Assessment of CH_(4) flux and its influencing drivers in the rice-wheat agroecosystem of the Huai River Basin,China

To understand the CH_(4) flux variations and their climatic drivers in the rice-wheat agroecosystem in the Huai River Basin of China,the CH_(4) flux was observed by using open-path eddy covariance at a typical rice-wheat rotation system in Anhui Province,China from November 2019 to October 2021.The variations and their drivers were then analyzed with the Akaike information criterion method.CH_(4) flux showed distinct diurnal variations with single peaks during 9:00-13:00 local time.The highest peak was 2.15μg m^(-2)s^(-1)which occurred at 11:00 in the vegetative growth stage in the rice growing season(RGS).CH_(4) flux also showed significant seasonal variations.The average CH_(4)flux in the vegetative growth stage in the RGS(193.8±74.2 mg m^(-2)d^(-1))was the highest among all growth stages.The annual total CH_(4) flux in the non-rice growing season(3.2 g m^(-2))was relatively small compared to that in the RGS(23.9 g m^(-2)).CH_(4) flux increased significantly with increase in air temperature,soil temperature,and soil water content in both the RGS and the non-RGS,while it decreased significantly with increase in vapor pressure deficit in the RGS.This study provided a comprehensive understanding of the CH_(4) flux and its drivers in the rice-wheat rotation agroecosystem in the Huai River Basin of China.In addition,our findings will be helpful for the validation and adjustment of the CH_(4) models in this region.

Simulation and Analysis of Land System Structure Changes in Huang-Huai-Hai Plain Area

The dynamic changes of land system in Huang - Huai - Hai Plain between 1988 and 2000 were researched in this paper. Spatial dominance econometric model was estabilished on 1 km cell to quantificafionally analyze the driving-force for the dynamic change mechanism of land system, such as natural, social and economic factors. The future dynamic changes of land system in Huang - Huai - Hai Plain on each 1 km cell during 2000 to 2020 were stimulated by combining the dynamic changes of land system on each 1 km cell with different situations. The research indicated that the dynamic changes of land system structure changed mainly from the cultivated areas to building areas and industrial areas, and forest areas increased during this period. Although the revolutions of land system structure were different during 2000 to 2020 with the different referrence standard, ecological protection and economic development, the primary dynamic changes of land system structure were that the increase of building land areas with the decline cuhivaled land areas and the increase of woodlands.

Analyses on cancer incidence and mortality in Huai’an area,China,from 2009 to 2011

Objective： The aim of the study was to investigate the cancer incidence and mortality in Huai＇an area, China, from 2009 to 2011. Methods： The data about cancer incidence and mortality were provided by Huai＇an Cancer Registry, China. Incidence and mortality rates, and standardized rates were calculated by age, gender, areas （urban and rural areas of Huai＇an） and cancer sites. Results： The crude incidence rate for all cancer sites was 205.60/105 and the standardized incidence rate was 166.22/10. Both the crude and standardized rates were higher in urban area than in rural area for both sexes. The inci- dence rates increased in people aged 40 and over, and the peak ages of incidence were between 70-75 in both males and females. The crude mortality rate for all cancer sites was 153.88/105 and the standardized mortality rate was 122.14/105. Both the crude and standardized rates were similar in urban and rural areas for both men and women. The mortality rates were at low level under the age 50 in both sexes, but increased after the age 50, reaching the peak at the ages of 80-85 in both males and females. The top 10 most common cancer sites in rank were esophagus, stomach, lung, liver, colon-rectum, breast, pancreas, cervix uteri, brain and central nervous system, and leukemia, accounting for 87.56% of all cancers. The top 10 most leading causes of cancer death in order were cancers of esophagus, lung, liver, stomach, colon-rectum, pancreas, brain and central nervous system, leukemia, breast and lymphoma, accounting for 90.53% of all cancer deaths. Conclusion： Cancer is one kind of major diseases threatening people＇s health in Huai＇an area, China. Cancer prevention and control should be enhanced, especially for esophageal cancer.

Problems in Landscaping of Residential Areas in Huai'an City and Countermeasures

The present landscaping in residential areas of Huai'an City was introduced in this study, problems emerging from the landscaping were analyzed, and corresponding measures were proposed.

Analysis on the epidemiological characteristics of esophageal cancer in Huai’an area,China from 2009 to 2011

Objective: The aim of our study was to investigate the epidemiological characteristics of esophageal cancer from 2009 to 2011 in Huai'an area, China. Methods: The data about the incidence and mortality of esophageal cancer were provid- ed by Huai'an Cancer Registry, and the epidemiological characteristics of the disease were analyzed. Results: Esophageal cancer was not only the first most common cancer, but also the leading cause of cancer death in Huai'an area. The crude and standardized incidence rates were 62.91/10 5 and 49.92/10 5 , and the crude and standardized mortality rates were 46.75/10 5 and 36.87/10 5 , respectively. The sex ratio (male-female) was 1.69:1 in incidence, and the incidence increased in people aged 40 years and over, reaching the peak at the ages of 70-75 years. The mortality rate was at low level under the age of 50 years, but increased after the age of 50 years, reaching the peak at the age of 75-85 years. Incidence and mortality rates varied regionally with the highest rate found in Chuzhou district (90.76/10 5 and 67.17/10 5 ) and lowest rate observed in Qinghe district (32.41/10 5 and 8.75/10 5 ). Conclusion: Esophageal cancer is the major burden of cancer in Huai'an area, and has marked geographic distribution difference. The key period of age for screening and prevention of the disease is 55-85 years old.

Tillage and straw mulching impacts on grain yield and water use efficiency of spring maize in Northern Huang–Huai–Hai Valley

A two-year field experiment(2012–2013) was conducted to investigate the effects of two tillage methods and five maize straw mulching patterns on the yield, water consumption,and water use efficiency(WUE) of spring maize(Zea mays L.) in the northern Huang–Huai–Hai valley of China. Compared to rotary tillage, subsoil tillage resulted in decreases in water consumption by 6.3–7.8% and increases in maize yield by 644.5–673.9 kg ha-1, soil water content by 2.9–3.0%, and WUE by 12.7–15.2%. Chopped straw mulching led to higher yield,soil water content, and WUE as well as lower water consumption than prostrate whole straw mulching. Mulching with 50% chopped straw had the largest positive effects on maize yield, soil water content, and WUE among the five mulching treatments. Tillage had greater influence on maize yield than straw mulching, whereas straw mulching had greater influence on soil water content, water consumption, and WUE than tillage. These results suggest that 50% chopped straw mulching with subsoil tillage is beneficial in spring maize production aiming at high yield and high WUE in the Huang–Huai–Hai valley.

Effectiveness of Huai Qi Huang Granules on Juvenile Collagen-induced Arthritis and Its Influence on Pyroptosis Pathway in Synovial Tissue

Summary:Huai Qi Huang(HQH)exerts great effects in clinic,such as anti-inflammation,immune-regulation,anti-cancer,and so on.However,the mechanism by which HQH protects juvenile idiopathic arthritis(JIA)is obscure.Thus,we explored deeply the protective mechanisms in juvenile collagen-induced arthritis(CIA)rat model.Pyroptosis is Gasdermin D(GSDMD)-dependent programmed cell death,involved in many diseases,such as sepsis.We investigated whether GSDMD-induced pyroptosis take part in mechanisms of juvenile CIA arthritis.Juvenile Wistar rats(3-4 weeks)were injected intradermally with fully emulsified bovine typeⅡcollagen and complete Freund's adjuvant to establish CIA rat models.Later,the CIA rats received oral administration of HQH(4.16 g/kg)once a day from the day 21 of modeling,with the treatment lasting for 28 days.Varieties of indicators were measured for evaluation of anti-inflammation effect of HQH,including hind paw swelling,arthritis scores,micro CT,and histopathological changes and the level of pro-inflammatory cytokines in the serum,including tumor necrosis factor alpha(TNF-α)and interleukin-18(IL-18).The expression of GSDMD and caspasein the joint synovial tissues was detected.The results demonstrated that the expression of the pyroptotic protein GSDMD and its upstream caspase-1 was significantly increased in the synovial tissues of CIA rats.The treatment of HQH ameliorated the symptoms in CIA rats,reduced levels of pro-inflammatory cytokines and hind paw swelling,down-regulated the expression of GDSMD and caspase-1.GSDMDinduced pyroptosis participated in the pathogenesis of CIA rats.The study supported that HQH can effectively improve joints inflammation of juvenile collagen-induced arthritis rats by inhibiting pyroptosis pathway in the joint synovial tissues.

Sirolimus increases the anti-cancer effect of Huai Er by regulating hypoxia inducible factor-1α-mediated glycolysis in hepatocellular carcinoma

BACKGROUND Glycolysis caused by hypoxia-induced abnormal activation of hypoxia inducible factor-1α(HIF-1α)in the immune microenvironment promotes the progression of hepatocellular carcinoma(HCC),leading to enhanced drug resistance in cancer cells.Therefore,altering the immunosuppressive microenvironment by improving the hypoxic state is a new goal in improving cancer treatment.AIM To analyse the role of HIF-1α,which is closely related to tumour proliferation,invasion,metastasis,and angiogenesis,in the proliferation and invasion of liver cancer,and to explore the HIF-1αpathway-mediated anti-cancer mechanism of sirolimus(SRL)combined with Huai Er.METHODS Previous studies on HCC tissues identified the importance of HIF-1α,glucose transporter 1(GLUT1),and lactate dehydrogenase A(LDHA)expression.In this study,HepG2 and Huh7 cell lines were treated,under hypoxic and normoxic conditions,with a combination of SRL and Huai Er.The effects on proliferation,invasion,cell cycle,and apoptosis were analysed.Proteomics and genomics techniques were used to analyze the HIF-1α-related signalling pathway during SRL combined with Huai Er treatment and its inhibition of the proliferation of HCC cells.RESULTS High levels of HIF-1α,LDHA,and GLUT-1 were found in poorly differentiated HCC,with lower patient survival rates.Hypoxia promoted the proliferation of HepG2 and Huh7 cells and weakened the apoptosis and cell cycle blocking effects of the SRL/Huai Er treatment.This was achieved by activation of HIF-1αand glycolysis in HCC,leading to the upregulation of LDHA,GLUT-1,Akt/mammalian target of rapamycin(mTOR),vascular endothelial growth factor(VEGF),and Forkhead box P3 and downregulation of phosphatase and tensin homolog deleted on chromosome ten(PTEN)and p27.The hypoxia-induced activation of HIF-1αshowed the greatest attenuation in the SRL/Huai Er(S50+H8)group compared to the drug treatments alone(P<0.001).The S50+H8 treatment significantly downregulated the expression of mTOR and HIF-1α,and significantly reduced the expression of VEGF mRNA.Meanwhile,the combined blocking of mTOR and HIF-1αenhanced the downregulation of Akt/mTOR,HIF-1α,LDHA,and GLUT-1 mRNA and resulted in the downregulation of PTEN,p27,and VEGF mRNA(P<0.001).CONCLUSION SRL increases the anti-cancer effect of Huai Er,which reduces the promotion of hypoxia-induced HIF-1αon the Warburg effect by inhibition of the PI3K/Akt/mTOR-HIF-1αand HIF-1α-PTEN signalling pathways in HCC.

Real-time flood forecasting of Huai River with flood diversion and retarding areas

A combination of the rainfall-runoff module of the Xin’anjiang model, the Muskingum routing method, the water stage simulating hydrologic method, the diffusion wave nonlinear water stage method, and the real-time error correction method is applied to the real-time flood forecasting and regulation of the Huai River with flood diversion and retarding areas. The Xin’anjiang model is used to forecast the flood discharge hydrograph of the upstream and tributary. The flood routing of the main channel and flood diversion areas is based on the Muskingum method. The water stage of the downstream boundary condition is calculated with the water stage simulating hydrologic method and the water stages of each cross section are calculated from downstream to upstream with the diffusion wave nonlinear water stage method. The input flood discharge hydrograph from the main channel to the flood diversion area is estimated with the fixed split ratio of the main channel discharge. The flood flow inside the flood retarding area is calculated as a reservoir with the water balance method. The faded-memory forgetting factor least square of error series is used as the real-time error correction method for forecasting discharge and water stage. As an example, the combined models were applied to flood forecasting and regulation of the upper reaches of the Huai River above Lutaizi during the 2007 flood season. The forecast achieves a high accuracy and the results show that the combined models provide a scientific way of flood forecasting and regulation for a complex watershed with flood diversion and retarding areas.

Study on Relationship Between Polymorphisms in FSHβ,ESR,PRLR Genes of A Novel Huai Pig Line II and Its Litter Size

tract By employing PCR-RFLP technique, we detected the polymorphisms in FSHβ, ESR, PRLR genes of a novel Huai pig line II （generation 1 ） and their effects on initial litter size, and analyzed the aggregation effect of multi-gene combined genotype on initial litter size. The results showed that the genotype frequencies of ESR and PRLR genes were in Hardy-Weinberg equilibrium other than the genotype frequency of FSHβ gene. Allele A of FSHβ, ESR and PRLR gene had a positive additive effect on reproductive traits. The favorable genotypes of FSHβ and ESR genes was AA- type, while in PRLR gene was AB-type in the 112 pig individuals detected. With regard to FSHβ and ESR genes, AA-type individuals＇ TNB of were respectively 1.54 （P 〈0.05） and 2.13 （P 〈 0.01 ） higher than the BB-type individuals＇; AA-type individuals＇ AN B of the two genes were respectively 1.55 （ P 〈 0.05） and 1.82 （ P 〈 0.01 ） higher than the BB-type individuals＇. Concerning PRLR gene, AB-type individuals＇ TNB was 0.77 （ P 〉 0.05 ） and 1.59（P〈0.05） higher than that of AA and BB-type individuals respectively, and ANB was 0.65（P〉0.05） and 1.8（ P〈0.01 ） higher than AA and BB-type individuals＇ respectively. Moreover, our results also showed that the AAAABB combination genotype of FSHβ , ESR, PRLR genes bore more than all other combination genotypes, but only 2 individuals were proved to be AAAABB genotype. Consequently, pyramiding FSHβ and ESR genes could to a large extent enhance TNB and NBA of individuals. Therefore, we advise, in next generation that those individuals with AAAA combination genotype of FSHβ and ESR should be selected to increase frequency of allele A, whether PRLR gene is proper for pyramiding with the other genes or not is still to be studied further.

Assessment of dam impacts on river flow regimes and water quality:a case study of the Huai River Basin in P.R.China

The Huai River Basin is a unique area in P.R.China with the highest densities of population and water projects.It is also subject to the most serious water pollution.We proposed a distributional SWAT(Soil and Water Assessment Tool) model coupled with a water quality-quantity balance model to evaluate dam impacts on river flow regimes and water quality in the middle and upper reaches of the Huai River Basin.We calibrated and validated the SWAT model with data from 29 selected cross-sections in four typical years(1971,1981,1991 and 1999) and used scenario analysis to compensate for the unavailability of historical data regarding uninterrupted river flows before dam and floodgate construction,a problem of prediction for ungauged basins.The results indicate that dam and floodgate operations tended to reduce runoff,decrease peak value and shift peaking time.The contribution of water projects to river water quality deterioration in the concerned river system was between 0 to 40%,while pollutant discharge contributed to 60% to 100% of the water pollution.Pollution control should therefore be the key to the water quality rehabilitation in the Huai River Basin.

Estimating daily actual evapotranspiration of a rice–wheat rotation system in typical farmland in the Huai River Basin using a two-step model and two one-step models

The objective of this study is to evaluate the performance of three models for estimating daily evapotranspiration(ET) by employing flux observation data from three years(2007, 2008 and 2009) during the growing seasons of winter wheat and rice crops cultivated in a farmland ecosystem(Shouxian County) located in the Huai River Basin(HRB), China. The first model is a two-step model(PM-Kc);the other two are one-step models(e.g., Rana-Katerji(R-K) and advection-aridity(AA)). The results showed that the energy closure degrees of eddy covariance(EC) data during winter wheat and rice-growing seasons were reasonable in the HRB, with values ranging from 0.84 to 0.91 and R2 of approximately 0.80. Daily ET of winter wheat showed a slow decreasing trend followed by a rapid increase, while that of rice presented a decreasing trend after an increase. After calibrating the crop coefficient(Kc), the PM–Kc model performed better than the model using the Kc recommended by the Food and Agricultural Organization(FAO). The calibrated key parameters of the R-K model and AA model showed better universality. After calibration, the simulation performance of the PM-Kc model was satisfactory. Both the R-K model and AA model underestimated the daily ET of winter wheat and rice. Compared with that of the R-K model, the simulation result of the AA model was better, especially in the simulation of daily ET of rice. Overall, this research highlighted the consistency of the PM-Kc model to estimate the water demand for rice and wheat crops in the HRB and in similar climatic regions in the world.

Analyses on Cancer Incidence and Mortality in Huai’an Area, China, 2010

Objective: The aim of this study was to investigate the cancer incidence and mortality in Huai’an area, China, 2010. Methods: On basis of the criteria of data quality from National Central Cancer Registry (NCCR), data submitted from 8 registries in Huai’an area in 2010 were checked and evaluated. Descriptive analysis included incidence and mortality stratified by areas (urban/rural), sex, age group and cancer sites. Results: The crude incidence rate was 231.17/100,000 (males 276.04/100,000, females 183.87/100,000), age-standardized incidence rate by Chinese standard population (ASIRC) was 179.53/100,000 and by World standard population (ASIRW) was 177.24/ 100,000. The incidence rates increased rapidly in people aged 40 and over, and the peak ages of incidence were at 75 - 79 (males over 85, females at 75 - 79). The cancer mortality rate was 151.26/100,000 (186.06/100,000 in males and 114.58/100,000 in females), age-standardized mortality rate by Chinese standard population (ASMRC) was 116.67/100,000 and by World standard population (ASMRW) was 115.64/100,000. The mortality rates increased rapidly after the age of 50, reaching the peak at the ages over 85 in both males and females. Esophageal, lung, gastric, liver, colorectal, breast, pancreas, lymphoma, brain and central nervous system,?cervical were the most common cancer incident sites. Esophageal, lung, liver, gastric, colorectal, pancreatic, brain and central nervous system, leukemia, breast and lymphoma were the most common cancer sites of death. Conclusions: The data quality of cancer registration is improving. As cancer burden remained high and was different between urban and rural areas, prevention and control should be implemented based on practical situation.

Dominant variation modes associated with Yangtze–Huai River Basin summer heavy rainfall events

The Yangtze–Huai River Basin(YHRB)always suffers from anomalously heavy rainfall during the warm season,and has been well explored as a whole area during the past several decades.In this study,the YHRB is divided into two core regions-the northern YHRB(nYHRB)and southern YHRB(sYHRB)-based on 29-year(1979–2007)June–July–August(JJA)temporally averaged daily rainfall rates and the standard deviation of rainfall.A spectral analysis of JJA daily rainfall data over these 29 years reveals that a 3–7-day synoptic-timescale high-frequency mode is absolutely dominant over the nYHRB,with 10–20-day and 15–40-day modes playing a secondary role.By contrast,3–7-day and 10–20-day modes are both significant over the sYHRB,with 7–14-day,15–40-day,and 20–60-day modes playing secondary roles.Based on a comparison between bandpass-filtered rainfall anomalies and original rainfall series,a total of 42,1,5,and 3 heavy rainfall events(daily rainfall amounts in the top 5%of rainy days)are detected over the nYHRB,corresponding to 3–7-day,7–14-day,10–20-day,and 15–40-day variation disturbances.Meanwhile,a total of 28,8,12,and 6 heavy rainfall events are detected over the sYHRB,corresponding to 3–7-day,7–14-day,10–20-day,and 20–60-day variation disturbances.The results have important implications for understanding the duration of summer heavy rainfall events over both regions.

New Challenges and Opportunities for Flood Control in the Huai River: Addressing a Changing River-Lake Relationship

This paper addresses the change of the river-lake relationship in the Huai River and its causes due to environmental change and human activities. A preliminary analysis is made from three aspects: (1) the natural geographical change particularly captured by the Yellow River, (2) water conservancy project construction, and (3) socioeconomic development in the Huai River Basin. Key problems of changes in this river-lake relationship and the Huai River flood control are tackled, involving flood control and disaster alleviation ability of the Basin, engineering and non-engineering measurements applied to flood control and disaster mitigation, and water governance for adaptive management. Research shows that the Huai River is a rather complex one due to its complex geography with a hybrid wet and dry climate zoon, and higher population density. With the alternation of the river-lake relationship and socioeconomic development in the region, new problems keep arising, imposing new requirements on its sustainable water management. Thus, understanding the Huai River is a long and gradually improving process. Its future planning should keep absorbing new achievements of science and technology development, employing new technologies and methods, and gradually deepening our understanding of its fundamental principles. Water governance and adaptive water management will be new challenges and opportunities for the Basin in its river system change and flood control.

Climate Change and its Impact on Water Resources in the Huai River Basin

Rainfall and air temperature data from six meteorological stations above the Bengbu Sluice and hydrological and water resources evaluation data from the Bengbu Hydrological Station in the Huai River Basin from 1961 to 2008 are used to analyze the impact of changes in climatic factors on the amount of water resources in the Basin. There was a general trend of rise in its average annual air temperature, with the highest increase of 0.289℃/10a recorded at Bengbu in Anhui Province. Rising rainfall was mainly observed in the western part of the study area, while rainfall actually declined in the eastern part, i.e. the middle reaches of the Huai River. The Average rainfall in the study area was in a vaguely declining trend. In other words, the rainfall in the Basin is still much affected by natural fluctuations. On the whole, there was a trend of gradual decrease in the quantity of the Basin＇s water resources for the period under study. Water resources quantity is found to fall with decreasing rainfall and rising air temperature. Regression analysis is used to establish a mathematical model between water resources quantity and climatic factors （i.e. air temperature and rainfall） in order to explore the impact of climate change on water resources in the Basin. Moreover, various scenarios are set to quantitatively analyze the response of water resources to climate change. Sensitivity analysis shows that changes in rainfall have a much bigger impact on its water resources quantity than changes in its air temperature.

Huai Qi Huang-induced Apoptosis via Down-regulating PRKCH and Inhibiting RAF/MEK/ERK Pathway in Ph+ Leukemia Cells

Imatinib mesylate(IM)is the first-line treatment for Philadelphia(Ph)chromosomal positive leukemia by inhibiting phosphorylation of substrates via binding to the ABL kinase domain.Because of the drug resistance,side effects and the high cost of IM,it is necessary to find anti-cancer drugs with relatively low toxicity and cost,and enhanced efficacy,such as traditional Chinese medicines(TCMs).As one of TCMs,Huai Qi Huang(HQH)was chosen to treat BV173 and K562 cells.Various concentrations of HQH were added to cells for 24-72 h.Co-treatment of HQH and trametinib,an MEK inhibitor,was used to verify the synergistic effects on cell viability and apoptosis.Knockdown and overexpression of mitogen-activated protein kinase kinase 4(MEK4)were implemented to demonstrate the role of MEK in cell apoptosis.Cell viability and apoptosis were measured by cell counting kit-8 assay(CCK8)and flow cytometry,respectively.Western blotting and real-time quantitative PCR(RT-qPCR)were used to assess protein and mRNA expression levels,respectively.The results showed that HQH inhibited survival and promoted apoptosis of BV173 and K562 cells in a dose-dependent manner,accompanied with down-regulation of PRKCH mRNA as well as CRAF,MEK4,phospho-ERK(pERK)and BCL2 proteins,and up-regulation of cleaved caspase3 protein.Co-treatment of HQH and trametinib had.a synergistic effect on inhibiting survival and promoting apoptosis.MEK4 knockdown increased apoptosis,and had a synergistic effeet with HQH.In contrast,MEK4 overexpression decreased.apoptosis,and had the opposite efect with HQH.Collectively,the results of this study may identify a therapeutic mechanism of HQH on promoting apoptosis,and provide a potential option for treatment of Pht leukemia.

Production Structure of Agriculture in Huai'an City

According to the data in 1990-2009 Huai'an Statistical Yearbook and Jiangsu Statistical Yearbook,production structure of agriculture in Huai'an City is analyzed.Result shows that economic efficiency of planting industry is significantly lower than that of other industry types of agriculture;and the production structure of agriculture in Huai'an City is unbalanced and needs further optimization.The reasons for the imbalance in industrial structure are various,such as the relatively low quality of agricultural employees,the backward marketing of planting,and the lack of standardization management.Finally,corresponding suggestions are put forward,including developing education,conducting vocational training,improving the human capital stock of Huai'an City,cultivating the leading enterprises for agricultural product processing,and realizing the scale agglomeration effect of agricultural production in planting industry.

CHINESE HUAI TANG NINE ACUPUNCTURE NEEDLES: RESEARCH ON THE NEW NINE ACUPUNCTURE NEEDLES THERAPY AND THE NEEDLING INSTRUMENTS (CONTINUED)

PART TWOThe Research on Chinese Huai TangNine Acupuncture Needles TherapyI The theoretical basis of ChineseHuai Tang Nine Needles Therapy.1) The kernel of the theoretical basis ofChinese Huai Tang Nine Needles Therapy.Chinese Huai Tang Nine Needles Therapyis to give full play to the treatment action of thespecifity and entirety of each needle. The speci-fity means that each needle has its own

Kinetics of medium-temperature α-amylase hydrolyzed <i>Huai yam</i>powder

In order to learn the enzymatic characteristics of Huai yam powder with medium-temperatureα-amylase, effects of substrate concentration,enzyme concentration, pH and temperature wereinvestigated. The Michealis-Menten equationwas used to fit the kinetics of the hydrolysis reaction. Experimental results indicate that maximum rate (Vm) is 3.1588 mg/mL·min under the condition of 70&#176;C, pH 7.0 and 0.0200 mg/mL of enzyme concentration. The Michealis constant (Km) is 6.6641 mg/mL. The kinetic model, including the factors such as substrate concentration, enzyme concentration and temperature, was established for the hydrolysis reaction under the temperature range from 40&#176;C - 70&#176;C.