A cDNA clone encoding a vacuolar H+-pyrophosphatase (V-H+-PPase) was isolated from Hordeum brevisubulatum (Trin.) Link by using RACE method. Sequence analysis revealed that HbVP1 contained 2 319 nucleotides of open re...A cDNA clone encoding a vacuolar H+-pyrophosphatase (V-H+-PPase) was isolated from Hordeum brevisubulatum (Trin.) Link by using RACE method. Sequence analysis revealed that HbVP1 contained 2 319 nucleotides of open reading frame (ORF) and 420 nucleotides of 3′-untranslated region. Its encoding protein consisted of 773 amino acid residues, which includes 14 transmembrane helices. The predicated molecular mass is 80.4 kDa with pI of 4.90. The V-H+-PPases in higher plants shared low identity (40-55%) with those of protozoa, marine alga and archaebacteria. HbVP1 transcripts accumulated abundantly in roots, shoots and seeds, and it was also strongly induced by salt treatment.展开更多
[ Objective] This study was to explore the inheritance of BC1 F3 to excellent characteristics of parents（ Elymus dahuricus, Hordeum brevisubu/atum）, heterosis and fertility restoration. E Methodl The agronomic chara...[ Objective] This study was to explore the inheritance of BC1 F3 to excellent characteristics of parents（ Elymus dahuricus, Hordeum brevisubu/atum）, heterosis and fertility restoration. E Methodl The agronomic characters such as growth period, growth rate, pollen fertility, fecundity, reproducibility and fresh grass output of Elymus dahudcus, Hordeum brevisubu/atum and BC1 F3 were comparatively studied. E Result l The growth dynamic of the BC1 F3 lined tended to like that of its parent Hordeum brevisubu/atum; although growing periods of various lines were different, they were close to that of Hordeurn brevisubulatum. There were differences in pollen fertility and seed setting, the pollen fertility rate of YF3-93 was higher than recurrent parent Hordeum brevisubulatum, its seed setting rate in open pollination was also higher. Some lines had low pollen fertility rate and seed setting rate, such as the pollen fertility rate of PF3-52. There were significant differences in the output among BC1 F3 lines, e.g. the heterosis （HP） of YF3-64, YF3-74 and YF3-83 were 75.53%, 75.12% and 66.16%, respectively; however, the yields of PF3- 52, PF3-15 and PF3-42 were lower than their parents. ~ Conclusion] This study provided reference for breeding new varieties of forage which is suitable to drought and saline environment.展开更多
[ Objective] To study the germination characteristics of Hordeum brevisubulatum seeds in the Songnen Plains of China under different conditions and find out the optimum conditions for germination. [ Method] A total of...[ Objective] To study the germination characteristics of Hordeum brevisubulatum seeds in the Songnen Plains of China under different conditions and find out the optimum conditions for germination. [ Method] A total of five variable temperatures including 15/25, 20/30, 15/30, 20/25 and 15/20 ℃, five concentrations of sodium chloride solutions including 0, 75, 85, 95 and 105 mmol/L, and five concentrations of CS2 strain （the A values were 0, 0.500 0, 0.050 0, 0.005 0 and 0.000 5, respectively） were studied by orthogonal experimental design. [ Result] The germination rate, germination energy and germination index were significantly （ P 〈 0.05） affected by the temperature and sodium chloride concentration, but rarely affected by the CS2 strain concentration. Moreover, the germination rate, germination potential and germination index were all decreased with the increasing of sodium chloride concentration. [ Conclusion] The optimal germination conditions of Hordeum brevisubulatum are as follows： temperatures of 15/25 ℃ and 15/30℃, 0 mmoVL of sodium chloride concentration.展开更多
基金This work was supported by the National Natural Science Foundation of China(30471229)
文摘A cDNA clone encoding a vacuolar H+-pyrophosphatase (V-H+-PPase) was isolated from Hordeum brevisubulatum (Trin.) Link by using RACE method. Sequence analysis revealed that HbVP1 contained 2 319 nucleotides of open reading frame (ORF) and 420 nucleotides of 3′-untranslated region. Its encoding protein consisted of 773 amino acid residues, which includes 14 transmembrane helices. The predicated molecular mass is 80.4 kDa with pI of 4.90. The V-H+-PPases in higher plants shared low identity (40-55%) with those of protozoa, marine alga and archaebacteria. HbVP1 transcripts accumulated abundantly in roots, shoots and seeds, and it was also strongly induced by salt treatment.
文摘[ Objective] This study was to explore the inheritance of BC1 F3 to excellent characteristics of parents（ Elymus dahuricus, Hordeum brevisubu/atum）, heterosis and fertility restoration. E Methodl The agronomic characters such as growth period, growth rate, pollen fertility, fecundity, reproducibility and fresh grass output of Elymus dahudcus, Hordeum brevisubu/atum and BC1 F3 were comparatively studied. E Result l The growth dynamic of the BC1 F3 lined tended to like that of its parent Hordeum brevisubu/atum; although growing periods of various lines were different, they were close to that of Hordeurn brevisubulatum. There were differences in pollen fertility and seed setting, the pollen fertility rate of YF3-93 was higher than recurrent parent Hordeum brevisubulatum, its seed setting rate in open pollination was also higher. Some lines had low pollen fertility rate and seed setting rate, such as the pollen fertility rate of PF3-52. There were significant differences in the output among BC1 F3 lines, e.g. the heterosis （HP） of YF3-64, YF3-74 and YF3-83 were 75.53%, 75.12% and 66.16%, respectively; however, the yields of PF3- 52, PF3-15 and PF3-42 were lower than their parents. ~ Conclusion] This study provided reference for breeding new varieties of forage which is suitable to drought and saline environment.
基金The authors would like to acknowledge funding from the National High Technology Research and Development Program of China (863 Program) (2008AA10Z224)National Natural Science Foundation of China (30700055,30960261)Doctoral Foundation of Ministry of Education of China (20070200005)
文摘[ Objective] To study the germination characteristics of Hordeum brevisubulatum seeds in the Songnen Plains of China under different conditions and find out the optimum conditions for germination. [ Method] A total of five variable temperatures including 15/25, 20/30, 15/30, 20/25 and 15/20 ℃, five concentrations of sodium chloride solutions including 0, 75, 85, 95 and 105 mmol/L, and five concentrations of CS2 strain （the A values were 0, 0.500 0, 0.050 0, 0.005 0 and 0.000 5, respectively） were studied by orthogonal experimental design. [ Result] The germination rate, germination energy and germination index were significantly （ P 〈 0.05） affected by the temperature and sodium chloride concentration, but rarely affected by the CS2 strain concentration. Moreover, the germination rate, germination potential and germination index were all decreased with the increasing of sodium chloride concentration. [ Conclusion] The optimal germination conditions of Hordeum brevisubulatum are as follows： temperatures of 15/25 ℃ and 15/30℃, 0 mmoVL of sodium chloride concentration.
