目的阐明藏药长毛风毛菊的药效及化学成分,探讨其有效成分、关键靶点和相关通路,明确其治疗肾性水肿的作用机制。方法将SD大鼠分为空白组、阿霉素诱导肾性水肿模型组,长毛风毛菊给药低剂量组、高剂量组。测定血清生化指标,探讨各组总蛋...目的阐明藏药长毛风毛菊的药效及化学成分,探讨其有效成分、关键靶点和相关通路,明确其治疗肾性水肿的作用机制。方法将SD大鼠分为空白组、阿霉素诱导肾性水肿模型组,长毛风毛菊给药低剂量组、高剂量组。测定血清生化指标,探讨各组总蛋白(total protein,TP)、尿素氮(blood u-rea nitrogen,BUN)、血肌酐(serum creatinine,SCr)、甘油三酯(triglyceride,TG)、总胆固醇(serum total cholestero,TC)、低密度脂蛋白(low density lipoprotein,LDL-C)、高密度脂蛋白(high density lipopro-tein,HDL-C)指标的情况。采用UPLC-Q-TOF-MS/MS技术对藏药长毛风毛菊的化学成分进行分析;在SwissTargetPrediction、GeneCards数据库分别获取长毛风毛菊化学成分靶点和肾性水肿靶点,取交集获得治疗肾性水肿作用靶点;通过STRING数据库进行蛋白互作分析;利用Metascape平台进行gene ontology(GO)功能和京都基因和基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路分析;将药物、靶点等导入到Cytoscape3.7.2构建“药物-成分-靶点-疾病-通路”网络图。筛选出排名靠前的6个基因和12个有效成分,通过分子对接技术验证网络药理预测的准确性,揭示长毛风毛菊治疗肾性水肿的作用机制。结果大鼠血清中TP、HDL-C指标上调,BUN、SCr、TC、TG和LDL-C指标均下调。从藏药长毛风毛菊提取物中共鉴定了71个化合物,包括7个苯丙素类、16个绿原酸类和39个黄酮类等成分。通过预测得出75个长毛风毛菊治疗肾性水肿靶点,GO富集共涉及生物过程、细胞组分、分子功能3个方面,KEGG富集结果显示PI3K/AKT、AGE-RAGE、MAPK等是显著通路。分子对接结果显示,有效成分与关键靶点结合能Vina评分均小于0,高效验证了网络药理预测的准确性。结论该研究基于药效学实验,结合UPLC-Q-TOF-MS/MS鉴定、网络药理学分析和分子对接技术,表明长毛风毛菊能够治疗肾性水肿,可能通过调控PI3K/AKT、AGE-RAGE、MAPK信号通路中TP53、PI3K、AKT等相关靶点来发挥作用。展开更多
The physical properties and chemical components of three oil samples extracted from Illicium verum Hook. f. by steam distillation （SD）, solvent extraction （SE） and supercritical fluid extraction （SFE） were compa...The physical properties and chemical components of three oil samples extracted from Illicium verum Hook. f. by steam distillation （SD）, solvent extraction （SE） and supercritical fluid extraction （SFE） were compared with one another and analyzed by Gas Chromatography-Mass Spectrometry （GC-MS）. The quality parameters of star anise essential oil from SFE were close to that of those came from SD and SE. Although the extraction yield of star anise by SFE （9.2 %） was close to the value from SE （9.3%）, it was yet higher than that came from SD （8.2%）. For sensory evaluation, however, three oils were significantly different. The odor and taste of the products from SFE and SE were generally more natural and vivid than that came from distilled oil. The volatile compound revealed that significant differences of the composition existed in the distilled oil and the oleoresins prepared by SFE and SE.展开更多
Safety of a compound Lonicera rupicola Hook. f. et & Thomson injection was evaluated by local irritation experiments including conjunctiva,skin and muscle irritant experiments,and the effect on weight gain was stu...Safety of a compound Lonicera rupicola Hook. f. et & Thomson injection was evaluated by local irritation experiments including conjunctiva,skin and muscle irritant experiments,and the effect on weight gain was studied. The results showed that the compound L. rupicola Hook. f. et & Thomson injection had no irritation to rabbit eyes and weak irritation to rabbit ears,and had relatively strong irritation to the leg muscles of the mice. Its metabolism in mice had no abnormal toxicity.展开更多
文摘目的阐明藏药长毛风毛菊的药效及化学成分,探讨其有效成分、关键靶点和相关通路,明确其治疗肾性水肿的作用机制。方法将SD大鼠分为空白组、阿霉素诱导肾性水肿模型组,长毛风毛菊给药低剂量组、高剂量组。测定血清生化指标,探讨各组总蛋白(total protein,TP)、尿素氮(blood u-rea nitrogen,BUN)、血肌酐(serum creatinine,SCr)、甘油三酯(triglyceride,TG)、总胆固醇(serum total cholestero,TC)、低密度脂蛋白(low density lipoprotein,LDL-C)、高密度脂蛋白(high density lipopro-tein,HDL-C)指标的情况。采用UPLC-Q-TOF-MS/MS技术对藏药长毛风毛菊的化学成分进行分析;在SwissTargetPrediction、GeneCards数据库分别获取长毛风毛菊化学成分靶点和肾性水肿靶点,取交集获得治疗肾性水肿作用靶点;通过STRING数据库进行蛋白互作分析;利用Metascape平台进行gene ontology(GO)功能和京都基因和基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路分析;将药物、靶点等导入到Cytoscape3.7.2构建“药物-成分-靶点-疾病-通路”网络图。筛选出排名靠前的6个基因和12个有效成分,通过分子对接技术验证网络药理预测的准确性,揭示长毛风毛菊治疗肾性水肿的作用机制。结果大鼠血清中TP、HDL-C指标上调,BUN、SCr、TC、TG和LDL-C指标均下调。从藏药长毛风毛菊提取物中共鉴定了71个化合物,包括7个苯丙素类、16个绿原酸类和39个黄酮类等成分。通过预测得出75个长毛风毛菊治疗肾性水肿靶点,GO富集共涉及生物过程、细胞组分、分子功能3个方面,KEGG富集结果显示PI3K/AKT、AGE-RAGE、MAPK等是显著通路。分子对接结果显示,有效成分与关键靶点结合能Vina评分均小于0,高效验证了网络药理预测的准确性。结论该研究基于药效学实验,结合UPLC-Q-TOF-MS/MS鉴定、网络药理学分析和分子对接技术,表明长毛风毛菊能够治疗肾性水肿,可能通过调控PI3K/AKT、AGE-RAGE、MAPK信号通路中TP53、PI3K、AKT等相关靶点来发挥作用。
基金Supported by the Science and Technology Key Project of Guangxi Province (0424008-1C)
文摘The physical properties and chemical components of three oil samples extracted from Illicium verum Hook. f. by steam distillation （SD）, solvent extraction （SE） and supercritical fluid extraction （SFE） were compared with one another and analyzed by Gas Chromatography-Mass Spectrometry （GC-MS）. The quality parameters of star anise essential oil from SFE were close to that of those came from SD and SE. Although the extraction yield of star anise by SFE （9.2 %） was close to the value from SE （9.3%）, it was yet higher than that came from SD （8.2%）. For sensory evaluation, however, three oils were significantly different. The odor and taste of the products from SFE and SE were generally more natural and vivid than that came from distilled oil. The volatile compound revealed that significant differences of the composition existed in the distilled oil and the oleoresins prepared by SFE and SE.
基金Supported by Project of Science and Technology Department of Sichuan Province,China(2016KZ0007)Innovative Scientific Research Project for Postgraduates of Southwest Minzu University(CX2018SZ44)
文摘Safety of a compound Lonicera rupicola Hook. f. et & Thomson injection was evaluated by local irritation experiments including conjunctiva,skin and muscle irritant experiments,and the effect on weight gain was studied. The results showed that the compound L. rupicola Hook. f. et & Thomson injection had no irritation to rabbit eyes and weak irritation to rabbit ears,and had relatively strong irritation to the leg muscles of the mice. Its metabolism in mice had no abnormal toxicity.
