Light is one of the key environmental factors for insects to survive.Artificial light sources different from natural environmental light can cause light stress in insects.Yellow and green light stress can interfere wi...Light is one of the key environmental factors for insects to survive.Artificial light sources different from natural environmental light can cause light stress in insects.Yellow and green light stress can interfere with the diurnal rhythm of nocturnal moths and their mating,oviposition,and adult longevity.The scarabaeid beetles Anomala corpulenta Motschulsky and Holotrichia parallela Motschulsky are widely distributed,and they are very harmful underground pests.In order to clarify the effects of light stress on their behaviors,individuals of both species were exposed to yellow light(565-585 nm)and green light(525-545 nm),with different light intensity gradients of yellow light in a laboratory setting.The short-term light stress treatment of A.corpulenta and H.parallela was carried out at night.The number of beetles emerging per half an hour was recorded,and mating pairs and feeding activity in 24 h were counted.The results showed that yellow and green light stress significantly changed the rhythm and reduced the rate of beetle emergence in the two beetle species investigated.Also,the peak emergence activity was delayed and the feeding and mating activities were significantly reduced.When treated with different intensities of yellow light,it was found that the rate of emergence of A.corpulenta under 10 lx was close to the control groups.The rate of emergence in H.parallela was significantly lower than the control groups before 0:00,in the 60 lx and 110 lx treatment groups,but after 0:00,the emergence rate of H.parallela was significantly higher in the 60 lx and 110 lx treatment groups than other treatments.However,the emergence rhythms in the three light intensity treatment groups are basically the same as in the control groups.The feeding amount and mating beetles in the three light intensity treatment groups were significantly lower than in the control groups.There were no significant differences in the three treatments.The results show that light stress above 10 lx significantly interferes with the behavioral activities of the two beetle species,investigated.This study provides a new approach for a light control technology for nocturnal beetle pest species.展开更多
昆虫病原线虫作为生物杀虫剂已被广泛应用。为了提高其对蛴螬的防治效果,筛选与昆虫病原线虫适宜混用的化学杀虫剂是一项重要的应用研究。本研究测试了70%吡虫啉水分散粒剂、40%毒死蜱乳油和2.5%高效氯氰菊酯乳油对病原线虫Heterorhabdi...昆虫病原线虫作为生物杀虫剂已被广泛应用。为了提高其对蛴螬的防治效果,筛选与昆虫病原线虫适宜混用的化学杀虫剂是一项重要的应用研究。本研究测试了70%吡虫啉水分散粒剂、40%毒死蜱乳油和2.5%高效氯氰菊酯乳油对病原线虫Heterorhabditis bacteriophora Cangzhou strain、Steinernema longicaudumX-7和Heterorhabditis indica LN2存活以及侵染率的影响;筛选出杀虫剂与病原线虫混合对暗黑鳃金龟Holotrichia parallela 2龄幼虫致死效果的最佳配比。结果显示:3种杀虫剂对3种线虫的存活均无亚致死作用;吡虫啉对3种线虫的致死率无明显影响,其中LN2在1/50 RC(含量为9.33μg mL 1)和1/100 RC(4.67μg mL 1)低浓度处理的死亡率显著低于对照;推荐浓度的高效氯氰菊酯(16.67μg mL 1)对3种线虫的致死率均显著高于对照;不同浓度的毒死蜱对3种线虫的致死率存在显著差异。70%吡虫啉1/10推荐浓度(有效成分为46.67μg mL 1)与线虫Hb混用暗黑鳃金龟2龄幼虫死亡率提高了87.51%,与X-7和LN2混用后,致死率分别提高了39.99%和47.37%。其中1/10推荐浓度吡虫啉与线虫Hb混用对暗黑鳃金龟2龄幼虫表现出增效作用,其它浓度的吡虫啉和另外两种药剂与3种线虫混用的各处理均表现为相加作用。展开更多
基金supported by the Fund for the National Natural Science Foundation of China(Grant No.32001902)Henan Scientific and Technical Attack Project(Grant No.12102110460)+2 种基金Underground pest post of the national wheat industry system(CARS-03)Major public welfare special projects in Henan Province(201300111500)Basic scientific research business fee of Henan Academy of Agricultural Sciences(2021zc42).
文摘Light is one of the key environmental factors for insects to survive.Artificial light sources different from natural environmental light can cause light stress in insects.Yellow and green light stress can interfere with the diurnal rhythm of nocturnal moths and their mating,oviposition,and adult longevity.The scarabaeid beetles Anomala corpulenta Motschulsky and Holotrichia parallela Motschulsky are widely distributed,and they are very harmful underground pests.In order to clarify the effects of light stress on their behaviors,individuals of both species were exposed to yellow light(565-585 nm)and green light(525-545 nm),with different light intensity gradients of yellow light in a laboratory setting.The short-term light stress treatment of A.corpulenta and H.parallela was carried out at night.The number of beetles emerging per half an hour was recorded,and mating pairs and feeding activity in 24 h were counted.The results showed that yellow and green light stress significantly changed the rhythm and reduced the rate of beetle emergence in the two beetle species investigated.Also,the peak emergence activity was delayed and the feeding and mating activities were significantly reduced.When treated with different intensities of yellow light,it was found that the rate of emergence of A.corpulenta under 10 lx was close to the control groups.The rate of emergence in H.parallela was significantly lower than the control groups before 0:00,in the 60 lx and 110 lx treatment groups,but after 0:00,the emergence rate of H.parallela was significantly higher in the 60 lx and 110 lx treatment groups than other treatments.However,the emergence rhythms in the three light intensity treatment groups are basically the same as in the control groups.The feeding amount and mating beetles in the three light intensity treatment groups were significantly lower than in the control groups.There were no significant differences in the three treatments.The results show that light stress above 10 lx significantly interferes with the behavioral activities of the two beetle species,investigated.This study provides a new approach for a light control technology for nocturnal beetle pest species.
文摘昆虫病原线虫作为生物杀虫剂已被广泛应用。为了提高其对蛴螬的防治效果,筛选与昆虫病原线虫适宜混用的化学杀虫剂是一项重要的应用研究。本研究测试了70%吡虫啉水分散粒剂、40%毒死蜱乳油和2.5%高效氯氰菊酯乳油对病原线虫Heterorhabditis bacteriophora Cangzhou strain、Steinernema longicaudumX-7和Heterorhabditis indica LN2存活以及侵染率的影响;筛选出杀虫剂与病原线虫混合对暗黑鳃金龟Holotrichia parallela 2龄幼虫致死效果的最佳配比。结果显示:3种杀虫剂对3种线虫的存活均无亚致死作用;吡虫啉对3种线虫的致死率无明显影响,其中LN2在1/50 RC(含量为9.33μg mL 1)和1/100 RC(4.67μg mL 1)低浓度处理的死亡率显著低于对照;推荐浓度的高效氯氰菊酯(16.67μg mL 1)对3种线虫的致死率均显著高于对照;不同浓度的毒死蜱对3种线虫的致死率存在显著差异。70%吡虫啉1/10推荐浓度(有效成分为46.67μg mL 1)与线虫Hb混用暗黑鳃金龟2龄幼虫死亡率提高了87.51%,与X-7和LN2混用后,致死率分别提高了39.99%和47.37%。其中1/10推荐浓度吡虫啉与线虫Hb混用对暗黑鳃金龟2龄幼虫表现出增效作用,其它浓度的吡虫啉和另外两种药剂与3种线虫混用的各处理均表现为相加作用。
