Determination of Mangiferin and Homomangiferin in the Leaves of 93 Kinds of Mango

[Objectives]To determine the content of mangiferin and homomangiferin in mango leaves by HPLC.[Methods]The mangiferin and homomangiferin were separated and determined by Elite Hypersil C18(5μm,4.6 mm ID×250 mm)chromatographic column.Acetonitrile-0.1%(V/V)phosphoric acid solution was used as the mobile phase for gradient elution,the flow rate was 1.0 mL/min,the detection wavelength was 258 nm,the column temperature was 30℃,and the injection volume was 5μL.[Results]There was a good linear relationship between mangiferin and homomangiferin in the range of 0.0254-0.5080μg/μL(r=0.9999)and 0.000960-0.019200μg/μL(r=0.9999),respectively.The average recovery rate(n=6)of mangiferin and homomangiferin in mango leaves was 101.7%(RSD=2.0%)and 101.0%(RSD=1.7%),respectively.[Conclusions]There were great differences in the content of mangiferin and homomangiferin in the leaves of different varieties of mango.The experimental results could provide a scientific basis for further development and utilization of mango leaf resources.

芒果苷原料药中杂质高芒果苷的含量测定

目的分析测定芒果苷原料药中高芒果苷的含量。方法采用C18柱（大连依利特分析仪器有限公司，Hypersil ODS 5μm，4．6mm×250mm），流动相为甲醇-0．1％磷酸（32：68），流速1ml／min，检测波长258nm。结果高芒果苷在0．0424-0．848μg范围内进样量与峰面积呈良好的线性关系（r=0．9999），平均回收率为97．8％，RSD=2．2％（n=6）。结论该方法简便，灵敏，重复性好，可作为芒果苷原料药中高芒果苷的含量测定方法。

一测多评法同时测定芒果止咳片中6种药效成分的含量

目的:建立同时测定芒果止咳片中没食子酸、原儿茶酸、芒果苷、高芒果苷、金丝桃苷和异槲皮苷含量的方法。方法:采用高效液相色谱法。色谱柱为Phenomenex Gemini C18,流动相为0.1%磷酸水溶液-乙腈(梯度洗脱),流速为1.0 mL/min,检测波长为258 nm,柱温为30℃,进样量为5μL。以没食子酸为内参物,计算原儿茶酸、芒果苷、高芒果苷、金丝桃苷和异槲皮苷的相对校正因子,并将一测多评法与外标法测定结果进行比较。结果:没食子酸、原儿茶酸、芒果苷、高芒果苷、金丝桃苷和异槲皮苷检测质量浓度的线性范围分别为45.75~1 830μg/m L(r=0.999 9)、2.525~101.0μg/mL(r=0.999 9)、65.33~2 613μg/m L(r=0.999 6)、9.058~362.3μg/mL(r=0.999 9)、3.885~155.4μg/mL(r=0.999 9)、1.870~74.8μg/mL(r=0.999 9);定量限分别为0.571、0.643、1.053、0.854、0.830、1.500μg/mL,检测限分别为0.171、0.193、0.316、0.256、0.249、0.450μg/mL;精密度、稳定性、重复性试验RSD均小于3%;加样回收率分别为98.8%~101.9%(RSD=1.3%,n=6)、94.3%~101.5%(RSD=3.3%,n=6)、97.9%~100.5%(RSD=0.9%,n=6)、98.2%~101.6%(RSD=1.2%,n=6)、102.3%~106.1%%(RSD=1.3%,n=6)、96.6%~99.3%(RSD=1.0%,n=6)。以没食子酸为内参物,原儿茶酸、芒果苷、高芒果苷、金丝桃苷、异槲皮苷的相对校正因子分别为0.568 3、0.500 3、0.687 6、0.939 1、0.826 3;一测多评法测得原儿茶酸等5种成分的含量范围分别为0.197~0.440、3.263~11.250、0.201~1.196、0.168~0.381、0.115~0.293 mg/片,外标法测得的含量范围分别为0.198~0.441、3.329~11.570、0.206~1.194、0.171~0.380、0.119~0.298 mg/片,两种方法测量结果的相对误差为-3.80%~0.74%,且差异无统计学意义(P>0.05)。结论:所建一测多评含量测定方法准确、可靠、重复性好,可用于同时测定芒果止咳片中6种药效成分的含量。