Study on Effect of Iron in Anther Media of Early Japonica Rice in Cold Region

In this study, through vitro culturing anthers of 7 F1 progenies of early Japonica rice in cold region on medium with different Fe2+ contents, it was found that Fe2+ content generated greater impacts on the induction rate and green plantlet differentiation. The result demonstrated that if Fe2+ increased from 32 to 40 mg/kg, the induction rate of early Japonica rice anther culture in N6 culture media was more then 1.4 times higher than that in N6 culture media containing 5.6 mg/kg Fe2+. In this concentration range, the induction rate increased with the increase of Fe2+ content, while if the concentration was over this concentration range, the induction rate decreased with the increase of Fe2+, showing single peak distribution. When the Fe2+ was 40 mg/kg in differentiation medium, the differentiation rate decreased dramatically. The green plantlet differentiations of callus which were induced on culture media containing 32-40 mg/kg Fe2+ were different, when they were cultured on MS culture media, and 85.7% materials could increase green plantlet productivity to about 7.8%. Therefore, increasing Fe2+in induction media properly could increase anther culture efficiency of early Japonica rice in cold region.

Characteristics and Transformation of Heavy Metal Pollution in Soil and Rice of Yangtze River Delta Region

[Objective]Characteristics of heavy metal pollution in soil and rice of Yangtze River Delta Region were studied.[Method]Heavy metal contents of more than 150 samples in Yangtze River Delta region were determined so as to make the soil environmental quality assessment and study the content distribution,transformation and distribution characteristics of heavy metals in different regions and different plant parts.[Result]The Yangtze River Delta region was polluted by Cd,Pb,Cr,Cu and Zn,among them Cd pollution was the most serious;Pb,Cu and Zn took second place;Cr appears the lightest;And there was almost no Hg and As pollution in this area.The Nemero pollution index of soil was 0.880 which was at the alert level.Cd,Cr,Cu,Hg and Zn in rice grains exceeded the background values.Heavy metal pollutions in soils divided by regions were in order of Taihu Lake region 〉Zhejiang region〉 Yangtze River region and urban areas 〉towns and rural areas.Heavy metal pollutions in soil divided by the uptake level were in order of rhizosphere soil 〉rice straw 〉rice grain.Transformation coefficients were in order of Cd〉 Cu 〉Zn〉 As 〉Hg〉 Pb〉 Cr.Distribution coefficients were in order of Zn〉 Cr〉 Cd 〉Cu〉 Hg 〉Pb 〉As.[Conclusion]There were larger value ranges of contaminated elements in the Yangtze River Delta Region,moreover individual samples had serious pollution,so the prevention and control of heavy metal pollution should be strengthened.

Study on the Occurrence and Epidemic Regularity and Region Division of Rice Blast in Nanchong City

With the aim to improve the level of monitoring and warning as well as the comprehensive control of rice blast disease, and to feasibly reduce the disease threat in Nanchong City, the methods of GPS and GIS, systematical monitoring and field survey, rice blast resistance identification, physiologic races of rice blast monitoring, and meteorological data analysis were performed to study the occurrence and epidemic region division, precise demarcation and occurrence and epidemic regularity of rice blast in Nanchong City. This study first completed the epidemic region division and precise demarcation; first clarified the initial affection（beginning period） locations, occurrence characteristics, epidemic trends and characteristics; explicated the existence of four epidemic peak periods of rice blast in the field, where the damage areas of first peak period played a decisive role during the blast epidemic years; in late May, the cumulative occurrence areas and annual occurrence areas presented higher positive correlation with the correlation coefficient of 0.817;and established a prediction model of occurrence areas per year based on the disease field rate at the end of boot stages and the diseased plant rate at dough stages. The results of investigation on the impact factors investigation of blast disease in Nanchong in recent years suggested that the internal causes were the decrease or loss of blast resistance of the rice cultivars, as well as the increase of physiological races with strong resistance to rice blast and the emergence of new virulent physiologic varieties; the external causes were suitable temperature, too much rainy, and sunlight shortage. Between 2010 and 2015, the short-term forecast accuracy for rice blast in Nanchong was up to 100%, and medium-and long-term forecast accuracy was also up to 98% and 95%, respectively, which increased by 5-15% than that before 1997, thereby making the control effect of rice blast in Nanchong increased by 15-30%.

Population Construction and Super-high Yield Characteristics of Japonica Super Rice in Cold Regions

[Objective] This study was conducted to construct a super-high yield population of Japnica rice in cold regions of North China and to explore its characteristics. [Method] The super rice variety Longjing 21 was selected as the experimental material. Different row spacing（two levels）, plant spacing（three levels） and seedling number per hill（three levels） were designed in field trials. Then, the growth stages,dry matter accumulation, leaf area, yield and yield components of these different treatments were measured. [Result] Rice yield had significantly negative correlation with plant spacing and row spacing, but no significant correlation with seedling number per hill. Rice yield was mainly affected by plant spacing, and less affected by seedling number per hill. The best recommended specifications for plant spacing of super rice variety Longjing 21 were 24 cm for row spacing, 12 cm for plant spacing and five seedlings per hill, and the expected yield was 10 473.0 kg/hm^2.The increased number of total spikelets（4.5×108hm2or more） in populations was the major reason for high yield. Super-high yield populations had fewer tillers at the early growth stage and achieved the expected number of productive tillers at critical leaf-age（June 25）, and tiller number peaked at jointing stage（July 6） and was about 1.2 times of the expected number. The percentage of productive tillers in total tillers of super-high yield population was more than 85% at heading stage. At middle growth stage（from jointing to heading stage）, the dry matter accumulation, leaf area index（LAI） at heading stage, effective leaf area, spikelet number of population,spikelet number per leaf area unit（cm2）, and culm-sheath weight per stem of super-high yield population were significantly higher than those of other populations.At late growth stage（from heading to maturity stage）, the leaf area decreasing rate of super-high yield population was significantly smaller than that of other populations. At late growth stage（from heading to maturity stage）, the leaf area decreasing rate, crop growth rate, net assimilation rate, biomass accumulation, number of filled grains per leaf area unit（cm^2） and grain weight per leaf area unit（cm^2） of high-yield population were significantly higher than those of other populations. Output and translocation of dry matter（weight per stem and sheath and total filling rate at maturity） from heading to milky stage of super-high yield population were significantly higher than those of other populations. [Conclusion] The characteristics of superhigh yield rice in cold region of North China are enriching the amount of actual filling of sink through improving photosynthetic efficiency from heading to maturity stage on the basis of enough panicle numbers.

Evaluating Varieties and Test Sites in the 2017 Rice Regional Trials of Hubei Province by GGE Biplot Based on Genstat

为分析水稻区试参试品种的丰产性、稳产性、适应性以及区试地点的代表力和鉴别力,采用Gen Stat软件中的GGE双标图对湖北省2017年水稻区试A组12个参试品种和10个区试地点进行了分析。结果表明,深两优10号、亮两优1212、隆晶优4393、襄优5327产量较高,亮两优1212、隆晶优4393、聚两优639、深两优10号具有较好的稳产性,襄优5327稳产性较弱,但在生产上仍有推广利用的价值。区试地点沙洋县、黄冈市、孝南区的代表力和鉴别力较强。

A Method for Upscaling Genetic Parameters of CERES-Rice in Regional Applications

To upscale the genetic parameters of CERES-Rice in regional applications, Jiangsu Province, the second largest rice producing province in China, was taken as an example. The province was divided into four rice regions with different rice variety types, and five to six sites in each region were selected. Then the eight genetic parameters of CERES-Rice, particularly the four parameters related to the yield, were modified and validated using the Trial and Error Method and the local statistical data of rice yield at a county level from 2001 to 2004, combined with the regional experiments of rice varieties in the province as well as the local meteorological and soil data （Method 1）. The simulated results of Method 1 were compared with those of other three traditional methods upscaling the genetic parameters, i.e., using one-site experimental data from a local representative rice variety （Method 2）, using local long-term rice yield data at a county level after deducting the trend yield due to progress of science and technology （Method 3）, and using rice yield data at a super scale, such as provincial, ecological zone, country or continent levels （Method 4）. The results showed that the best fitness was obtained by using the Method 1. The coefficients of correlation between the simulated yield and the statistical yield in the Method 1 were significant at 0.05 or 0.01 levels and the root mean squared error （RMSE） values were less than 9% for all the four rice regions. The method for upscaling the genetic parameters of CERES-Rice presented is not only valuable for the impact studies of climate change, but also favorable to provide a methodology for reference in crop model applications to the other regional studies.

Effect of Salt Stress on Nitrogen Assimilation of Functional Leaves and Root System of Rice in Cold Region

The aims were to investigate the effect of salt stress on key enzyme activity of nitrogen metabolism and the concentration of nitrate nitrogen and ammonium nitrogen response to salt stress.Two rice cultivars,Mudanjiang 30(sensitive cultivar)and Longdao 5(salt-tolerant cultivar),were treated with different salt concentrations(CK 0%,S10.075%,S20.15%,S30.225%and S40.3%).The results showed that the activities of nitrate reductase(NR),glutamine synthase(GS),glutamate synthase(GOGAT)and glutamate dehydrogenase(GDH)in the functional leaves and roots of rice in cold region presented a single peak curve change and the peak occurred in the heading stage;compared with those of the CK,the activities of NR,GS and GOGAT of rice in cold region decreased,but the activity of GDH increased in the heading stage under salt stress.The variation for key enzyme activity of nitrogen metabolism was the highest under S4 treatment.The activities of NR,GS and GOGAT in the functional leaves significantly decreased compared with those in roots;the concentrations of nitrate nitrogen and ammonium nitrogen in the functional leaves and roots of rice in cold region presented a single peak curve change and the peak occurred in the heading stage;compared with that of the CK,the concentration of nitrate nitrogen decreased in leaves and roots,the concentration of ammonium nitrogen decreased and the concentration of ammonium nitrogen in roots increased under salt stress.The variations for the activities of NR,GS and GOGAT in the functional leaves and roots of Longdao 5 were less than those of Mudanjiang 30 under the same concentration of salt stress.

Effects of Straw and Biochar Returned to the Soil on Soil Physical Properties and pH Value in Cold Rice Region

[Objectives]In order to explore the feasibility of using straw and biochar returned to the soil to improve soil physical properties and pH value in cold rice regions of China.[Methods]the effects of straw directly returned to the soil and charred straw(biochar)returned to the soil on soil bulk density,porosity,temperature and pH value of cold paddy soil were studied in this paper.[Results]The results showed that compared with conventional production,straw(6 t/ha),a small amount of biochar(2 t/ha)and a large amount of biochar(40 t/ha)returned to the soil reduced paddy soil bulk density at different growth stages by 6.02%-11.86%,2.69%-6.67%and 8.58%-11.32%,respectively,increased total porosity by 7.41%-14.93%,3.19%-8.38%and 9.81%-14.27%,respectively,and increased aeration porosity by 22.28%-192.11%,17.80%-92.11%and 52.44%-157.11%,respectively.Straw and a small amount of biochar returned to the soil had no significant effect on soil temperature and pH value of paddy field,but a large amount of biochar returned to the soil could significantly increase soil temperature by 5.13%-8.79%and pH value by 3.15%-5.96%in the later stage of rice growth.[Conclusions]The straw and biochar returned to the soil could reduce soil bulk density,increase total porosity and aeration porosity,and only a large amount of biochar returned to the soil could significantly increase soil temperature and pH value.

Standardized Cultivation of Cold Region Rice in Heilongjiang Reclamation Area

The paper analyzes the characteristics of rice production in Heilongjiang Reclamation Area(HRA)and the achievements made through innovative research and practice,evaluates the standardized mode of rice cultivation and technical standards for rice production,which play a key role in guaranteeing the growth of grain total output in Heilongjiang reclamation area and make great contributions to the national grain safety.

Design and Research on Virtual Display of Whole Process Mechanization for Rice Production in Cold Region

With the development of society,the virtual reality technology and multimedia technology have been applied to modern large-scale agriculture. The paper describes the design ideas for animation display of mechanized rice production in cold region,and elaborates the design of major part of each scene. By animation display,we can know about the whole process of rice production,as well as the benefits of mechanized operation.

A 48-bp deletion upstream of LIGULELESS 1 alters rice panicle architecture

Panicle architecture is an agronomic determinant of crop yield and a target for cereal crop improvement.To investigate its molecular mechanisms in rice,we performed map-based cloning and characterization of OPEN PANICLE 1(OP1),a gain-of-function allele of LIGULELESS 1(LG1),controlling the spread-panicle phenotype.This allele results from a 48-bp deletion in the LG1 upstream region and promotes pulvinus development at the base of the primary branch.Increased OP1 expression and altered panicle phenotype in chimeric transgenic plants and upstream-region knockout mutants indicated that the deletion regulates spread-panicle architecture in the mutant spread panicle 1(sp1).Knocking out BRASSINOSTEROID UPREGULATED1(BU1)gene in the background of OP1 complementary plants resulted in compact panicles,suggesting OP1 may regulate inflorescence architecture via the brassinosteroid signaling pathway.We regard that manipulating the upstream regulatory region of OP1 or genes involved in BR signal pathway could be an efficient way to improve rice inflorescence architecture.

Natural conditions and cropping regionalization of rice area in China

The formation of rice distribution is based on certain natural ecological conditions and social economic environments. In China, rice cropping is distributed in a vast area extending across 5 tempera ture belts, the northernmost of rice growing area in the world being in China. Distribution of rice cropping is characterized by a gradual decrease from south to north, from large and concentrated regions in Southeast to small and separated areas in Northwest. Natural conditions in rice regions differ in China with a varied topography, high in the west and low in the east. Rice fields in the west are mostly distributed on flatlands on plains, valleys among mountains, tablelands in river valleys, yellow-soil plains and basins in low valleys, alluvial plains, plains in river valleys, while those in the east are mainly distributed on alluvial plains, hilly areas among low mountains, coastal plains, along rivers and lakes and in basins among mountains.

Improvement of foreign gene expression in transgenic rice(Oryza sativa L.)by matrix attachment regions(MARs)

Matrix attachment regions or matrix associated regions (MARs) were special DNA sequences in chromatin of eukaryotic cells that tightly associated with the nuclear matrix or scaffold in vitro after a combination of nuclease digestion and extraction. They were also called scaffold attachment regions(SARs) . It was found that MARs could improve the expression level and the stability of foreign genes in transgenic plants. The reason might be that a transgene flanked by MARs was transmitted into the plant cells, the MARs would attach the nuclear

The East Asia regional meeting of International Program on rice biotechnology was held in CNRRI

Supported by the Rockefeller Foundation, the East Asia regional meeting of the international program on rice biotechnology was held in CNRRI Nov 5—8 1996. About 76 delegates from home and abroad attended the meeting and more than 80 papers were presented or posted. The objective of the meeting was to review the status of rice biotechnology since 1995 and to promote the international collaborative activities. The topic involved anther culture, gene isolation and characterization, transfer and expression of foreign genes, etc.

Mechanization of irrigated rice cropping regions in China

The mechanization of rice production in China has experienced a comparatively long and an undulating process. The full-scale making of mechanization of irrigated rice cropping regions was started at the mid 1960’s, later than that of upland cropping and its average level of mechanization operation also lower than the average level of the country. During this peiod, however, a series of mechines were created, which adapted to the characteristics of rice cropping regions in China, and the foundation was laid for the later paddy field machinery. For example, transplanting machine (created in 1956), boat—shaped tractors (1961), paddy field ploughing and harrowing serial (1974), and single wheel plowing machines (1979) etc.

CERES-Rice模型区域应用中遗传参数升尺度的一种方法

为了提升CERES-Rice模型中遗传参数的空间尺度,以适应区域性研究需要,以江苏省为例,在具有不同水稻品种生态类型的4个稻区各选5～6个样点,以稻区为空间尺度,利用每个样点4年(2001-2004年)县级统计水稻单产资料及同期同地的气象和土壤资料,结合江苏省各地的水稻品种区域试验资料,采用试错法对CERES-Rice中8个遗传参数,特别是4个与产量相关的遗传参数分别进行了调试与确定(方法1,简称稻区尺度调试法),并与传统的其他3种升尺度方法,即代表性品种单点调试法(方法2)、县级尺度调试法(方法3)和超大尺度调试法(方法4)的模拟效果进行了比较。结果显示,方法1的模拟效果较为理想,各稻区模拟产量与统计产量的相关系数均达到显著或极显著水平,均方根差值均小于9%。而其他3种方法的模拟效果均明显不如方法1。研究提出的遗传参数升尺度方法不仅适用于气候变化影响评价研究,也可为作物生长模型在其他区域研究中的应用提供方法上的借鉴。

站点CERES-Rice模型区域应用效果和误差来源

作物区域模拟是利用有限的空间数据,最大限度地反映出生育期、产量等作物性状的时空变化规律。由于目前的作物模型大多是田间尺度的站点模型,把它运用到区域水平的效果如何研究甚少。文章利用CERES-Rice模型,对作物模型在我国的区域应用效果进行了分析。首先利用田间观测数据在各实验点上对模型进行了详细的站点校准,以验证模型在我国的模拟能力;然后以我国水稻生态区(精确到亚区)为单位,运用均方根差(RMSE)法进行了区域校准和验证;最后利用区域校准后的CERES-Rice模型,模拟了1980～2000年的网格(50km×50km)水稻产量,并与同期农调队调查产量进行统计比较,以验证区域应用的效果,为区域模拟的推广和应用提供参考。结果表明:经过空间校准后的CERES-Rice模型,在水稻的主产区1～4区(占种植面积的95%)模拟的平均产量与调查产量相对均方根差在22%以内,两者的符合度也较好,个别区域(5、6)RMSE%在24%～30%之间;1980～2000年水稻各产区模拟的平均产量与调查产量随时间变化趋势也具有一定的一致性;全国1896个网格中,大部分网格(71.01%)模拟的21年水稻年产量与调查产量的RMSE%在30%之内,且大部分分布在水稻主产区,考虑到水稻种植面积的权重后,认为利用区域校准和验证后的CERES-Rice模型进行水稻区域模拟,可以反映出产量的时空分布特征,能够为宏观决策提供相应的信息。但目前区域模拟中还存在着一定的误差,有待今后进一步研究。

DICE/RICE模型中碳循环模块的比较

碳循环模型的正确构建是影响综合集成评估模型IAM(Integrated Assessment Model)模拟结果的重要因素之一。DICE/RICE模型中的碳循环模型主要有两个,即Nordhaus单层碳库模型和Nordhaus三层碳库模型,但这两个模型的主要缺陷是不考虑陆地生态系统在碳循环中的贡献,因此,引入了包含陆地生态系统的Svirezhev碳循环模型,并将其与Nordhaus单层碳库模型、Nordhaus三层碳库模型展开比较研究。结果表明,在基于历史数据的模型检验中,Svirezhev碳循环模型对全球二氧化碳浓度模拟的准确度优于其他两个模型。对于未来全球气候变化的模拟,3个模型模拟得到了至2100年的温度预测值分别为2.98,3.54,2.91℃,二氧化碳浓度值分别为608.04,733.04,594.70μL/L。其中,Svirezhev碳循环模型的模拟值在3个模型中最低,表明了陆地生态系统和海洋对二氧化碳的吸收作用对抑制全球升温的贡献;而分析也发现Nordhaus三层碳库模型对陆地生态系统和海洋碳库的模拟与实际观测值偏离较大。最后,通过敏感性分析,研究发现DICE/RICE模型中使用的气候响应模块在短期温度模拟中对地表温度的初值较为敏感,在长期温度模拟中敏感度显著下降。总之,从碳循环机制的模拟性能而言,Svirezhev碳循环模型优于其他两个模型,而Nordhaus单层碳库模型虽然机制较为简单却保证了模拟的准确性,但Nordhaus三层碳库模型虽然丰富了碳库的表征,实际上各碳库的模拟准确性差,降低了模型的可靠性。

Characterization of Reverse Thermo-sensitive Genic Male Sterile Lines in Cold Regions of China

Yannong S, a reverse thermo-sensitive genic male sterile （TGMS） line, is sterile at high temperature and fertile at low temperature. In the present study, the fertility of Yannong S and the japonica reverse TGMS lines derived from it was in- vestigated in Harbin, Heilongjiang from 2013 to 2015. The results showed that Yan- nong S was completely male sterile in July and August （the hottest months of the year in Harbin）, and the seed setting rate of bagged panicles was 0. We also in- ferred that the critical temperature for inducing the sterility of Yannong S was higher than 28 ℃. Most of the japonica reverse TGMS lines derived from Yannong S were completely aborted in July and August, with a seed setting rate of 0. It can be con- cluded that the reverse TGMS lines derived from Yannong S are genetically stable lines that have a long period of sterility in cold regions, so they may be the ideal genetic materials for the development and in-situ seed production of japonica hybrid rice.

Review and Prospects on Rice Breeding and Extension in China

A large number of new varieties, especially hybrid rice, have been developed and released in China since 1998. The yield potential and grain quality of hybrid rice have also been improved significantly both for three-line and two-line hybrid rice including indica and japonica types as well. The trends on extension of rice varieties in China since 1986 has been marked by the increasing number of new varieties, decreasing annual planting area per variety, decreasing number of varieties with a large-scale extension area, while both the renewal rate and the proportion of core varieties with cross-provincial extension have remained unchanged. This current major problems and future goals of rice breeding and extension in China were also discussed.