The human granulocytes were isolated.Using hybridoma techniques,a hybridoma cell line (HSN) producing monoclonal antibody(McAb) against human granulocyte was obtained.The antibody belonged to IgG1 subclass.It was conf...The human granulocytes were isolated.Using hybridoma techniques,a hybridoma cell line (HSN) producing monoclonal antibody(McAb) against human granulocyte was obtained.The antibody belonged to IgG1 subclass.It was confirmed by immunohistochemical tests that HSN reacted selectively not only with human granulocytes.but also with their bone marrow precursors.Whereas human lymphocytes and red blood cells retained negative in the tests.No cross-reaction was observed with the peripheral blood cells in other animals.Its affinity constant was 5.7×10^8 L/mol,and the number of epitopes per granulocyte was 4.7×10^5.Monoclonal antibody displayed no loss of immunoreactivty after labelled with ^99mTc.展开更多
Fifteen hybridoma cell lines producing monoclonal antiboclies (McAb) against recombinant human tu-mor necrosis factor a (rhTNFa) have been established by fusing SP 2/0 cells with spleen cells from aBALB/c mouse immuni...Fifteen hybridoma cell lines producing monoclonal antiboclies (McAb) against recombinant human tu-mor necrosis factor a (rhTNFa) have been established by fusing SP 2/0 cells with spleen cells from aBALB/c mouse immunized with rhTNFa. Following J M Davis’s Works, semi-solid medium was usedfor initial cloning. Five of them were studied further. Their main chromosome- numbers range were 96 to105, all of them were IgG1 subclass. The affinities of these McAbs were estimated to be 1. 25 ×108 mol/L, 1. 12×108 mol/L, 2. 34×108 mol/L, 8. 55 × 107 mol/L, 1. 04×108 mol/L, respectively.Two groups of mice challenging with E Coli (107 organisms), one group treated with 2mg/kg anti-TNF monoclonal antibody, the other did not. There was a higher survival rate in treated group, the serumTNF level was significantly lower too, and the untreated mice had severe pathologic changes in vlscera.展开更多
基金Supported by National Natural Scence Foundation of China under Contract No.39370218International Atomic Energy Agency under Research Contract No.9466/RO
文摘The human granulocytes were isolated.Using hybridoma techniques,a hybridoma cell line (HSN) producing monoclonal antibody(McAb) against human granulocyte was obtained.The antibody belonged to IgG1 subclass.It was confirmed by immunohistochemical tests that HSN reacted selectively not only with human granulocytes.but also with their bone marrow precursors.Whereas human lymphocytes and red blood cells retained negative in the tests.No cross-reaction was observed with the peripheral blood cells in other animals.Its affinity constant was 5.7×10^8 L/mol,and the number of epitopes per granulocyte was 4.7×10^5.Monoclonal antibody displayed no loss of immunoreactivty after labelled with ^99mTc.
文摘Fifteen hybridoma cell lines producing monoclonal antiboclies (McAb) against recombinant human tu-mor necrosis factor a (rhTNFa) have been established by fusing SP 2/0 cells with spleen cells from aBALB/c mouse immunized with rhTNFa. Following J M Davis’s Works, semi-solid medium was usedfor initial cloning. Five of them were studied further. Their main chromosome- numbers range were 96 to105, all of them were IgG1 subclass. The affinities of these McAbs were estimated to be 1. 25 ×108 mol/L, 1. 12×108 mol/L, 2. 34×108 mol/L, 8. 55 × 107 mol/L, 1. 04×108 mol/L, respectively.Two groups of mice challenging with E Coli (107 organisms), one group treated with 2mg/kg anti-TNF monoclonal antibody, the other did not. There was a higher survival rate in treated group, the serumTNF level was significantly lower too, and the untreated mice had severe pathologic changes in vlscera.
