Genetic Polymorphism in GDF8 Region of HU Sheep Based on Microsatellite DNA

[ Objective ] This study was to analyze the genetic polymorphism in GDF8 Region of HU sheep. [Method] Four microsatellite loci including BMS1591, TEXAN-2, FCB128 and BM81124 mapped on GDF8 region of chromosome No. 2 of sheep that may be correlated with growth performance were chosen to detect the molecular genetics foundation of growth performance of Hu sheep. [ Result] Four microsatellite loci detected were high in heterozygosity, more in effective alleles number and rich in polymorphic information, all the three indices passed through the high polymorphic level （PIC 〉0.5）. [ Conclusion ] The four microsatellite loci detected could be used to estimate the genetic polymorphism of growth performance of Hu sheep.

Relationship Between the mRNA Expression Level of TGF-β Receptor Genes in Tissues and Ovulation Rate in Hu Sheep

Eight ewes of Hu sheep which bred multi-lamb were used as the high-fecundity group and the other eight ewes of Hu sheep which bred single lamb were used as the control group to investigate the relationship between the mRNA expression level of TGF-β receptor genes in tissues and ovulation rate in Hu sheep. Cloprostenol sodium was injected to make the synchronization of estrus treatment, then all ewes were slaughtered within 24-36 h after empathema and the ovaries were collected. Furthermore, the number of ovulation points was counted to determine ovulation rate for each sheep. Tissue expression analysis was conducted by RT-PCR for one ewe form the high-fecundity group and the relationship between the mRNA expression of genes encoding TGF-β receptors and ovulation rate was detected by real-time fluorescent quantitative PCR. The results showed that the relative expression level of TGF-flR I gene in the reproductive organ was significantly higher than in the lung and muscle （P 〈 0.01）, while relative expression level of TGF-βR H in reproductive organ was significantly higher than that of other tissues （P 〈 0.01）, indicating that these are highly expressed genes in the ovary. In addition, mRNA expression level of TGF-βR I and TGF-flRH in the ovaries of the high-fecundity group were significantly higher （P〈 0.01） and obviously higher （P= 0.011） than the control group, respectively. The mRNA expression level of TGF-βR I and TGF-βR H had a positive correlation with ovulation rate and the correlation coefficients were 0.562 （P〉 0.05） and 0.711 （P〈 0.05）, respectively. It is suggested that TGF-β receptors have close relationship with highfecundity in Hu sheep.

Behavioral Observations on Introduced Hu Sheep in Henan Province,China

Hu Sheep is a local sheep breed in the plain of Taihu, China. Due to the natural conditions in Taihu and human domestication, Hu Sheep is gradually featured with high adaptability, fast growth rate, early maturity and high productivity, which make them to be a unique and rare breed of sheep. In the present study, an experiment was conducted by means of instantaneous scan sampling, focal animal sampling and all-occurrence sampling to learn about Hu Sheep's behavior and activity pattern under certain feeding management and ecological condition. The result showed that the activity time budgets for several behaviors were different between rams and ewes, with respect to feeding (rams 30.60% vs. ewes 33.23%), ruminating (33.43% vs.35.30%); sleeping (19.92% vs. 13.69%), standing (4.13% vs. 5.26%), moving (8.10% vs. 7.46%), and other behaviors (3.86% vs.5.06%). A circadian activity rhythm was shown in some behaviors; e.g., total feeding time was greater in the daytime than at night, whereas resting time showed the opposite tendency. Moving mostly occurred in the daytime, and ruminating mainly occurred at night. Additionally, feeding activity occurred 32.22 times per day in rams and 31.73 times per day in ewes, whereas ruminating activity after feeding occurred 19.83 times per day in rams and 24.34 times per day in ewes. For each food bolus,the average ruminating time was 51.22 s in rams and 49.52 s in ewes, with 68.22 bouts of rumination per day in rams and57.35 bouts of rumination in ewes. By conducting the experiment, we studied and grasped the local Hu Sheep's main behavior and regularity which are of great significance on improving the feeding management and reducing diseases.

The Fashion Detachment of Women on Runway——Hu Sheguang and His Fashion Show

On March 25th, our designer Hu Sheguang, with an unconstrained yet quite unique style, presented creative patterns during a fashion show for another new season. He made use of various uniform elements and with the fashion detachment of women, brought us a charming show. The models for the show were selected from all over the world.They wore fitted military green uniforms;exuding dominance from head to toe.Hu fully integrated Chinese and Western elements while using fur, tulle, taffeta and other materials to create a stunning astehtic.

Whole-genome resequencing of Hu sheep identifies candidate genes associated with agronomic traits

The phenotypic diversity resulting from artificial or natural selection of sheep has made a significant contribution to human civilization.Hu sheep are a local sheep breed unique to China with high reproductive rates and rapid growth.Genomic selection signatures have been widely used to investigate the genetic mechanisms underlying phenotypic variation in livestock.Here,we conduct whole-genome sequencing of 207 Hu sheep and compare them with the wild ancestors of domestic sheep(Asiatic mouflon)to investigate the genetic characteristics and selection signatures of Hu sheep.Based on six signatures of selection approaches,we detect genomic regions containing genes related to reproduction(BMPR1B,BMP2,PGFS,CYP19,CAMK4,GGT5,and GNAQ),vision(ALDH1A2,SAG,and PDE6B),nervous system(NAV1),and immune response(GPR35,SH2B2,PIK3R3,and HRAS).Association analysis with a population of 1299 Hu sheep reveals that those missense mutations in the GPR35(GPR35 g.952651 A>G;GPR35 g.952496 C>T)and NAV1(NAV1 g.84216190 C>T;NAV1 g.84227412 G>A)genes are significantly associated(P<0.05)with immune and growth traits in Hu sheep,respectively.This research offers unique insights into the selection characteristics of Hu sheep and facilitates further genetic improvement and molecular investigations.

Single‑cell sequencing reveals the reproductive variations between primiparous and multiparous Hu ewes

Background In the modern sheep production systems,the reproductive performance of ewes determines the economic profitability of farming.Revealing the genetic mechanisms underlying differences in the litter size is important for the selection and breeding of highly prolific ewes.Hu sheep,a high-quality Chinese sheep breed,is known for its high fecundity and is often used as a model to study prolificacy traits.In the current study,animals were divided into two groups according to their delivery rates in three consecutive lambing seasons(namely,the high and low reproductive groups with≥3 lambs and one lamb per season,n=3,respectively).The ewes were slaughtered within 12 h of estrus,and unilateral ovarian tissues were collected and analyzed by 10×Genomics single-cell RNA sequencing.Results A total of 5 types of somatic cells were identified and corresponding expression profiles were mapped in the ovaries of each group.Noticeably,the differences in the ovary somatic cell expression profiles between the high and low reproductive groups were mainly clustered in the granulosa cells.Furthermore,four granulosa cell subtypes were identified.GeneSwitches analysis revealed that the abundance of JPH1 expression and the reduction of LOC101112291 expression could lead to different evolutionary directions of the granulosa cells.Additionally,the expression levels of FTH1 and FTL in mural granulosa cells of the highly reproductive group were significantly higher.These genes inhibit necroptosis and ferroptosis of mural granulosa cells,which helps prevent follicular atresia.Conclusions This study provides insights into the molecular mechanisms underlying the high fecundity of Hu sheep.The differences in gene expression profiles,particularly in the granulosa cells,suggest that these cells play a critical role in female prolificacy.The findings also highlight the importance of genes such as JPH1,LOC101112291,FTH1,and FTL in regulating granulosa cell function and follicular development.

胸部CT椎体HU值在2型糖尿病骨质疏松症机会性筛查中的价值

背景:有研究表明基于腰椎CT的HU(hounsfield units)值可以筛查骨质疏松症,而目前因肺部感染就诊的患者增加,肺部感染合并2型糖尿病的患者也随之增加,增加了胸部CT的使用率。目的:探讨胸部CT检查中L_1椎体HU值在2型糖尿病骨质疏松症筛查中的作用。方法:回顾性分析2020年6月至2022年6月成都医学院第一附属医院收治的244例2型糖尿病患者的临床资料。利用双能X射线骨密度仪获得骨密度T值,按WHO的骨质疏松症诊断标准,将研究对象分为非骨质疏松组(n=120)及骨质疏松组(n=124),比较两组患者一般情况、骨密度T值及胸部CT检查中L_(1)椎体的HU值,分析HU值与各部位T值的关系并评估2型糖尿病骨质疏松症的准确性。结果与结论:(1)两组患者性别、年龄、体质量指数、糖化血红蛋白、平均血糖、钙、磷、2型糖尿病患病时间、高血压病史、高脂血症病史之间对比差异无显著性意义(P>0.05);(2)HU值与最低T值呈正相关(r=0.619,P<0.01),与髋部T值呈正相关(r=0.584,P<0.01),与股骨颈T值呈正相关(r=0.641,P<0.01);当HU值取98时,其预测2型糖尿病骨质疏松症具有良好的准确性,敏感性为70.8%;(3)提示基于胸部CT检查的L_1椎体HU值对2型糖尿病患者的骨质疏松症筛查具有良好的价值,可作为2型糖尿病骨质疏松症一种机会性、无成本的补充筛查方法。

抗Hu抗体对胃肠动力障碍性疾病神经系统损伤机制的研究进展

越来越多的研究提示靶向神经元抗体特别是抗Hu抗体可对中枢神经系统和(或)肠神经系统造成不同程度的损伤,影响相关疾病的发生、发展和预后,因此明确这类抗体对神经系统损伤的机制及其与疾病的相关性对个体化诊断和治疗具有重要指导意义。本文就抗Hu抗体与副肿瘤性胃肠动力障碍性疾病、慢性假性肠梗阻、肠易激综合征等疾病的相关性及其对神经元的损伤机制作一综述。

Bai Hu Ren Shen Soup promotes autophagy in the pancreas tissue of diabetic rats by increasing the level of autophagy protein LC3 and decreasing the level of autophagy protein p62

Objective:To investigate the therapeutic effect of Bai Hu Ren Shen Soup(BHRSS)on type 2 diabetes mellitus(T2DM)rats and explore whether its therapeutic effect is achieved by promoting autophagy in the pancreas tissue of T2DM rats.Methods:Thirty male standard deviation rats were equally divided into normal control group,T2DM group and BHRSS group.Firstly,the therapeutic effect of BHRSS on T2DM was evaluated by biochemical indices in serum and pathological changes in the pancreas tissue of rats in each group after modeling and medication.Then,the levels of microtubule-associated protein 1 light chain 3(LC3)protein and p62 protein in the pancreas tissue of rats in each group after BHRSS intervention were detected by Western blot.Results:BHRSS intervention significantly reduced the levels of blood sugar,liver and kidney function,and other related biochemical indices in the serum of T2DM rats,and effectively improved the pathological changes in the pancreas tissue of T2DM rats.In addition,Western blot results showed that the expression levels of autophagy-related protein LC3 protein in the pancreas tissue of rats were increased,and the expression level of p62 protein was decreased after BHRSS intervention.Conclusion:BHRSS has a clear therapeutic effect on T2DM model rats,which may be related to that increase of autophagy protein LC3 and the decrease of p62 protein level in the pancreas tissue.

基于Hu不变矩和最佳缝合线的纸箱垛图像拼接

为了实现对港口集装箱中纸箱垛的快速定位与拆垛,需要获得纸箱垛最外层清晰的全景图像。基于自主研制的自动卸货车,采用两摄像头多轮次单程拍摄的方法获取待拼接图像,在单轮次图像拼接中引入Hu不变矩寻找参考图像和待配准图像之间最佳重叠区域,并使用优化的动态规划方法搜索最佳缝合线,以此快速获得清晰的大视野图像。试验表明,基于Hu不变矩和最佳缝合线的全景图像拼接在特征点匹配上减少20%以上的时间,并可以较好地消除因拍摄角度造成的鬼影。

基于Shearlet变换和Hu矩的零水印算法

数字水印技术被广泛应用于版权保护,为了更好地实现版权保护,增强水印算法的鲁棒性与不可感知性,兼顾几何攻击与非几何攻击的抵抗能力,提出一种基于Shearlet变换和Hu矩的零水印算法.对载体图像进行Shearlet变换和分块离散余弦变换,并分块计算Hu矩以构建特征矩阵,进而构造零水印.构造零水印前使用Kent混沌映射加密,保障了安全性.在仿真实验中对不同纹理的图像进行常见的高强度单一攻击以及组合攻击.结果表明,归一化互相关数值均高于0.99,峰值信噪比均高于75.80.该水印方案有完美的不可感知性,且能够抵御高强度的常见攻击及组合攻击,具有较强的鲁棒性.

Characteristics of Xiao Chai Hu decoction based on randomized controlled trials: A bibliometric analysis

Objective:To explore the characteristics of Xiao Chai Hu(XCH)decoction in randomized controlled trials(RCTs)using a bibliometric method.Methods:Seven databases including PubMed,Web of Science,Embase,Cochrane library,China National Knowledge Infrastructure(CNKI),Chinese Scientific Journal Database(VIP)and Wanfang database were retrieved from inception to May 27,2022.In the study,XCH decoction(or modified)used alone or combined with conventional Western medicine as an intervention measure was included.The basic characteristics,funding support,relevant diseases,intervention methods,and adverse events(AEs)were analyzed.Data analysis was performed using SPSS 26.0 software.Results:A total of 813 RCTs were included,published from 1989 to 2022.There was only one Englishlanguage literature with the recent impact factor of 5.374.There were 147 studies were from Chineselanguage core journals,with the highest impact factor of 2.414.Only 6.15%of the literatures mentioned funding support.96.31%of the included literature reported the statistical significance of using XCH decoction.The diseases treated mainly included chronic hepatitis B(9.35%),cough variant asthma(5.66%),dizziness(5.54%),bile reflux gastritis(4.43%),and fever(4.18%).However,the overall research design of the included literature was poor,and large sample size,multicenter RCTs are needed.The incidence of AEs of XCH decoction alone was 8.86%,which was significantly lower than that of conventional Western medicine treatment.The combination of XCH decoction and conventional Western medicine treatment could reduce the incidence of AEs,and no serious adverse event was reported.Conclusion:Although the included studies show that XCH decoction is widely used,and has good efficacy and few AEs.Due to the low quality of the included RCTs,there may be some bias,and its rational use based on the specific conditions is recommended in clinical practice.

基于Hu不变矩和径向基神经网络的太阳镜镜片瑕疵图像分类系统

现有的镜片瑕疵分类主要针对光学玻璃镜片和树脂镜片,与太阳镜镜片的生产原料和工艺存在差异,导致两者的瑕疵类型也有所不同,因此现有的镜片检测机不能直接应用于太阳镜镜片的检测.为了满足生产需求,实现工业自动化检测,本文利用基于机器视觉检测技术的CMOS传感器工业相机、双远心镜头、LED灯搭建了镜片图像采集系统,并融合图像处理算法,将七个Hu矩不变量作为互补特征,采用径向基神经网络模型开发了太阳镜镜片瑕疵图像分类系统.实验表明,使用Hu不变矩特征提取算法的分类方法可有效提高分类准确率,该方法的准确率为94.56%.和BP等其他神经网络结构相比,准确率也更高.因此,该系统被证明是可行和有效的.

基于数理统计特征的人机交互图像手势识别

在人机交互领域中手势识别的应用前景十分广阔,在诸多领域中均为人类带来了无限便利。基于数理统计特征设计一种人机交互图像手势识别方法,实现了基于数理统计特征的手势信息获取与基于识别结果的人机交互。对人机交互图像实施图像灰度化处理、二值化处理、平滑处理、边缘检测和轮廓提取处理等一系列预处理。通过OpenCV提取二值化人机交互图七个具有尺度不变性、旋转不变性、平移不变性的Hu矩,前四个矩描述手势的图像椭圆、主轴方向角、面积、旋转半径这四个物理量,后三个矩描述的是图像对称性、重心、中心距。基于Darknet-19改进YOLO-V2网络的骨干网络,使模型能够预测的anchor box数量达到16×16×N个。基于改进YOLO-V2网络设计手势识别模型,模型的输入为人机交互图、提取的手势轮廓与Hu矩,实现交互图像手势识别。测试结果表明,设计方法在室内和室外的手势识别准确率均较高,通过该方法的手势识别结果能够实现人机交互。

一种改进的Hu不变矩算法在存储介质图像识别中的应用

不同于传统的接触和射频两种方式识别存储介质,利用X射线透照目标对象作为检测手段,这种检测方法符合无损检测的特征。首先采用灰度分组(GLG)的图像增强方法和基于单像素提取边缘实现图像预处理,然后利用改进的Hu不变矩得到适合非线性不连续空间的矩不变特征向量,新的不变矩被证明作用于图像的平移、旋转和缩放具有不变性。K-means分类器根据计算不同的形态变换后图像的特征值判断存储介质种类,准确率达到95.83%,证明改进的Hu不变矩算法对被检存储介质种类的分类和识别有较好的鲁棒性和可靠性。实验结果表明,该识别方法可以使涉密的移动存储介质处于安全监控状态下,该技术应用于邮政连续包分机小像素X射线检测设备,可大大减少物联网时代通过邮路途径实施非法媒介传输。

基于改进Hu矩算法的Kinect手势识别

针对Kinect深度信息下静态手势识别鲁棒性差及识别率低的问题,在改进Hu矩算法的基础上提出新的手势识别方法。构造一种新的Hu不变矩,采用尺度归一法消除尺度变化对Hu矩的影响,使静态手势在离散状态下保持手势的比例、平移以及旋转不变性。通过微软新型Kinect传感器和Open NI获取深度图像,对其进行去噪等预处理,基于灰度直方图进行手势分割,提取手指个数特征,并利用改进的Hu矩不变矩算法识别静态手势。实验结果表明,该方法在光照变化、复杂背景等干扰下具有强鲁棒性和高识别率。

水电机组故障诊断专家系统运行状态智能预测及故障识别

对水电设备进行智能预测可以将故障诊断提前至故障发生前,由此可预防不稳定运行对机组设备的损害,延长机组寿命。本文基于ARMA模型、匹配矩阵模型(MM)和Hu不变矩原理,针对水电机组需要重点监控的三类数据:自相关性较强参数、自相关性弱参数、轴心轨迹参数,开发了一套水电机组智能预测和故障识别系统。本系统自2019年实际装备以来预测结果和实际数据拟合良好,将各类潜在故障数据输入系统,系统均可预测并准确判断故障类型。综上,本系统可实现对故障的提前预测和诊断,有力保障水电机组的正常稳定发电。

不同扫描条件下CBCT影像质量及其HU值长期稳定性分析

目的分析研究不同扫描条件下CBCT影像质量及其HU值长期稳定性。方法利用OBI系统对Catphan 504模体在不同临床条件下进行扫描,利用方差分析近一年影像数据的差异性。结果不同条件下CBCT影像密度分辨力均满足Varian验收要求;Full-fan扫描条件下空间分辨力(≥7 lp/cm)优于Half-fan(≥5 lp/cm);空间线性距离及角度显示精度均很好(s<±1%);Air插棒HU值比较均匀,其余密度插棒HU值随扫描条件不同而存在显著性差异(F=660.84,P=0);一年内不同扫描条件HU均值显示较好的一致性。结论不同扫描条件下CBCT影像HU值一致性存在一定差异,但具有较好的长期稳定性,从而为CBCT影像直接用于自适应放疗中剂量计算奠定基础。

抗Hu抗体检测在神经系统副肿瘤综合征诊断中的临床意义

目的探讨抗Hu抗体检测在神经系统副肿瘤综合征诊断中的临床意义。方法采用间接免疫荧光方法和蛋白免疫印迹法对送检至北京协和医院神经病理实验室的1500余例患者的血清和脑脊液进行抗Hu抗体检测,回顾性分析抗Hu抗体阳性患者的临床资料及诊断。神经系统副肿瘤综合征的诊断标准参照Graus等的诊断标准。结果共有27例患者抗体阳性,其中血清抗Hu抗体阳性25例,脑脊液抗Hu抗体阳性8例。临床主要表现为感觉神经元神经病、亚急性小脑变性、Lambert-Eaton综合征和抗利尿激素分泌不当综合征引起的脑病等,其中20例(74.1%)患有肿瘤,包括肺癌17例,胃癌2例,不明性质腹部肿瘤1例。7例患者未发现恶性肿瘤,包括多发性肌炎和系统性红斑狼疮(SLE)合并神经肌肉病各1例。根据Graus等神经系统副肿瘤综合征的诊断标准,27例中22例可确诊神经系统副肿瘤综合征或相关的肿瘤,抗Hu抗体的阳性预测值为81.5%。结论抗Hu抗体阳性对神经系统副肿瘤综合征的诊断具有一定意义;其相关肿瘤以肺癌,特别是小细胞肺癌最常见。其他自身免疫性疾病偶可见抗Hu抗体阳性,需要全面检查和密切随访以排除恶性肿瘤的可能。

肤色检测和Hu矩在安全帽识别中的应用

安全帽在工业生产中的应用非常广泛,为了防止事故发生,确保生产安全,建立对安全帽的自动检测及报警系统变得越来越迫切。主要对安全帽的识别算法进行了研究,采用肤色检测的方法定位到人脸区域,并以此获得脸部以上的区域图像,将Hu矩作为图像的特征向量,分别比较神经网络和支持向量机(SVM)两种分类模型。实验结果表明:SVM对安全帽的识别有很好的效果,将会对监控系统实现智能化提供有力的支持和实际的指导意义。