Methods and Effects of Hongshan Cock Spermatozoa Cryopreservation

The survival rates, acrosomic integrity rates of frozen semen from Hongshan cock and fertilities of the frozen-thawed spermatozoa in both pellets and straws were studied. The result shows that the average survival rate of spermatozoa in straws was 0, 451±0, 056, and 0. 390±0. 040 in pellets, the former was significantly higher than the later （n=20, p〈0, 01）, The average acrosomic integrity rate of the sperms was 0, 613±0. 049 in straws, and 0. 476±0. 057 in pellets. The former was significantly higher than the later （n= 20, p〈0.05）. The fertility rates （%） of sperms in pellets, straws and freshly colleered semen at the third day after artificial insemination （AI） were 85.32±2. 32, 87. 73±1.00 and 90.77±1.68, respectively. The fertility rate,s of the three types of semen at the fourth and fifth days were lower than the third day＇s. Variance analysts shows that the fertilities of the spermatozoa in pellets and straws were significantly lower than freshly collected semen （n=3, p〈0.01）, and the fertilities of the spermatozoa in straws were significantly higher than those in pellets （n= 3, p〈0.01）.

洪山公鸡精液冷冻保存方法及效果的研究

研究比较了两种不同类型的冷冻精液在解冻后精子活率、顶体完整率以及受精率方面的差异。实验结果表明,细管型冷冻精液解冻后的活率显著高于颗粒型(n=20,p<0.01)。细管型冷冻精液解冻后的顶体完整率显著高于颗粒型(n=20,p<0.05)。受精实验结果表明,两种类型的冷冻精液的受精率都显著低于以新鲜精液输精的对照组(p<0.01),颗粒型冷冻精液的受精率显著高于细管型冷冻精液(p<0.01)。