC were used for the isolation of compounds, and spectroscopic techniques （NMR, IR, UV and MS） were used for their structural identification. Seven compounds, 2,7-bis （ allyloxy ） -5-methoxy-3-methyl-9, 10-dihydrophenanthrene（1）, gastrodin（2）, gastrodigenin（3）, β-sitosterol（4）, stigmasterol（5）, 4-hydroxybernzaldehyde（6）, and daucosterol（7） were obtained from the roots of B. ochracea Schltr. Compound 1 is structurally illustrated as a novel compound, and the others are isolated from the title plant for the first time.展开更多
Hepatocellular carcinoma(HCC)remains the sixth most common malignancy and the fourth leading cause of cancer death worldwide.Numerous Chinese herbs have been used to prevent and treat HCC.Periploca forrestii Schltr.,b...Hepatocellular carcinoma(HCC)remains the sixth most common malignancy and the fourth leading cause of cancer death worldwide.Numerous Chinese herbs have been used to prevent and treat HCC.Periploca forrestii Schltr.,belonging to the family of Asclepiadaceae,has been reported to have strong inhibitory efficacy against various types of cancer cells,but the mechanism is unclear.Hence,we utilized the compound-disease target network based on the network pharmacology analysis to find out the active ingredients from P.forrestii and the potential target proteins involved in hepatocellular carcinoma.The results showed that the most effective compounds in this plant might be caffeoylquinic acids and the most potential target might be GMP synthase[glutamine-hydrolyzing](PDB:2vxo).This study is of great significance to the further research of P.forrestii and provides a novel treatment strategy for HCC.展开更多
To analyze the chemical constituents of volatile oils from the fruit of Periploca forrestii Schltr.which were collected in Guizhou province.This volatile oils were extracted by simultaneous distillation extraction (SD...To analyze the chemical constituents of volatile oils from the fruit of Periploca forrestii Schltr.which were collected in Guizhou province.This volatile oils were extracted by simultaneous distillation extraction (SDE)and microwave assistant extraction(MAE) method,and ananlyzed by gas chromatography-mass spectrometry(GC-MS)method.The main chemical compounds were methyl salicylate,β-amyrin and β-amyrin acetate etc.The compounds from the volatile oils extracted with SDE and MAE are quite different.The results provide scientific proof for the further development and utilization of Periploca forrestii Schltr.展开更多
基金Supported by Research Fund of the Doctoral Program of Higher Education (200805720004)Scientific Research Foundation for Returned Scholars, Ministry of Education of China ([2009]1001)~~
文摘[Objective] To construct prokaryotic expression vectors encoding gene Erb3binding protein （EBP1）, which plays important roles in regulating plant organ size from Nervilia fordii （Hance） Schltr. [Methods] PCR products of NfEBP1 with particular restriction sites and expression vectors, pET-28 and pET-16b were digested. Ligation, transformation and selection were performed to construct the recombinant plasmids pET-28-NfEBP1 and pET-16-NfEBP1. The recombinant plasmids were transformed into E. coli BL21 using heat -shock transformation. [Results] Recombinant plasmids pET-28-NfEBP1-1188 and pET-16-NfEBP1-1188 were constructed and transformed into expressional host cells, E. coli BL21, and validated by colony PCR, sequencing and double digestion. [Conclusion] Prokaryotic expression vectors of EBP1 gene from N. fordii were successfully constructed, which laid the foundation for characterization of the gene function.
文摘To study the chemical constituents in Bletilla ochracea Schltr. , repeated column chromatographies and preparative TLC were used for the isolation of compounds, and spectroscopic techniques （NMR, IR, UV and MS） were used for their structural identification. Seven compounds, 2,7-bis （ allyloxy ） -5-methoxy-3-methyl-9, 10-dihydrophenanthrene（1）, gastrodin（2）, gastrodigenin（3）, β-sitosterol（4）, stigmasterol（5）, 4-hydroxybernzaldehyde（6）, and daucosterol（7） were obtained from the roots of B. ochracea Schltr. Compound 1 is structurally illustrated as a novel compound, and the others are isolated from the title plant for the first time.
文摘Hepatocellular carcinoma(HCC)remains the sixth most common malignancy and the fourth leading cause of cancer death worldwide.Numerous Chinese herbs have been used to prevent and treat HCC.Periploca forrestii Schltr.,belonging to the family of Asclepiadaceae,has been reported to have strong inhibitory efficacy against various types of cancer cells,but the mechanism is unclear.Hence,we utilized the compound-disease target network based on the network pharmacology analysis to find out the active ingredients from P.forrestii and the potential target proteins involved in hepatocellular carcinoma.The results showed that the most effective compounds in this plant might be caffeoylquinic acids and the most potential target might be GMP synthase[glutamine-hydrolyzing](PDB:2vxo).This study is of great significance to the further research of P.forrestii and provides a novel treatment strategy for HCC.
文摘To analyze the chemical constituents of volatile oils from the fruit of Periploca forrestii Schltr.which were collected in Guizhou province.This volatile oils were extracted by simultaneous distillation extraction (SDE)and microwave assistant extraction(MAE) method,and ananlyzed by gas chromatography-mass spectrometry(GC-MS)method.The main chemical compounds were methyl salicylate,β-amyrin and β-amyrin acetate etc.The compounds from the volatile oils extracted with SDE and MAE are quite different.The results provide scientific proof for the further development and utilization of Periploca forrestii Schltr.
