SGDWSDIGGR(S-10-R)is a kind of antioxidant peptide derived from broken rice.In the present study,the effects of S-10-R against H_(2)O_(2)-induced cell damage and premature senescence in 2BS cells were determined.The r...SGDWSDIGGR(S-10-R)is a kind of antioxidant peptide derived from broken rice.In the present study,the effects of S-10-R against H_(2)O_(2)-induced cell damage and premature senescence in 2BS cells were determined.The results indicated that S-10-R could improve cell viability and mitochondrial membrane potential at least 1.06 and 1.12 folds compared with senescence cells.Moreover,S-10-R inhibitedβ-galactosidase,and DNA damage alleviates the effect on 2BS senescence cells due to scavenging ROS.The apoptosis rate can be reduced by 80.58%.Furthermore,S-10-R could inhibit PI3K and Akt phosphorylation,downregulate Bax and caspases-3 expression,and inhibit premature senescence through activating PI3K/Akt related intrinsic apoptotic signaling pathways and suppressing JNK/Bcl-2 related mitochondria-dependent apoptosis.The present study confirmed the feasibility of antioxidants as anti-aging drugs,as well as provided a substantial basis for future application of S-10-R as a functional ingredient against oxidative stress-induced premature aging.展开更多
基金We appreciate the financial support from the Major Science and technology Program of Heilongjiang(2020ZX08B02)National Natural Science Foundation of China(32072258)the National Key Research and Development Program of China(2021YFD2100902-3),Central financial support for the development of local colleges and Universities.
文摘SGDWSDIGGR(S-10-R)is a kind of antioxidant peptide derived from broken rice.In the present study,the effects of S-10-R against H_(2)O_(2)-induced cell damage and premature senescence in 2BS cells were determined.The results indicated that S-10-R could improve cell viability and mitochondrial membrane potential at least 1.06 and 1.12 folds compared with senescence cells.Moreover,S-10-R inhibitedβ-galactosidase,and DNA damage alleviates the effect on 2BS senescence cells due to scavenging ROS.The apoptosis rate can be reduced by 80.58%.Furthermore,S-10-R could inhibit PI3K and Akt phosphorylation,downregulate Bax and caspases-3 expression,and inhibit premature senescence through activating PI3K/Akt related intrinsic apoptotic signaling pathways and suppressing JNK/Bcl-2 related mitochondria-dependent apoptosis.The present study confirmed the feasibility of antioxidants as anti-aging drugs,as well as provided a substantial basis for future application of S-10-R as a functional ingredient against oxidative stress-induced premature aging.
