In order to screen the appropriate culture condition for the differentiation and regeneration of Prtmus avium L. adventitious buds, in this study, the effect of different hormone proportions on differentiation and reg...In order to screen the appropriate culture condition for the differentiation and regeneration of Prtmus avium L. adventitious buds, in this study, the effect of different hormone proportions on differentiation and regeneration of shoot tip explants were investigated using Gisela No. 5 and Gisela No. 6 as experimental materi- als. The results showed that, different hormone proportions had extremely significant effects （ P 〈0.01 ） on the differentiation rate of P. avium adventitious buds; the appropriate hormone proportions for Gisela No. 5 and Gisela No. 6 to induce dedifferentiation of adventitious buds were 6-BA 3.0 mg/L ＋ IBA 0.5 mg/L ＋ KT O. 1 mg/L and 6-BA 1.0 mg/L ＋ IBA 0.5 mg/L ＋ KT 0.2 rag/L, respectively. In addition, different hormone proportions had extremely significant effects （P 〈 0.01 ） on the regeneration coefficient and regeneration rate of P. avium adventitious buds; with the hormone proportion of 6-BA 1.0 mg/L ＋ IBA 1.0 mg/L ＋ KT 0.3mg/L, the number of regenerated adventitious buds reached the maximum for both varieties.展开更多
基金Supported by Special Fund for Key Laboratory of Shaanxi Provincial Scienceand Technology Department(2011HBSZS003)
文摘In order to screen the appropriate culture condition for the differentiation and regeneration of Prtmus avium L. adventitious buds, in this study, the effect of different hormone proportions on differentiation and regeneration of shoot tip explants were investigated using Gisela No. 5 and Gisela No. 6 as experimental materi- als. The results showed that, different hormone proportions had extremely significant effects （ P 〈0.01 ） on the differentiation rate of P. avium adventitious buds; the appropriate hormone proportions for Gisela No. 5 and Gisela No. 6 to induce dedifferentiation of adventitious buds were 6-BA 3.0 mg/L ＋ IBA 0.5 mg/L ＋ KT O. 1 mg/L and 6-BA 1.0 mg/L ＋ IBA 0.5 mg/L ＋ KT 0.2 rag/L, respectively. In addition, different hormone proportions had extremely significant effects （P 〈 0.01 ） on the regeneration coefficient and regeneration rate of P. avium adventitious buds; with the hormone proportion of 6-BA 1.0 mg/L ＋ IBA 1.0 mg/L ＋ KT 0.3mg/L, the number of regenerated adventitious buds reached the maximum for both varieties.
