A simple and efficient method for cloning the flanking genomic sequences of a known DNA region is reported in this study. This method combined partial restriction endonuclease digestion, adaptor ligation, and a single...A simple and efficient method for cloning the flanking genomic sequences of a known DNA region is reported in this study. This method combined partial restriction endonuclease digestion, adaptor ligation, and a single round polymerase chain reaction. Total genomic DNA was partially digested with the frequent-cutting restriction enzyme Mse I. The partially digested products were ligated to an unphosphorylated adaptor. A hot start PCR amplification with Taq polymerase and dNTP was performed with a DNA-specific primer and the adaptor primer complementary to the adaptor and the Mse I recognition site. The amplified products were fractionated, cloned and sequenced. By this method, we cloned the downstream region of a gynoecious marker TG/CAC234 from cucumber （Cucumis sativus L.）.展开更多
When an aircraft moves under a low carrier-to-noise ratio （CNR） or at a high speed, increasing the sensitivity of global navigation satellite system （GNSS） receiver is a goal quite hard to achieve. A novel acquisi...When an aircraft moves under a low carrier-to-noise ratio （CNR） or at a high speed, increasing the sensitivity of global navigation satellite system （GNSS） receiver is a goal quite hard to achieve. A novel acquisition scheme assisted with micro-electro-mechanical-sensor （MEMS） inertial navigation system （INS） is presented to estimate the Doppler caused by user dynamics relative to each satellite ahead of time. Based on tightly coupled GNSS/INS estimation algorithm, MEMS INS Doppler error that can be achieved is first described. Then, by analyzing the mean acquisition time and signal detection probability, the MEMS INS-assisted acquisition capabilities in cold, warm and hot starts are quantitatively determined and compared with the standard GNSS acquisition capability. The simulations and comparisons have shown that： the acquisition time in cold start can be shortened by at least 23 s, the time in warm start can be shortened to i s and the acquisition capability is improved 95%, and the reaequisition time in hot start can be shortened by around 0.090 s and the capability can be enhanced 40%. The results demonstrate the validity of the novel method.展开更多
基金partially supported by the Program 30470120,30671419,30700541 from the National Natural Science Foundation of Chinaby the 863 Programs 2006AA10Z108,2006AA100108 from the Ministry of Science and Technology of China+2 种基金by the Ph.D Funding 20050307009 from the Ministry of Education of Chinaby the Program BK2006139 from the Natural Science Foundation of Jiangsu Provinceby Research Fund KJ05006 from Nanjing Agricultural University,China.
文摘A simple and efficient method for cloning the flanking genomic sequences of a known DNA region is reported in this study. This method combined partial restriction endonuclease digestion, adaptor ligation, and a single round polymerase chain reaction. Total genomic DNA was partially digested with the frequent-cutting restriction enzyme Mse I. The partially digested products were ligated to an unphosphorylated adaptor. A hot start PCR amplification with Taq polymerase and dNTP was performed with a DNA-specific primer and the adaptor primer complementary to the adaptor and the Mse I recognition site. The amplified products were fractionated, cloned and sequenced. By this method, we cloned the downstream region of a gynoecious marker TG/CAC234 from cucumber （Cucumis sativus L.）.
基金the National High Technology Research and Development Program (863) of China(No.2009AA12Z322)
文摘When an aircraft moves under a low carrier-to-noise ratio （CNR） or at a high speed, increasing the sensitivity of global navigation satellite system （GNSS） receiver is a goal quite hard to achieve. A novel acquisition scheme assisted with micro-electro-mechanical-sensor （MEMS） inertial navigation system （INS） is presented to estimate the Doppler caused by user dynamics relative to each satellite ahead of time. Based on tightly coupled GNSS/INS estimation algorithm, MEMS INS Doppler error that can be achieved is first described. Then, by analyzing the mean acquisition time and signal detection probability, the MEMS INS-assisted acquisition capabilities in cold, warm and hot starts are quantitatively determined and compared with the standard GNSS acquisition capability. The simulations and comparisons have shown that： the acquisition time in cold start can be shortened by at least 23 s, the time in warm start can be shortened to i s and the acquisition capability is improved 95%, and the reaequisition time in hot start can be shortened by around 0.090 s and the capability can be enhanced 40%. The results demonstrate the validity of the novel method.
