Study on Physiological Characteristics and Supporting Techniques of Huayu 22 Peanut

Huayu 22 Peanut( Arachis hypogaea L.) belongs to ordinary large variety of peanut with high quality,high yield potential and high stress resistance. Study on nutrition characteristics,stress physiology and supporting techniques of Huayu 22 Peanut is conducive to further developing its application value in breeding and exploiting its high yield potential. Compared with Baisha 1016 Peanut,Huayu 22 Peanut has greater shade tolerance. In weak light,both the leaf area and rate of photosynthesis of Huayu 22 Peanut decline slightly,and damage of chloroplast ultramicrostructure is less,light compensation point and chloroplast a / b are lower,and capturing and utilization efficiency of weak light are higher; after removal of shade,Huayu 22 Peanut plant gets recovered quickly with higher growth compensation effect but slight dropping of yield. Characteristics of N supply for Huayu 22 Peanut at different growth stages are as follows: N supply intensity of root nodule takes on unimodal curve with peak at pod bearing stage. The N supply intensity of soil takes on N shape with higher value at pegging stage and pod filling stage; N supply intensity takes on unimodal curve with peak at pegging stage. At seedling stage and pod filling stage,N is mainly supplied by soil; at pegging stage and pod bearing stage,N is supplied by root nodule. At whole growth stage,root nodule has the largest contribution to N supply,accounting for 50. 0%,followed by soil and fertilizer,and the proportion is about 5: 3: 2. The study established standardized supporting technique for simple cultivation of Huayu 22 Peanut with " single-seed planting,high effective fertilizer application,and three sprays and multi-prevention".

清肠化瘀方对溃疡性结肠炎患者CDX2 mRNA、miR-22及Th17细胞的影响

目的探讨中药清肠化瘀方对溃疡性结肠炎(ulcerative colitis,UC)患者尾型同源盒转录因子2(caudal type homeobox transcription factor2,CDX2)、miR-22及辅助性T细胞17(the helper 17 cells,Th17)的影响。方法选择大肠湿热型UC患者96例,按照随机数字表法分为研究组(48例)和对照组(48例),研究组口服美沙拉嗪片和中药清肠化瘀方,对照组口服美沙拉嗪片和中药清肠化瘀方低剂量配方颗粒,疗程均为12周。比较两组治疗前后中医证候评分、Sutherland疾病活动指数(disease activity index,DAI)评分、Geboe指数评分;并包括两组血清CDX2 mRNA、miR-22、IL-17、IL-21水平和外周血Th17含量,比较炎症性肠病生活质量问卷(inflammatory bowel disease quality of life questionnaire,IBDQ)评分。结果治疗后研究组中医证候评分、DAI评分、Geboe指数评分均低于对照组(P<0.01);血清CDX2 mRNA水平高于对照组(P<0.01),miR-22水平低于对照组(P<0.01),外周血Th17细胞含量及血清IL-17、IL-21水平低于对照组(P<0.01);IBDQ各维度(肠道症状、全身症状、情感能力、社会能力)评分均高于对照组(P<0.01)。结论清肠化瘀方可有效改善中医证候,促进肠黏膜的修复和愈合,改善患者生活质量,治疗大肠湿热型UC效果显著,其机制可能与调控CDX2 mRNA、miR-22表达,抑制Th17细胞分化,减少IL-17、IL-21等Th17细胞相关因子合成有关。

弱光胁迫对花生功能叶片RuBP羧化酶活性及叶绿体超微结构的影响

间作套种是我国主要的花生(Arachis hypogaea)种植方式之一。然而,与单作相比,在间作套种体系中,花生截获的光能较少,生长发育差,产量低,研究不同品种耐阴机理对选择适宜间作套种的花生品种具有重要意义。该研究用耐阴性不同的两个花生品种‘花育22号’(强耐阴性)和‘白沙1016’(弱耐阴性)为材料,在大田条件下采用不同透光率遮阴网设置50%自然光强(中度弱光胁迫)和15%自然光强(严重弱光胁迫)2个弱光处理,从出苗期开始遮阴40天,以自然光强为对照,研究了弱光胁迫对花生功能叶片RuBP羧化酶活性和叶绿体超微结构的影响。结果表明:光强为自然光照50%和15%的处理,‘花育22号’RuBP羧化酶活性与对照相比虽有降低,但差异不显著,而‘白沙1016’分别比对照低40.1%和59.4%,显著低于对照。与对照相比,50%自然光强下‘花育22号’叶绿体数不变,叶绿体基粒数和基粒片层数显著增多,叶绿体变长且发育完好,15%自然光强下,叶绿体数、基粒数和淀粉粒数显著减少,叶绿体膜和基粒片层出现破损,但叶绿体变长,基粒片层数增加;‘白沙1016’在50%自然光强下,叶绿体数目和超微结构变化同‘花育22号’相似,在15%自然光强下叶绿体变圆,基粒数的降幅和基粒片层破损程度大于‘花育22号’且基粒片层数减少,淀粉粒数增多。因此,弱光胁迫特别是严重弱光胁迫条件下,功能叶RuBP羧化酶活性降低幅度小、叶绿体超微结构受损程度低是‘花育22号’耐阴的光合生理基础。