The Social Construction of City： Taipei in Human Condition

This paper tries to discuss everyday life and its relation to urban experiences in different aspects of Taipei City in Human Condition, performed by Greenray Theatre in Taiwan since 2001. The serial play, including six parts,1 displays the life of ordinary people, primarily the middle and labor classes. Historically, Human Condition tells about Taiwan Residents＇ life from the period of Japanese occupation in Taiwan to the present day. The public＇s everyday life and their cultural activities are delicately represented on the stage. Human Condition especially focuses on people living in Taipei city, the capital and most populated place of the country. In different historical periods and political atmospheres, the characters＇ economic and social activities, nostalgic feelings about the past, passions for love and friendship, and awakening of fighting for humanity and freedom construct their social positions as well as establish different aspects of Taipei. We can see the transformation of the city by means of people＇s social relationships and economic activities. Drawing on Henri Lefebvre＇s concept of everyday life as rhythmic movement and spatial practice in the process of urbanization, this paper aims at studying the characters＇ social practice that defines their position and formation of identity within place. Human Condition unravels the characters＇ connection and disconnection from place in each period of Taiwan＇s historical and political conditions. The life in Taipei differs from each historical, economic, and political system that defines people＇s attitudes and perspectives toward this land. The characters within every historical background and political system tell us what Taipei represents and symbolizes to people. The transition of Taipei produces impacts on people that endow them with different personalities and perspectives toward life. As globalization invades into this city, the definition of human in city life has to be reconsidered. As a place of encounter, accumulation of cultural hybridity in Taipei formulates multiple dispositions and social structures. By analyzing the characters represented in every historical period of Taipei, this article interrogates to what extent of ＂in-place＂ or ＂out-of-place＂ of the characters is interpreted in this space and, in the progress of urbanized development of Taipei, what the value is to affect and connect people＇s relationships.

Enhancing Erectile Function and Alleviating Andropause Symptoms: Clinical Efficacy of a Human Stem Cell Conditioned Medium Cream

Erectile dysfunction (ED) is increasingly prevalent in Japan, exceeding 30%, and increasing with age. Unhealthy lifestyle habits, obesity, insufficient exercise, and smoking have been implicated in its pathogenesis, along with endothelial dysfunction of the corpora cavernosa and impaired blood flow to the penis considered underlying factors. However, the current treatments are limited to Phosphodiesterase-5 (PDE5) inhibitors. ED is the primary symptom of andropathy. This study reports the clinical efficacy of human stem cell-conditioned medium cream for ED treatment. Ten men without underlying diseases suspected of andropause with ED (mean age 43.2 ± 4.4 y, Hb 15.2 ± 0.6 gm/dL, AST/ALT 30.2/37.9 ± 12.4/14.0, eGFR 82.7 ± 12.4 mL/min/1.73 m2) were targeted. The cream was applied twice daily to the genital and scrotal areas. The erectile hardness score (EHS), International Index of Erectile Function-5 (IIEF-5), and Aging Male Symptoms (AMS) scale were used to evaluate the participants before and 30 days after use, and the results were compared using paired t-tests. The post-use qualitative opinions were collected through interviews. Significant improvements were observed compared to baseline in the IIEF-5 (11.8 ± 4.6→17.2 ± 5.1, P < 0.001), and AMS (46.3 ± 6.7→37.6 ± 5.3, P < 0.001) scores post cream use. EHS did not show a statistically significant difference, but a trend towards improvement was observed. Qualitative feedback included increased morning erection, improved maintenance of erection during intercourse, and reduced post work fatigue. Human stem cell-conditioned medium contains endothelial growth factors that potentially contribute to the improvement of ED and andropause by enhancing corporal endothelial function. Future studies should include control groups to further investigate the efficacy of these treatments.

In vitro differentiation of human adipose-derived adult stromal cells into neuron-like cells in hippocampal astrocyte conditioned medium

BACKGROUND: At present, researches on differentiating from human adipose-derived adult stromal cells (hADASC) to neuron-like cells are focus on inducing by artificial-synthetic compound solution; however, hippocampal astrocyte conditioned medium (HCAM) can induce in vitro differentiation from hADASC to neuron-like cells is still unclear. OBJECTIVE: To observe whether HCAM can induce in vitro differentiation from hADASC to neuron-like cells. DESIGN: Randomized control study. SETTING: Department of Neurology, Taixing People's Hospital; Central Laboratory, North China Coal Medical College. MATERIALS: Donor of adipose tissue was donated by female volunteers suffering from caesarean section in the department of obstetrics & gynecology in our hospital and aged 20-35 years. Adipose tissue was collected from subcutaneous tissue of abdomen during the operation. In addition, 8 male newborn Wistar rats within 24 hours with average body mass of 20 g were provided by Animal Institute of Chinese Academy of Medical Sciences. Rabbit-anti-human Nestin polyclonal antibody, rabbit-anti-human glial fibriliary acidic protein (GFAP) polyclonal antibody, rabbit-anti-human neuro-specific enolase polyclonal antibody and mouse-anti-human microtubal associated protein 2 (MAP-2) polyclonal antibody were provided by Wuhan Boster Company. METHODS: The experiment was carried out in the Central Laboratory of North China Coal Medical College from October 2004 to June 2005. hADASC was cultured with HCAM and its growth and morphological changes were observed under inverted phase contrast microscope. Immunocytochemistry, immunofluorescence and Western blotting were used to evaluate the expressions of Nestin, which was a specific sign of nerve precursor, neuro-specific enolase and MAP-2, which was a specific sign of nerve cell, and GFAP, which was a specific sign of neuroglial cells. MAIN OUTCOME MEASURES: Nestin, which was a specific sign of nerve precursor, neuro-specific enolase and MAP-2, which was a specific sign of nerve cell, and GFAP, which was a specific sign of neuroglial cells. RESULTS: On the 3rd day of culture, partial hADASC started deformation from slender shuttle-shape cells to neuron-like cells. It suggested that cells stretched out apophysis, which were mainly double-pole or multiple-pole cells. Five days later, immunohistochemical detection suggested that expression of Nestin (10.5±0.037) was found out in cells; meanwhile, expressions of GFAP (38.4±0.052) and neuro-specific enolase (NSE) (15.7±0.023) were also found out in cells; however, expression of MAP-2 was not observed. Western blot indicated that, 5 days after effect of HCAM, Nestin was found out in hADASC; meanwhile, expressions of GFAP and neuro-specific enolase were also found out; however, expression of MAP-2 was not observed. CONCLUSION: HCAM can induce the differentiation from hADASC to neuron-like cells in vitro.

CONDITIONED MEDIUM OF HUMAN NASOPHARYNGEAL CARCINOMA EPITHELIOID CELL LINE CNE_(1) CONTAINED THE ACTIVITIES OF TRANSFORMED GROWTH-INHIBITING FACTORS

The hormone defined serum free conditioned medium (SFCM) of human nasopharyngeal carcinoma epithelioid cell line (CNE1) was assayed by both the 3H-thymidine incorporation test and the soft agar test. It was found that the SFCM stimulated the growth of long-term serum-free cultured CNE4 cells in ac-cordence with the fact that the growth rate of long-term serum-free cultured CNE1 cells was directly proportional to the plating density. Alternatively 5% SFCM inhibited the growth of short-term serum-free cultured CNE4 cells by 51% in which the indicator cell remained the responsiveness state of growing in the serum-supplemented medium to the effector of interest. Furthermore, SFCM resulted in the inhibition of anchorage-independent growth of CNE4 cells and A431 cells. Also in soft agar test. SFCM reduced the colony formation of NRK(?),9F cells in the presence of EGF or EGF plus TGF-β. These finding suggested that CNE4 secreted autocrine growth stimulating factor(s) and growth inhibiting factor(s) in the serum-free medium, the latter strongly reverse malignant phenotypes of CNE4 and A431 cells in serum-supplemented surrounding.

Law Professor: Changes "Substantial" in China's Human Rights Conditions

"Substantial" changes have taken place in China’s human rights conditions, according to Professor Xu Xianming, president of the China University of Law and Political Sciences. As a member of the Law Committee of the Standing Committee of the National People’s Congress, China’s highest legislature, he is known for his contributions to improvements in the country’s legal system. In an interview with Human Rights magazine, Professor Xu Xianming listed what he chose to call the "five most important changes" in China’s human rights conditions:

A Witness's Account of Changes in Tibet's Human Rights Conditions

After riots that occurred on March 14, 2008 in Lhasa, Tibet, the China Central Television Station broadcast a documentary film titled Tibet＇s Past shot years ago by the Central Newsreel ＆ Documentary Film Studio about the miserable life of serfs under Tibet＇s feudal serf system before a democratic reform was carried out in the region. The documentary stunned many people. It helps people see through the reactionary and corrupt nature of the Dalai clique.

Zhao Qizheng: I'm Fully Confident of China's Human Rights Conditions in Future

Following is a summary of an interview given by Zhao Qizheng, director-general of the State Council Information Office (SCIO), to mark the launching of this magazine. In the interview, Zhao elaborated on the current human rights conditions in China and their developments, why there are differences between the East and West regarding human rights, and China's stand on the issue.

Effects of Anthropogenic Disturbance on Sediment Organic Carbon Mineralization Under Different Water Conditions in Coastal Wetland of a Subtropical Estuary

The changes in soil organic carbon(C) mineralization as affected by anthropogenic disturbance directly determine the role of soils as C source or sink in the global C budget. The objectives of this study were to investigate the effects of anthropogenic disturbance(aquaculture pond, pollutant discharge and agricultural activity) on soil organic C mineralization under different water conditions in the Minjiang River estuary wetland, Southeast China. The results showed that the organic C mineralization in the wetland soils was significantly affected by human disturbance and water conditions(P < 0.001), and the interaction between human disturbance activities and water conditions was also significant(P < 0.01). The C mineralization rate and the cumulative mineralized carbon dioxide-carbon(CO_2-C)(at the 49th day) ranked from highest to lowest as follows: Phragmites australis wetland soil > aquaculture pond sediment > soil near the discharge outlet > rice paddy soil. This indicated that human disturbance inhibited the mineralization of C in soils of the Minjiang River estuary wetland, and the inhibition increased with the intensity of human disturbance. The data for cumulative mineralized CO_2-C showed a good fit(R^2 > 0.91) to the first-order kinetic model C_t = C_0(1 – exp(–kt)). The kinetic parameters C_0, k and C_0 k were significantly affected by human disturbance and water conditions. In addition, the total amount of mineralized C(in 49 d) was positively related to C_0, C_0 k and electrical conductivity of soils. These findings indicated that anthropogenic disturbance suppressed the organic C mineralization potential in subtropical coastal wetland soils, and changes of water pattern as affected by human activities in the future would have a strong influence on C cycling in the subtropical estuarine wetlands.

欧盟EBA普惠制与柬埔寨人权发展效应

EBA普惠制是欧盟用来推动贸易伙伴国遵守核心人权和劳工权利的一个政策工具。柬埔寨成为检验欧盟EBA普惠制的人权条件适用的典型个案。欧盟EBA普惠制及其人权条件在柬埔寨的适用形成了"贸易-人权"非同步发展效应:贸易的正向发展效应与人权规范的反向发展效应共存。面对EBA人权条件的失效,欧盟启动针对柬埔寨的贸易特惠撤回程序,使得柬埔寨的人权发展状况有所改善,但仍未实现"贸易-人权"的同步正向发展效应。对此,本文基于外部激励模式,从条件的确定性、奖励的规模、威胁和承诺的可信性、采纳成本的规模等四个方面解释EBA人权条件在柬埔寨适用的有效性问题。

关于村级卫生人力资源配置运行中政策制约性分析——北京市M县村级卫生人力资源实地观察个案研究

在对北京市M县村级卫生人力资源进行实地观察的基础上,重点就村级卫生人力资源配置实际运行中满意度偏低、职业素质及后继者问题、相关卫生政策到村一级后有待具体实施等方面进行了政策制约性分析,并提出相关政策建议,即在农村卫生政策制定与执行方面,我国政府卫生部门的重点应当在于提高村级卫生人力资源对医疗卫生服务的利用能力,并在此制度基础上加强对农村卫生政策监管,使好的政策落到实处。

HRE和hTERT修饰条件复制型腺病毒携带Egr-1介导的Smac对人食管癌细胞Eca109周期和凋亡的影响

目的探讨以HRE和hTERT修饰条件复制型腺病毒携带Egr-1介导的Smac(CARd.pE-Smac)对人食管癌细胞Eca109周期和凋亡的影响。方法利用氯化钴进行化学乏氧,病毒感染滴度为5MOI[Multiplicity of infection(virus/cell)]感染人食管癌Eca109细胞24h,并进行2Gy照射,12h后分别利用Western blotting检测Smac蛋白的表达,PI染色和AnnexinⅤ-FITC双染,流式细胞术检测细胞周期和细胞凋亡变化。实验分为control、CARd.pESmac、hypoxia、2Gy、H+CARd.pE-Smac、CARd.pE-Smac+2Gy和H+CARd.pE-Smac+2Gy组。结果 CARd.pE-Smac感染常氧和乏氧的Eca109细胞后均未见Smac蛋白表达增加,而2Gy照射后,常氧、感染CARd.pE-Smac和乏氧再感染CARd.pE-Smac均能使Smac蛋白表达增加,尤其以三者联用后Smac表达增加最大;感染CARd.pESmac未对细胞周期有明显改变,而乏氧、2Gy和感染CARd.pE-Smac任二者(乏氧与2Gy除外)或者三者联用均能显著增加S期和G2/M期细胞百分比增加(P<0.05,P<0.01),尤其以三者联用作用更强;而且,对于细胞凋亡的诱导作用与周期进程变化的规律相类似。结论 HRE和hTERT修饰条件复制型腺病毒携带Egr-1介导的Smac在人食管癌细胞Eca109中显著过表达,且能够导致细胞发生S期延迟和G2/M期阻滞,并诱导细胞发生凋亡。

条件增殖型腺病毒PSA—ZD55-hTERT对人前列腺癌细胞移植瘤的抑制作用

目的探讨条件增殖型腺病毒PSA—ZD55-hTERT对人前列腺癌LNCaP细胞移植瘤端粒酶逆转录酶（hTERT）基因表达和肿瘤生长的抑制作用。方法建立人前列腺癌LNCaP细胞裸鼠移植瘤模型，瘤体内注射PSA—ZD55-hTERT，定期测量肿瘤体积，免疫荧光检测移植瘤组织中E1A及hTERT表达，原位末端标记法（TUNEL）检测肿瘤细胞凋亡。结果PSA—ZD55-hTERT能有效沉默hTERT基因，PSA—ZD55-hTERT组hTERT表达水平显著低于Ad—hTERT组、Ad—EGFP组及PBS组，差异显著；Ad—hTERT组、Ad—EGFP组及PBS组细胞凋亡阳性率分别为（49．7±5．1）％、（28．8±4．0）％和（2．8±2．4）％，而PSA—ZD55-hTERT细胞凋亡阳性率为（73．5±6．4）％，表明PSA—ZD55-hTERT能有效诱导肿瘤细胞凋亡；PSA—ZD55-hTERT接种7周后肿瘤体积为（131．5±28．2）mm^3，明显低于Ad—hTERT组[（469．2±74．3）mm^3]、Ad—EGFP组[（583．0±98．5）mm^3]和PBS组[（1547．5±196．4）mm^3]。结论PSA—ZD55-hTERT对裸鼠人前列腺癌LNCaP细胞移植瘤有显著的治疗效果，为治疗前列腺癌提供新的平台。

瞬时受体电位离子通道3在糖尿病肾病小鼠的表达及意义

目的:研究瞬时受体电位离子通道3(TRPM3)在2型糖尿病肾病模型db/db小鼠肾组织的表达,以及高糖对人肾小管上皮细胞(HK2)和小鼠足细胞(MPC)TRPM3表达的影响。方法:(1)将20只雄性5周龄db/db小鼠随机分为两组,各10只。同窝出生的db/m小鼠作为正常对照组(n=20)。待db/db小鼠生长至10周龄和18周龄后取血、尿标本测定相关生化指标,留取肾组织。利用Western blot、实时荧光定量PCR(qRT-PCR)和免疫组织化学方法,检测正常对照组(db/m小鼠)及不同周龄(10周、18周)db/db小鼠肾组织中TRPM3的表达水平及分布情况。(2)体外培养HK2和条件永生化MPC,分别按以下处理分组:对照组(D-葡萄糖5.6 mmol/L);甘露醇组(D-葡萄糖5.6 mmol/L+甘露醇24.4 mmol/L);高糖组(D-葡萄糖30 mmol/L),培养24h、48h、72h收获细胞,用Western blot和qRT-PCR检测细胞中TRPM3的表达。结果:(1)与db/m小鼠相比,db/db小鼠肾组织肾小管和肾小球中TRPM3的表达增多,差异有统计学意义(P<0.05);(2)与正常对照组相比,高糖组HK2和MPC中TRPM3的表达均增多,差异有统计学意义(P<0.05);结论:TRPM3可表达于糖尿病肾病小鼠肾脏组织的肾小管和肾小球中,且高血糖可诱导TRPM3在HK2细胞和MPC细胞的高表达;TRPM3可能参与了糖尿病肾病的发生发展。

基于热舒适度的节能型空调控制算法

为了提高人体热舒适度、节约能源,研究基于热舒适度的节能型空调控制算法.以热舒适度算法为系统核心,通过算法得到PMV(predicted-mean-vote)值,根据PMV值给出自适应调节的控制,实现系统需要的自适应热舒适度的目标要求,调整算法参数以达到最佳效果.

The effects of plyometric jump training on physical fitness attributes in basketball players:A meta-analysis`

Background:There is a growing body of experimental evidence examining the effects of plyometric jump training(PJT)on physical fitness attributes in basketball players;however,this evidence has not yet been comprehensively and systematically aggregated.Therefore,our objective was to meta-analyze the effects of PJT on physical fitness attributes in basketball players,in comparison to a control condition.Methods:A systematic literature search was conducted in the databases PubMed,Web of Science,and Scopus,up to July 2020.Peer-reviewed controlled trials with baseline and follow-up measurements investigating the effects of PJT on physical fitness attributes(muscle power,i.e.,jumping performance,linear sprint speed,change-of-direction speed,balance,and muscle strength)in basketball players,with no restrictions on their playing level,sex,or age.Hedge’s g effect sizes(ES)were calculated for physical fitness variables.Using a random-effects model,potential sources of heterogeneity were selected,including subgroup analyses(age,sex,body mass,and height)and single training factor analysis(program duration,training frequency,and total number of training sessions).Computation of metaregression was also performed.Results:Thirty-two studies were included,involving 818 total basketball players.Significant(p<0.05)small-to-large effects of PJT were evident on vertical jump power(ES=0.45),countermovement jump height with(ES=1.24)and without arm swing(ES=0.88),squat jump height(ES=0.80),drop jump height(ES=0.53),horizontal jump distance(ES=0.65),linear sprint time across distances≤10 m(ES=1.67)and>10 m(ES=0.92),change-of-direction performance time across distances≤40 m(ES=1.15)and>40 m(ES=1.02),dynamic(ES=1.16)and static balance(ES=1.48),and maximal strength(ES=0.57).The meta-regression revealed that training duration,training frequency,and total number of sessions completed did not predict the effects of PJT on physical fitness attributes.Subgroup analysis indicated greater improvements in older compared to younger players in horizontal jump distance(>17.15 years,ES=2.11;≤17.15 years,ES=0.10;p<0.001),linear sprint time>10 m(>16.3 years,ES=1.83;≤16.3 years,ES=0.36;p=0.010),and change-of-direction performance time≤40 m(>16.3 years,ES=1.65;≤16.3 years,ES=0.75;p=0.005).Greater increases in horizontal jump distance were apparent with>2 compared with≤2 weekly PJT sessions(ES=2.12 and ES=0.39,respectively;p<0.001).Conclusion:Data from 32 studies(28 of which demonstrate moderate-to-high methodological quality)indicate PJT improves muscle power,linear sprint speed,change-of-direction speed,balance,and muscle strength in basketball players independent of sex,age,or PJT program variables.However,the beneficial effects of PJT as measured by horizontal jump distance,linear sprint time>10 m,and change-of-direction performance time≤40 m,appear to be more evident among older basketball players.

基于人性化设计的移动空调造型设计研究

移动空调是近几年来兴起的新产品,由于本身安装便捷等特点,受到国内外客户的欢迎。经研究发现,该产品在造型设计方面仍有不足,文中从人性化的角度,针对消费者提出的问题进行了实践探索,对移动空调遥控器收纳设计、移动空调插头的绕柱设计、移动空调的风道口连接装置三个方面进行了外观造型的创新设计,将人性化设计思想渗透到细节设计中,为消费者提供更便捷、贴心的产品服务。

空调导流板角度对室内空气温度的影响

为了研究空调导流板角度对室内空气温度的影响,利用CFD软件建立了带进风口(即空调出风口)、回风口的某房间空气流动计算模型。分析了空调导流板分别为向上、0°和向下时对室内温度、风速分布的影响,以及室内人体最佳舒适度区域的大小。研究结果表明,空调导流板在0°时室内温度的均匀性最好,人体最佳舒适度区域最大。

论IMF贷款条件性与人权的国际保护

IMF贷款条件性会对成员人权实现产生积极和消极影响,特别是IMF经济援助合作框架制定等内容会涉及经济、社会、文化权利的实现。IMF原则上不愿意干涉成员人权问题,但其推动"善治"、"平等"等实践已表明其"被迫"参与了人权保护行动。为此,IMF需要对《国际货币基金组织协定》进行适当扩大解释,处理好权限范围与人权保护义务之间的关系。当IMF贷款条件性可能对成员国国民的经济权利实现产生实际影响时,IMF需要与成员进行协调,使贷款条件性设置尽可能地限于宏观经济领域范畴之内。

不同体量的汽车仪表板工程可行性

论文主要对汽车中不同体量仪表板的工程可行性进行研究,从仪表板体量的发展趋势、决定传统仪表板体量的工程约束及如何打破和重塑满足不同体量的工程约束等方面进行论述。总结仪表板体量与工程约束之间的内在关系,并梳理出如何分析找到影响仪表板体量的决定因素,并重塑工程可行方案的思路,从而实现不同体量的工程可行性。

心肌细胞损伤条件诱导液诱导人骨髓间充质干细胞的分化

目的明确SD乳鼠心肌细胞（CMs）氧化损伤性条件诱导液是否可诱导人骨髓间充质干细胞（hMSCs）向心肌样细胞分化。方法体外原代分离培养hMSCs及SD乳鼠原代CMs。搏动的CMs用过氧化氢进行损伤，将培养氧化损伤CMs的培养基制备成条件诱导液，对传代hMSCs培养诱导4周。倒置显微镜观察细胞形态，并进行CMs特异性标志物肌钙蛋白I（cTnI）及结蛋白的免疫细胞化学染色检测。结果hMSCs经CMs氧化损伤性条件诱导液诱导培养后，其形态变为宽大，诱导后细胞表达cTnI及结蛋白。结论CMs氧化损伤性条件诱导液能诱导hMSCs向心肌样细胞分化。