为了建立高效液相色谱(high-performance liquid chromatography,HPLC)指纹图谱结合一测多评法(quantitation analysis of multi-components by single marker,QAMS)评价傣药方剂“黄氏蓝瘟康”(Dai medicine prescription Huang’s Lan...为了建立高效液相色谱(high-performance liquid chromatography,HPLC)指纹图谱结合一测多评法(quantitation analysis of multi-components by single marker,QAMS)评价傣药方剂“黄氏蓝瘟康”(Dai medicine prescription Huang’s Lanwenkang,DMPHL)的质量,试验采用HPLC对11批DMPHL复方的成分进行分析,将所得色谱信息导入“中药色谱指纹图谱相似度评价系统”2012版进行分析,结合混合对照品色谱信息对共有峰进行指认,建立DMPHL的HPLC指纹图谱;以大黄酚为内参物,建立可同时测定绿原酸、盐酸巴马汀、盐酸小檗碱、大黄酸、对甲氧基肉桂酸乙酯、大黄素及大黄酚含量的一测多评法,同时用外标法(external standard method,ESM)验证一测多评法测定结果的准确性。结果表明:11批DMPHL的HPLC指纹图谱共有峰为24个,不同批次DMPHL指纹图谱的相似度均在0.900以上;以大黄酚为内参物计算出绿原酸、盐酸巴马汀、盐酸小檗碱、大黄酸、对甲氧基肉桂酸乙酯、大黄素的相对校正因子分别为2.0170,0.7885,0.8725,7.9264,2.7131,0.6992;一测多评法与外标法测定结果的RSD值均小于3.00%,两种方法的测定结果无明显差异。表明试验所建立的HPLC指纹图谱结合一测多评法适用于DMPHL的质量评价。展开更多
The prescription“Huang's SiYu Pill”(HSYP)was founded by professor Huang Haibo,a famous traditional Chinese medicine in Inner Mongolia,based on long-term clinical experience.In this study we observed the effects ...The prescription“Huang's SiYu Pill”(HSYP)was founded by professor Huang Haibo,a famous traditional Chinese medicine in Inner Mongolia,based on long-term clinical experience.In this study we observed the effects of HYSP on sperm quality,hormone level and the expression level of reproductive related genes and proteins in testicular stromal cells of rats with kidney-yin deficiency to explore the therapeutic mechanism of HSYP in the treatment of infertility with kidney-yin deficiency,Liuwei Dihuang Pill group(LDP)and Tamoxifen Citrate Tablets combined with Pancreatic Kallidinogenase Enteric Coated Tablets were used to be the positive control drug.After 25 days of medicine administration,the animals were anesthetized and killed,the serum and testicular tissues were retained for further experiments.The ELISA results showed that HSYP had a better effect on increasing serum FSH level than the conventional Chinese medicine or western medicine,while there was no remarkable difference in the regulation of serum LH in each administration group.The results of RT-qPCR showed that the transcription levels of Cyp11a1,Hsd3b1,Lhcgr and Star genes in the medium and high dose groups of HSYP were significantly upregulated compared with the model group of kidney-yin deficiency,while the expression of Nr5a1 gene was not significantly affected.The results of HE and immunohistochemistry showed that HSYP significantly improved the development level of testicular stromal cells and promoted the expression levels of CYP11A1 and HSD11B1 in testicular stromal cells,compared with the model group of kidney-yin deficiency.Together,our results showed that Huang's SiYu Pills can significantly improve the development and function of Leydig cells in SD rats with kidney-yin deficiency model,which provided a good research basis for deep research on relevant pharmacological mechanisms of the medicine.展开更多
There have been selected 83 samples of terracotta warriors and horses of Emperor Qin Shi Huang's Mausoleum, 20 samples of clays taken from around Qin's Mausoleum and 2 samples of Yaozhou porcelain bodies. All ...There have been selected 83 samples of terracotta warriors and horses of Emperor Qin Shi Huang's Mausoleum, 20 samples of clays taken from around Qin's Mausoleum and 2 samples of Yaozhou porcelain bodies. All these samples have been measured by instrument neutron activation analysis (INAA) and as many as 32 kinds of element contents of each sample are measured. The following conclusion has been reached when fuzzy cluster analysis is conducted to element contents of all these samples: (i) The samples are roughly classified into five categories: namely, samples from pits No. 1 and No. 2; samples from pit No. 3; loam layers; the mixture of loam and loess; and Yaozhou porcelain bodies. (ii) The terracottawarriors and horses in pits No. 1, No. 2 and No. 3 are relatively independent from one another. The clays from which they were made are not entirely identical.We have found that samples in pit No. 3 are very closely related and their claysources are comparatively concentrated. Samples in pits No. 1 and No. 2 are less related and their clay sources are comparatively scattered. (iii) The clays from which the terracotta warriors and horses were made are closely related to theloam layer near Qin's Mausoleum, particularly to the loam layer of Zaoyuan village and Gaoxing village, but they are not so related to loess layers there, nor to the loam layers of Anhoubao, even less related to Yaozhou porcelain bodies. Arational deduction thus drawn is that the raw material of clays from which the terracotta warriors and horses were made might probably be taken from loam layers around Zaoyuan and Gaoxing, or loam layers near Qin's Mausoleum whose properties are identical with those of loam layers of Zaoyuan and Gaoxing, rather than loess layers around the above places. Since the raw material of the terracotta warriors and horses was taken from loam near Qin's Mausoleum, it could be deductedthat the kiln sites might be located in around Qin's Mausoleum.展开更多
文摘为了建立高效液相色谱(high-performance liquid chromatography,HPLC)指纹图谱结合一测多评法(quantitation analysis of multi-components by single marker,QAMS)评价傣药方剂“黄氏蓝瘟康”(Dai medicine prescription Huang’s Lanwenkang,DMPHL)的质量,试验采用HPLC对11批DMPHL复方的成分进行分析,将所得色谱信息导入“中药色谱指纹图谱相似度评价系统”2012版进行分析,结合混合对照品色谱信息对共有峰进行指认,建立DMPHL的HPLC指纹图谱;以大黄酚为内参物,建立可同时测定绿原酸、盐酸巴马汀、盐酸小檗碱、大黄酸、对甲氧基肉桂酸乙酯、大黄素及大黄酚含量的一测多评法,同时用外标法(external standard method,ESM)验证一测多评法测定结果的准确性。结果表明:11批DMPHL的HPLC指纹图谱共有峰为24个,不同批次DMPHL指纹图谱的相似度均在0.900以上;以大黄酚为内参物计算出绿原酸、盐酸巴马汀、盐酸小檗碱、大黄酸、对甲氧基肉桂酸乙酯、大黄素的相对校正因子分别为2.0170,0.7885,0.8725,7.9264,2.7131,0.6992;一测多评法与外标法测定结果的RSD值均小于3.00%,两种方法的测定结果无明显差异。表明试验所建立的HPLC指纹图谱结合一测多评法适用于DMPHL的质量评价。
基金The present study was supported by the National TCM Innovation Key Talents Training Program(NO.2019128)the National Famous Old TCM Experts Inheritance Studio Construction Project(No.2014252)the Hohhot Applied Technology Research and Development Project(No.2018-she-1-1).
文摘The prescription“Huang's SiYu Pill”(HSYP)was founded by professor Huang Haibo,a famous traditional Chinese medicine in Inner Mongolia,based on long-term clinical experience.In this study we observed the effects of HYSP on sperm quality,hormone level and the expression level of reproductive related genes and proteins in testicular stromal cells of rats with kidney-yin deficiency to explore the therapeutic mechanism of HSYP in the treatment of infertility with kidney-yin deficiency,Liuwei Dihuang Pill group(LDP)and Tamoxifen Citrate Tablets combined with Pancreatic Kallidinogenase Enteric Coated Tablets were used to be the positive control drug.After 25 days of medicine administration,the animals were anesthetized and killed,the serum and testicular tissues were retained for further experiments.The ELISA results showed that HSYP had a better effect on increasing serum FSH level than the conventional Chinese medicine or western medicine,while there was no remarkable difference in the regulation of serum LH in each administration group.The results of RT-qPCR showed that the transcription levels of Cyp11a1,Hsd3b1,Lhcgr and Star genes in the medium and high dose groups of HSYP were significantly upregulated compared with the model group of kidney-yin deficiency,while the expression of Nr5a1 gene was not significantly affected.The results of HE and immunohistochemistry showed that HSYP significantly improved the development level of testicular stromal cells and promoted the expression levels of CYP11A1 and HSD11B1 in testicular stromal cells,compared with the model group of kidney-yin deficiency.Together,our results showed that Huang's SiYu Pills can significantly improve the development and function of Leydig cells in SD rats with kidney-yin deficiency model,which provided a good research basis for deep research on relevant pharmacological mechanisms of the medicine.
文摘There have been selected 83 samples of terracotta warriors and horses of Emperor Qin Shi Huang's Mausoleum, 20 samples of clays taken from around Qin's Mausoleum and 2 samples of Yaozhou porcelain bodies. All these samples have been measured by instrument neutron activation analysis (INAA) and as many as 32 kinds of element contents of each sample are measured. The following conclusion has been reached when fuzzy cluster analysis is conducted to element contents of all these samples: (i) The samples are roughly classified into five categories: namely, samples from pits No. 1 and No. 2; samples from pit No. 3; loam layers; the mixture of loam and loess; and Yaozhou porcelain bodies. (ii) The terracottawarriors and horses in pits No. 1, No. 2 and No. 3 are relatively independent from one another. The clays from which they were made are not entirely identical.We have found that samples in pit No. 3 are very closely related and their claysources are comparatively concentrated. Samples in pits No. 1 and No. 2 are less related and their clay sources are comparatively scattered. (iii) The clays from which the terracotta warriors and horses were made are closely related to theloam layer near Qin's Mausoleum, particularly to the loam layer of Zaoyuan village and Gaoxing village, but they are not so related to loess layers there, nor to the loam layers of Anhoubao, even less related to Yaozhou porcelain bodies. Arational deduction thus drawn is that the raw material of clays from which the terracotta warriors and horses were made might probably be taken from loam layers around Zaoyuan and Gaoxing, or loam layers near Qin's Mausoleum whose properties are identical with those of loam layers of Zaoyuan and Gaoxing, rather than loess layers around the above places. Since the raw material of the terracotta warriors and horses was taken from loam near Qin's Mausoleum, it could be deductedthat the kiln sites might be located in around Qin's Mausoleum.
