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Effect of Baicalein on the Expression of VIP in Extravillous Cytotrophoblasts Infected with Human Cytomegalovirus In Vitro 被引量:6
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作者 乔媛 方建国 +4 位作者 肖娟 刘涛 刘静 张艳丽 陈素华 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2013年第3期406-411,共6页
Summary: This paper aimed to study the ability of baicalein to block human cytomegalovirus (HCMV) infection in extravillous cytotrophoblasts (EVT) and its effect on the vasoactive intestinal peptide (VIP) expre... Summary: This paper aimed to study the ability of baicalein to block human cytomegalovirus (HCMV) infection in extravillous cytotrophoblasts (EVT) and its effect on the vasoactive intestinal peptide (VIP) expression in HCMV-infected EVT in vitro. A human trophoblast cell line (HPT-8) was chosen in this study. HCMV with 100 TCIDs0was added into culture medium to infect HPT-8 cells, and then HCMV pp65 antigen was assayed by immunofluorescence staining. The infection status was determined by vi- rus titration. Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect virus DNA load in the infected cells. The expression of VIP mRNA and protein in the infected cells was measured by qRT-PCR, immunocytochemistry and Western blotting. Concentration of VIP secreted in supernatants was determined by ELISA. Red-stained HCMV pp65 antigens were found in infected HPT-8 cells 48 h after infection. HCMV replicated in large quantity in infected HPT-8 cells 4 days after infection, reaching a peak at day 6 post-infection. After treatment with baicalein, virus DNA load in in- fected HPT-8 cells was decreased (P〈0.05), and the levels of VIP mRNA and protein, and the concen- tration were raised to the normal (P〉0.05). Our study suggested that baicalein exerts a positive effect on the VIP expression in HCMV-infected EVT at maternal-fetal interface. 展开更多
关键词 BAICALEIN human cytomegalovirus extravillous cytotrophoblasts vasoactive intestinal pep- tide
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Human Cytomegalovirus UL138 Open Reading Frame Is Highly Conserved in Clinical Strains 被引量:6
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作者 Ying Qi Rong He Yan-ping Ma Zheng-rong Sun Yao-hua Ji Qiang Ruan 《Chinese Medical Sciences Journal》 CAS CSCD 2009年第2期107-111,共5页
Objective To investigate the variability of human cytomegalovirus (HCMV) UL138 open reading frame (ORF) in clinical strains. Methods HCMV UL138 ORF was amplified by polymerase chain reaction (PCR) and PCR amplif... Objective To investigate the variability of human cytomegalovirus (HCMV) UL138 open reading frame (ORF) in clinical strains. Methods HCMV UL138 ORF was amplified by polymerase chain reaction (PCR) and PCR amplification products were sequenced directly, and the data were analyzed in 19 clinical strains. Results LIL138 ORF in all 30 clinical strains was amplified successfully. Compared with that of Toledo strain, the nucleotide and amino acid sequence identifies of LIL138 ORF in all strains were 97.41% to 99.41% and 98.24% to 99.42%, respectively. All of the nucleofide mutations were substitutions. The spatial structure and post-translational modification sites of HL138 encoded proteins were conserved. The result of phylogenetic tree showed that HCMV HL138 sequence variations were not definitely related with different clinical symptoms. Conclusion HCMV UL138 ORF in clinical strains is high conservation, which might be helpful for UL138 encoded protein to play a role in latent infection of HCMV. 展开更多
关键词 human cytomegalovirus UL 138 CONSERVATION
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SEQUENCE VARIABILITY OF HUMAN CYTOMEGALOVIRUS UL144 OPEN READING FRAME IN LOW-PASSAGE CLINICAL ISOLATES 被引量:6
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作者 RongHe QiangRuan ChangXia Lan-qingLiu Sheng-minLǔ YingLu YingQi Yan-pingMa QingLiu Yao-huaJi 《Chinese Medical Sciences Journal》 CAS CSCD 2004年第4期293-297,共5页
Objective To explore the relationship between human cytomegalovirus (HCMV) UL144 sequence variability and clinical disease. Methods HCMV UL144 open reading frame (ORF) was amplified by PCR assay in 72 lowpassage isola... Objective To explore the relationship between human cytomegalovirus (HCMV) UL144 sequence variability and clinical disease. Methods HCMV UL144 open reading frame (ORF) was amplified by PCR assay in 72 lowpassage isolates [65 con-genitally infective children and 7 healthy children who were HCMV-DNA positive by quantitative PCR (qPCR)]. All positive PCR products were analyzed by heteroduplex mobility assay and single-stranded conformation polymorphism (HMA-SSCP) and 32 of them were sequenced. Results Fifty-five patient isolates and five healthy children isolates were HCMV-UL144 positive by PCR. Sequencing and HMA-SSCP analysis showed that significant strain-specific variability was present in the UL144 ORF. Phylogenetic analysis indicated that the nucleotide sequences could be separated into 3 major genotypes. Comparing between UL144 se-quences and the corresponding symptoms showed that genotype 2 did not exist in megacolon isolates. And genotype 1 and 3 were the major types among microcephaly and jaundice isolates respectively. Conclusions HCMV-UL144 existed in most of low passage isolates and sequences were hypervariable. The UL144 ORF and its predicted product with the high level of sequence variability in different kinds of isolates suggest that UL144 ORF might play a role in HCMV infectivity and subsequent diseases. 展开更多
关键词 human cytomegalovirus UL144 open reading frame VARIABILITY
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Effect of Human Cytomegalovirus Infection on Nerve Growth Factor Expression in Human Glioma U251 Cells 被引量:3
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作者 HAI-TAO WANG BIN WANG +6 位作者 ZHI-JUN LIU ZHI-QIANG BAI LING LI HAI-YAN LIU DONG-MENG QIAN ZHI-YONG YAN XU-XIA SONG 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2009年第4期354-358,共5页
Objective To explore the change of endogenic nerve growth factor (NGF) expression in human glioma cells infected with human cytomegalovirus (HCMV). Methods U251 cells were cultured in RPMI 1640 culture medium and ... Objective To explore the change of endogenic nerve growth factor (NGF) expression in human glioma cells infected with human cytomegalovirus (HCMV). Methods U251 cells were cultured in RPMI 1640 culture medium and infected with HCMV AD 169 strain in vitro to establish a cell model of viral infection. Morphologic changes of U251 cells were observed under inverted microscope before and after infection with HCMV. Expression of NGF gene and protein of cells was detected by RT-PCR and Western blotting before and after infection with HCMV. Results The cytopathic effects of HCMV-infected cells appeared on day 5 after infection. However, differential NGF expression was evident on day 7. NGF expression was decreased significantly in U251 cells on day 7 after infection in comparison with control group (P〈0.05). Conclusion HCMV can down-regulate endogenous NGF levels in human glioma cell line U251. 展开更多
关键词 human cytomegalovirus U251 cells Nerve growth factor
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Human Cytomegalovirus Infection Inhibits the Differentiation of Human Hippocampus Neural Precursor Cells into Astrocytes 被引量:2
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作者 Ling LI Bin WANG +8 位作者 Peng LI Zhi-qiang BAI Hai-tao WANG Hai-yan LIU Dong-meng QIAN Xiu-xia SONG Shou-yi DING Zhi-yong YAN Guang-yu JIANG 《Virologica Sinica》 SCIE CAS CSCD 2009年第6期552-558,共7页
HCMV is a major cause of congenital brain disease in humans, and its neuropathogenesis is not yet fully understood. The objective of the present study is to investigate the effect of human cytomegalovirus (HCMV) infec... HCMV is a major cause of congenital brain disease in humans, and its neuropathogenesis is not yet fully understood. The objective of the present study is to investigate the effect of human cytomegalovirus (HCMV) infection on human hippocampus neural precursor cell (NPCs) differentiation in vitro. Fetal hippocampus tissue was dissociated mechanically and then cultured in proliferation medium with EGF and bFGF. The identification and purity of the NPCs were confirmed by using immunofluorescence to detect the expression of the NPCs marker-Nestin. To drive NPCs differentiation, bFGF and EGF were withdrawn from the medium and replaced with FBS (10%). HCMV AD169 (MOI=5) was added into the differentiation medium at the onset of the differentiation. After 7 days of differentiation, in order to confirm whether NPCs are permissive for HCMV infection, immunofluorescence was used to stain for the presence of immediate early (IE) and late (pp65) HCMV proteins in the infected cells. The effects of HCMV infection on NPCs’ differentiation was observed by detecting the ratio of nestin and GFAP positive cells with confocal microscopy and immunofluorescence. The data showed that 95%±8% of the cells (passage 4-8) cultured were Nestin positive which suggested that majority of the cells were NPCs. On day 7 postinfection, most of the infected cells were IE and PP65 positive. The percentage of Nestin-positive cells were 93%±10% and 50%±19% (t=6.03, p<0.01) and those of GFAP-positive cells were 55±17% and 81%±11% (t=3.77, p<0.01) in HCMV treated and control groups respectively. These findings indicate that NPCs are HCMV permissive cells and HCMV (AD 169) infection suppresses the differentiation of Hippocampus-genetic human NPCs into astrocytes. These effects may provide part of the explanation for the abnormalities in brain development associated with congenital HCMV infection. 展开更多
关键词 human cytomegalovirus (HCMV) Neural stem cells Cell differentiation HIPPOCAMPUS NESTIN
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Effect of Human Cytomegalovirus on Invasive Capability of Early Pregnant Extravillous Cytotrophoblasts 被引量:2
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作者 刘涛 郑晓菲 +6 位作者 陈娟娟 王楠 肖娟 张丹丹 尹宗智 李伟 陈素华 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2011年第6期819-823,共5页
The effect of human cytomegalovirus (HCMV) on invasive capability of early pregnant extravillous cytotrophoblasts (EVTs) was investigated in vitro. Primary EVTs were obtained by complex phosphoesterasum digestion ... The effect of human cytomegalovirus (HCMV) on invasive capability of early pregnant extravillous cytotrophoblasts (EVTs) was investigated in vitro. Primary EVTs were obtained by complex phosphoesterasum digestion and gradient centrifugation from villous tissue aseptically taken from healthy pregnant women. Cytokeratin7 (CK7), vimentin (Vim) and cerbB-2 were immunocytochemically detected to identify source of cells, and HCMVpp65 antigen was assayed to determine the infection state of primary EVTs by immunocytochemical staining. The EVTs were divided into two groups: control group and HCMV group, and the expression of c-erbB-2, matrix metalloproteinase-2 (MMP-2) and MMP-9 proteins was detected in two groups by immunocytochemistry and Western blotting. Enzymic activity changes of MMP-2 and MMP-9 were tested by gelatin zymography in primary EVTs infected with HCMV. The invasion of primary EVTs was detected by cell invasion assay in vitro after they were infected by HCMV. The cell source identification showed that the cells obtained were highly-pure primary EVTs, and primary EVTs could be infected by HCMV. Primary EVTs could express c-erbB-2, MMP-2 and MMP-9 proteins, and as compared with control group, the protein expression was decreased significantly in HCMV groups (P〈0.05). Primary EVTs could secrete active MMP-2 and MMP-9 in vitro, and the activity of two MMPs was decreased sig- nificantly in HCMV groups (P〈0.05). The in vitro cell invasion assay showed that the number of primary EVTs permeating Matrigel in HCMV group was decreased (P〈0.05). We are led to conclude that HCMV can infect primary EVTs and inhibit their invasion capability, suggesting that the im- paired EVT's invasion capability might be related to the abnormal expression of c-erbB-2, MMP-2 and MMP-9 proteins. 展开更多
关键词 human cytomegalovirus extravillous cytotrophoblast C-ERBB-2 MMP-2 MMP-9 INVASION
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Effects of Human Cytomegalovirus Infection on Apoptosis and Expression of Apoptosis-Regulating Factors 被引量:2
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作者 聂兴草 方峰 +5 位作者 李红 董永绥 周玉峰 甄宏 刘志峰 李革 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2005年第5期480-483,共4页
Summary: The present study aimed to find out dynamic changes of apoptosis in human cytomegalovirus (HCMV) infected cells and the influence of HCMV infection on activation of caspase-3 and the expression of apoptosi... Summary: The present study aimed to find out dynamic changes of apoptosis in human cytomegalovirus (HCMV) infected cells and the influence of HCMV infection on activation of caspase-3 and the expression of apoptosis-regulating genes, bcl-2 and fas mRNA. The sequential changes of apoptotic cell rate in high and low MOI (MOI=2.5 and 0.25 respectively) of HCMV infected human embryonic lung fibroblasts (HELFs) at 1 h, 12 h, 24 h, 36 h, 48 h, 72 h and 96 h post-infection were measured by flow cytometry. The expression levels of caspase-3 protein and bcl-2 and fas mRNA in HCMV infected cells (MOI=0.25) at 72 h post-infection were detected by Western blot and in situ hybridization methods, respectively. It was found that the ratio of apoptotic cells in normal controls was consistently lower, but the rates in low and high MOI infected cells were gradually increased with time prolonged, reached peak at 96 h (8.85 %) and 72 h (25.63 %), respectively. By Western blot analysis, only a narrow band of 32 kD (1 kD=0. 992 1 ku) procaspase-3 was found in normal cells, but a wider procaspase-3 band and a much wider band of 17 kD proteins (p17) ap- peared in the infected cells. Meanwhile, the expression of bcl-2 mRNA was higher and that of fas mRNA was lower in the normal HELF cells, whereas there were significantly lower bcl-2 mRNA and higher fas mRNA expression levels in HCMV infected cells. It was concluded that HCMV was a stronger inducer of apoptosis in HELF cells. Caspase-3, as the marker of undergoing apoptosis, was expressed increasingly and activated in the infected cells, indicating its action in HCMV-inducing apoptosis. Down-regulating bcl-2 mRNA expression and up-regulating fas mRNA expression were also involved in the mechanism of HCMV-induced apoptosis. 展开更多
关键词 human cytomegalovirus APOPTOSIS apoptosis-regulating gene CASPASE
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Recombinant Human IgG antibodies against Human Cytomegalovirus 被引量:1
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作者 TAO DUAN XIAO-FANG WANG +2 位作者 SHU-YUAN XIAO SHU-YAN GU AND MI-FANG LIANG 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2008年第5期372-380,共9页
Objective To study the passive immunization with human monoclonal antibodies as for prophylaxis of human cytomegalovirus (HCMV) infection. Methods Fab monoclonal antibodies to HCMV were recovered by repertoire cloni... Objective To study the passive immunization with human monoclonal antibodies as for prophylaxis of human cytomegalovirus (HCMV) infection. Methods Fab monoclonal antibodies to HCMV were recovered by repertoire cloning of mRNA from a HCMV infected individual. Antigen binding specificity, CDR sequence of VH and VL and neutralizing activity on HCMV AD169 stain were analyzed in vitro. The light and heavy chain Fd fragment genes of Fab antibodies were further cloned into a recombinant baculovirus expression vector pAC-K-Fc to express intact IgG. Secreted products were purified with affinity chromatography using protein G. Results SDS-PAGE and Western blot confirmed the expression of the intact IgG. Immuno-blotting and -precipitation were used to identify HCMV proteins. One Fab monoclonal antibody recognized a conformational HCMV protein. Conclusion IgG antibodies can neutralize the HCMV AD169 strain efficiently at a titer of 2.5 μg/mL and may prove valuable for passive immunoprophylaxis against HCMV infection in humans. 展开更多
关键词 human cytomegalovirus human engineering antibody Phage display Recombinant baculovirus expression
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SEQUENCE CONSERVATION OF HUMAN CYTOMEGALOVIRUS UL140 OPEN READING FRAME IN CLINICAL STRAINS 被引量:1
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作者 Ying Qi Yan-ping Ma Rong He Zheng-rong Sun Yao-hua Ji Yu-jing Huang Qiang Ruan 《Chinese Medical Sciences Journal》 CAS CSCD 2008年第1期23-27,共5页
Objective To investigate the variability of human cytomegalovirus (HCMV) UL140 open reading flame (ORF) in clinical strains, and to explore the relationship between the variability of UL140 ORF and different sympt... Objective To investigate the variability of human cytomegalovirus (HCMV) UL140 open reading flame (ORF) in clinical strains, and to explore the relationship between the variability of UL140 ORF and different symptoms of HC-MV infection. Methods HCMV UL140 ORF was amplified by polymerase chain reaction and sequenced selectedly in 30 clinical strains. Results UL140 ORF of all clinical strains was amplified successfully. Compared with that of Toledo strain, the nucleotide and amino acid sequence identities among all strains were 96.5% -100.0% and 95.2% -100. 0%, respectively. All of the nucleotide changes were substitutions. The post-translational modification sites were conserved. The result of phylogenetic tree showed that the strains did not cluster according to different clinical symptoms. Conclusion HCMV UL140 ORF in clinical strains is highly conserved, which may play an important role in HC-MV infection. 展开更多
关键词 human cytomegalovirus UL140 CONSERVATION
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HIGH VARIABILITY OF HUMAN CYTOMEGALOVIRUS UL150 OPEN READING FRAME IN LOW-PASSAGED CLINICAL ISOLATES 被引量:1
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作者 Yao-hua Ji Zheng-rong Sun Qiang Ruan Rong He Ying Qi Yan-ping Ma Yu-jing Huang 《Chinese Medical Sciences Journal》 CAS CSCD 2006年第2期69-74,共6页
Objective To investigate the polymorphism of human cytomegalovirus (HCMV) UL150 open reading frame (ORF) in low-passaged clinical isolates, and to study the relationship between the polymorphism and different pathogen... Objective To investigate the polymorphism of human cytomegalovirus (HCMV) UL150 open reading frame (ORF) in low-passaged clinical isolates, and to study the relationship between the polymorphism and different pathogenesis of congenital HCMV infection. Methods PCR was performed to amplify the entire HCMV UL150 ORF region of 29 clinical isolates, which had been proven containing detectable HCMV-DNA using fluorescence quantitative PCR. PCR amplification products were sequenced directly, and the data were analyzed. Results Totally 25 among 29 isolates were amplified, and 18 isolates were sequenced successfully. HCMV UL150 ORF sequences derived from congenitally infected infants were high variability. The UL150 ORF in all 18 clinical isolates shifted backward by 8 nucleotides leading to frame-shift, and contained a single nucleotide deletion at nucleotide position 226 compared with that of Toledo strain. The nucleotide diversity was 0.1% to 6.8% and the amino acid diversity was 0.2% to 19.2% related to Toledo strain. However, the nucleotide diversity was 0.1% to 6.4% and amino acid diversity was 0.2% to 8.3% by compared with Merlin strain. Compared with Toledo, 4 new cysteine residues and 13 additional posttranslational modification sites were observed in UL150 putative proteins of clinical isolates. Moreover, the UL150 putative protein contained an additional transmembrane helix at position of 4-17 amino acid related to Toledo. Conclusion HCMV UL150 ORF and deduced amino acid sequences of clinical strains are hypervariability. No obvious linkage between the polymorphism and different pathogenesis of congenital HCMV infection is found. 展开更多
关键词 human cytomegalovirus GENE UL150
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Evaluation on Clinical Application of Three Testing Methods for Human Cytomegalovirus Infection in Pregnancy 被引量:1
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作者 曾万江 闻良珍 +1 位作者 陈素华 凌霞珍 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2003年第2期192-194,共3页
The value of ELISA, N-PCR and RT-PCR in clinical practice for pregnant women with HCMV infection was investigated. 5581 pregnant women were screened by ELISA. Among them, 100 cases were positive for IgM (group 1). 69 ... The value of ELISA, N-PCR and RT-PCR in clinical practice for pregnant women with HCMV infection was investigated. 5581 pregnant women were screened by ELISA. Among them, 100 cases were positive for IgM (group 1). 69 for both IgM and serous DNA (group 2) and 69 for both IgM and mRNA (group 3). The infectious status, maternal-fetal transmission and pregnancy outcome were monitored. It was demonstrated that the accordance rate of group 3 and group 2 with group 1 was 56. 25 % and 43. 75 % , respectively. The maternal-fetal transmission rate in the group 1, 2 and 3 was 19. 00 % , 40. 58 % and 46. 15 %, respectively, with a significant difference found between group 2, 3 and group 1 (P<0. 01). Incidence of spontaneous abortion, fetal death, fetal abnormality and neonatal death in group 1, 2 and 3 was 10. 00 %, 15. 94 % and 30. 77 %, respectively, and that of group 3, 2 was 4 and 2 times as much as that of group 1, respectively (OR = 4. 00, P<0. 001; OR=2. 343, P<005, respectively). It was concluded that HCMV-IgM(+) can only be considered as an screening indicator for pregnant women with HCMV infection, while IgM(+) combined with serous DNA( + ) or mRNA( + ) indicates active infection and has a high incidence of maternal-fetal transmission and abnormal pregnancy outcome. 展开更多
关键词 human cytomegalovirus enzyme linked immunosorbent assay polymerase chain reaction : pregnancvaction PREGNANCY
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Association of TRIM22 with the type 1 interferon response during primary human cytomegalovirus infection in THP-1 macrophages 被引量:1
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作者 WEI LI HUIHUI GAO +2 位作者 RAN TAO LIFANG LIU SHIQIANG SHANG 《BIOCELL》 SCIE 2019年第4期285-291,共7页
As a response factor of interferon,tripartite motif(TRIM)22 was reported to exert antiviral activity against viruses.In this study,THP-1 macrophages were infected with human cytomegalovirus(HCMV)to establish the HCMV ... As a response factor of interferon,tripartite motif(TRIM)22 was reported to exert antiviral activity against viruses.In this study,THP-1 macrophages were infected with human cytomegalovirus(HCMV)to establish the HCMV lytic infection model.The mRNA levels of interleukin-6(IL-6),tumor necrosis factor-alpha(TNF-α)and interferonbeta(IFN-β)were significantly up-regulated in THP-1 macrophages at different infection time and titers.Moreover,for the first time,upregulation of TRIM22 expression was found during HCMV infection at both mRNA and protein levels in THP-1 macrophages.Furthermore,IFN-β could induce TRIM22 expression in THP-1 macrophages or HCMV infected THP-1 macrophages.Depletion of TRIM22 increased replication activity of HCMV with increasing of HCMV titers and HCMV proteins.In conclusion,it is the first report that HCMV can induce TRIM22 activation through IFN-β signaling and TRIM22 can suppress replication of HCMV in THP-1 macrophages. 展开更多
关键词 human cytomegalovirus TRIM22 IFN-βReplication
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Murine brain tissues with human cytomegalovirus infection: a proteomic study
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作者 Xie Ni Yuan Jianhui +1 位作者 Cai Yinsha Liu Jianjun 《Journal of Medical Colleges of PLA(China)》 CAS 2010年第2期65-74,共10页
To establish two-dimensional electrophoresis profiles with high resolution and reproducibility from murine brain tissues by human cytomegalovirus(HCMV) infection and paired murine brain tissues and to identify the d... To establish two-dimensional electrophoresis profiles with high resolution and reproducibility from murine brain tissues by human cytomegalovirus(HCMV) infection and paired murine brain tissues and to identify the differential expression proteins. Methods: Forty Kunming mice were randomly divided into infection group (20) injected with HCMVAD169 and control group (20) injected with saline into their brain. After 30 days, the murine brain tissues by HCMV infection and paired murine brain tissues were separated by two-dimensional gel electrophoresis(2-DE), analyzed by Image Master 2D software, and identified by peptide mass fingerprint(PMF) and database searching, and make Western blotting analyses the differential expression of individual proteins. Results: Well resolved, reproducible 2-D maps of the above tissues were obtained. Some of the different proteins identified by mass spectrometry(MS) were matched in the SWISS-2D PAGE database, Western blotting analyses were further carried out to verify the differential expression of individual proteins. Conclusion: These data will be valuable for studying the diagnosis of disease at an early stage, mechanisms of pathogenic and the key to the development of anti-HCMV medicine. 展开更多
关键词 human cytomegalovirus INFECTION PROTEOMICS Differential expression
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Human Herpes Virus Type 2 ( HSV2 ), Human Cytomegalovirus (HCMV) in the Male Genital Tract and Fertilization
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作者 Courtot Anne Marie Pallier Coralie Testart Jacques 《Journal of Reproduction and Contraception》 CAS 2003年第2期67-79,共13页
The possibility of infection of the human male genital tract by human herpes virus type 2 (HSV2) or human cytomegalovirus (HCMV) is well established and their sexual transmission has been the object of many studies. M... The possibility of infection of the human male genital tract by human herpes virus type 2 (HSV2) or human cytomegalovirus (HCMV) is well established and their sexual transmission has been the object of many studies. Moreover, medically assisted procreation, which helps in numerous fertility problems, raises the question of new viral risks linked to the application of these new technologies. In this review, we shall consider current knowledge in terms of the presence of HSV 2 and HCMV in the different parts of the genital tract of immunocompetent or immunodepressed men. We shall also consider the possibility of viral transmission by the sexual act or by the various techniques used in medically assisted procreation. We shall describe studies in human beings and in animals. 展开更多
关键词 FERTILIZATION human herpes virus type 2 (HSV2) human cytomegalovirus (HCMV) male genital tract medically assisted procreation (MAP)
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HCMV Infection Depress NGF Expression in Human Glioma Cells
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作者 Hai-tao WANG Bin WANG +5 位作者 Zhi-jun LIU Zhi-qiang BAI Ling LI Dong-meng QIAN Zhi-yong YAN Xu-xia SONG 《Virologica Sinica》 SCIE CAS CSCD 2009年第3期209-214,共6页
Human cytomegalovirus (HCMV) is the most common cause of congenital infection, resulting in birth defects such as microcephaly. In this study, RT-PCR and Western Blotting were performed to quantify the regulation of... Human cytomegalovirus (HCMV) is the most common cause of congenital infection, resulting in birth defects such as microcephaly. In this study, RT-PCR and Western Blotting were performed to quantify the regulation of endogenic nerve growth factor expression in neuroglia ceils by HCMV infection. The results showed that basal, endogenous NGF expression in U251 was unchanged during early HCMV infection. NGF expression is strongly down-regulated during the latent phase of infection. These results suggest that HCMV can depress the NGF expression in U251 cells. 展开更多
关键词 human cytomegalovirus (HCMV) U251 cell Nerve growth factor (NGF)
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Cytomegalovirus glycoprotein B sequence variation in Chinese liver transplant recipients
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作者 Shu-Sen Zheng Lin Zhou +3 位作者 Jing Qian Ting Cai Jun Fan Wei-Hang Ma From the Institute of Organ Transplantation and the Department of Microbiology First Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310003, China 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2002年第1期26-29,共4页
Objective: To investigate human cytomegalovirus infec-tion and genetic variations in glycoprotein B(gB) inliver transplant recipients in south-east China.Methods:EDTA-blood samples were obtained from 21liver transplan... Objective: To investigate human cytomegalovirus infec-tion and genetic variations in glycoprotein B(gB) inliver transplant recipients in south-east China.Methods:EDTA-blood samples were obtained from 21liver transplant recipients. The semi-nested PCR wasused to amplify a region of high sequence variabilityin the gB gene of human cytomegalovirus (HCMV)followed by direct sequence analysis.Results: Out of the 21 liver transplant recipients, 5were proved HCMV positive 62 to 180 days aftertransplantation. The nucleotide and encoded aminoacid sequences were compared with published se-quences of AD169 and Towne laboratory strains.Within the region sequenced, 2 out of 5 strains pos-sessed a peptide configuration similar to that of strainAD169, while another 2 strains displayed a peptideconfiguration similar to that of strain Towne. Onestrain had amino acid substitution, which was differ-ent from those of both AD169 and Towne in thecleavage site.Conclusion: Our results provide molecular epidemio-logical data for HCMV strains circulating among trans-plant recipients in south-east China. 展开更多
关键词 human cytomegalovirus glycoprotein B liver transplantation
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Single-cell RNA-sequencing reveals a profound immune cell response in human cytomegalovirus-infected humanized mice
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作者 An Wang Xiao-Xu Zhu +6 位作者 Yuanyuan Bie Bowen Zhang Wenting Ji Jing Lou Muhan Huang Xi Zhou Yujie Ren 《Virologica Sinica》 SCIE CAS CSCD 2024年第5期782-792,共11页
Human cytomegalovirus(HCMV)is a common herpesvirus that persistently infects a large portion of the world's population.Despite the robust host immune response,HCMV is able to replicate,evade host defenses,and esta... Human cytomegalovirus(HCMV)is a common herpesvirus that persistently infects a large portion of the world's population.Despite the robust host immune response,HCMV is able to replicate,evade host defenses,and establish latency throughout the lifespan by developing multiple immunomodulatory strategies,making the studies on the interaction between HCMV infection and host response particularly important.HCMV has a strict host specificity that specifically infects humans.Therefore,most of the in vivo researches of HCMV rely on clinical samples.Fortunately,the establishment of humanized mouse models allows for convenient in-lab animal experiments involving HCMV infection.Single-cell RNA sequencing enables the study of the relationship between viral and host gene expressions at the single-cell level within host cells.In this study,we assessed the gene expression alterations of PBMCs at the single-cell level within HCMV-infected humanized mice,which sheds light onto the virus-host interactions in the context of HCMV infection of humanized mice and provides a valuable dataset for the related researches. 展开更多
关键词 human cytomegalovirus(HCMV) Single-cell RNA-Sequencing PBMC Viral infection
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应用PCR技术对孕妇HCMV感染的前瞻性研究 被引量:6
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作者 欧知硕 宋文刚 +2 位作者 殷宪敏 高丽英 高广燕 《中国优生与遗传杂志》 1999年第6期30-31,共2页
应用聚合酶链反应(PCR)技术对156 例孕妇尿中HCMV 进行检测,并追踪观察114 例新生儿脐血中HCMV 垂直传播情况。结果表明:11 例孕妇尿HCMVDNA 检测阳性,孕期感染率为7-05% ,有异常妊娠史孕妇感... 应用聚合酶链反应(PCR)技术对156 例孕妇尿中HCMV 进行检测,并追踪观察114 例新生儿脐血中HCMV 垂直传播情况。结果表明:11 例孕妇尿HCMVDNA 检测阳性,孕期感染率为7-05% ,有异常妊娠史孕妇感染率(14-71 % )较正常孕妇(4-92 %) 明显升高。9 例HCMV 阳性孕妇有4 例观察新生儿脐血HCMVDNA 阳性,占44% ,而HCMVDNA阴性孕妇无1 例新生儿脐血出现阳性结果。我们认为PCR方法是检测HCMV宫内感染的可靠而灵敏的指标,对HCMVDNA阳性的孕妇应进一步检查羊水,如羊水HCMVDNA也阳性,应终止妊娠。 展开更多
关键词 孕妇 聚合酶链反应 巨细胞病毒感染
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Onset of Coronary Heart Disease is Associated with HCMV Infection and Increased CD14^+CD16^+Monocytes in a Population of Weifang,China 被引量:10
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作者 LI Hong Zheng WANG Qin +5 位作者 ZHANG Yi Yuan WANG Jin Dong WU Hong Juan ZHANG Mo Gen LI Ji Chen LIU Zhi Jun 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2020年第8期573-582,共10页
Objective To investigate the relationship between human cytomegalovirus(HCMV)infection and peripheral blood CD14+CD16+monocytes in the pathogenesis of coronary heart disease(CHD),and to elucidate the mechanism of path... Objective To investigate the relationship between human cytomegalovirus(HCMV)infection and peripheral blood CD14+CD16+monocytes in the pathogenesis of coronary heart disease(CHD),and to elucidate the mechanism of pathogenesis in CHD by analyzing the correlation between infection,inflammation,and CHD,to provide a basis for the prevention,evaluation,and treatment of the disease.Methods In total,192 patients with CHD were divided into three groups:latent CHD,angina pectoris,and myocardial infarction.HCMV-IgM and-IgG antibodies were assessed using ELISA;CD14+CD16+monocytes were counted using a five-type automated hematology analyzer;mononuclear cells were assessed using fluorescence-activated cell sorting;and an automatic biochemical analyzer was used to measure the levels of triglyceride,cholesterol,high-and low-density lipoprotein cholesterols,lipoprotein,hs-CRp and Hcy.Results The positive rates of HCMV-IgM and-IgG were significantly higher in the CHD groups than in the control group.HCMV infection affects lipid metabolism to promote immune and inflammatory responses.Conclusion HCMV infection has a specific correlation with the occurrence and development of CHD.The expression of CD14+CD16+mononuclear cells in the CHD group was increased accordingly and correlated with acute HCMV infection.Thus,HCMV antibody as well as peripheral blood CD14+CD16+mononuclear cells can be used to monitor the occurrence and development of CHD. 展开更多
关键词 human cytomegalovirus Coronary heart disease ANTIBODY CD14+CD16+monocytes Weifang
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Expression of human cytomegalovirus components in the brain tissues of patients with Rasmussen's encephalitis 被引量:9
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作者 Yao Zhang Yisong Wang +6 位作者 Sichang Chen Shuai Chen Yuguang Guan Changqing Liu Tianfu Li Guoming Luan Jing An 《Virologica Sinica》 SCIE CAS CSCD 2017年第2期115-121,共7页
Rasmussen's encephalitis(RE) is a rare and severe progressive epileptic syndrome with unknown etiology. Infection by viruses, including human cytomegalovirus(HCMV), has been speculated to be a potential trigger fo... Rasmussen's encephalitis(RE) is a rare and severe progressive epileptic syndrome with unknown etiology. Infection by viruses, including human cytomegalovirus(HCMV), has been speculated to be a potential trigger for RE. However, no viral antigens have been detected in the brains of patients with RE; thus, a possible clinical linkage between viral infections and RE has not been firmly established. In this study, we evaluated the expression of HCMV pp65 antigen in brain sections from 26 patients with RE and 20 non-RE patients by immunohistochemistry and in situ hybridization, and assessed the associations between HCMV infection and clinical parameters.Elevated expression of HCMV pp65 protein and DNA was observed in 88.5%(23/26) and 69.2%(18/26) of RE cases, respectively. In the non-RE group, HCMV pp65 antigen was detected only in two cases(10%), both of which were negative for DNA staining. Additionally, the intensity of HCMV pp65 staining was correlated with a shorter duration of the prodromal stage, younger age of seizure onset, and more severe unilateral cortical atrophy. Elevated expression of HCMV pp65 was observed in RE brain tissue and was correlated with the clinical features of RE disease. In summary, our results suggested that HCMV infection may be involved in the occurrence and progression of RE disease. Thus, further studies are needed to determine whether early treatment with anti-HCMV antibodies could modulate the course of RE. 展开更多
关键词 Rasmussen’s encephalitis human cytomegalovirus(HCMV) PP65 EPILEPSY
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