Patients with AIDS(Acquired Immunodeficiency Srudrome) have immunoldgic abnormalities resulting from functional defects in HIV(Human Immunodeficiency Virus)-infected CD4+lymphocytes and monocytes. Tumor necrosis facto...Patients with AIDS(Acquired Immunodeficiency Srudrome) have immunoldgic abnormalities resulting from functional defects in HIV(Human Immunodeficiency Virus)-infected CD4+lymphocytes and monocytes. Tumor necrosis factor-alpha(TNF-α)is known to mediate cachexcia.Todetermine if TNF is responsible for 'wasting syndrome' in AIDS.we studied serum levels and in vitro production of TNF-alpha in 21 HIV-seropositive men(10 AIDS,2 ARC, 9 HIV+)and 8 HIV seronreative controls.Both a bloassay and TNF-specific ELISA were used.All patiend except onebad undctectable levels of serum TNF. while control sera contained 4.2±2.5μg/L of TNF,Peripheral blood mononuclear cell(PBMNC)was tested for spontaneous,LPS-induced or PHA and PMA-induced TNF production at different times in culture(24-72h).Patients cells Produced significantlyless TNF than normal PBMNC under all experimental conditions:e.g,18-45μg/L in conttols vs.0.4～13μg/L in patients(P<0.001)at 72 hours using PHA and PMA as activators.Cell viabilitywas 85% in all cultures.No significant differences in TNF release were detected between AIDS patients with cachexia or opportunistic infectious and other HIV-seropositive men.This study showedthat most HIV-infected persons had no dctectable serum TNF-αand that their PBMNC produced subnormal quantities of this cytoklne in vitro,suggesting that TNF is not involved in the pathogenesis ofthe cacbexia associated with AIDS.展开更多
文摘Patients with AIDS(Acquired Immunodeficiency Srudrome) have immunoldgic abnormalities resulting from functional defects in HIV(Human Immunodeficiency Virus)-infected CD4+lymphocytes and monocytes. Tumor necrosis factor-alpha(TNF-α)is known to mediate cachexcia.Todetermine if TNF is responsible for 'wasting syndrome' in AIDS.we studied serum levels and in vitro production of TNF-alpha in 21 HIV-seropositive men(10 AIDS,2 ARC, 9 HIV+)and 8 HIV seronreative controls.Both a bloassay and TNF-specific ELISA were used.All patiend except onebad undctectable levels of serum TNF. while control sera contained 4.2±2.5μg/L of TNF,Peripheral blood mononuclear cell(PBMNC)was tested for spontaneous,LPS-induced or PHA and PMA-induced TNF production at different times in culture(24-72h).Patients cells Produced significantlyless TNF than normal PBMNC under all experimental conditions:e.g,18-45μg/L in conttols vs.0.4～13μg/L in patients(P<0.001)at 72 hours using PHA and PMA as activators.Cell viabilitywas 85% in all cultures.No significant differences in TNF release were detected between AIDS patients with cachexia or opportunistic infectious and other HIV-seropositive men.This study showedthat most HIV-infected persons had no dctectable serum TNF-αand that their PBMNC produced subnormal quantities of this cytoklne in vitro,suggesting that TNF is not involved in the pathogenesis ofthe cacbexia associated with AIDS.
