In order to investigate the feasibility of BDS/GPS in the deformation monitoring of long-span bridges,analysis and research on aspects like number of visible satellites,PDOP value and monitoring precision are carried ...In order to investigate the feasibility of BDS/GPS in the deformation monitoring of long-span bridges,analysis and research on aspects like number of visible satellites,PDOP value and monitoring precision are carried out. To analyze daily deformation characteristics of steel box girder bridge,observation data for 48 consecutive hours is computed by self-programmed software. Experiment results show that the monitoring-points on the bridge demonstrate obvious periodicity and recoverability in vertical and horizontal directions,meanwhile,changes in the elevation direction are relatively stable. The deformation-monitoring results of BDS/GPS combination system and GPS single system show good consistency. However,in a complex environment of the bridge,especially under the condition that satellite signals are severely affected,the advantages of BDS/GPS combination over GPS single system are more obvious.展开更多
Pure laparoscopic hepatectomy is a less invasive procedure than conventional open hepatectomy for the resection of hepatic lesions. Increases in experiences with the technique, in combination with advances in technolo...Pure laparoscopic hepatectomy is a less invasive procedure than conventional open hepatectomy for the resection of hepatic lesions. Increases in experiences with the technique, in combination with advances in technology, have promoted the popularity of pure laparoscopic hepatectomy. However, indications for usage and potential contraindications of the procedure remain unresolved. The characteristics and specific advantages of the procedure, especially for hepatocellular carcinoma(HCC) patients with chronic liver diseases,are reviewed and discussed in this paper. For cirrhotic patients with liver tumors, pure laparoscopic hepatectomy minimizes destruction of the collateral blood and lymphatic flow from laparotomy and mobilization, and mesenchymal injury from compression. Therefore, pure laparoscopic hepatectomy has the specific advantage of minimal postoperative ascites production that leads to lowering the risk of disturbance in water or electrolyte balance and hypoproteinemia. It minimizes complications that routinely trigger postoperative serious liver failure. Under adequate patient positioning and port arrangement, the partial resection of the liver in the area of subphrenic space, peri-inferior vena cava area or next to the attachment of retro-peritoneum is facilitated in pure laparoscopic surgery by providing good vision and manipulation in the small operative field.Furthermore, the features of reduced post-operative adhesion, good vision, and manipulation within the small area between the adhesions make this procedure safer in the context of repeat hepatectomy procedures.These improved features are especially advantageous for patients with liver cirrhosis and multicentric and/or metachronous HCCs.展开更多
根据犬瘟热病毒(C an ine d istem per v irus,CDV)融合蛋白(F)的基因序列,利用L earnCo il-VM F与ExPA Sy软件预测出两个七肽重复区(heptad repeat,HR 1与HR 2),应用搭桥PCR拼接的方法获得HR 1与HR 2基因,将其直接克隆到pGEX-6p-I表达...根据犬瘟热病毒(C an ine d istem per v irus,CDV)融合蛋白(F)的基因序列,利用L earnCo il-VM F与ExPA Sy软件预测出两个七肽重复区(heptad repeat,HR 1与HR 2),应用搭桥PCR拼接的方法获得HR 1与HR 2基因,将其直接克隆到pGEX-6p-I表达载体构建重组质粒,用PCR及双酶切方法鉴定阳性重组质粒,并对其进行测序鉴定。并在大肠杆菌中进行融合表达。展开更多
基金Supported by the National Natural Science Foundation of China(No.41604018)Research Project of the Production and Research Institute of Jiangsu Province(No.2015002-04)
文摘In order to investigate the feasibility of BDS/GPS in the deformation monitoring of long-span bridges,analysis and research on aspects like number of visible satellites,PDOP value and monitoring precision are carried out. To analyze daily deformation characteristics of steel box girder bridge,observation data for 48 consecutive hours is computed by self-programmed software. Experiment results show that the monitoring-points on the bridge demonstrate obvious periodicity and recoverability in vertical and horizontal directions,meanwhile,changes in the elevation direction are relatively stable. The deformation-monitoring results of BDS/GPS combination system and GPS single system show good consistency. However,in a complex environment of the bridge,especially under the condition that satellite signals are severely affected,the advantages of BDS/GPS combination over GPS single system are more obvious.
文摘Pure laparoscopic hepatectomy is a less invasive procedure than conventional open hepatectomy for the resection of hepatic lesions. Increases in experiences with the technique, in combination with advances in technology, have promoted the popularity of pure laparoscopic hepatectomy. However, indications for usage and potential contraindications of the procedure remain unresolved. The characteristics and specific advantages of the procedure, especially for hepatocellular carcinoma(HCC) patients with chronic liver diseases,are reviewed and discussed in this paper. For cirrhotic patients with liver tumors, pure laparoscopic hepatectomy minimizes destruction of the collateral blood and lymphatic flow from laparotomy and mobilization, and mesenchymal injury from compression. Therefore, pure laparoscopic hepatectomy has the specific advantage of minimal postoperative ascites production that leads to lowering the risk of disturbance in water or electrolyte balance and hypoproteinemia. It minimizes complications that routinely trigger postoperative serious liver failure. Under adequate patient positioning and port arrangement, the partial resection of the liver in the area of subphrenic space, peri-inferior vena cava area or next to the attachment of retro-peritoneum is facilitated in pure laparoscopic surgery by providing good vision and manipulation in the small operative field.Furthermore, the features of reduced post-operative adhesion, good vision, and manipulation within the small area between the adhesions make this procedure safer in the context of repeat hepatectomy procedures.These improved features are especially advantageous for patients with liver cirrhosis and multicentric and/or metachronous HCCs.
文摘根据犬瘟热病毒(C an ine d istem per v irus,CDV)融合蛋白(F)的基因序列,利用L earnCo il-VM F与ExPA Sy软件预测出两个七肽重复区(heptad repeat,HR 1与HR 2),应用搭桥PCR拼接的方法获得HR 1与HR 2基因,将其直接克隆到pGEX-6p-I表达载体构建重组质粒,用PCR及双酶切方法鉴定阳性重组质粒,并对其进行测序鉴定。并在大肠杆菌中进行融合表达。
