Objective To construct green fluorescent protein (GFP)-labeled pSELECT-GFP zeohBMP2 eukaryotic expression vector.Methods The encoding fragment of hBMP2 gene was obtained from a recombinant plasmid pcDNA3.1/CT-hBMP2 by...Objective To construct green fluorescent protein (GFP)-labeled pSELECT-GFP zeohBMP2 eukaryotic expression vector.Methods The encoding fragment of hBMP2 gene was obtained from a recombinant plasmid pcDNA3.1/CT-hBMP2 by using polymerase展开更多
40 human endometrial tissues of benign diseases were diviided into 8 groups (phases),and 50 BDF1 mousy endometrial tissues were divided into 5 groups (phases).Immunohistochenical staining was performed to show the PCN...40 human endometrial tissues of benign diseases were diviided into 8 groups (phases),and 50 BDF1 mousy endometrial tissues were divided into 5 groups (phases).Immunohistochenical staining was performed to show the PCNA (proliferating cell nuclear antigen)positive cell .In human ,the results suggested that the PCNA layer(48%)of mid-proliferative hpase.The PCNA labelled index was low in superficial epithelium.But in mouse, the results suggested that the Pcna labelled index was the highest(22%)in endometrium of estrus and the labelled cells were distributed mainly in superficial epithelium (60%).The results suggested that times that times and positions of cellular proliferation in endometrial tissues of human and mouse differed greatly.展开更多
文摘Objective To construct green fluorescent protein (GFP)-labeled pSELECT-GFP zeohBMP2 eukaryotic expression vector.Methods The encoding fragment of hBMP2 gene was obtained from a recombinant plasmid pcDNA3.1/CT-hBMP2 by using polymerase
文摘40 human endometrial tissues of benign diseases were diviided into 8 groups (phases),and 50 BDF1 mousy endometrial tissues were divided into 5 groups (phases).Immunohistochenical staining was performed to show the PCNA (proliferating cell nuclear antigen)positive cell .In human ,the results suggested that the PCNA layer(48%)of mid-proliferative hpase.The PCNA labelled index was low in superficial epithelium.But in mouse, the results suggested that the Pcna labelled index was the highest(22%)in endometrium of estrus and the labelled cells were distributed mainly in superficial epithelium (60%).The results suggested that times that times and positions of cellular proliferation in endometrial tissues of human and mouse differed greatly.