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Optimization of Honing Wheel Structure Parameters in Ultra-precision Plane Honing
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作者 SYOJI Katsuo 《厦门大学学报(自然科学版)》 CAS CSCD 北大核心 2002年第S1期57-58,共2页
Free abrasive particle machining in simple machine such as: honing, polishing can get higher surface finish mirror, but surface error, and working procedure is hard to control. Therefore, the vertical disposed ultra-p... Free abrasive particle machining in simple machine such as: honing, polishing can get higher surface finish mirror, but surface error, and working procedure is hard to control. Therefore, the vertical disposed ultra-precision plane honing method by ultra-particle diamond honing wheel is put forward to. The results of experiments indicate: plane-honing wheel has higher machining accuracy and machining efficiency. But at the same time the structure parameters of honing wheel effects on machining accuracy. By analyzing the relation of honing wheel structure parameters and workpiece machining accuracy, the relation of honing wheel and wear coefficient, then this paper gets honing wheel structure parameters in the condition of best accuracy coefficient and wear coefficient, and resolve the problem of choosing honing wheel structure parameters in ultra-precision plane honing at last. This paper analyses the relation of honing wheel structure parameters and workpiece machining accuracy coefficient and wear coefficient, by building relative movement math model of honing wheel and workpiece in plane honing. Through theory calculating, the result indicate: about honing machine tools for large volume manufacture, honing wheel wear is main effect factor, so honing wheel should adopt obverse triangle radial structure. About honing machining for high accuracy and low-batch quantities, machining accuracy coefficient is main factors; so honing wheel should adopt reverse triangle radial structure. Neglected the manufacturing factors of honing wheel, then we can design honing wheel with high power curve structure to meet the need of machining accuracy coefficient and honing wheel wear coefficient in higher accuracy honing. 展开更多
关键词 ultra-precision plane honing honing wheel structure machining accuracy optimization parameters
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INFLUENCE OF WHEEL STRUCTURAL PARAMETERS ON MACHINING ACCURACY OF ULTRA-PRECISION PLANE HONING 被引量:4
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作者 Guo Yinbiao Department of Mechanical and Electrical Engineering, Xiamen University, Xiamen 361005, ChinaHu Jianyu Zheng Xiaoguang Katsuo SyojiXiamen University Chongqing University Tohoku University, Japan 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2002年第4期344-347,共4页
A new idea for designing wheel patterns is presented so as to solve theproblems about machining accuracy of workpiece and wear of honing wheel in ultra-precision planehoning. The influence factors on motion principle ... A new idea for designing wheel patterns is presented so as to solve theproblems about machining accuracy of workpiece and wear of honing wheel in ultra-precision planehoning. The influence factors on motion principle and pattern structures are analyzed andoptimization machining parameters are obtained. By calculating effective cutting length on thesurface of workpiece cut by wheel's abrasive and the orbit of one point on the surface of workpiececontacting with wheel, the wear coefficient of different kinds of wheels and accuracy coefficient ofworkpiece machined by corresponding wheels are obtained. Furthermore, the simulation results showthat the optimal pattern structure of wheel turns out to have lower wheel wear and higher machiningaccuracy. 展开更多
关键词 fine grit diamond wheel ultra-precision plane honing machining accuracy wheel wear
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The Experimental and Theoretical Research of "Local Resonance" in Ultrasonic Honing System 被引量:1
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作者 ZHU Xun-sheng, XU Ke-wei, ZHAO Bo, MA Deng-zhe (Mechanical School of Shanghai Jiaotong University, Shanghai 200030, China) 《厦门大学学报(自然科学版)》 CAS CSCD 北大核心 2002年第S1期49-,共1页
In 1982, Professor Fang Guoliang found the "Non full resonance" phenomenon in a tool system while he use the thin-long tool ultrasonically machining deep-small hole. He called it as "local resonance&quo... In 1982, Professor Fang Guoliang found the "Non full resonance" phenomenon in a tool system while he use the thin-long tool ultrasonically machining deep-small hole. He called it as "local resonance". Also this "Non full resonance" phenomenon was discovered in the ultrasonic drilling and the ultrasonic honing system later. To its mechanism, professor Fang thought that the coupling of long-thin tool bar and driving system is weak, so the tool bar can vibrate independently, but the quantitative relation between the coupling factor and diameter ratio is not made certain. Then several theories come forth to interpret it but still haven’t a common conclusion. Through the systematic experimental and theoretical research, this paper reveals that the "local resonance" phenomenon of ultrasonic honing system has the same essence with the "local resonance" phenomenon in deep hole machining system: when the section area ratio of tool bar and driving system is small enough, some resonance frequencies of combined system are close to the resonance frequencies of "fixed-free" state tool bar, the combined system is still resonant. According to the given depth of hole and structure size, we can use the transfer matrix deduced in this paper to design flexible bar and oilstone seat not only satisfying mechanical structure size but also achieving enough magnitude. It greatly simplified the design. This new method can be named as "local resonance" design method for ultrasonic honing system. The experiment, deduction and design method have a certain common meaning to the study and design of other ultrasonic system. 展开更多
关键词 local resonance ultrasonic honing system full resonance transfer matrix method
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Analysis of Material Removal in Alumina Ceramic Honing
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作者 YU Ai-bing 1, XU Yan-shen 1, CHEN Si-fu 1, LIN Bin 1, TIAN Xin-li 2 (1. The State Education Ministry Key Laboratory of High Temperature Structure Ceramics & Machining Technology for Engineering Ceramics, Tianjin University, Tianjin 300072, China 2. Institute of Armored Force, Beijing 100072, China) 《厦门大学学报(自然科学版)》 CAS CSCD 北大核心 2002年第S1期51-52,共2页
The removal mechanism is of importance to the grinding of hard and brittle ceramic materials. It is more suitable to analyze the material removal during ceramics honing processes by means of indention fracture approac... The removal mechanism is of importance to the grinding of hard and brittle ceramic materials. It is more suitable to analyze the material removal during ceramics honing processes by means of indention fracture approach. There are two honing characteristics different from grinding, the honing incidental tensile stresses and the crosshatch pattern. The stresses may influence material removal of brittle ceramics with lower tensile strength. In addition, the criss-cross cutting pattern on a bore known as crosshatch may also have its effect on the material removal of ceramics. The material removal of alumina is analyzed on the basis of honing characteristics, honing incidental tensile stresses and crosshatch pattern. By means of indentation fracture mechanics of brittle solids, the theoretical analysis of stress intensity factor and the crack response prove that honing incidental tensile stresses can increase the stress intensity factor of honing cracks and decrease the grinding stresses. So, the fracture criterion for crack propagation can be met easily. Therfore, it is possible to machine ceramic materials with small grinding forces in honing processes. The alumina honing experiments show that material at crosshatch intersecting point is removed by way of chipping which is similar to the edge-crumbled of ceramics. For brittle ceramics with lower tensile strength, such as Al 2O 3, SiO 2, the influences of the honing incidental tensile stresses and the crosshatch pattern on material removal are bigger than that of ceramics with relative higher toughness, such as ZrO 2 and Si 3N 4. Hence, the honing of Al 2O 3, SiO 2, is superior in cutting ability to the grinding. The large stock removal of brittle ceramic materials can be obtained through higher honing pressures. The increase in honing pressures can increase intensity factor of honing cracks, decrease the grinding stresses, and remarkably improve material removal rate. The researches show that honing is an efficient bore machining operation for brittle ceramics. 展开更多
关键词 material removal honing CERAMICS stresses
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Research on the New Technology and System of Ultrasonic Honing
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作者 ZHU Xi-jing 1,2, XU Hong-jun 1, WANG Ai-ling 2, SHENG Xin-quan 2 (1. Nanjing University of Aeronautics and Astronautics, Nanjing 210006, China 2. North China Institute of Technology, Taiyuan 030051, China) 《厦门大学学报(自然科学版)》 CAS CSCD 北大核心 2002年第S1期66-67,共2页
In recent years, the new technology of ultrasonic vibration cutting has been drawing general attention. And great advancement in theory and applications in production of the technology have been achieved. Ultrasonic v... In recent years, the new technology of ultrasonic vibration cutting has been drawing general attention. And great advancement in theory and applications in production of the technology have been achieved. Ultrasonic vibration honing system developed in North China Institute of Technology and Nanjing University of Aeronautics and Astronautics of cast iron cylinder (47×97) and thin cylinder of 20# (86×95) has been successfully applied to productive experiment and the technological effect obtained is ideal. In comparison with ordinary honing, ultrasonic vibration honing possesses apparent characteristics: Firstly, it has high processing precision (precision in size ranks IT6, roughness Ra0.05, circularty and cylindericity in size ranks IT6); Secondly, it has small cutting force and low temperature so that no deformation occurs; Thirdly, it has high efficiency. In this paper, the application of a new technique to honing is introduced, and the ultrasonic honing systems of thin cylinder and cast cylinder which have been successfully manufactured and their applying effect are illustrated. This paper analyzes the cutting principle of ultrasonic honing in terms of cutting motion, grain performance and cutting quantity. The study of this technique starts a new approach to which is efficient higher quality and lower consumption to hole’s precise and superprecise machining. At last,the paper introduce the conclution: Ultrasonic honing sharpens grain, which is favorable for raising cutting efficiency; Ultrasonic vibration honing may increase honing depth and raise cutting efficiency. It may also decrease honing power and system deformation; Undergoing practice we can find that the ultrasonic honing system as above discribed can be extended and applied to production. The system not only can be applied to conventional honing machine, its technology effect as good as high precision machine tool, but also can be applied to develop and study ultrasonic vibrating numbrical controlling honing machine tool which will provide a new type, efficient, high quality low consumption, equipment for working precision hole; Appling the same parament as W40 oilstone bar, under the same condition, is very valuable for product and application. Rough machining and finish machining once time finished, which not only improve location accuracy, but also have a high effiency.The honing system not only can be suitable for honing cast cylinder, but also can be applied for honing engineer ceramics as well as hard, crisp material; The dimension and technology paraments of 47 mm honing system as above discribed, can be suitable for honing the cast cylinder of motorcycle or minicar. And the cylinder diameter can be randomly selected from 45 to 65. The dimension and technology paramagnets of 86 honing system as above discribed,can be applied for honing all kinds of cylinder of light or heavy passenger-cargo vehicle, and the cylinder diameter can be randomly selected from 65 to 100; It is easy to achieve new technology of flat roof honing by applying ultrasonic honing.After the flat roof honing, fuel consumption of engine is 1g/Hp, while conventional engine is 3g/Hp.Life expectancy of engine is prolonged greatly after the cylinder by flat roof honing. 展开更多
关键词 ultrasonic honing cutting principle CYLINDER applying effect
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Honing Farming Skills
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作者 Li Xiaoyu 《ChinAfrica》 2017年第6期32-33,共2页
ANIMAL husbandry technician Vasco Silva always found it difficult to master the technology applied to artificial insemination (AI) of cattle. However, in December last year. a training course changed the Mozambican... ANIMAL husbandry technician Vasco Silva always found it difficult to master the technology applied to artificial insemination (AI) of cattle. However, in December last year. a training course changed the Mozambican's original thought of the technology and the way it is used, 展开更多
关键词 honing Farming Skills
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Non-Resonant Structure Design of Catenary Transformer in Ultrasonic Gear Honing 被引量:8
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作者 LI Xiangpeng ZHANG Chunhui WANG Shiying 《Wuhan University Journal of Natural Sciences》 CAS 2012年第1期67-72,共6页
To solve the design problem of transformer composed of non-resonant structure in ultrasonic gear-honing, force coupling conditions for moderately thick annular plate (MTAP) and catenary horn are proposed, and the fr... To solve the design problem of transformer composed of non-resonant structure in ultrasonic gear-honing, force coupling conditions for moderately thick annular plate (MTAP) and catenary horn are proposed, and the frequency equations of the transformer, which consist of an MTAP and a catenary horn, are derived based on Mindlin's theory. The design parameters of the transformer were obtained by solving the frequency equations with the help of MATLAB, and its mode and frequency were deduced by the modal analysis of finite element method (FEM), which are consistent with the theoretical design demands. The transformer design can be extended from system with thin annular plate to that with MTAP as the theoretical method by the simulation analysis of various ratios of the thickness to radius. 展开更多
关键词 ultrasonic gear honing moderately thick annular plate (MTAP) Mindlin's theory catenary transformer
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Characterization and life prediction of single-pass honing tool for fuel injection nozzle 被引量:1
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作者 Changyong YANG Hao SU +4 位作者 Shaowu GAO Qingfeng AI Yucan FU Wenfeng DING Jiuhua XU 《Chinese Journal of Aeronautics》 SCIE EI CAS CSCD 2021年第4期225-240,共16页
Single-pass honing is an important machining method for finish machining of holes,which can meet the requirement for high efficiency and consistency of holes.Characterization and life prediction of single-pass honing ... Single-pass honing is an important machining method for finish machining of holes,which can meet the requirement for high efficiency and consistency of holes.Characterization and life prediction of single-pass honing tool are necessary to improve the machining accuracy of holes honed,especially dimension accuracy.Single-pass honing tool is a single layer abrasive tool with fixed dimension,which still remains problematic for characterization and life prediction.For fuel injection nozzles with bore diameter under 1 mm,the stiffness of the single-pass honing tool is poor.This article presents a novel analytical model that predicts life of the tool with poor stiffness.Firstly,according to the bore diameter and dimension tolerance,the single-pass honing tool is designed and manufactured.Based on the prepared single-pass honing tool,the measurement and characterization methods are established.Furthermore,the tool wear tests are carried out,and the tool contour evolution model is established to predict the tool life. 展开更多
关键词 Fuel injection nozzle Life prediction Single-pass honing tool Tool contour evolution Tool stiffness
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一类具有Holling I型反应函数半比例依赖的捕食-食饵离散系统的全局稳定性
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作者 李晓红 赵建涛 王晓霞 《科技通报》 北大核心 2013年第9期1-3,共3页
主要研究了一类具有Holling I型功能函数半比例依赖的捕食-食饵离散系统的全局稳定性,同时给出了该系统存在一个全局稳定的正周期解的充分条件。
关键词 离散 honing I型 周期解 全局稳定
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具有HollingⅢ类功能性反应的基于比率的离散Leslie捕食者—食饵模型的持久性
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作者 吴润莘 林丽玉 《福建工程学院学报》 CAS 2006年第6期841-844,共4页
利用差分方程比较原理探讨了周期情形下一类具有HollingⅢ类功能性反应的基于比率的离散Leslie捕食者—食饵模型的持久性,得到了这种模型持久性的充分条件。
关键词 honing Ⅲ类功能性反应 基于比率 捕食 食饵 持久性
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具有Holling III类功能反应的三维食物链差分系统周期解的存在性
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作者 刘兴臻 《广州大学学报(自然科学版)》 CAS 2006年第5期5-9,共5页
利用重合度理论研究了一类具有Holling III类功能反应及时滞的三维食物链差分系统全局周期解的存在性,得到了该系统存在严格正周期解的充分条件.
关键词 食物链差分系统 honing Ⅲ类功能反应 正周期解 时滞 重合度理论
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捕食者与被捕食者问题的定性分析
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作者 刘翠桃 《河南科学》 2009年第9期1044-1046,共3页
对于一类具有简化HollingIV类功能性反应的扑食与食饵模型做了较为详细的定性分析,得到了系统轨线的全局稳定性,极限环的存在性及系统无环的一些充分条件.部分地改进了文献[2]的一些结果.
关键词 捕食者与被捕食者 简化honing Ⅳ类功能性反应 定性分析
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人鼻咽癌细胞系HONE1的蛋白质双向凝胶电泳分析 被引量:9
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作者 李峰 关勇军 +4 位作者 谢锦云 何春梅 陈平 陈主初 梁宋平 《中国癌症杂志》 CAS CSCD 2001年第4期294-298,共5页
目的 :研究人鼻咽癌细胞系HONE1的蛋白质表达特征。方法 :抽提鼻咽癌细胞系HONE1总蛋白 ,双向凝胶电泳 (2 DE)分离、银染显色 ,用凝胶成像仪和PDQUEST软件获取HONE1总蛋白的 2 DE图像 ;根据获得的 2 DE凝胶图像 ,从胶上切取分辨良好... 目的 :研究人鼻咽癌细胞系HONE1的蛋白质表达特征。方法 :抽提鼻咽癌细胞系HONE1总蛋白 ,双向凝胶电泳 (2 DE)分离、银染显色 ,用凝胶成像仪和PDQUEST软件获取HONE1总蛋白的 2 DE图像 ;根据获得的 2 DE凝胶图像 ,从胶上切取分辨良好、染色较浓的蛋白质点 ,用胰蛋白酶原位水解 ,所获酶解肽段经基质辅助激光解吸飞行时间质谱 (MALDI TOF MS)测定Mr;以测得的肽段Mr为肽质量指纹 (PMF) ,通过PepIdent软件搜索SWISS PROT和TrEMBL数据库而识别该点对应的蛋白质。结果 :建立了双向凝胶电泳分离总蛋白、质谱鉴定银染蛋白质点的方法 ,并鉴定出人鼻咽癌细胞系HONE1中表达较强的 10个点相应的蛋白质。结论 :本研究为建立人鼻咽癌细胞HONE1的 2 DE参考图和蛋白质数据库提供了有用的基础性研究资料 ,为进一步识别鼻咽癌特异性的蛋白质奠定了实验基础。 展开更多
关键词 鼻咽癌 细胞系 双向凝胶电泳 蛋白质 鉴定 HONE1
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miR-516b-5p靶向ATM调控鼻咽癌细胞HONE1 DNA修复的机制研究 被引量:2
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作者 张勇 张炜 范崇盛 《实用癌症杂志》 2021年第4期527-531,共5页
目的探讨鼻咽癌细胞HONE1中抑制DNA修复的潜在机制。方法使用ETP处理鼻咽癌细胞HONE1造成DNA损伤后,通过高通量测序检测鼻咽癌细胞HONE1中miRNA的表达谱。过表达差异miRNA后,通过基于I-PpoI的可诱导DSB系统检测DNA双链断裂处(DNA double... 目的探讨鼻咽癌细胞HONE1中抑制DNA修复的潜在机制。方法使用ETP处理鼻咽癌细胞HONE1造成DNA损伤后,通过高通量测序检测鼻咽癌细胞HONE1中miRNA的表达谱。过表达差异miRNA后,通过基于I-PpoI的可诱导DSB系统检测DNA双链断裂处(DNA double-strand breaks,DSB)的DNA-PKcs含量。通过miRDB在线分析miRNA的潜在靶标。通过荧光素酶报告系统检测miRNA靶向mRNA。结果ETP处理后hsa-miR-9983-3p、hsa-miR-4731-5p、hsa-miR-3675-5p、hsa-miR-516b-5p、hsa-miR-4437、hsa-miR-7114-5p、hsa-miR-637、hsa-miR-5008-5p的表达水平上升至少8倍。miR-516b-5p mimics导致DSB的DNA-PKcs含量减少(P<0.05),而miR-516b-5p inhibitor导致DSB的DNA-PKcs含量增加(P<0.05)。在鼻咽癌细胞HONE1中过表达miR-516b-5p后,ATM mRNA的表达水平和蛋白表达水平下降,DNA-PKcs的磷酸化水平下降;而抑制miR-516b-5p表达后,ATM mRNA的表达水平和蛋白表达水平上升,DNA-PKcs的磷酸化水平上升。同时,miR-516b-5p靶向ATM的3端非编码区。过表达ATM后,DSB的DNA-PKcs含量上升(P<0.05),而敲低ATM后,DSB的DNA-PKcs含量下降(P<0.05)。结论miR-516b-5p通过靶向ATM mRNA的3端非编码区,降解了ATM mRNA,抑制了ATM的翻译,随后DNA-PKcs的磷酸化水平下降,DNA-PKcs与DSB的结合减少,最终抑制了鼻咽癌细胞HONE1的DNA修复。 展开更多
关键词 miR-516b-5p ATM 鼻咽癌 HONE1 DNA修复
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Brutal Honesty
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作者 Margaux Schreurs 《China's Foreign Trade》 2014年第3期92-92,共1页
In reality,it is easier and better to be honest.One of my favourite,and least favourite characteristics of my Chinese friends is that they are extremely honest.In the West,we spend a lot of time being extremely care-f... In reality,it is easier and better to be honest.One of my favourite,and least favourite characteristics of my Chinese friends is that they are extremely honest.In the West,we spend a lot of time being extremely care-ful what we say to each other,and we also like to make sure that we are always 展开更多
关键词 EXTREMELY FRIENDS REALITY SPEND REALLY Brutal Hone
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具有非线性密度制约的HollingⅡ类功能性反应的食饵与捕食者模型的定性分析 被引量:11
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作者 邱树林 吴承强 陈江彬 《福州大学学报(自然科学版)》 CAS CSCD 北大核心 2006年第1期14-18,共5页
研究一类具有非线性密度制约的HollingⅡ型功能性反应的食饵-捕食者系统:x′=xg(x)-yφ(x),y′=y(-d+kφ(x))在g(x)=a-b x的情况下.分析了该系统的平衡点性态,证明了系统在正平衡点的外围极限环的存在性,得出了在一定的条件下,正平衡点... 研究一类具有非线性密度制约的HollingⅡ型功能性反应的食饵-捕食者系统:x′=xg(x)-yφ(x),y′=y(-d+kφ(x))在g(x)=a-b x的情况下.分析了该系统的平衡点性态,证明了系统在正平衡点的外围极限环的存在性,得出了在一定的条件下,正平衡点外围至少有2或者3个极限环的结论. 展开更多
关键词 平衡点 极限环 食饵-捕食者 honing
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海带多糖和茶多酚对鼻咽癌细胞HONE1和CNE2增殖的影响 被引量:4
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作者 孙文忠 魏媛媛 +2 位作者 曾曼丽 郑实兴 徐志文 《临床耳鼻咽喉头颈外科杂志》 CAS 北大核心 2013年第8期425-428,共4页
目的:观察海带多糖(LJP)和茶多酚(TP)单独及联合应用对鼻咽癌(NPC)细胞HONE1和CNE2增殖的影响,了解LJP对体内移植瘤的抑制作用,探讨其抗癌机制。方法:以NPC细胞HONE1和CNE2为研究对象,绘制两者生长曲线,计算倍增时间确定对数生长期细胞... 目的:观察海带多糖(LJP)和茶多酚(TP)单独及联合应用对鼻咽癌(NPC)细胞HONE1和CNE2增殖的影响,了解LJP对体内移植瘤的抑制作用,探讨其抗癌机制。方法:以NPC细胞HONE1和CNE2为研究对象,绘制两者生长曲线,计算倍增时间确定对数生长期细胞,采用MTT检测LJP和TP单独及联合应用对两组细胞的增殖抑制率,运用Annexin V加PI双染法检测LJP对HONE1细胞凋亡的影响;以人NPC细胞株HONE1建立裸鼠皮下移植瘤模型及行体内抑瘤实验。结果:LJP和TP均对两组细胞有增殖抑制作用,LJP和TP联合应用对两组实验细胞抑制作用均高于两者单独应用的效果,且这种作用呈浓度依赖关系。流式细胞仪检测结果显示,LJP具有诱导HONE1细胞凋亡的作用,凋亡率随着LJP浓度的增加而增高,320mg/L的凋亡率为(49.51±1.89)%(P<0.01)。体内实验中,25.0mg/kg LJP组、50.0mg/kg LJP组的抑瘤率分别为33.7%(P<0.05)和47.0%(P<0.01),具有明显抑制移植瘤的作用,而12.5mg/kg LJP组的抑瘤率仅为16.4%(P>0.05)。结论:LJP和TP对鼻咽癌细胞HONE1和CNE2增殖均有抑制作用,两者联合应用更具有显著效果,LJP作用机制可能是通过诱导癌细胞凋亡而实现的。 展开更多
关键词 海带多糖 茶多酚 HONE1细胞 CNE2细胞 四甲基偶氮唑蓝检测法 鼻咽肿瘤
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具有改进的Leslie-Grower式的Holling-Tanner模型的稳定性分析
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作者 纪丽丽 吴承强 《福州大学学报(自然科学版)》 CAS CSCD 北大核心 2009年第6期771-775,797,共6页
研究一个具有改进的Leslie-Grower式的Holling-Tanner模型.分析了该系统的平衡点性态,利用Dulac函数证明了系统在正平衡点外围不存在极限环,从而证明了正平衡点在第一象限内是全局渐近稳定的.
关键词 honing—Tanner模型 Leslie—Grower DULAC函数 极限环
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冬凌草甲素影响细胞外排功能增加HONE1细胞对米托蒽醌的敏感性 被引量:1
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作者 潘俊成 邓嘉茵 +3 位作者 胡杨 刘丹阳 王妍 黄奕俊 《热带医学杂志》 CAS 2017年第5期557-559,587,F0003,共5页
目的观察非毒性剂量冬凌草甲素是否增加鼻咽癌细胞株HONE1对米托蒽醌的敏感性。方法采用MTT法使用不同浓度的冬凌草甲素处理HONE1细胞株,确定冬凌草甲素的非毒剂量区间;然后用非毒性剂量冬凌草处理细胞后用流式细胞术进行侧群表型检测,... 目的观察非毒性剂量冬凌草甲素是否增加鼻咽癌细胞株HONE1对米托蒽醌的敏感性。方法采用MTT法使用不同浓度的冬凌草甲素处理HONE1细胞株,确定冬凌草甲素的非毒剂量区间;然后用非毒性剂量冬凌草处理细胞后用流式细胞术进行侧群表型检测,探索能显著降低侧群表型的冬凌草甲素浓度;参考冬凌草甲素非毒性剂量和能降低侧群表型的剂量得到冬凌草甲素用于联用米托蒽醌的浓度。结果 5μmol/L冬凌草甲素并未对HONE1细胞产生明显毒性,该浓度处理细胞24 h后,细胞侧群比例由3.32%降低至0.55%;米托蒽醌联用5μmol/L冬凌草甲素组HONE1细胞存活率较单用米托蒽醌组低(P<0.05);流式细胞术分析显示膜外排蛋白ABCG2含量降低。结论冬凌草甲素可以通过影响细胞ABCG2外排功能增加鼻咽癌细胞株HONE1对米托蒽醌的敏感性。 展开更多
关键词 冬凌草甲素 HONE1 米托蒽醌
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水果词汇大拼盘
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作者 骆家隆 《初中生之友(快乐号)(上)》 2008年第12期48-48,共1页
前两天,同事拿来一袋名叫papaya的东西,尝了尝,香香甜甜,很好吃。继而一想,这好吃的东西到底是什么啊?翻开词典,答案揭晓,原来是"木瓜"。随即得出结论:需要重新认识水果家族的成员。今天,为大家准备了一个"水果大拼盘&qu... 前两天,同事拿来一袋名叫papaya的东西,尝了尝,香香甜甜,很好吃。继而一想,这好吃的东西到底是什么啊?翻开词典,答案揭晓,原来是"木瓜"。随即得出结论:需要重新认识水果家族的成员。今天,为大家准备了一个"水果大拼盘",甜的、酸的、热带的、冬天的、夏天的,应有尽有。不过可别学我,要知道名字在先,吃在后哦! 展开更多
关键词 PAPAYA MELON LONGAN 葡萄籽 血橙 杏肉 ORANGE 蓝莓 LEMON hone
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