Objective:To analyze the effective chemical components and action targets of Phellodendron chinense,and to study the mechanism of Phellodendron chinense in treating ureteral stones.Methods:Search the potential chemica...Objective:To analyze the effective chemical components and action targets of Phellodendron chinense,and to study the mechanism of Phellodendron chinense in treating ureteral stones.Methods:Search the potential chemical active substances of Phellodendron chinense and target proteins acting on the human body through the TCMSP database,and use the genomic annotation database platform(Genecards)to predict the target of ureteral stones,and use the uniprot database Query the corresponding gene names,build a network diagram of"drug-disease-target"with the help of Cytoscape(3.7.2)software,build a protein interaction network through the String database platform,and then use the Bioconductor platform and R language GO Enrichment analysis and KEGG enrichment analysis.Results:Through screening,a total of 36 effective chemical constituents of Phellodendron chinense,8 key chemical constituents related to ureteral calculi,and 20 common targets of Phellodendron-ureteral calculi were obtained.The core genes of PPI were FOS,CXCL8,IL6,SERPINE1,VEGFA,EGF,NOS3,SPP1,CCL2,CCND1,EGFR,HMOX1,IL10,CDKN1A,CRP;obtained 36 GO biological processes,and 14 KEGG related signaling pathways,including HIF-1 signaling pathway,FoxO signaling pathway,EGFR tyrosine kinase inhibitor resistance,endocrine resistance,viral protein interaction with cytokines and cytokine receptors,calcium signaling pathway,p53 signaling pathway,ErbB signaling pathway,MAPK signaling pathway,Ras signaling pathway.Conclusion:Phellodendron can achieve the effect of treating ureteral calculi through multiple targets and multiple ways,which provides a theoretical basis for the future extraction of effective components to treat ureteral calculi.展开更多
At the end of the Ming Dynasty and the beginning of the Qing Dynasty,the Chinese Chan Master Yinyuan(隐元)traveled to Japan to teach the Buddhist Dharma and later founded the Japanese Obaku Sect(Huangbo Zong黄檗宗)of ...At the end of the Ming Dynasty and the beginning of the Qing Dynasty,the Chinese Chan Master Yinyuan(隐元)traveled to Japan to teach the Buddhist Dharma and later founded the Japanese Obaku Sect(Huangbo Zong黄檗宗)of Chan Buddhism.This essay is a brief introduction to Yinyuan’s life,the people and events related to the establishment of the Japanese Obaku Sect and the Chan methods he taught.展开更多
基金Beijing Traditional Chinese Medicine Technology Development Funding(No.QN2018-11)
文摘Objective:To analyze the effective chemical components and action targets of Phellodendron chinense,and to study the mechanism of Phellodendron chinense in treating ureteral stones.Methods:Search the potential chemical active substances of Phellodendron chinense and target proteins acting on the human body through the TCMSP database,and use the genomic annotation database platform(Genecards)to predict the target of ureteral stones,and use the uniprot database Query the corresponding gene names,build a network diagram of"drug-disease-target"with the help of Cytoscape(3.7.2)software,build a protein interaction network through the String database platform,and then use the Bioconductor platform and R language GO Enrichment analysis and KEGG enrichment analysis.Results:Through screening,a total of 36 effective chemical constituents of Phellodendron chinense,8 key chemical constituents related to ureteral calculi,and 20 common targets of Phellodendron-ureteral calculi were obtained.The core genes of PPI were FOS,CXCL8,IL6,SERPINE1,VEGFA,EGF,NOS3,SPP1,CCL2,CCND1,EGFR,HMOX1,IL10,CDKN1A,CRP;obtained 36 GO biological processes,and 14 KEGG related signaling pathways,including HIF-1 signaling pathway,FoxO signaling pathway,EGFR tyrosine kinase inhibitor resistance,endocrine resistance,viral protein interaction with cytokines and cytokine receptors,calcium signaling pathway,p53 signaling pathway,ErbB signaling pathway,MAPK signaling pathway,Ras signaling pathway.Conclusion:Phellodendron can achieve the effect of treating ureteral calculi through multiple targets and multiple ways,which provides a theoretical basis for the future extraction of effective components to treat ureteral calculi.
文摘At the end of the Ming Dynasty and the beginning of the Qing Dynasty,the Chinese Chan Master Yinyuan(隐元)traveled to Japan to teach the Buddhist Dharma and later founded the Japanese Obaku Sect(Huangbo Zong黄檗宗)of Chan Buddhism.This essay is a brief introduction to Yinyuan’s life,the people and events related to the establishment of the Japanese Obaku Sect and the Chan methods he taught.
