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Expression of hepatitis B virus genes in early embryonic cells originated from hamster ova and human spermatozoa transfected with the complete viral genome 被引量:63
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作者 Bahy Ahmed Ali Tian-Hua Huang +1 位作者 Halima-Hassan Salem Qing-Dong Xie 《Asian Journal of Andrology》 SCIE CAS CSCD 2006年第3期273-279,共7页
Aim: To detect the expression of hepatitis B virus (HBV) genes (HB S and C genes) in early embryonic cells after introducing motile human sperm carrying HBV DNA into zona-free hamster oocytes via the in vitro fer... Aim: To detect the expression of hepatitis B virus (HBV) genes (HB S and C genes) in early embryonic cells after introducing motile human sperm carrying HBV DNA into zona-free hamster oocytes via the in vitro fertilization (IVF) technique. Methods: Human sperm-mediated HBV genes were delivered into zona-free hamster oocytes by the IVF method. Polymerase chain reaction (PCR) was used to detect HB S and pre-Core/Core (pre-C/C) coding genes both in one- and two-cell embryos. Reverse transcription-PCR (RT-PCR) analysis was used to study the expression of the two genes. Fluorescence in situ hybridization (FISH) analysis using the full-length HBV DNA as the hybridization probe was performed to confirm the integration of viral DNA in the host embryonic genome. Results: Both HB S and pre-C/C coding genes are present and transcribed in one- and two-cell embryos originated from hamster ova IVF with human spermatozoa carrying HBV DNA sequences. Conclusion: Sperm-mediated HBV genes are able to replicate and express themselves in early embryonic cells. These results provide direct evidence that HBV DNA could transmit vertically to the next generation via the male germ line. 展开更多
关键词 hepatitis B virus gene expression hamster ovary human spermatozoa in vitro fertilization
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Effects of 13 T Static Magnetic Fields (SMF) in the Cell Cycle Distribution and Cell Viability in Immortalized Hamster Cells and Human Primary Fibroblasts Cells 被引量:2
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作者 赵国平 陈少鹏 +8 位作者 赵烨 朱玲燕 黄佩 鲍凌志 王军 王磊 吴李君 吴跃进 许安 《Plasma Science and Technology》 SCIE EI CAS CSCD 2010年第1期123-128,共6页
Magnetic resonance image (MRI) systems with a much higher magnetic flux density were developed and applied for potential use in medical diagnostic. Recently, much attention has been paid to the biological effects of... Magnetic resonance image (MRI) systems with a much higher magnetic flux density were developed and applied for potential use in medical diagnostic. Recently, much attention has been paid to the biological effects of static, strong magnetic fields (SMF). With the 13 T SMF facility in the Institute of Plasma Physics, Chinese Academy of Sciences, the present study focused on the cellular effects of the SMF with 13 T on the cell viability and the cell cycle distribution in immortalized hamster cells, such as human-hamster hybrid (AL) cells, Chinese hamster ovary (CHO) cells, DNA double-strand break repair deficient mutant (XRS-5) cells, and human primary skin fibroblasts (AG1522) cells. It was found that the exposure of 13 T SMF had less effect on the colony formation in either nonsynchronized or synchronized AL cells. Moreover, as compared to non-exposed groups, there were slight differences in the cell cycle distribution no matter in either synchronized or nonsynchronized immortalized hamster ceils after exposure to 13 T SMF. However, it should be noted that the percentage of exposed AG1522 cells at G0/G1 phase was decreased by 10% as compared to the controls. Our data indicated that although 13 T SMF had minimal effects in immortalized hamster cells, the cell cycle distribution was slightly modified by SMF in human primary fibroblasts. 展开更多
关键词 static magnetic fields immortalized hamster cells human primary fibroblasts cells
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Expression of Calbindin-d28k in Human Endometrium 被引量:2
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作者 Duo XU Wei-jie ZHU 《Journal of Reproduction and Contraception》 CAS 2007年第4期247-252,共6页
Objective To investigate the expression of Calbindin-d28k (CaBP-d28k) in human endometrium. Methods Thirty-three samples of human normal endometrial tissues were divided into 6 groups: early proliferative stage (n... Objective To investigate the expression of Calbindin-d28k (CaBP-d28k) in human endometrium. Methods Thirty-three samples of human normal endometrial tissues were divided into 6 groups: early proliferative stage (n =6), mid proliferative stage (n =5), late proliferative stage (n=5), early secretory stage (n=7), mid secretory stage (n=5) and late secretory stage (n=5). The expression and change of CaBP-d28k protein and gene were determined by immunohistochemistry and reverse transcription polymerase chain reaction methods. Results In endometrial samples, the expression of CaBP-d28k protein was mainly observed in the cytoplasm of luminal and glandular epithelium. In the menstrual cycle, the level of CaBP-d28k protein in the epithelium was the lowest during the early and mid proliferative stages, and was the highest during the mid secretory stage, then decreased in the late secretory stage (P〈0.05). In the stroma, the expressed type of CaBP-d28k protein was the same as in the epithelium, but was lower than that in the epithelium(P〈0.05). The CaBP-d28k mRNA was at the lowest level in the early proliferative stage(P〈0.05), and significantly increased in the late proliferative, and early, mid secretory stages (P〈0. 05). Conclusion Both CaBP-d28k protein and gene were expressed in human endometrium, and their expression had cyclic changes. 展开更多
关键词 human endometrium CALBINDIN-D28K
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Formation of Nucleolar Channel System in Human Endometrium in vitro is Independent of Progesterone 被引量:1
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作者 Zi-nengWANG DanHUANG Pei-erZHENG 《Journal of Reproduction and Contraception》 CAS 2003年第2期81-86,共6页
Objective To determine whether formation of the nucleolar channel system (NCS) in human endometrium depends on the presence of progesteronal steroids. Materials & Methods Tissues of late proliferative endometrium ... Objective To determine whether formation of the nucleolar channel system (NCS) in human endometrium depends on the presence of progesteronal steroids. Materials & Methods Tissues of late proliferative endometrium were obtained from 5 normally cycling women of reproductive age. Half of each tissue was cultured in the DMEM medium containing diethylstilbesterol (25 μg/mL) plus medroxyprogesterone acetate (25 μg/mL) (E + P culture). As a control, the other half was cultured in the medium alone. After 100 h incubation, the tissues were assessed for the formation of NCS with transmission electron microscope.Results NCS was observed in the endometrial epithelium treated with E + P or the medium alone. Moreover, giant mitochondria and glycogen accumulation were both seen in epithelia derived from both types of cultures.Conclusion Progesterone would be not indispensable for the formation of NCS in human endometrium. Transition of proliferative endometrium to the secretory stage in vitro could occur even in the absence of both estrogen and progesterone. 展开更多
关键词 nucleolar channel system human endometrium tissue culture
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Failure of hCG/LH receptors to stimulate the transmembrane effector adenylyl cyclase in human endometrium
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作者 L. Bernardini I. Moretti-Rojas +2 位作者 M. Brush F. J. Rojas J. P. Balmaceda 《Advances in Bioscience and Biotechnology》 2013年第10期949-957,共9页
The functional significance of the endometrial hCG/ LH receptors has been related to a rapid release of prostaglandins. However, as compared to gonads and myometrium, in-endometrium mechanisms of transmembrane signall... The functional significance of the endometrial hCG/ LH receptors has been related to a rapid release of prostaglandins. However, as compared to gonads and myometrium, in-endometrium mechanisms of transmembrane signalling of the hCG/LH receptors are probably not conventional and remain unclear. Here we investigated, in vivo, the potential of hCG to interact with, and stimulate the membrane effector enzyme, adenylyl cyclase (AC), in human endometrium. Hormonal and nonhormonal activation of AC was tested in membrane fractions prepared from endometrial biopsies obtained from patients undergoing evaluation cycles for hormone replacement therapy (HRT) and controlled ovarian hyperstimulation (COH). AC activity was determined by the direct conversion of the substrate ATP into cAMP under unstimulated conditions and in the presence of the non-hormonal activators guanyl nucleotide and forskolin. Also AC activity was tested in the presence of hCG under conditions allowing maximal enzyme stimulation. Isoproterenol and prostaglandin E2 (PGE2) were included for comparison. Immunoblot analyses demonstrated the presence of hCG/LH receptors and Gsα protein and other members of the G protein family in the membrane fractions. Endometrial membranes also exhibited high levels of AC activity compared to luteal membranes used as control. Stimulation by GMP-P(NH)P alone was 196 ± 63 (n = 8) (pmol/mg/ min ± SD). Neither hCG nor isoproterenol showed stimulation of endometrial AC (210 ± 65, and 197 ± 53, respectively;n = 66 assays). But PGE2 stimulated the enzyme system significantly (264 ± 63, p < 0.05;n = 66 assays). These data show that membrane fractions from human endometrium express all the AC system components, namely, hCG/LH receptors, Gsα protein and AC;however, hCG does not stimulate the endometrial AC system. Our data indicate that, in great contrast to gonadal receptors, endometrial hCG/ LH receptors are not coupled to the transmembrane AC effector. The well known release of eicosanoids in response to hCG suggests that these receptors are functional in human endometrium but throughout a signalling system different from AC. This enzyme is certainly coupled to and directly activated by eicosanoids and other embryonic signals. 展开更多
关键词 hCG/LH RECEPTORS human endometrium Membrane Signal TRANSDUCTION Adenylyl CYCLASE
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The in vivo model of cross-species rabbit anterior eye chamber of human endometrium
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作者 吴燕婉 袁冬 +2 位作者 石心泉 邢志军 宗书东 《生殖医学杂志》 CAS 1996年第S1期52-56,共5页
Theinvivomodelofcross-speciesrabbitanterioreyechamberofhumanendometriumWuYanwan(吴燕婉),YuanDong(袁冬),ShiXinquan... Theinvivomodelofcross-speciesrabbitanterioreyechamberofhumanendometriumWuYanwan(吴燕婉),YuanDong(袁冬),ShiXinquan(石心泉),XingZhijun(... 展开更多
关键词 RABBIT The cross endometrium eye human in MODEL of ANTERIOR
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Expression of hepatitis B virus genes in early embryonic cells originated from hamster ova and human spermatozoa transfected with the complete viral genome 被引量:5
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作者 BabyAhmedAli Halima-HassanSalem 《Asian Journal of Andrology》 SCIE CAS CSCD 2006年第A03期273-279,385,共5页
Aim:To detect the expression of hepatitis B virus(HBV)genes(HB S and C genes)in early embryonic cells after introducing motile human sperm carrying HBV DNA into zona-free hamster oocytes via the in vitro fertilization... Aim:To detect the expression of hepatitis B virus(HBV)genes(HB S and C genes)in early embryonic cells after introducing motile human sperm carrying HBV DNA into zona-free hamster oocytes via the in vitro fertilization(IVF) technique.Methods:Human sperm-mediated HBV genes were delivered into zona-free hamster oocytes by the IVF method.Polymerase chain reaction(PCR)was used to detect HB S and pre-Core/Core(pre-C/C)coding genes both in one-and two-cell embryos.Reverse transcription-PCR(RT-PCR)analysis was used to study the expression of the two genes.Fluorescence in situ hybridization(FISH)analysis using the full-length HBV DNA as the hybridization probe was performed to confirm the integration of viral DNA in the host embryonic genome.Results:Both HB S and pre-C/C coding genes are present and transcribed in one-and two-cell embryos originated from hamster ova IVF with human spermatozoa carrying HBV DNA sequences.Conclusion:Sperm-mediated HBV genes are able to replicate and express themselves in early embryonic cells.These results provide direct evidence that HBV DNA could transmit vertically to the next generation via the male germ line. 展开更多
关键词 hepatitis B virus gene expression hamster ovary human spermatozoa in vitro fertilization
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Cyclic Changes of Nerve Fibers in Human Endometrium
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作者 Tatsuo Tomita Kuni Mah 《Open Journal of Pathology》 2014年第2期68-78,共11页
Objective: The presence of nerve fibers in human endometrium remains unsettled but recent immunocytochemical studies have shown that there was increased innervation in the endometrium from women with endometriosis and... Objective: The presence of nerve fibers in human endometrium remains unsettled but recent immunocytochemical studies have shown that there was increased innervation in the endometrium from women with endometriosis and some nerve fibers in the normally cycling human endometrium. In the current study, we used uterine tissue cryosections from normal cycling women, which previously provided better immunocytochemical staining for lymphatic vessels than in paraffin sections. Materials and Methods: A total of 16 cases from normally cycling women were included representing menstrual, early proliferative, early to late secretary phase. Neurofilament and CD 56 were used as immunocytochemical markers for nerve fibers with cryosections. Results: There were consistent presence of nerve fibers in myometrium and basalis. Few small nerve fibers were identified in early proliferative endometrium and more nerve fibers were present in lower-half functionalis from mid-secretary phase. Late-secretary functionalis showed less nerve fibers in the upper-half than the lower-half functionalis, implying growing nerve fibers from lower functionalis to upper functionalis in late-secretary phase. Conclusion: Nerve fibers appeared to cyclically grow from basalis to lower functionalis and then from lower functionalis to upper functionalis concomitantly with blood vessels in normally cycling human endometrium. These cycling endometrial nerve fibers consisted mostly of nonmyelinated small nerve fibers, which may transmit pelvic pain in the normally cycling women. 展开更多
关键词 Basalis CD 56 Functionalis human endometrium IMMUNOCYTOCHEMISTRY NERVE NEUROFILAMENT
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Cyclic Changes of Lymphatic Vessels in Human Endometrium
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作者 Tatsuo Tomita Kuni Mah 《Open Journal of Pathology》 2014年第1期5-12,共8页
Objective: The presence of lymphatic vessels in endometrium has been controversial and recent immunocytochemical studies with routinely paraffin embedded sections revealed lymphatic vessels in basalis and occasionally... Objective: The presence of lymphatic vessels in endometrium has been controversial and recent immunocytochemical studies with routinely paraffin embedded sections revealed lymphatic vessels in basalis and occasionally in functionalis. We aimed to investigate endometrial lymphatic vessels by immunocytochemical staining using cryosections, which provided better and consistent immunostaining for lymphatic vessels with a lymphatic marker, D2-40. We aimed further to explore the structure-function relationship of lymphatic vessels in the menstrual cycle. Materials and Methods: Sixteen cases of endometrium from menstrual, early-proliferative to latesecretary phase were immunostained for D2-40 and lymphatic vessels were morphometrically analyzed for functionalis, basalis and myometrium, respectively. Results: Lymphatic vessels were consistently most numerous in myometrium, followed by basalis in all phases whereas menstrual endometrium showed small, fragmented aggregates of lymphatic vessels in thin basalis. Earlyto mid-secretary endometrium revealed many lymphatic vessels in basalis and lower-functionalis with few lymphatic vessels in upper-functionalis. Late-secretary endometrium revealed more lymphatic vessels in upper-functionalis with dilated walls, which then burst at the surface of functionalis. Conclusions: These degenerating lymphatic vessels with markedly dilated lumen in upper-functionalis may contribute to lymphatic leakage in late-secretary phase. These immunostained lymphatic vessels in functionalis support proliferating and degenerating lymphatic vessel cycle synchronized with the menstrual cycle of endometrial arteries to maintain adequate fluid leakage. 展开更多
关键词 Basalis D2-40 human endometrium Funtionalis IMMUNOCYTOCHEMISTRY LYMPHATIC VESSELS
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Cyclic Changes of Lymphatic and Venous Vessels in Human Endometrium
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作者 Tatsuo Tomita Kuni Mah 《Open Journal of Pathology》 2014年第4期194-205,共12页
Context: Cyclic changes of endometrial arteries are well established but possible cyclic changes of lymphatic and venous vessels have not been fully documented. There are no published morphological reports to support ... Context: Cyclic changes of endometrial arteries are well established but possible cyclic changes of lymphatic and venous vessels have not been fully documented. There are no published morphological reports to support cyclic changes of endometrial lymphatic and venous vessels. Objective: Using cryosections of human endometrium, this study aimed to unveil possible cyclic changes of lymphatic and venous vessels. We previously reported cyclic changes of lymphatic vessels in human endometrium using D2-40. Design: A total of 16 cases representing menstrual, proliferative and mid and late secretary phase were studied. For Immunocytochemical staining, lymphatic vessel endothelial hyaluronan receptor 1 and von Willebr and factor were used for lymphatic and venous vessels, respectively. We used polyclonal LYVE-1 in this study, which revealed more lymphatic vessels than using D2-40. Results: Residual lymphatic and venous vessels were present in menstrual basalis. In Day 5 - 9 endometrium, there were sparse lymphatic vessels but were numerous growing venous vessels in thin proliferating functionalis. In Day 14 - 22 endometrium, there were scattered lymphatic vessels and numerous venous vessels in functionalis. In Day 25 - 26 endometrium, there were many dilated lymphatic vessels and numerous dilated, disintegrating venous vessels in upper functionalis than lower functionalis. Conclusion: The above findings support that lymphatic vessels are sparse but venous vessels are numerous in early proliferative functionalis. Lymphatic vessels grow from basalis to thin functionalis. In premenstrual phase, lymphatic vessels proliferate from lower to upper functionalis, and both lymphatic and venous vessels disintegrate for shedding by this immunocytochemical study using lymphatic and venous markers. Thus, all lymphatic, venous and arterial vessels undergo menstrual cyclic changes and shed for menstruation. 展开更多
关键词 Factor-8 human endometrium LYMPHATIC VESSELS LYVE-1 VENOUS VESSELS
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人脐带间充质干细胞培养上清对米非司酮处理的人子宫内膜基质细胞存活、凋亡和子宫内膜容受性的影响
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作者 武梦雪 陈士玲 +4 位作者 刘艳 米旭光 林秀英 付建华 方艳秋 《吉林大学学报(医学版)》 CAS CSCD 北大核心 2024年第1期79-87,共9页
目的:探讨人脐带间充质干细胞培养上清(hUCMSCs-Sup)对米非司酮(Ms)处理的人子宫内膜基质细胞(hEndoSCs)增殖、凋亡和子宫内膜容受性的影响,并阐明其可能的作用机制。方法:体外培养hEndoSCs,分为对照组和40、60、80及100μmol·L-1... 目的:探讨人脐带间充质干细胞培养上清(hUCMSCs-Sup)对米非司酮(Ms)处理的人子宫内膜基质细胞(hEndoSCs)增殖、凋亡和子宫内膜容受性的影响,并阐明其可能的作用机制。方法:体外培养hEndoSCs,分为对照组和40、60、80及100μmol·L-1 Ms组,MTT法检测各组细胞存活率。hEndoSCs分为对照组、40μmol·L-1 Ms组和60μmol·L-1 Ms组,流式细胞术检测各组细胞凋亡率,Western blotting法检测各组细胞中凋亡相关蛋白B细胞淋巴瘤2 (Bcl-2)和Bcl-2相关X蛋白(Bax)蛋白表达水平并计算Bcl-2/Bax比值。hUCMSCs-Sup作用后,hEndoSCs分为对照组、Ms组、Ms+hUCMSCs-Sup组和Ms+hUCMSCs-Sup+3-甲基腺嘌呤(3-MA)组,MTT法检测各组细胞存活率,流式细胞术检测各组细胞凋亡率,Western blotting法检测各组细胞中微管相关蛋白1轻链3B-Ⅱ(LC3B-Ⅱ)和微管相关蛋白1轻链3B-Ⅰ(LC3B-Ⅰ)蛋白表达水平并计算LC3B-Ⅱ/LC3B-Ⅰ比值,实时荧光定量PCR (RT-qPCR)法检测各组细胞中子宫内膜容受性标志分子mRNA表达水平。结果:与对照组比较,40、60、80和100μmol·L-1 Ms组细胞存活率明显降低(P<0.05),且具有时间和剂量依赖性。与对照组比较,40和60μmol·L-1 Ms组细胞凋亡率明显升高(P<0.05),细胞中Bcl-2/Bax比值明显降低(P<0.05)。hUCMSCs-Sup作用后,与对照组比较,Ms组细胞存活率和细胞中LC3B-Ⅱ/LC3B-Ⅰ比值明显降低(P<0.05),细胞凋亡率明显升高(P<0.05),细胞中同源框基因A10 (HOXA10)、白血病抑制因子(LIF)和整合素亚基β3 (ITGB3) mRNA表达水平明显降低(P<0.05);与Ms组比较,Ms+hUCMSCs-Sup组细胞存活率和细胞中LC3B-Ⅱ/LC3B-Ⅰ比值明显升高(P<0.05),细胞凋亡率明显降低(P<0.05),细胞中HOXA10、LIF和ITGb3 mRNA表达水平表达明显升高(P<0.05);与Ms+hUCMSCs-Sup组比较,Ms+hUCMSCs-Sup+3-MA组细胞存活率和细胞中LC3B-Ⅱ/LC3B-Ⅰ比值明显降低(P<0.05)。结论:hUCMSCs-Sup可提高Ms处理后hEndoSCs的存活率,降低其凋亡率,提高子宫内膜容受性,其机制可能与hUCMSCs-Sup激活hEndoSCs自噬有关。 展开更多
关键词 脐带间充质干细胞 人子宫内膜基质细胞 细胞凋亡 细胞自噬 子宫内膜容受性
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聚己内酯-透明质酸静电纺丝膜联合间充质干细胞修复子宫内膜损伤
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作者 安江茹 张锦艺 +3 位作者 王秋花 杨杨杨 王文双 张晓晴 《中国组织工程研究》 CAS 北大核心 2025年第16期3369-3379,共11页
背景:人子宫内膜间充质干细胞能够直接修复受损的子宫内膜,促进血管生成、恢复子宫形态结构,然而将干细胞直接注入受损子宫内膜后的细胞存活率低、滞留时间短,修复效果有限。目的:观察聚己内酯-透明质酸静电纺丝膜复合人子宫内膜间充质... 背景:人子宫内膜间充质干细胞能够直接修复受损的子宫内膜,促进血管生成、恢复子宫形态结构,然而将干细胞直接注入受损子宫内膜后的细胞存活率低、滞留时间短,修复效果有限。目的:观察聚己内酯-透明质酸静电纺丝膜复合人子宫内膜间充质干细胞修复大鼠子宫内膜损伤的效果。方法:①细胞实验:采用胶原酶消化法提取人子宫内膜间充质干细胞,静电纺丝技术制备聚己内酯-透明质酸静电纺丝膜。将人子宫内膜间充质干细胞分别接种于聚苯乙烯培养板与聚己内酯-透明质酸静电纺丝膜上,通过DNA定量分析、WST-1细胞活性实验、鬼笔环肽染色、扫描电镜观察细胞的增殖与黏附能力,qRT-PCR检测静电纺丝膜上细胞CD90、Meflin的mRNA表达。②动物实验:取27只处于动情期的雌性SD大鼠,通过机械搔刮法建立宫腔粘连模型后随机分为3组,每组9只:空白对照组不进行任何治疗,对照组将聚己内酯-透明质酸静电纺丝膜植入宫腔损伤部位,实验组将聚己内酯-透明质酸静电纺丝膜/人子宫内膜间充质干细胞补片植入宫腔损伤部位。术后第3,7,14天取材,采用苏木精-伊红染色观察子宫形态结构及腺体数量,qRT-PCR和免疫荧光染色观察子宫组织CD31、血管内皮生长因子的表达。结果与结论:①细胞实验:与聚苯乙烯培养板相比,聚己内酯-透明质酸静电纺丝膜可促进人子宫内膜间充质干细胞的增殖与黏附,并且聚己内酯-透明质酸静电纺丝膜支持人子宫内膜间充质干细胞基因CD90和Meflin的表达;②动物实验:苏木精-伊红染色显示,聚己内酯-透明质酸静电纺丝膜/人子宫内膜间充质干细胞补片可促进子宫内膜损伤后形态结构的恢复,术后第14天的内膜厚度与腺体数量均多于空白对照组、对照组(P<0.05);qRT-PCR和免疫荧光染色检测显示,实验组术后第7,14天的CD31、血管内皮生长因子mRNA与蛋白表达均高于空白对照组、对照组(P<0.05);③结果表明:聚己内酯-透明质酸静电纺丝膜可以提高干细胞的存活率、延长干细胞与受损组织的接触时间,二者复合移植可更好地修复受损子宫内膜组织。 展开更多
关键词 子宫内膜 宫腔粘连 人子宫内膜间充质干细胞 静电纺丝膜 聚己内酯 透明质酸
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宫腔灌注重组人粒细胞集落刺激因子对改善薄型子宫内膜容受性的影响
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作者 施琼 《当代医学》 2024年第15期56-59,共4页
目的探讨宫腔灌注重组人粒细胞集落刺激因子(rhG-CSF)对改善薄型子宫内膜容受性的影响。方法选取2021年1月至2022年1月景德镇市第二人民医院诊治的94例薄型子宫内膜患者作为研究对象,按照随机数字表法分为观察组与对照组,每组47例。对... 目的探讨宫腔灌注重组人粒细胞集落刺激因子(rhG-CSF)对改善薄型子宫内膜容受性的影响。方法选取2021年1月至2022年1月景德镇市第二人民医院诊治的94例薄型子宫内膜患者作为研究对象,按照随机数字表法分为观察组与对照组,每组47例。对照组给予戊酸雌二醇片治疗,观察组在对照组基础上采用rhG-CSF宫腔灌注治疗,比较两组子宫内膜厚度及形态、子宫血流动力学指标[收缩期峰值流速(PSV)、搏动指数(PI)、阻力指数(RI)]、子宫内膜容受性相关自然杀伤细胞(NK)亚型指标(CD56^(+)CD16^(+)、CD56^(+)CD16^(-))及临床疗效。结果治疗后,两组子宫内膜厚度均厚于治疗前,子宫内膜A型比例均高于治疗前,子宫内膜C型比例均低于治疗前,且观察组子宫内膜厚度厚于对照组,子宫内膜A型比例高于对照组,子宫内膜C型比例低于对照组,差异有统计学意义(P<0.05)。治疗后,两组PSV均快于治疗前,PI、RI均低于治疗前,且观察组PSV快于对照组,PI、RI均低于对照组,差异有统计学意义(P<0.05)。治疗后,两组CD56^(+)CD16^(+)、CD56^(+)CD16^(-)均高于治疗前,且观察组高于对照组,差异有统计学意义(P<0.05)。观察组治疗总有效率为95.74%,高于对照组的82.98%,差异有统计学意义(P<0.05)。结论rhG-CSF宫腔灌注可改善薄型子宫内膜患者子宫内膜厚度、形态、子宫血流动力学指标及子宫内膜容受性。 展开更多
关键词 重组人粒细胞集落刺激因子 宫腔灌注 薄型子宫内膜
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宫腔灌注人粒细胞巨噬细胞刺激因子凝胶对薄型子宫内膜FET结局的影响
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作者 张玉菡 苏琼 +1 位作者 伍琼芳 夏雷震 《宜春学院学报》 2023年第9期45-48,共4页
目的:通过宫腔灌注人粒细胞巨噬细胞刺激因子凝胶探究薄型子宫内膜新型治疗方法,为薄型子宫内膜临床诊疗提供思路和依据。方法:回顾性分析2020年8月1日至2022年8月31日在江西省妇幼保健院辅助生殖中心进行助孕治疗的患者,按照入排标准,... 目的:通过宫腔灌注人粒细胞巨噬细胞刺激因子凝胶探究薄型子宫内膜新型治疗方法,为薄型子宫内膜临床诊疗提供思路和依据。方法:回顾性分析2020年8月1日至2022年8月31日在江西省妇幼保健院辅助生殖中心进行助孕治疗的患者,按照入排标准,将患者分为研究组、对照组,比较两组的数据及其结局。结果:两组对比后发现,研究组患者HCG阳性率(69.93%VS 63.57%)、临床妊娠率(49.65%VS 41.43%)、种植率(39.46%VS 32.56%)、活产率均(40.56%VS 34.29%)高于对照组,但无统计学意义(P>0.05)。结论:研究组患者HCG阳性率、临床妊娠率、种植率、活产率均高于对照组,但无统计学意义,鉴于纳入的研究数量有限,后续还需更多高质量的大样本研究加以探讨。 展开更多
关键词 人粒细胞巨噬细胞刺激因子 薄型子宫内膜 冻融胚胎移植
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人羊膜上皮细胞移植对子宫瘢痕模型大鼠子宫内膜的影响及机制
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作者 王静 路成吉 +3 位作者 臧建新 衣志爽 彭家晟 张峰 《中国比较医学杂志》 CAS 北大核心 2023年第12期21-27,共7页
目的 探讨人羊膜上皮细胞(hAECs)移植对大鼠子宫瘢痕模型子宫内膜的改善及对基质金属蛋白酶8(MMP-8)、血管内皮生长因子(VEGF)的影响。方法 建立大鼠子宫瘢痕模型,并随机分为模型组及移植组,每组各18只,另取18只大鼠作为假手术组。移植... 目的 探讨人羊膜上皮细胞(hAECs)移植对大鼠子宫瘢痕模型子宫内膜的改善及对基质金属蛋白酶8(MMP-8)、血管内皮生长因子(VEGF)的影响。方法 建立大鼠子宫瘢痕模型,并随机分为模型组及移植组,每组各18只,另取18只大鼠作为假手术组。移植组大鼠于子宫瘢痕处注射hAECs,模型组及假手术组仅给予等量PBS。4周后,各组取8只大鼠子宫组织,HE染色和Masson染色分别观察组织形态学变化和纤维化情况,测量子宫内膜厚度和腺体数量;细胞角蛋白和整合素β3免疫组化染色分别评估子宫内膜生长和容受性;RT-qPCR技术检测子宫内膜组织中MMP-8、VEGFA mRNA表达水平;Western blot法检测组织中MMP-8、VEGFA蛋白表达水平;8周后,取各组剩余10只大鼠进行妊娠能力测定。结果 模型组及移植组大鼠子宫内膜厚度、腺体数、角蛋白和整合素β3 IOD值、MMP-8、VEGFA mRNA和蛋白相对表达水平、妊娠率和子宫胚胎数低于假手术组(P<0.05);移植组大鼠子宫内膜厚度、腺体数、角蛋白和整合素β3 IOD值、MMP-8、VEGFA mRNA和蛋白相对表达水平、妊娠率和子宫胚胎数高于模型组(P<0.05),另外,hAECs移植可改善子宫瘢痕大鼠子宫内膜组织病理形态,减轻子宫内膜纤维化程度。结论 hAECs移植可改善子宫内膜损伤,减少瘢痕形成,提高子宫内膜容受性,并增强模型大鼠的妊娠功能,其作用机制可能与促进MMP-8和VEGFA表达有关。 展开更多
关键词 人羊膜上皮细胞 子宫瘢痕 子宫内膜 基质金属蛋白酶8 血管内皮生长因子A
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孕酮、17β-雌二醇对在体培养人子宫内膜出血作用的初步研究
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作者 祝诚 《动物学报》 SCIE CAS 1985年第4期308-312,389,共6页
去卵巢地鼠皮下埋植孕酮和17β-雌二醇硅胶管,两周后在地鼠颊囊内移植分泌期人子宫内膜。手术后第11天用3种不同方式取出含类固醇激素管;1)10只动物仅取出17β-雌二醇管,保留孕酮管,移植内膜均无出血现象;2)31只动物取出孕酮管,保留17β... 去卵巢地鼠皮下埋植孕酮和17β-雌二醇硅胶管,两周后在地鼠颊囊内移植分泌期人子宫内膜。手术后第11天用3种不同方式取出含类固醇激素管;1)10只动物仅取出17β-雌二醇管,保留孕酮管,移植内膜均无出血现象;2)31只动物取出孕酮管,保留17β-雌二醇管,有21只动物移植内膜出血,10只不出血;3)10只动物两种激素管同时取出,所有移植内膜均出血。内膜出血时间比较集中在取管后36—72小时内,占总出血动物的64%。文章讨论了子宫内膜出血原因。 展开更多
关键词 human endometrium. hamster PROGESTERONE
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流行性出血热(EHF)地鼠肾细胞(GHKC)灭活疫苗的初步人体观察 被引量:18
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作者 宋干 黄永成 +13 位作者 杭长寿 郝富勇 李德新 郑晓丽 刘为民 惠连 梁米芳 回良杰 霍子威 李淑兰 张全福 王晓宏 傅增武 王伟 《病毒学报》 CAS CSCD 北大核心 1991年第2期102-106,共5页
用流行性出血热(EHF)病毒L99株(家鼠型)感染金黄地鼠肾细胞(GHKC),经福尔马林(0.025%)灭活制备EHF灭活疫苗(88-17批),经检定批准,肌肉(1ml)接种志愿者12人(IO人于0、7、28天,2人分别于0、7天及0、28天各一针),进行了安全性及抗体反应... 用流行性出血热(EHF)病毒L99株(家鼠型)感染金黄地鼠肾细胞(GHKC),经福尔马林(0.025%)灭活制备EHF灭活疫苗(88-17批),经检定批准,肌肉(1ml)接种志愿者12人(IO人于0、7、28天,2人分别于0、7天及0、28天各一针),进行了安全性及抗体反应的初步人体观察。结果显示,此种疫苗对人体接种无明显不良反应。接种后,12名接种者IFA、ELI-SA及中和抗体(EFRNT)全部阳转,180天和360天后仍分别有10人血清中和抗体阳性。初步表明,此疫苗是安全的,可以有效诱导抗体产生,其中和抗体多数可持续一年以上。 展开更多
关键词 流行性出血热 地鼠肾细胞 灭活疫苗
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人子宫内膜细胞培养及形态学观察 被引量:16
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作者 蒋洲梅 黄玉珠 洪淡华 《生殖与避孕》 CAS CSCD 北大核心 1994年第4期271-274,共4页
13例妇女的子宫内膜组织标本经酶消化分离成腺体及单个细胞后在体外培养。通过光镜及电镜观察可鉴别三种形态特征的细胞,一种为上皮细胞,细胞之间可见连接复合体,免疫组化角蛋白(keratin)染色呈阳性反应。第二种为子宫内... 13例妇女的子宫内膜组织标本经酶消化分离成腺体及单个细胞后在体外培养。通过光镜及电镜观察可鉴别三种形态特征的细胞,一种为上皮细胞,细胞之间可见连接复合体,免疫组化角蛋白(keratin)染色呈阳性反应。第二种为子宫内膜间质细胞。第三种是成纤维细胞。后两种细胞无细胞间连接结构,角蛋白染色均呈阴性反应,而纤维连接素(fibronectin),系间质细胞的一种特异性蛋白质)染色呈阳性。上述三种细胞均能在体外培养并传代。 展开更多
关键词 子宫内膜 细胞培养 形态学
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药物流产后异常子宫出血者子宫内膜中血管生成素1、2的表达 被引量:16
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作者 傅晓敏 王雯 黄丽丽 《解剖学报》 CAS CSCD 北大核心 2007年第6期751-754,共4页
目的研究血管生成素1(Ang-1)和血管生成素2(Ang-2)在药物流产后异常子宫出血者子宫内膜组织中的表达,探讨其与异常子宫出血的关系。方法1087例早孕药物流产后异常出血者的宫腔刮出物行病理学检查;选取40例异常出血者,以20例药物流产后... 目的研究血管生成素1(Ang-1)和血管生成素2(Ang-2)在药物流产后异常子宫出血者子宫内膜组织中的表达,探讨其与异常子宫出血的关系。方法1087例早孕药物流产后异常出血者的宫腔刮出物行病理学检查;选取40例异常出血者,以20例药物流产后无异常出血者作对照,应用免疫组织化学方法检测两组子宫内膜组织中Ang-1、Ang-2的阳性表达率和表达强度的差异。结果1.1087例中有绒毛和蜕膜残留者占80.5%;2.Ang-1、Ang-2蛋白在药物流产后的子宫内膜组织的腺上皮及内膜基质细胞和血管壁中均有表达,以在腺体表达为主;3.两组Ang-1、Ang-2表达率均为100%,但异常出血组Ang-1、Ang-2的表达强度均较对照组高,差异有显著性(P<0.01)。结论药物流产后绒毛和蜕膜的残留可能是导致出血时间延长和出血量多的主要原因;药物流产后异常子宫出血者子宫内膜中血管生成素的表达增强,可能引起血管生成的异常,从而使绒毛和蜕膜不容易脱落或子宫内膜的修复受影响,导致了出血时间的延长。 展开更多
关键词 药物流产 子宫内膜 出血 血管生成素1 血管生成素2 米非司酮 免疫组织化学
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流行性出血热地鼠肾细胞双价灭活疫苗的人体观察 被引量:10
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作者 宋干 黄永成 +16 位作者 杭长寿 李淑兰 刘为民 傅增武 李德新 郝富勇 霍子威 惠连 张全福 回良杰 詹发先 王伟 石晓宏 郑晓丽 林静 王晓宏 王琪 《病毒学报》 CAS CSCD 北大核心 1993年第2期144-151,共8页
为了研制一种对两型流行性出血热(EHF)均有良好预防效果的灭活疫苗,采用地鼠肾细胞(GHKC)传代适应滴度高(≥8.5logTCID50/ml)的两型EHF病毒株(家鼠型L99株及野鼠型JR株)试制出GHKC双价灭活疫苗(90-1批液体苗和90-2批冻干苗),经卫生部批... 为了研制一种对两型流行性出血热(EHF)均有良好预防效果的灭活疫苗,采用地鼠肾细胞(GHKC)传代适应滴度高(≥8.5logTCID50/ml)的两型EHF病毒株(家鼠型L99株及野鼠型JR株)试制出GHKC双价灭活疫苗(90-1批液体苗和90-2批冻干苗),经卫生部批准,进行了约200人的人体免疫观察。疫苗用福尔马林灭话,加AI(OH)_3佐剂。共免疫242人,其中206人免疫前血清抗体反应阴性。全部接种者除极少数有轻、中度反应,余均无不良反应。两批疫苗接种后,IFA抗体阳转率分别为95.9%及89.2%,ELISA抗体分别为93.5%及91.8%。抽样检查中和抗体反应(每批疫苗30人),用酶斑减少中和试验(EFRNT)检查,两批疫苗对L99株病毒中和抗体阳转率均为100%;对JR株抗体阳转率分别为88.2%及66.7%。每个接种者免前及第一针疫苗接种后56,180,360天血清同时用ELISA法检查抗体,并抽样(每批疫苗30例)检查中和抗体,发现两种抗体的阳性率有逐渐降低的动态变化。两批疫苗三种免疫程序(0,7,28天,0,28,42天及0,28天)接种者的免疫反应基本相似,仅0,28天接种者的抗体水平稍低。对68例接种者于免疫后1年用同一疫苗(90-2批)加强免疫一次,其ELISA及中和抗体均显著升高。试用淋巴细胞转化试验检查20人按种后细胞免疫反应。 展开更多
关键词 流行性出血热 地鼠 肾细胞 疫苗
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