Pien Tze Huang(PZH),a class-1 nationally protected traditional Chinese medicine(TCM),has been used to treat liver diseases such as hepatitis;however,the effect of PZH on the progression of sepsis is unknown.Here,we re...Pien Tze Huang(PZH),a class-1 nationally protected traditional Chinese medicine(TCM),has been used to treat liver diseases such as hepatitis;however,the effect of PZH on the progression of sepsis is unknown.Here,we reported that PZH attenuated lipopolysaccharide(LPS)-induced sepsis in mice and reduced LPS-induced production of proinflammatory cytokines in macrophages by inhibiting the activation of mitogen-activated protein kinase(MAPK)and nuclear factor-kappa B(NF-κB)signalling.Mechanistically,PZH stimulated signal transducer and activator of transcription 3(STAT3)phosphorylation to induce the expression of A20,which could inhibit the activation of NF-κB and MAPK signalling.Knockdown of the bile acid(BA)receptor G protein-coupled bile acid receptor 1(TGR5)in macrophages abolished the effects of PZH on STAT3 phosphorylation and A20 induction,as well as the LPS-induced inflammatory response,suggesting that BAs in PZH may mediate its anti-inflammatory effects by activating TGR5.Consistently,deprivation of BAs in PZH by cholestyramine resin reduced the effects of PZH on the expression of phosphorylated-STAT3 and A20,the activation of NF-κB and MAPK signalling,and the production of proinflammatory cytokines,whereas the addition of BAs to cholestyramine resin-treated PZH partially restored the inhibitory effects on the production of proinflammatory cytokines.Overall,our study identifies BAs as the effective components in PZH that activate TGR5-STAT3-A20 signalling to ameliorate LPS-induced sepsis.展开更多
Huang Di Nei Jing(《黄帝内经》The Yellow Emperor’s Inner Classic) has been the source text of Chinese medicine knowledge and innovation for over two thousand years. Despite this key relevance, many of its ideas and p...Huang Di Nei Jing(《黄帝内经》The Yellow Emperor’s Inner Classic) has been the source text of Chinese medicine knowledge and innovation for over two thousand years. Despite this key relevance, many of its ideas and practices have proven difficult to understand and implement fully into clinical practice. Cultural and language differences can be compounded with these challenges but may also present new opportunities for advancement and insight when studied by researchers outside of the originating culture. This article introduces the method of Classical-Text Archaeology and delves into the author’s two-decade journey of researching this text, with a discussion on cultural differences and issues of medical scholarship.展开更多
BACKGROUND Traditional Chinese medicine has used the drug Pien Tze Huang(PTH),a classic prescription,to treat autoimmune hepatitis(AIH).However,the precise mode of action is still unknown.AIM To investigate the mechan...BACKGROUND Traditional Chinese medicine has used the drug Pien Tze Huang(PTH),a classic prescription,to treat autoimmune hepatitis(AIH).However,the precise mode of action is still unknown.AIM To investigate the mechanism of PTH in an AIH mouse model by determining the changes in gut microbiota structure and memory regulatory T(mTreg)cells functional levels.METHODS Following induction of the AIH mouse model induced by Concanavalin A(Con A),prophylactic administration of PTH was given for 10 d.The levels of mTreg cells were measured by flow cytometry,and intestinal microbiota was analyzed by 16S rRNA analysis,while western blotting was used to identify activation of the toll-like receptor(TLR)2,TLR4/nuclear factor-κB(NF-κB),and CXCL16/CXCR6 signaling pathways.RESULTS In the liver of mice with AIH,PTH relieved the pathological damage and reduced the numbers of T helper type 17 cells and interferon-γ,tumor necrosis factor-alpha,interleukin(IL)-1β,IL-2,IL-6,and IL-21 expression.Simultaneously,PTH stimulated the abundance of helpful bacteria,promoted activation of the TLR2 signal,which may enhance Treg/mTreg cells quantity to produce IL-10,and suppressed activation of the TLR4/NF-κB and CXCL16/CXCR6 signaling pathways.CONCLUSION PTH regulates intestinal microbiota balance and restores mTreg cells to alleviate experimental AIH,which is closely related to the TLR/CXCL16/CXCR6/NF-κB signaling pathway.展开更多
From September 6 to 7,the 6th Silk Road(Dunhuang)International Cultural Expo was successful held in Dunhuang.The theme of this year's Dunhuang Expo was"Connecting with the World:Cultural Exchanges and Mutual ...From September 6 to 7,the 6th Silk Road(Dunhuang)International Cultural Expo was successful held in Dunhuang.The theme of this year's Dunhuang Expo was"Connecting with the World:Cultural Exchanges and Mutual Learning".It focused on facilitating cooperation under the Belt and Road Initiative(BRI),carrying forward Dunhuang culture.展开更多
On October 27,Organized by the Dunhuang Academy and China Cultural Center in Auckland,the"The Story of China-Dunhuang in the World"salon and"Art of Dunhuang Grottoes"lecture was successfully held a...On October 27,Organized by the Dunhuang Academy and China Cultural Center in Auckland,the"The Story of China-Dunhuang in the World"salon and"Art of Dunhuang Grottoes"lecture was successfully held at the Auckland Art Gallery.Zhao Shengliang,Chairman of the Academic Committee of the Dunhuang Academy,was the key speaker.展开更多
Skilled talents are the main support for the strategic development of science and technology in China.The quality of talents is an important guarantee for the transformation and upgrading of national industries.By ana...Skilled talents are the main support for the strategic development of science and technology in China.The quality of talents is an important guarantee for the transformation and upgrading of national industries.By analyzing and studying Huang Yanpei’s thought on vocational education,this paper focuses on the cultivation of students’craftsmanship in vocational schools.First,we analyze the results of previous research and then elaborate on the connotations of Huang Yanpei’s education thought and craftsmanship spirit.Lastly,we propose five suggestions for the cultivation of craftsmanship.Our research work carries certain reference value to the study of craftsmanship cultivation in Huang Yanpei’s education thought.展开更多
Scholars have previously attributed the theoretical origin of“the heart is the monarch/ruler”to the monarchical view of politics.However,according to the latest unearthed documents and relevant documents handed down...Scholars have previously attributed the theoretical origin of“the heart is the monarch/ruler”to the monarchical view of politics.However,according to the latest unearthed documents and relevant documents handed down from ancient times,it can be found that the theory of“the heart is the monarch”might derive from the importance of attachment to the“Zhong”ideology in traditional culture,which promoted the concept that the heart as the center of body,should be the ruler and was later evolved as the“monarch”.Similar cognitive views were all mentioned in Confucianism,Taoism,eclectics scholars and other pre-Qin literature,but only in the system of traditional Chinese medicine,they played a significant role in building the heart-centered theory of Zang-Fu.Traditional Chinese medicine is a discipline developed mainly by clinical practice with the fundamental purpose of curing diseases and saving people.Although the philosophy of traditional Chinese medicine(TCM)roots in the pre-Qin Dynasty,the development is unique to the other philosophies.With rich cultural connotations and philosophical thoughts,TCM is certainly the key to the treasures of Chinese civilization and deserves more attention and exploration.展开更多
[Objective] The research aimed to screen out optimum RAPD reaction system on genomic DNA of Agrocybe chaxingu Huang.[Method] The single factor experiment was adopted to select the required Mg2+ concentration, template...[Objective] The research aimed to screen out optimum RAPD reaction system on genomic DNA of Agrocybe chaxingu Huang.[Method] The single factor experiment was adopted to select the required Mg2+ concentration, template DNA concentration,primer concentration,dNTPs concentration,Taq enzyme concentration and anneal temperature initially.[Result] The optimum reaction system for RAPD amplification of Agrocybe chaxingu Huang was listed as follows:2.5 μl Buffer, 2 mmol/L Mg2+, 75 ng DNA, 0.5 μmol/L primer, 150 μmol/L dNTPs and 2.0 Taq enzyme.The reaction process was also listed as follows: denaturation for 5 min at 92 ℃,35 cycles(1 min at 92 ℃, 1 min for 35.5 ℃ and elongation for 2 min at 72 ℃),10 min at 72 ℃.[Conclusion] The research provided reference for conducting RAPD analysis of and studying genetic relationship and genetic diversity of Agrocybe chaxingu Huang.展开更多
针对Hilbert-Huang变换方法中由于信号经验模态分解(empirical mode decomposition,简称EMD)过程中所存在的端点效应问题,分析了现有数据延拓方式的利弊,并在基于斜率(slope based method,简称SBM)方法以及改进方法(improved slope base...针对Hilbert-Huang变换方法中由于信号经验模态分解(empirical mode decomposition,简称EMD)过程中所存在的端点效应问题,分析了现有数据延拓方式的利弊,并在基于斜率(slope based method,简称SBM)方法以及改进方法(improved slope based method,简称ISBM)的基础上提出了一种全新的基于斜率再优化(reoptimization slope based method,简称RO-SBM)方法用于信号序列的极值点延拓,然后对延拓后的数据进行EMD分解,得到相应的本征模函数(intrinsic mode function,简称IMF)分量。数值仿真结果表明,采用基于RO-SBM方法进行数据延拓,相比镜像延拓以及ISBM方法,可以更有效地抑制EMD中的端点效应问题,提升HHT方法的信号分析性能。通过基于RO-SBM方法进行数据延拓的HHT方法准确分离出了某转子系统的局部碰摩径向振动信号中所包含的故障特征分量,并将此方法成功应用于旋转机械故障诊断领域。展开更多
基金supported by research funds from Zhangzhou Pien Tze Huang Pharmaceutical Co.Ltd(Grant Nos.:437b8f31,d6092dae,YHT-19064 to Chundong Yu)the National Natural Science Foundation of China(Grant Nos.:81970485,82173086 to Chundong Yu)the Natural Science Foundation of Fujian Province(Grant No.:2023J01249 to Shicong Wang).
文摘Pien Tze Huang(PZH),a class-1 nationally protected traditional Chinese medicine(TCM),has been used to treat liver diseases such as hepatitis;however,the effect of PZH on the progression of sepsis is unknown.Here,we reported that PZH attenuated lipopolysaccharide(LPS)-induced sepsis in mice and reduced LPS-induced production of proinflammatory cytokines in macrophages by inhibiting the activation of mitogen-activated protein kinase(MAPK)and nuclear factor-kappa B(NF-κB)signalling.Mechanistically,PZH stimulated signal transducer and activator of transcription 3(STAT3)phosphorylation to induce the expression of A20,which could inhibit the activation of NF-κB and MAPK signalling.Knockdown of the bile acid(BA)receptor G protein-coupled bile acid receptor 1(TGR5)in macrophages abolished the effects of PZH on STAT3 phosphorylation and A20 induction,as well as the LPS-induced inflammatory response,suggesting that BAs in PZH may mediate its anti-inflammatory effects by activating TGR5.Consistently,deprivation of BAs in PZH by cholestyramine resin reduced the effects of PZH on the expression of phosphorylated-STAT3 and A20,the activation of NF-κB and MAPK signalling,and the production of proinflammatory cytokines,whereas the addition of BAs to cholestyramine resin-treated PZH partially restored the inhibitory effects on the production of proinflammatory cytokines.Overall,our study identifies BAs as the effective components in PZH that activate TGR5-STAT3-A20 signalling to ameliorate LPS-induced sepsis.
文摘Huang Di Nei Jing(《黄帝内经》The Yellow Emperor’s Inner Classic) has been the source text of Chinese medicine knowledge and innovation for over two thousand years. Despite this key relevance, many of its ideas and practices have proven difficult to understand and implement fully into clinical practice. Cultural and language differences can be compounded with these challenges but may also present new opportunities for advancement and insight when studied by researchers outside of the originating culture. This article introduces the method of Classical-Text Archaeology and delves into the author’s two-decade journey of researching this text, with a discussion on cultural differences and issues of medical scholarship.
基金Supported by the National Natural Science Foundation of China,No.81603402,82060798,81860791the Special Fund Project for Graduate Innovation of Jiangxi University of Chinese Medicine,No.JZYC22S77+3 种基金a Special Fund Project for Graduate Innovation of Jiangxi Province,No.YC2022-s840,YC2022-B188Jiangxi University of Chinese Medicine Science and Technology Innovation Team Development Program,No.CXTD22008the Young and Middle-aged Backbone Talent Project of Jiangxi Administration of Traditional Chinese Medicine,No.[2020]05Young Qhuang Scholars support Project of National Administration of Traditional Chinese Medicine,No.[2022]256.
文摘BACKGROUND Traditional Chinese medicine has used the drug Pien Tze Huang(PTH),a classic prescription,to treat autoimmune hepatitis(AIH).However,the precise mode of action is still unknown.AIM To investigate the mechanism of PTH in an AIH mouse model by determining the changes in gut microbiota structure and memory regulatory T(mTreg)cells functional levels.METHODS Following induction of the AIH mouse model induced by Concanavalin A(Con A),prophylactic administration of PTH was given for 10 d.The levels of mTreg cells were measured by flow cytometry,and intestinal microbiota was analyzed by 16S rRNA analysis,while western blotting was used to identify activation of the toll-like receptor(TLR)2,TLR4/nuclear factor-κB(NF-κB),and CXCL16/CXCR6 signaling pathways.RESULTS In the liver of mice with AIH,PTH relieved the pathological damage and reduced the numbers of T helper type 17 cells and interferon-γ,tumor necrosis factor-alpha,interleukin(IL)-1β,IL-2,IL-6,and IL-21 expression.Simultaneously,PTH stimulated the abundance of helpful bacteria,promoted activation of the TLR2 signal,which may enhance Treg/mTreg cells quantity to produce IL-10,and suppressed activation of the TLR4/NF-κB and CXCL16/CXCR6 signaling pathways.CONCLUSION PTH regulates intestinal microbiota balance and restores mTreg cells to alleviate experimental AIH,which is closely related to the TLR/CXCL16/CXCR6/NF-κB signaling pathway.
文摘From September 6 to 7,the 6th Silk Road(Dunhuang)International Cultural Expo was successful held in Dunhuang.The theme of this year's Dunhuang Expo was"Connecting with the World:Cultural Exchanges and Mutual Learning".It focused on facilitating cooperation under the Belt and Road Initiative(BRI),carrying forward Dunhuang culture.
文摘On October 27,Organized by the Dunhuang Academy and China Cultural Center in Auckland,the"The Story of China-Dunhuang in the World"salon and"Art of Dunhuang Grottoes"lecture was successfully held at the Auckland Art Gallery.Zhao Shengliang,Chairman of the Academic Committee of the Dunhuang Academy,was the key speaker.
基金The Project of China Vocational Education Association(Project Number:ZJS2022YB024)The Project of Innovation and Development Center of Ideological and Political Work(Beijing Polytechnic),Ministry of Education(Project Number:2022X305-SXZC).
文摘Skilled talents are the main support for the strategic development of science and technology in China.The quality of talents is an important guarantee for the transformation and upgrading of national industries.By analyzing and studying Huang Yanpei’s thought on vocational education,this paper focuses on the cultivation of students’craftsmanship in vocational schools.First,we analyze the results of previous research and then elaborate on the connotations of Huang Yanpei’s education thought and craftsmanship spirit.Lastly,we propose five suggestions for the cultivation of craftsmanship.Our research work carries certain reference value to the study of craftsmanship cultivation in Huang Yanpei’s education thought.
文摘Scholars have previously attributed the theoretical origin of“the heart is the monarch/ruler”to the monarchical view of politics.However,according to the latest unearthed documents and relevant documents handed down from ancient times,it can be found that the theory of“the heart is the monarch”might derive from the importance of attachment to the“Zhong”ideology in traditional culture,which promoted the concept that the heart as the center of body,should be the ruler and was later evolved as the“monarch”.Similar cognitive views were all mentioned in Confucianism,Taoism,eclectics scholars and other pre-Qin literature,but only in the system of traditional Chinese medicine,they played a significant role in building the heart-centered theory of Zang-Fu.Traditional Chinese medicine is a discipline developed mainly by clinical practice with the fundamental purpose of curing diseases and saving people.Although the philosophy of traditional Chinese medicine(TCM)roots in the pre-Qin Dynasty,the development is unique to the other philosophies.With rich cultural connotations and philosophical thoughts,TCM is certainly the key to the treasures of Chinese civilization and deserves more attention and exploration.
文摘[Objective] The research aimed to screen out optimum RAPD reaction system on genomic DNA of Agrocybe chaxingu Huang.[Method] The single factor experiment was adopted to select the required Mg2+ concentration, template DNA concentration,primer concentration,dNTPs concentration,Taq enzyme concentration and anneal temperature initially.[Result] The optimum reaction system for RAPD amplification of Agrocybe chaxingu Huang was listed as follows:2.5 μl Buffer, 2 mmol/L Mg2+, 75 ng DNA, 0.5 μmol/L primer, 150 μmol/L dNTPs and 2.0 Taq enzyme.The reaction process was also listed as follows: denaturation for 5 min at 92 ℃,35 cycles(1 min at 92 ℃, 1 min for 35.5 ℃ and elongation for 2 min at 72 ℃),10 min at 72 ℃.[Conclusion] The research provided reference for conducting RAPD analysis of and studying genetic relationship and genetic diversity of Agrocybe chaxingu Huang.
文摘针对Hilbert-Huang变换方法中由于信号经验模态分解(empirical mode decomposition,简称EMD)过程中所存在的端点效应问题,分析了现有数据延拓方式的利弊,并在基于斜率(slope based method,简称SBM)方法以及改进方法(improved slope based method,简称ISBM)的基础上提出了一种全新的基于斜率再优化(reoptimization slope based method,简称RO-SBM)方法用于信号序列的极值点延拓,然后对延拓后的数据进行EMD分解,得到相应的本征模函数(intrinsic mode function,简称IMF)分量。数值仿真结果表明,采用基于RO-SBM方法进行数据延拓,相比镜像延拓以及ISBM方法,可以更有效地抑制EMD中的端点效应问题,提升HHT方法的信号分析性能。通过基于RO-SBM方法进行数据延拓的HHT方法准确分离出了某转子系统的局部碰摩径向振动信号中所包含的故障特征分量,并将此方法成功应用于旋转机械故障诊断领域。
