Background:To facilitate the preparation of traditional Chinese medicines they are pre-mashed,i.e.,mashed in advance.However,storage conditions for pre-mashed traditional Chinese medicines are based on subjective judg...Background:To facilitate the preparation of traditional Chinese medicines they are pre-mashed,i.e.,mashed in advance.However,storage conditions for pre-mashed traditional Chinese medicines are based on subjective judgments of pharmacists,and the best storage conditions have not generally been determined.Semen Zizyphi Spinosae is a commonly used traditional Chinese medicine,and it is usually used after it is fried.The medicine needs to be mashed in a timely manner to ensure its effectiveness.The Chinese Pharmacopoeia provides a limit for its aflatoxin content.Methods:The orthogonal experimental design method optimizes the best plan for pre-mashed fried Semen Zizyphi Spinosae.Experimental conditions were defined using the L^(9)(3^(4))orthogonal design table.Four factors and three levels were used to study storage conditions.The four factors and three levels are as follows:storage temperature(10°C,20°C,and 30°C),storage humidity(45%,60%,and 75%),storage times(10,20 and 30 days),and particle sizes for the powder(coarsest,coarse and medium powders).The contents of jujuboside A,spinosin,aflatoxin B1,total aflatoxins(aflatoxins B1,B2,G1,and G2),moisture,total ash,acid value,and saponification values were measured.Results:The results demonstrated that the highest jujuboside A and spinosin contents were obtained using a storage temperature of 20°C,a storage humidity of 75%,a storage time of 10 days,and with a coarse powder particle size.Aflatoxin B1 and total aflatoxins(aflatoxins B1,B2,G1 and G2)were not detected under these conditions.Conclusion:There is no requirement for traditional Chinese medicines to be pre-mashed.This study evaluated various storage conditions for pre-mashed fried Semen Zizyphi Spinosae,and considered the influence of four factors on the contents of jujuboside A,spinolin and aflatoxin for quality control to provide a reference for other pre-mashed traditional Chinese medicines.展开更多
目的:研究酸枣仁总生物碱及其环肽生物碱成分mucron ine J的抗惊厥作用。方法:采用士的宁致小鼠惊厥法,观察酸枣仁生物碱对小鼠惊厥潜伏期、死亡时间和死亡率的影响。结果:酸枣仁总生物碱50、100 mg/kg组及环肽生物碱20 mg/kg组与对照...目的:研究酸枣仁总生物碱及其环肽生物碱成分mucron ine J的抗惊厥作用。方法:采用士的宁致小鼠惊厥法,观察酸枣仁生物碱对小鼠惊厥潜伏期、死亡时间和死亡率的影响。结果:酸枣仁总生物碱50、100 mg/kg组及环肽生物碱20 mg/kg组与对照组比较,小鼠出现惊厥的时间及死亡时间均明显延长,有统计学意义(P<0.05或P<0.01)。结论:酸枣仁生物碱具有抗惊厥作用。展开更多
首次报道了补益中药酸枣仁和补骨脂中磷脂含量的红外光谱测定。以 PC 为标准品,氯仿为溶剂,P—O—C 键的吸收带为测定波长,在0.6～4.0mg/ml 范围内,吸收度与浓度呈线性关系,标准差为0.0682,CV=1.69%。与比色法测定结果比较,本法操作简便...首次报道了补益中药酸枣仁和补骨脂中磷脂含量的红外光谱测定。以 PC 为标准品,氯仿为溶剂,P—O—C 键的吸收带为测定波长,在0.6～4.0mg/ml 范围内,吸收度与浓度呈线性关系,标准差为0.0682,CV=1.69%。与比色法测定结果比较,本法操作简便,结果稳定。展开更多
比较河北、山东、河南、辽宁、陕西等13个不同产地酸枣仁中Mg、Mn、Fe、Zn、Cu、Cd、Hg和Pb微量元素的含量。采用微波消解技术处理样品,采用电感耦合等离子体质谱法(inductively coupled plasma mass spectrometry,ICPMS)测定13个不同...比较河北、山东、河南、辽宁、陕西等13个不同产地酸枣仁中Mg、Mn、Fe、Zn、Cu、Cd、Hg和Pb微量元素的含量。采用微波消解技术处理样品,采用电感耦合等离子体质谱法(inductively coupled plasma mass spectrometry,ICPMS)测定13个不同产地酸枣仁中8种微量元素的含量变化;并利用SPSS统计软件中主成分分析对测定的结果进行综合分析。通过添加标准品进行回收试验,结果表明该方法的回收率在91.85%～99.67%之间,相对标准偏差(relative standard deviation,RSD)均小于2.24%。不同产地酸枣仁中对人体有益的元素Mg、Mn、Fe、Zn含量普遍较丰富;有害的重金属元素Cu、Cd、Hg、Pb含量均在限量标准范围内。主成分分析结果显示:13个不同产地酸枣仁可分为4类,前两个主成分的总贡献率达到72.14%。ICP-MS法具有同时进行多种元素测定、准确度高等优点,该测定结果为评价市售酸枣仁品种特征提供一定的数据支撑。展开更多
基金This work was supported by the Shanghai Municipal Commission of Health and Family Planning(No.20174Y0034)the Third batch of specialized subject construction of traditional Chinese medicine in Jiading District-Traditional Chinese Medicine(No.2020-JDZYYZDXK-01).
文摘Background:To facilitate the preparation of traditional Chinese medicines they are pre-mashed,i.e.,mashed in advance.However,storage conditions for pre-mashed traditional Chinese medicines are based on subjective judgments of pharmacists,and the best storage conditions have not generally been determined.Semen Zizyphi Spinosae is a commonly used traditional Chinese medicine,and it is usually used after it is fried.The medicine needs to be mashed in a timely manner to ensure its effectiveness.The Chinese Pharmacopoeia provides a limit for its aflatoxin content.Methods:The orthogonal experimental design method optimizes the best plan for pre-mashed fried Semen Zizyphi Spinosae.Experimental conditions were defined using the L^(9)(3^(4))orthogonal design table.Four factors and three levels were used to study storage conditions.The four factors and three levels are as follows:storage temperature(10°C,20°C,and 30°C),storage humidity(45%,60%,and 75%),storage times(10,20 and 30 days),and particle sizes for the powder(coarsest,coarse and medium powders).The contents of jujuboside A,spinosin,aflatoxin B1,total aflatoxins(aflatoxins B1,B2,G1,and G2),moisture,total ash,acid value,and saponification values were measured.Results:The results demonstrated that the highest jujuboside A and spinosin contents were obtained using a storage temperature of 20°C,a storage humidity of 75%,a storage time of 10 days,and with a coarse powder particle size.Aflatoxin B1 and total aflatoxins(aflatoxins B1,B2,G1 and G2)were not detected under these conditions.Conclusion:There is no requirement for traditional Chinese medicines to be pre-mashed.This study evaluated various storage conditions for pre-mashed fried Semen Zizyphi Spinosae,and considered the influence of four factors on the contents of jujuboside A,spinolin and aflatoxin for quality control to provide a reference for other pre-mashed traditional Chinese medicines.
文摘目的:研究酸枣仁总生物碱及其环肽生物碱成分mucron ine J的抗惊厥作用。方法:采用士的宁致小鼠惊厥法,观察酸枣仁生物碱对小鼠惊厥潜伏期、死亡时间和死亡率的影响。结果:酸枣仁总生物碱50、100 mg/kg组及环肽生物碱20 mg/kg组与对照组比较,小鼠出现惊厥的时间及死亡时间均明显延长,有统计学意义(P<0.05或P<0.01)。结论:酸枣仁生物碱具有抗惊厥作用。
文摘比较河北、山东、河南、辽宁、陕西等13个不同产地酸枣仁中Mg、Mn、Fe、Zn、Cu、Cd、Hg和Pb微量元素的含量。采用微波消解技术处理样品,采用电感耦合等离子体质谱法(inductively coupled plasma mass spectrometry,ICPMS)测定13个不同产地酸枣仁中8种微量元素的含量变化;并利用SPSS统计软件中主成分分析对测定的结果进行综合分析。通过添加标准品进行回收试验,结果表明该方法的回收率在91.85%～99.67%之间,相对标准偏差(relative standard deviation,RSD)均小于2.24%。不同产地酸枣仁中对人体有益的元素Mg、Mn、Fe、Zn含量普遍较丰富;有害的重金属元素Cu、Cd、Hg、Pb含量均在限量标准范围内。主成分分析结果显示:13个不同产地酸枣仁可分为4类,前两个主成分的总贡献率达到72.14%。ICP-MS法具有同时进行多种元素测定、准确度高等优点,该测定结果为评价市售酸枣仁品种特征提供一定的数据支撑。
